Mouse Leptin R Biotinylated Antibody BAF497 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse Leptin R Biotinylated Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $599.00Supplier Page View Company Product Page
Catalog NumberBAF497
NCBI Gene Aliasesobl, Leprb, db, OB-RGRP, Modb1, LEPROT, obese-like, Obr, diabetes
ApplicationsBioassay,MACS,Flow Cytomery,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Frozen,In vivo assay,ELISA Detection (Matched Antibody Pair),Immunocytochemistry,Cell Selection
FormatLyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein
TargetLeptin R
ReactivityHuman,Mouse,Transgenic Mouse
HostGoat
IsotypeIgG
Quantity50 ug
Conjugate/TagBiotin
NCBI Full Gene Nameleptin receptor
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse Leptin R, Ala20-Gly839, Accession # Q3US58
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Biotin

Questions and Answers

Can this antibody be used to study leptin receptor expression in flow cytometry?

Yes, the Leptin R Biotinylated Antibody has been utilized in flow cytometry to analyze leptin receptor-expressing cells. For instance, it was used to sort Lin−/LEPR+ fractions in a study, demonstrating its effectiveness in this application ().

Is this antibody effective for detecting leptin receptors in Western blotting?

The Leptin R Biotinylated Antibody has been shown to work in Western blotting, where it was used at a dilution of 1:1,500 to detect leptin receptors in murine samples, confirming its utility in this technique ().

What species can this antibody detect, and is it suitable for human studies?

The Leptin R Biotinylated Antibody is reactive with both mouse and human leptin receptors, making it suitable for studies involving both species ().

Has this antibody been cited in studies related to bone metabolism or obesity?

Yes, the Leptin R Biotinylated Antibody has been cited in multiple studies investigating the role of leptin signaling in obesity and bone metabolism, highlighting its relevance in research focused on these areas (, ).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) A Rat Model Investigation of Enhanced Facial Rejuvenation via PCL Microsphere-Induced Superior Collagen Neogenesis in the Supraperiosteal PlaneAesthetic Plastic SurgeryFebruary 9, 2026Pai-Nien Chu, Shengjun Zhu, Xia Dai, Shihui Zhuobtained from Novus Biologicals (Littleton, CO, USA). Mouse Leptin Receptor (LepR) biotinylated antibody (BAF497) was sourced from R&D systems (Minneapolis, MN, USA). Rat anti-rat endomucin (Emcn) was obtained from

(2) In vivo detection of immune responses via cytokine activity labelingCellFebruary 5, 2026Guangqing Lu, Shanshan Zhang, Mengyang Feng, Eunha Kim, Daniel Cho, Jae Hyun Kim, Hannah Caris, Lev Silberstein, Gloria B. Choi, Jun R. HuhAntibody (1A8),Biolegend,Cat#127616;RRID: AB_1877271 Mouse Leptin R Biotinylated Antibody,R&D System,Cat#BAF497;RRID: RRID: AB_2296953 Mouse IL-17/IL-17A Antibody,R&D Systems,Cat#MAB421;RRID: AB_2125018 IL-17F

(3) DEL‐1 is an Endogenous Senolytic Protein that Inhibits Senescence‐Associated Bone LossAdvanced ScienceJanuary 20, 2026Jong‐Hyung Lim, Kridtapat Sirisereephap, Hui Wang, Giulia Trimaglio, Kyoung‐Jin Chung, Jonathan Korostoff, Tetsuhiro Kajikawa, Xiaofei Li, Meircurius D.C. Surboyo, Tomoki Maekawa, et al.Biolegend), β3 integrin (clone 2C9.G2, catalogue no. 562917, BD Biosciences), LepR‐biotin (catalogue no. BAF497, R&D Systems) for 30 mins at 4 °C. Cells were incubated with Anti‐biotin conjugated with streptavidin

(4) Transpresentation of interleukin 15 by stromal cell subsets regulates immune cell homeostasisFrontiers in ImmunologyJanuary 16, 2026Carmen Stecher, Romana Bischl, Elisabeth Potzmann, Anna Schmid-Böse, Stefanie Ferstl, Nina Braun, Ellen R. Richie, Matthias Farlik, Richard A. Flavell, Dietmar Herndler-Brandstetter(53-6.7, BB700, Cat# 566410) was obtained from BD Biosciences and LepR (polyclonal biotinylated, Cat# BAF497) from R&D Systems. The Zombie Aqua Viability staining kit (BioLegend, Cat# 423101) was used as a viability

(5) Megakaryocytic TGFβ1 orchestrates osteogenesis of LepR+ SSCs to alleviate radiation-induced bone lossExperimental & Molecular MedicineJanuary 14, 2026Yong Tang, Jiulin Tan, Qixiu Yu, Wenxin Yang, Zhengrong Chen, Yueqi Chen, Qiankun Yang, Jie Zhang, Qijie Dai, Bo Yu, et al.and dissociated as described previously16. Cells were stained with an anti-LepR-biotin (R&D System, BAF497) antibody on ice for 30 min, and then the EasySep Release Mouse Biotin Positive Selection Kit (StemCell

(6) Pulsed electromagnetic fields mediate sensory nerve regulation for bone formation in aging modelsNature CommunicationsSeptember 29, 2025Tiantian Wang, Zejun Liang, Changyi Wang, Jiehao Chen, Yaru Ma, Sihan Chen, Dong Zhou, Zhen Hongthe primary antibodies used were anti-CD45-APC (BD Biosciences, 559864, 1:200) and anti-LepR-biotin (BAF497; R&D Systems, 1:200). Flow cytometry was conducted using a 5-laser BD FACS instrument and FACS DIVA

(7) Integration of phospho-signaling and transcriptomics in single cells reveals distinct Th17 cell fatesCell reportsAugust 26, 2025Fortmann SD, Chadha AS, Frey BF, Elahi A, Hanumanthu VS, Liu S, Goldsborough A, Ferrell PB Jr, Grant MB, Weaver CT, et al.(EPR16588),Abcam,ab225261 FITC anti-CD45 (30F11),BioLegend,103107 Biotinylated anti-LEPR (polyclonal),R&D Systems,BAF497 AF+488 anti-rabbit (polyclonal),ThermoFisher,A32790 AF+647 anti-rabbit (polyclonal),ThermoFisher,A32795 AF+488

(8) Regenerating aged bone marrow via a nitric oxide nanopumpNature communicationsJuly 1, 2025Li K, Hu S, Li H, Lin W, Zhao D, Xu Z, Pan C, Wang H, Li D, Liu J, et al.To sort the LepR+ cells, the cell suspensions were incubated with a goat anti-LepR-biotin antibody (BAF497, R&D Systems) for 2 h at 4 °C. The cell suspensions were washed three times with staining buffer and

(9) Heterogeneity of IL-15-expressing mesenchymal stromal cells controls natural killer cell development and immune cell homeostasisNat CommunJuly 1, 2025Carmen Stecher, Romana Bischl, Anna Schmid-Böse, Stefanie Ferstl, Elisabeth Potzmann, Magdalena Frank, Nina Braun, Matthias Farlik, Richard A. Flavell, Dietmar Herndler-Brandstetter1:100). CD8a (53-6.7, Cat# 566410, 1:200) was obtained from BD Biosciences and LepR (polyclonal, Cat# BAF497, 1:67) from R&D Systems. Ghost Dye V510 (Tonbo Biosciences, Cat# SKU 13-0870-T100, 1:200) or Zombie

(10) The immune response against cancer is modulated by stromal cell fibronectinNeoplasiaJune 30, 2025Alexander Lubosch, Lauren Pitt, Caren Zoeller, Franziska Wirth, Tarik Exner, Barbara Steigenberger, Guido Wabnitz, Jutta Schroeder-Braunstein, Inaam A. NakchbandiBiolegend,1:100,AB_571964 Ki67,PE,#652404 Biolegend,1:100,AB_2561525 Leptin receptorStreptavidin,BiotinPerCP-Cy5.5,#BAF497 R&D Systems#405214 Biolegend,1:501:400,AB_2296953AB_2716577 Ly6C,Alexa Fluor 647BV711PerCP-Cy5.5,#128010

(11) Sexual dimorphism in the mouse bone marrow niche regulates hematopoietic engraftment via sex-specific Kdm5c/Cxcl12 signalingJournal of Clinical InvestigationJanuary 21, 2025Xiaojing Cui, Liming Hou, Bowen Yan, Jinpeng Liu, Cuiping Zhang, Pinpin Sui, Sheng Tong, Larry Luchsinger, Avital Mendelson, Daohong Zhou, et al.Cat#: 557909 (APC) or Cat#: 557915 (FITC)), CD31 (Biolegend, Cat#: 102422), Lepr (R&D Systems, Cat#: BAF497), CD51 (BD Biosciences, Cat#: 551187), PDGFRα (Biolegend, Cat#: 135916), Sca-1 (eBioscience, Cat#: 25-5981-82),

(12) Deep imaging of LepR+ stromal cells in optically cleared murine bone hemisectionsBone ResJanuary 13, 2025Yuehan Ni, Jiamiao Wu, Fengqi Liu, Yating Yi, Xiangjiao Meng, Xiang Gao, Luyi Xiao, Weiwei Zhou, Zexi Chen, Peng Chu, et al.,Santa Cruz,sc-18916 Rabbit anti-Leptin Receptor,1:100,Abcam,ab318272 Biotin goat anti-Leptin Receptor,2 ng/μL,R&D Systems,BAF497 Biotin hamster anti-CD3e,1:100,BD Biosciences,51-01082 J Biotin rat anti-CD11b,1:100,BD Biosciences,51-01712

(13) Insights into the metastatic bone marrow niche gained from fibronectin and β1 integrin transgenic miceNeoplasiaDecember 1, 2024Franziska Wirth, Caren Zoeller, Alexander Lubosch, Jutta Schroeder-Braunstein, Guido Wabnitz, Inaam A. Nakchbandi647,100724,Biolegend,1:800 ESAM,APC,136207,Biolegend,1:50 F4/80,PE,123110,Biolegend,1:400 JAM-C,Biotin,Bs-11086R-Biotin,BIOSS,1:50 LepR,Biotin,BAF497,R&D Systems,1:50 Ly6C,Alexa Fluor 647,128010,Biolegend,1:400 Ly6G,PE-Cy7,127618,Biolegend,1:100 Sca-1,FITC,122506,Biolegend,1:800 Sca-1,PE-Cy7,122514,Biolegend,1:200 Ter119,Biotin,116203,Biolegend,1:100 Ter119,Brilliant

(14) KCNB1-Leptin receptor complexes couple electric and endocrine function in the melanocortin neurons of the hypothalamusFASEB journal : official publication of the Federation of American Societies for Experimental BiologyOctober 31, 2024Forzisi-Kathera-Ibarra E, Jo C, Castillo L, Gaur A, Lad P, Bortolami A, Roser C, Venkateswaran S, Dutto S, Selby M, et al.Antibody (GF28R) Biotin,ThermoFisher,MA5-15256-BTIN Mouse Leptin R Biotinylated Antibody,R&D systems,BAF497 IgG (3E8) mouse monoclonal,Santa Cruz Biotechnology,Sc-69786 c-Fos Antibody (E-8),Santa Cruz Biotechnology,sc-166940 Anti-Kv2.1/KCNB1 antibody [K89/34

(15) Itm2a expression marks periosteal skeletal stem cells that contribute to bone fracture healingThe Journal of clinical investigationSeptember 3, 2024Xing W, Feng H, Jiang B, Gao B, Liu J, Xie Z, Zhang Y, Hu X, Sun J, Greenblatt MB, et al.anti-OPN (R&D Systems, AF808, 1:500); mouse anti-Col2a1 (Abcam, Ab185430, 1:200); goat anti-LepR (R&D Systems, BAF497, 1:200); rabbit anti-Ki67 (Abcam, ab15580, 1:200); rabbit anti-Runx2 (Abcam, ab114133, 1:200); mouse

(16) Prevention and treatment of peri-implant fibrosis by functionally inhibiting skeletal cells expressing the leptin receptorNature Biomedical EngineeringJuly 31, 2024Vincentius Jeremy Suhardi, Anastasia Oktarina, Mohammed Hammad, Yingzhen Niu, Qingdian Li, Andrew Thomson, Juan Lopez, Jason McCormick, Ugur M. Ayturk, Matthew B. Greenblatt, et al.catalogue number 740865, lot number 2137890; BD Bioscience), LeptinR (1:100 dilution; catalogue number BAF497, lot number BFV1021071; R&D Systems) and streptavidin-PE (1:500 dilution; catalogue number 554061, lot

(17) PCLAF induces bone marrow adipocyte senescence and contributes to skeletal agingBone ResearchJuly 4, 2024Lingqi Xie, Yalun Cheng, Biao Hu, Xin Chen, Yuze An, Zhuying Xia, Guangping Cai, Changjun Li, Hui PengCell Signaling Technology), p21 (sc-166630, 1:50, Santa Cruz), Osteocalcin (M173, 1:200, Takara), Leptin (BAF497, 1:200, R&D system), Ctsk (57056, 1:300, Cell Signaling Technology). Fluorescence-conjugated secondary

(18) Bone marrow stromal cell-derived hepcidin has antimicrobial and immunomodulatory activitiesScientific reportsFebruary 17, 2024Krepuska M, Mayer B, Vitale-Cross L, Myneni VD, Boyajian MK, Németh K, Szalayova I, Cho T, McClain-Caldwell I, Gingerich AD, et al.

(19) A vertebral skeletal stem cell lineage driving metastasisNatureSeptember 1, 2023Sun J, Hu L, Bok S, Yallowitz AR, Cung M, McCormick J, Zheng LJ, Debnath S, Niu Y, Tan AY, et al.Perilipin (1:200, Abcam, cat. Ab3526), PAX1(1:100, Abcam, cat. Ab252847), LEPR (1:100, R&D systems, cat. BAF497), NESTIN (1:100, Abcam, cat. ab6142), COL2A1 (1:100, Abcam, cat. ab185430); for human samples: HNA (1:200,

(20) Ruxolitinib improves hematopoietic regeneration by restoring mesenchymal stromal cell function in acute graft-versus-host diseaseThe Journal of clinical investigationAugust 1, 2023Lin Y, Gu Q, Lu S, Pan Z, Yang Z, Li Y, Yang S, Lv Y, Zheng Z, Sun G, et al.For primary BMSC injection, cells were stained for biotin-conjugated leptin receptor (R&D Systems, BAF497), CD45 (BioLegend, 30-F11), and Ter119 (BioLegend, TER-119). Streptavidin-APC (BioLegend) was used as

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Leptin R Biotinylated Antibody

Leptin R Biotinylated Antibody

Mouse Leptin R Biotinylated Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

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Mouse Leptin R Biotinylated Antibody from R&D Systems, a Bio-Techne Brand

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