For immunofluorescence analysis, SH-SY5Y cells were fixed and permeabilized for detection of endogenous Mitofusin 2 using Mitofusin 2 Recombinant Rabbit Polyclonal Antibody (Product # 711803, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Mitofusin 2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents mitochondrial staining using MitoTracker® Red CMXRos (Product # M7512). Panel d) is a composite image of Panels a, b and c clearly demonstrating co-localization of Mitofusin 2 with mitotracker which specifically binds to the mitochondria. Panel e) represents control cells without primary antibody to assess background. The images were captured at 60X magnification.