UCP2 Antibody PA5-80203 from Thermo Fisher Scientific

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Western blot analysis of UCP2 in Lane 1: rat spleen tissue lysate, Lane 2: rat cardiac muscle tissue lysate, Lane 3: rat brain tissue lysate, Lane 4: mouse spleen tissue lysate, Lane 5: mouse cardiac muscle tissue lysate, Lane 6: mouse brain tissue lysate, Lane 7: human HeLa whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with UCP2 polyclonal antibody (Product # PA5-80203) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000. Signal development was performed using a chemiluminescence (ECL) kit.
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Western blot was performed using Anti-UCP2 Polyclonal Antibody (Product # PA5-80203) and a 28 kDa band corresponding to UCP2 was observed in the cell lines tested. Membrane enriched extracts (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), PC-3 (Lane 3), MCF 10A (Lane 4) and LNCaP (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 ug/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

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UCP2 Antibody

Thermo Fisher Scientific's UCP2 Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Western Blot. The UCP2 Antibody was generated using UCP2, and Uncoupling Protein 2 as the antigen and it reacts with Homo Sapiens, Human, Mouse, and Rat.

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UCP2 Antibody from Thermo Fisher Scientific

Thermo Fisher Scientific

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UCP2 Antibody from Thermo Fisher Scientific

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