Knockdown of MFN2 was achieved by transfecting A549 cells with MFN2 specific siRNA (Silencer® select Product # s19260, & s19261). Western blot analysis (Fig a) was performed using Whole cell extracts from the A549 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blots were probed with Anti-MFN2 Recombinant Rabbit Monoclonal Antibody (Product # 702768, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4,000 dilution). Densitometric analysis of this Western blot is shown in histogram (Fig b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to MFN2