Bax Antibody AF820 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemBax Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $379.00Supplier Page View Company Product Page
Catalog NumberAF820
ApplicationsImmunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Simple Western,Immunocytochemistry
IsotypeIgG
ReactivityHuman,Mouse,Rat
TargetBax
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose
Quantity30 ug
Conjugate/TagUnconjugated
HostRabbit
ClonalityPolyclonal
ImmunogenKLH-coupled human Bax synthetic peptide
ConcentrationLYOPH
PurityAntigen Affinity-purified
ValidationKnockout Validated
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene NameBCL2 associated X, apoptosis regulator
NCBI Gene AliasesBCL2L4

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human and mouse Bax.

Sizes Available: 30 ug

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Catalpol attenuates postmenopausal osteoporosis by activating SIRT1-mediated P53 deacetylation and inhibiting CASPASE-3-driven osteoblast apoptosisInternational immunopharmacologyJanuary 1, 2026Bian Y, Wang Q, Liang Q, Zhu Y, Wu C, Ruan H, Hu X.

(2) Truncated DAPK Variants Restore Tumor Suppressor Activity and Synergize with Standard Therapies in High-Grade Serous Ovarian CancerCancers (Basel)June 8, 2025Monika Raab, Khayal Gasimli, Balázs Győrffy, Samuel Peña-Llopis, Sven Becker, Mourad Sanhaji, Klaus Strebhardt(1:1000; Cell Signaling, Danvers, MA, USA), Puma (#98672) (1:1000; Cell Signaling, Danvers, MA, USA), Bax (AF820) (1:1000; R&D systems, MN, USA), and HRP-conjugated secondary antibodies (1:5000; GE Healthcare and

(3) S14G-humanin alleviates acute lung injury by inhibiting the activation of NF-κBAgingDecember 4, 2023Wu Y, Zhang H, Guan L, Jia X, Wang M.primary antibodies against SOD2 (1:1000, Cat#sc-137254, Santa Cruz Biotechnology, USA), Bax (1:2000, Cat#AF820, R&D System, USA), Bcl-2 (1: 1000, Cat#AB112, Beyotime Biotechnology, China), cleaved caspase-9 (1:800,

(4) Nerve growth factor receptors in equine synovial membranes vary with osteoarthritic disease severityJournal of orthopaedic research : official publication of the Orthopaedic Research SocietyFebruary 1, 2023Kendall A, Ekman S, Skiöldebrand E.LS‐C389392; Nordic BioSite), p75NTR: 1:2000 (polyclonal, ABIN1917233; Antibodies Online) and BAX: 1:800 (AF820; R&D systems). Rabbit IgG (X0903; Dako) was run in parallel on all sections as a negative isotype control,

(5) Benign prostatic hyperplasia/obstruction ameliorated using a soluble guanylate cyclase activatorThe Journal of pathologyApril 1, 2022Zabbarova IV, Ikeda Y, Kozlowski MG, Tyagi P, Birder LA, Chakrabarty B, Perera SK, Dhir R, Straub AC, Sandner P, et al.tissue sections were used for immunohistochemical detection of Bcl-2 (ab182858, Abcam, 1 μg/ml) and BAX (AF820, R&D Systems, Minneapolis, MN, USA; 1 μg/ml). Primary antibody binding was visualised by sequential

(6) Cyclo(Val-Pro) and Cyclo(Leu-Hydroxy-Pro) from Pseudomonas sp. (ABS-36) alleviates acute and chronic renal injury under in vitro and in vivo models (Ischemic reperfusion and unilateral ureter obstruction)International immunopharmacologyFebruary 1, 2022Hira K, Sharma P, Mahale A, Prakash Kulkarni O, Sajeli Begum A.

(7) Human Umbilical Cord Mesenchymal Stem Cells Attenuate Severe Burn-Induced Multiple Organ Injury via Potentiating IGF-1 and BCL-2/BAX PathwayStem cells internationalJanuary 1, 2022Wang H, Ba T, Wang Q, Yang L, Li C, Hao X, Yin Y, Liu L.

(8) Bone mesenchymal stem cells-derived miR-223-3p-containing exosomes ameliorate lipopolysaccharide-induced acute uterine injury via interacting with endothelial progenitor cellsBioengineeredDecember 1, 2021Liu Y, Zhang S, Xue Z, Zhou X, Tong L, Liao J, Pan H, Zhou S.2275-PC-100, R&D system, USA), cleaved Caspase-3 (1:1500, Catalog AF835, R&D system, USA), Bax (1:2000, AF820, R&D system, USA), Cyclin D1 (1:1500, Catalog MAB4314, R&D system, USA) and CDK2 (1:2000, Catalog AF4654,

(9) Activation of PPAR-β/δ Attenuates Brain Injury by Suppressing Inflammation and Apoptosis in a Collagenase-Induced Intracerebral Hemorrhage Mouse ModelNeurochemical researchApril 1, 2020Tang X, Yan K, Wang Y, Wang Y, Chen H, Xu J, Lu Y, Wang X, Liang J, Zhang X.IL-1β, ab9722; TNF-α, ab1793; Bcl-2, ab182858, GFAP, ab7260, Iba1, ab178847; Abcam, Cambridge, UK; Bax, AF820; R&D Systems, Minneapolis, MN, USA) overnight at 4℃. The membranes were washed with TBST (pH 7.4) and

(10) Influence mechanism of miRNA-144 on proliferation and apoptosis of osteosarcoma cellsOncology lettersFebruary 1, 2020Zhang X, Li Z, Ji W, Chen X, Gao Q, Li D, Qin H.American Thermo Scientific), Bax, caspase-3, Bcl-2 HPR-labeled goat anti-mouse IgG secondary antibody (AF820, AF835, AF810; R&D Systems, Inc.), Annexin V/PI apoptosis detection kit (40302ES20; Shanghai Yu Sheng

(11) A Novel Nanofiber Hydrogel Loaded with Platelet-Rich Plasma Promotes Wound Healing Through Enhancing the Survival of FibroblastsMedical science monitor : international medical journal of experimental and clinical researchNovember 18, 2019Zhang P, Zhou L, Wang L, Dong Q.Canon, Japan). Antibodies were as follows: anti-Bcl-2 (#MAB-8272, 1: 500, R&D System, USA), anti-Bax (#AF820, 1: 1000, R&D System, USA), anti-cyclin D1 (#AF4196, 1: 500, R&D System, USA), and anti-cyclin D3 (#MAB6570,

(12) Lipid storage droplet protein 5 reduces sodium palmitate‑induced lipotoxicity in human normal liver cells by regulating lipid metabolism‑related factorsMolecular medicine reportsAugust 1, 2019Ma X, Cheng F, Yuan K, Jiang K, Zhu T.(Bcl-2; 1:800; cat. no. AF810; R&D Systems, Inc.), Bcl-2-associated X protein (Bax; 1:1,000; cat. no. AF820; R&D Systems, Inc.,), cytochrome c (Cytc; 1:1,000; cat. no. MAB897; R&D Systems, Inc.), Cytc oxidase

(13) Genipin protects against H2O2-induced oxidative damage in retinal pigment epithelial cells by promoting Nrf2 signalingInternational journal of molecular medicineFebruary 1, 2019Zhao H, Wang R, Ye M, Zhang L.R&D Systems, Inc.), anti-B-cell lymphoma-2 (Bcl-2; AF810, 1:800; R&D Systems, Inc.), anti-Bcl-2 associated X (Bax; AF820, 1:1,000; R&D Systems, Inc.) and anti-GAPDH (2275-PC-100, 1:600; R&D Systems, Inc.) at 4°C overnight.

(14) TGF-β1 mediates the effects of aspirin on colonic tumor cell proliferation and apoptosisOncology lettersApril 1, 2018Wang Y, Du C, Zhang N, Li M, Liu Y, Zhao M, Wang F, Luo F.rabbit‑anti‑B‑celllymphoma2 (Bcl‑2; cat. no., AF810), rabbit‑anti‑Bcl‑2‑associated X protein (Bax; cat. no., AF820), rabbit‑anti‑Caspase3 (cat. no., AF‑605‑NA; R&D Systems, Inc.), rabbit‑anti‑Caspase8 (cat. no., 9429;

(15) Lycium barbarum polysaccharide exhibits cardioprotection in an experimental model of ischemia-reperfusion damageMolecular medicine reportsMay 1, 2017Hou YM, Wang J, Zhang XZ.anti‑Bcl‑2 (cat no. #15071, Cell Signalling Technologies, Danvers, MA, USA) and anti‑Bax (cat no. #AF820, R&D Systems, Inc., Minneapolis, MN, USA) antibodies were separately diluted 1:200 and 1:300, respectively,

(16) Bcl-xL Is Essential for the Survival and Function of Differentiated Neurons in the Cortex That Control Complex BehaviorsThe Journal of neuroscience : the official journal of the Society for NeuroscienceMay 18, 2016Nakamura A, Swahari V, Plestant C, Smith I, McCoy E, Smith S, Moy SS, Anton ES, Deshmukh M.(catalog #2586, Cell Signaling Technology), RFP (catalog #600-401-379, Rockland Immunochemicals), Bax (catalog #AF820, R&D Systems), and Bak (catalog #12105, Cell Signaling Technology). Briefly, IHC was performed in the

(17) Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance inductionGutFebruary 1, 2007Karrar A, Broomé U, Uzunel M, Qureshi AR, Sumitran-Holgersson S.

(18) [Ca2+]i reduction increases cellular proliferation and apoptosis in vascular smooth muscle cells: relevance to the ADPKD phenotypeCirculation researchApril 29, 2005Kip SN, Hunter LW, Ren Q, Harris PC, Somlo S, Torres VE, Sieck GC, Qian Q.for cytochrome c (556433), cleaved C9 (552036), and cleaved C3 (551150); and from R&D systems for Bax (AF820). Unless otherwise specified, all reagents were obtained from Sigma. Results ⫹/ⴚ Pkd2 Vessels Have

(19) Sustained early growth response gene 3 expression inhibits the survival of CD4/CD8 double-positive thymocytesJournal of immunology (Baltimore, Md. : 1950)July 1, 2004Xi H, Kersh GJ.

(20) Methamphetamine causes differential regulation of pro-death and anti-death Bcl-2 genes in the mouse neocortexFASEB journal : official publication of the Federation of American Societies for Experimental BiologyAugust 1, 2001Jayanthi S, Deng X, Bordelon M, McCoy MT, Cadet JL.

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Bax Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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Bax Antibody from R&D Systems, a Bio-Techne Brand

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