Fig 2: Single-Cell Transcriptional Analysis of GBM Cells Reveals a Population of RG-like Cells(A) EMD heatmaps to identify RG-like clusters in the analyses of individual patient data.(B) RG-like cells in individual patient data are segregated into non-cycling (orange) and cycling (green) based on EMD scores for cell-cycle gene sets (top panel). Diffusion maps on raw data using all genes demonstrate the distribution of RG-like cells in individual patients. Each dot represents a cell and each axis represents a diffusion component. The cells are color coded based on the clusters they belong to.(C) Expression distribution of RG markers (HOPX, IL6ST, LIFR, and BLBP) are shown for individual tumors. Orange and blue lines represent the expression of a specific gene in the indicated cluster and its expression in the rest of the data, respectively.(D) Immuno-FISH on sections from four patients show colocalization of pVim with the EGFR probe.(E) CNV analysis of chromosome 7 in RG- and non-RG-like cells (n = minimum of 50 nuclei per tumor). Scale bar, 8 µm.