Fig 1: Activated microglia in the brain.Histological analysis of the brain. Presence of activated microglia was found (Iba1+ cells in the red circle), but there was not significant difference between R&L stressed mice, regardless of handling, and Sentinels, suggesting that if an inflammatory process occurred in BM, it did not reach the central nervous system. Left: above a x2 magnification of negative control without Iba1 antibody, in the middle a x2 magnification of positive control with Iba1 antibody, below a 4x magnification of PVN (area inside the circle, see methods for stereotaxic coordinates). Center: a representative photomicrograph for each handling group (x5 magnification). Right: a series of images with different degrees of magnification of Iba1+ cells (x20 and x40).
Fig 2: CEFFE attenuates microglia activation and modulate inflammation factors in retina. (A) Representative confocal pictures of sections showing CD11b-positive microglia (green) in retina. (GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer plexiform layer). Scale bar, 50 μm. (B) Quantification of CD11b-positive cells in GCL and entire retina. (C) Quantification of ARG, IL-10, CD206, and TGF-β mRNA expression by RT-qPCR. (D) Quantification of IL-1β, IL-6, iNOS, and TNF-α mRNA expression by RT-qPCR. One-way ANOVA and Tukey’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (n = 5 mice per group).
Supplier Page from Abcam for Microglia Marker (CD11b, CD45, Iba1, TMEM119) Antibody Sampler Panel