MMP-9 Antibody AF909 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMMP-9 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF909
IsotypeIgG
ReactivityComplex Species Category,Human,Mouse,Transgenic Mouse
HostGoat
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
TargetMMP-9
NCBI Full Gene Namematrix metallopeptidase 9
ApplicationsWB, FCM, ICC, IHC, IHC-fr, IHC-p, IP, EM, Neut
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse MMP-9, Ala20-Pro730, Accession # P41245
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Gene AliasesCLG4B, pro-MMP-9, B/MMP9, MMP-9, GELB, Clg4b, MANDP2, Gel B

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse MMP-9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) MMP-9 is observed and less than 2% cross-reactivity with rhMMP-1, -2, -3, -7, -8, -10, -12, and -13 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study MMP-9 in mouse models of inflammation?

Yes, the MMP-9 antibody has been shown to work effectively in mouse models, as evidenced by its use in studies investigating macrophage responses during sterile inflammation in the skin, where MMP-9 plays a critical role in tissue remodeling and inflammation (see ).

What is the cross-reactivity of this antibody with human MMP-9?

The MMP-9 antibody exhibits approximately 10% cross-reactivity with recombinant human MMP-9, making it suitable for comparative studies between mouse and human samples, although it is primarily designed for mouse applications.

Has this antibody been utilized in immunohistochemistry for tissue samples?

Yes, the MMP-9 antibody has been successfully used in immunohistochemistry, as demonstrated in studies where it was applied to skin tissue sections to analyze MMP-9 expression in various pathological conditions (see ).

What are some specific applications where this antibody has been cited in the literature?

The MMP-9 antibody has been cited in various applications including Western blotting, immunofluorescence, and immunohistochemistry, particularly in studies focusing on macrophage activation and extracellular matrix remodeling in response to inflammatory stimuli (see and ).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Matrix Metalloproteinase‐13 Is an Unfavorable Prognostic Factor in Chordoma by Digesting Growth Inhibitory CollagensCancer MedicineFebruary 19, 2026Yumiko Oishi, Katsuhiro Kawaai, Ryota Tamura, Yukiko Kuroda, Shinobu Noji, Masahiro Toda, Koichi Matsuoab39012, RRID:AB_776416), rabbit anti‐MMP9 (Abcam, ab38898, RRID:AB_776512), goat anti‐MMP9 (R&D Systems, AF909, RRID:AB_355706), mouse CD24 (BD Biosciences, 555426, RRID:AB_395820), and mouse anti‐cathepsin K (DFK,

(2) Sex- and age-dependent neurovascular abnormalities linked to neuroinflammation lead to exacerbated post-ischemic brain injury in Marfan syndrome miceRedox BiolMay 7, 2025Gemma Manich, Belén Pérez, Clara Penas, Ana Paula Dantas, Joana Coutinho, Paula Sánchez-Bernadó, Julián García-Aranda, Juan Fraile-Ramos, Núria Benseny-Cases, Beatriz Martín-Mur, et al.monoclonal anti-Collagen I, 1:1000 (#ab279711, Abcam; Cambridge, UK); goat polyclonal anti-MMP9, 1:1000 (#AF909, R&D Systems; Minneapolis, MN, USA); rabbit monoclonal anti-phospho-SMAD2 1:1000 (#3108, Cell Signaling Technology

(3) Maternal immune activation followed by peripubertal stress combinedly produce reactive microglia and confine cerebellar cognitionCommunications biologyMarch 3, 2025Hikosaka M, Parvez MSA, Yamawaki Y, Oe S, Liang Y, Wada Y, Hirahara Y, Koike T, Imai H, Oishi N, et al.(Invitrogen, 14-9729-82, clone: NRNSTNX, RRID: AB_2848275), Goat anti-MMP-9 Polyclonal antibody (R&D Systems, AF909, RRID: AB_355706), Rabbit anti-MAP2 Polyclonal antibody (abcam, ab32454, RRID: AB_776174), Rat anti-LYVE1

(4) Cysteine Leukotriene Receptor Antagonist—Montelukast—Treatment Improves Experimental Abdominal Aortic Aneurysms in MiceCardiovascular TherapeuticsNovember 27, 2024Kexin Li, Meng Li, Panpan Wei, Kangli Tian, Haole Liu, Weilai Fu, Haiwen Hou, Yajie Chen, Baohui Xu, Yankui Li, et al.Primary Abs against MMP2 (1:200; Cat #, AF1488, R&D Systems, Minneapolis, MN) and MMP9 (1:200; Cat #, AF909, R&D Systems) were also used to measure the expression levels of these two proteins involved in the

(5) Engineered myeloid precursors differentiate into osteoclasts and resorb heterotopic ossification in miceFrontiers in Bioengineering and BiotechnologyNovember 22, 2024Cameron Rementer, Apichai Yavirach, Worakanya Buranaphatthana, Philip A. Walczak, Mei Speer, Kat Pierce, Subramanian Dharmarajan, Elizabeth Leber, Bruce Sangiorzan, Steven Bain, et al.polyclonal antibody (A-11122, Thermo Fisher Scientific) at a 1:200 dilution and goat anti-MMP9 antibody (AF909, Novus, Littleton, CO) at a 1:100 dilution in 2% NDS in PBS at 4°C overnight. Slides were rinsed 3 times

(6) Differences in Acute Expression of Matrix Metalloproteinases-9, 3, and 2 Related to the Duration of Brain Ischemia and Tissue Plasminogen Activator Treatment in Experimental StrokeInternational journal of molecular sciencesAugust 30, 2024Wang D, Saleem S, Sullivan RD, Zhao T, Reed GL.

(7) Metabolic rewiring of macrophages by epidermal-derived lactate promotes sterile inflammation in the murine skinThe EMBO journalApril 1, 2024Ayyangar U, Karkhanis A, Tay H, Afandi AFB, Bhattacharjee O, Ks L, Lee SH, Chan J, Raghavan S.,Novus Biologicals,NBP2-13878,1:300 9.,Laminin 332 (LAM332),Gift by Bob Burgeson,–,1:500 10.,Matrix Metalloproteinase 9 (MMP9),R & D Systems,AF909,1:50 11.,Hypoxia Inducible Factor 1 alpha (HIF1α),Novus Biologicals,NB100-449,1:200 12.,8 hydroxy

(8) Elevated matrix Metalloproteinase-9 associated with reduced cerebellar perineuronal nets in female mice with toxoplasmosisBrain, Behavior, & Immunity - HealthMarch 1, 2024Jianchun Xiao, Jing Huang, Robert H. Yolkenprimary antibodies at 4 °C overnight. Proteins were probed with primary antibodies for MMP-9 (polyclonal, AF909, 1: 800, R&D systems), NGAL (polyclonal, ab63929, 1:500, Abcam), TIMP-1 (polyclonal, AF980, 1:500, R&D systems

(9) Ulmus macrocarpa Hance trunk bark extracts inhibit RANKL-induced osteoclast differentiation and prevent ovariectomy-induced osteoporosis in miceJournal of ethnopharmacologyJanuary 30, 2024Jeong C, Lee CH, Lee Y, Seo J, Wang W, Park KH, Oh E, Cho Y, Park C, Son YJ, et al.

(10) Endothelial LATS2 is a suppressor of bone marrow fibrosisNat Cardiovasc ResJanuary 1, 2024Sivaraj KK, Majev PG, Dharmalingam B, Schröder S, Banjanin B, Stehling M, Zeuschner D, Nordheim A, Schneider RK, Adams RH.rabbit-polyclonal anti-Osterix (Abcam, ab22552, 1:300 dilution), goat polyclonal anti-Mmp9 (R&D Systems, AF909, 1:200 dilution), rabbit polyclonal anti-Col-I (Millipore, AB765P, 1:100 dilution), rabbit polyclonal

(11) HMGB2 is a potential diagnostic marker and therapeutic target for liver fibrosis and cirrhosisHepatology CommunicationsNovember 6, 2023Yi Huang, Suthat Liangpunsakul, Swetha Rudraiah, Jing Ma, Santosh K. Keshipeddy, Dennis Wright, Antonio Costa, Diane Burgess, Yuxia Zhang, Nazmul Huda, et al.α-SMA (#14968). Antibodies against metalloproteinases (MMP)2 (#AF980), MMP9 (#AF1488), and TIMP1 (#AF909) were obtained from R&D Systems (Minneapolis, MN). An anti-HA antibody and an anti-beta actin antibody

(12) HMGB2 is a potential diagnostic marker and therapeutic target for liver fibrosis and cirrhosisHepatol CommunNovember 6, 2023Yi Huang, Suthat Liangpunsakul, Swetha Rudraiah, Jing Ma, Santosh K. Keshipeddy, Dennis Wright, Antonio Costa, Diane Burgess, Yuxia Zhang, Nazmul Huda, et al.α-SMA (#14968). Antibodies against metalloproteinases (MMP)2 (#AF980), MMP9 (#AF1488), and TIMP1 (#AF909) were obtained from R&D Systems (Minneapolis, MN). An anti-HA antibody and an anti-beta actin antibody

(13) Loss of Bone Morphogenetic Protein-9 Reduces Survival and Increases MMP Activity After Myocardial InfarctionJACC. Basic to translational scienceOctober 1, 2023Bhave S, Esposito M, Swain L, Qiao X, Martin G, Wadhwa S, Everett K, Kapur NK.Abcam), periostin (ab14041; Abcam), BMP-9 (MAB3209; R&D Systems), endoglin (AF1320; R&D Systems), MMP-9 (AF909; R&D Systems), MMP2 (AF1488; R&D Systems), Flt-1 (Fms related receptor tyrosine kinase 1) (AF471; R&D Systems

(14) Platelet-derived chemokines promote skeletal muscle regeneration by guiding neutrophil recruitment to injured musclesNature communicationsMay 22, 2023Graca FA, Stephan A, Minden-Birkenmaier BA, Shirinifard A, Wang YD, Demontis F, Labelle M.muscle-infiltrating immune cells, the following antibodies were used: anti-MMP9 (R&D Systems, cat. no. AF909) and anti-Ly6G (BioLegend, cat. no. 17-9668-82) to detect neutrophils; anti-F4/80 (BioLegend, cat. no.

(15) Mineralocorticoid receptor antagonism in diabetes reduces albuminuria by preserving the glomerular endothelial glycocalyxJCI insightMarch 8, 2023Crompton M, Ferguson JK, Ramnath RD, Onions KL, Ogier AS, Gamez M, Down CJ, Skinner L, Wong KH, Dixon LK, et al.antibodies or WGA lectin were incubated overnight at 4°C (MMP2, 10373-2, Proteintech, 1:100; MMP9, AF909, R&D Systems, 1:100; MMP14, EP1264Y, Abcam, 1:1,000; WGA, FITC-WGA, GTX01502, GeneTex, 5 mg/mL, 1:500).

(16) Angiotensin II Type 1 Receptor Blocker Prevents Abdominal Aortic Aneurysm Progression in Osteoprotegerin-Deficient Mice via Upregulation of Angiotensin (1-7)Journal of the American Heart AssociationFebruary 7, 2023Karasaki K, Kokubo H, Bumdelger B, Kaji N, Sakai C, Ishida M, Yoshizumi M.

(17) C-reactive protein deficiency ameliorates experimental abdominal aortic aneurysmsFrontiers in immunologyJanuary 1, 2023Fu Y, Liu H, Li K, Wei P, Alam N, Deng J, Li M, Wu H, He X, Hou H, et al.anti-mouse polyclonal antibodies against MMP2 (1:200, Cat#: AF1488, R&D Systems) and MMP9 (1:200, Cat#: AF909, R&D Systems), a biotinylated goat anti-rat secondary antibody (1:400, Cat#: BA-9400, Vector Laboratories,

(18) NADPH oxidase 1 in chronic pancreatitis-activated pancreatic stellate cells facilitates the progression of pancreatic cancerAmerican journal of cancer researchJanuary 1, 2023Chakraborty A, Halder B, Mondal S, Barrett A, Zhi W, Csanyi G, Sabbatini ME.

(19) Genetic deficiency of protein inhibitor of activated STAT3 suppresses experimental abdominal aortic aneurysmsFrontiers in cardiovascular medicineJanuary 1, 2023Fu W, Liu H, Wei P, Xia C, Yu Q, Tian K, Li Y, Liu E, Xu B, Miyata M, et al.assessed by immunostaining using goat anti-mouse polyclonal antibodies against MMP2 (AF1488) and MMP9 (AF909) (R & D Systems, Minneapolis, MN, United States) (Cite your JIR article). The expression levels were

(20) Regulating microglial miR-155 transcriptional phenotype alleviates Alzheimer's-induced retinal vasculopathy by limiting Clec7a/Galectin-3(+) neurodegenerative microgliaActa neuropathologica communicationsSeptember 8, 2022Shi H, Yin Z, Koronyo Y, Fuchs DT, Sheyn J, Davis MR, Wilson JW, Margeta MA, Pitts KM, Herron S, et al.pAb,Rabbit,1:751:1000,IF- RMVIWB,Thermo Fisher Scientific,51–9000 MMP-9,Goat,1:800,WB,R&D Systems,AF909 Secondary antibody,Secondary antibody,Secondary antibody,Secondary antibody,Secondary antibody,Secondary

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8/4/2016#AF909 Is Good Antibody Timur MavlyutovAim of this research was search for specific anti-MMP-9 antibody. Company recommended this antibody to be specific. To validate it I transfected PC12 cells with MMP9 and confirmed that transfected ... Read Review

Mouse MMP-9 Antibody

MMP-9 Antibody

MMP-9 Antibody

MMP-9 Antibody

MMP-9 Antibody

MMP-9 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (126)

Reviews

MMP-9 Antibody from R&D Systems, a Bio-Techne Brand

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