Fig 1: Virus oncoproteins E6 and E7 regulate the expression of ESM1 in cervical cancer cells(A) RT-qPCR analysis of the mRNA expression of ESM1 in non-cancer cervical tissues (n = 39) and cervical cancer tissues (n = 69).(B) Representative immunoblotting images on the protein levels of ESM1 in 5 paired of cervical cancer tissues and normal cervical tissues.(C) Levels of ESM1 in serum of healthy controls (n = 52) and cervical cancer patients (n = 116) measured by ELISA.(D) Kaplan-Meier curves for overall survival of 69 patients with cervical cancer according to ESM1 expression.(E) RT-qPCR analysis of relative mRNA level of ESM1 in cervical cancer cell lines (HeLa, SiHa, CaSki, H8 and C33A).(F) Immunoblotting analysis of ESM1 expression in cervical cancer cell lines.(G and H) RT-qPCR analysis and immunoblotting analysis of ESM1 in HPV16 E6-and E7-expressing RPE1 cells were shown. Detection of ESM1 mRNA level and protein level of ESM1 in HeLa cells (I, K) and SiHa cells (J, L) after transfection with siRNAs targeting HPV18 E6, 18 E7 or 16 E6, 16 E7. The protein level of p53 and pRb were examined to evaluate the transfection efficiency of siE6 and siE7, respectively. Data are shown as mean ± SD of three independent experiments (∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001).