Fig 1: Kindlin-3 but not talin-1 is important for αMβ2 integrin (Mac-1) clustering. A-I, Representative STORM images of integrin αM on wildtype (WT, A-C), K3-KO (D-F), and TLN1-KO (G-I) differentiated HL60-2 cells without (A, D, G) or with IL-8 (B, E, H) or fMLP (C, F, I) stimulation after clustering analysis using Voronoi diagrams. Adjacent clusters are distinguished by different colors. Non-clustered αM were shown as gray dots. Scale bars are 1 μm. J-M, The number of clusters per WT, K3-KO (J-K), or TLN1-KO (L-M) HL60-2 cell without or with IL-8 (J, L) or fMLP (K, M) stimulation. Means ± SD (n ≥ 50 cells from 3 individual experiments). ns p > 0.05; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 by two-way ANOVA followed by Tukey’s multiple comparison test