Knockdown of CK2 alpha was achieved by transfecting HeLa cells with CK2 alpha specific validated siRNA (Silencer® select Product # s3638). Western blot analysis (Fig a) was performed using Whole cell extracts from the HeLa knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blots were probed with Anti- CK2 alpha Recombinant Rabbit Monoclonal Antibody (Product # 702811, 1:10,000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). Densitometric analysis of this Western blot is shown in histogram (Fig b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to CK2 alpha.