PTEN Antibody AF847 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemPTEN Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $419.00Supplier Page View Company Product Page
Catalog NumberAF847
NCBI Gene AliasesGLM2, TEP1, PTEN1, MHAM, CWS1, PTENbeta, 10q23del, MMAC1, BZS, DEC
ApplicationsELISA Development,Cell Based ELISA,Immunohistochemistry,Western Blot,Simple Western,Intracellular Staining by Flow Cytometry,CyTOF-ready, ELISA Capture
HostRabbit
ReactivityHuman,Insect - Drosophila,Mouse,Rat
IsotypeIgG
TargetPTEN
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
NCBI Full Gene Namephosphatase and tensin homolog
ClonalityPolyclonal
ImmunogenHuman PTEN synthetic peptide, Ser385-Val403, Accession # P60484
ConcentrationLYOPH
PurityAntigen Affinity-purified
ValidationKnockout Validated
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human, mouse, and rat PTEN in Western blots.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Caulerpin alleviates cyclophosphamide-induced ovarian toxicity by modulating macrophage-associated granulosa cell senescence during breast cancer chemotherapyInternational immunopharmacologyDecember 25, 2024Ren X, Yao B, Zhou X, Nie P, Xu S, Wang M, Li P.

(2) Downregulation of aquaporins and PI3K/AKT and upregulation of PTEN expression induced by the flavone scutellarein in human colon cancer cell linesHeliyonOctober 30, 2024Tarawneh N, Hamadneh L, Alshaer W, Al Bawab AQ, Bustanji Y, Abdalla S.incubated for overnight at 4 °C with the primary antibodies listed below: The antibodies used were PTEN (AF847, R&D System; dilution 1:1000), AKT (MAB2055, R&D System; dilution 1:1000), p-AKT-S473 (AF887, R&D System;

(3) Galaxamide alleviates cisplatin-induced premature ovarian insufficiency via the PI3K signaling pathway in HeLa tumor-bearing miceBMC CancerAugust 28, 2024Huan Tao, Zongbin Chen, Bo Yao, Xinyi Ren, Hanlin Shuai, Shihai Xu, Qingbing Zha, Ping Lianti-p-FOXO3A,Abcam,Cat#ab47285 Rabbit anti-PS6rp,Cell signaling Techology,Cat#4858 Rabbit anti-PTEN,R&D systems,Cat#AF847 List of Antibodies

(4) The Role of PTEN in Chemoresistance Mediated by the HIF-1α/YY1 Axis in Pediatric Acute Lymphoblastic LeukemiaInternational journal of molecular sciencesJuly 16, 2024Antonio-Andres G, Morales-Martinez M, Jimenez-Hernandez E, Huerta-Yepez S.pig serum (2%) and subsequently, the antibodies, anti-PTEN (Novus Biological, Littletown, CO, USA, AF847), anti-NF-κB (Abcam, Cambridge, UK, ab133112), anti- pAkt (Novus Biological, Littletown, CO, USA, AF887),

(5) MicroRNA-21a-5p inhibition alleviates systemic sclerosis by targeting STAT3 signalingJournal of translational medicineApril 1, 2024Park JS, Kim C, Choi J, Jeong HY, Moon YM, Kang H, Lee EK, Cho ML, Park SH.tyrosine 705 (Y705) (Abcam, #ab76315) or serine 727 (S727) (Abcam, #ab30647), and PTEN (R&D Systems, #AF847). After 2 h incubation at room temperature with the above antibodies, the sections were incubated for

(6) Single-Cell Spatial MIST for Versatile, Scalable Detection of Protein MarkersBiosensorsAugust 27, 2023Meah A, Vedarethinam V, Bronstein R, Gujarati N, Jain T, Mallipattu SK, Li Y, Wang J.

(7) Berberine Inhibited Growth and Migration of Human Colon Cancer Cell Lines by Increasing Phosphatase and Tensin and Inhibiting Aquaporins 1, 3 and 5 ExpressionsMolecules (Basel, Switzerland)April 29, 2023Tarawneh N, Hamadneh L, Abu-Irmaileh B, Shraideh Z, Bustanji Y, Abdalla S.Membranes were subsequently incubated overnight with the following primary antibodies at 4 °C: PTEN (AF847, R&D System; 1:1000), p-AKT-S473 (AF887, R&D System; 1:1000), AKT (MAB2055, R&D System; 1:1000), p-mTOR-S2448

(8) Nickel's Role in Pancreatic Ductal Adenocarcinoma: Potential Involvement of microRNAsToxicsMarch 21, 2022Mortoglou M, Manić L, Buha Djordjevic A, Bulat Z, Đorđević V, Manis K, Valle E, York L, Wallace D, Uysal-Onganer P.conditions, with the exception of antibody dilutions (phospho-Akt (AF887, R&D Systems; 1:2000); PTEN (AF847, R&D Systems; 1:1000); PARP (1861790, Thermo-Fisher; 1:1000); FOXO1 (2880S, Cell Signaling; 1:1000);

(9) Role of microRNAs in response to cadmium chloride in pancreatic ductal adenocarcinomaArchives of toxicologyFebruary 1, 2022Mortoglou M, Buha Djordjevic A, Djordjevic V, Collins H, York L, Mani K, Valle E, Wallace D, Uysal-Onganer P.antibody were as follows: phospho-Akt (AF887, R&D Systems; 1:2000); phosphatase and tensin homolog (PTEN) (AF847, R&D Systems; 1:1000); Poly (ADP-ribose) polymerase (PARP) (1861790, Thermo-Fisher; 1:1000); Forkhead

(10) A scalable Drosophila assay for clinical interpretation of human PTEN variants in suppression of PI3K/AKT induced cellular proliferationPLoS geneticsSeptember 1, 2021Ganguly P, Madonsela L, Chao JT, Loewen CJR, O'Connor TP, Verheyen EM, Allan DW.

(11) A scalable Drosophila assay for clinical interpretation of human PTEN variants in suppression of PI3K/AKT induced cellular proliferationPLoS geneticsSeptember 1, 2021Ganguly P, Madonsela L, Chao JT, Loewen CJR, O'Connor TP, Verheyen EM, Allan DW.

(12) Prognostic and Predictive Implications of PTEN in Breast Cancer: Unfulfilled Promises but Intriguing PerspectivesCancersSeptember 19, 2019Carbognin L, Miglietta F, Paris I, Dieci MV.[30],III,MBC,First-line,CT + Trastuzumab + PertuzumabCT + Trastuzumab + Placebo,Overall497,"IHC clone AF847; R and D Systems (modified H-scores: 0–400, for membrane, cytoplasm and nucleus; low versus high: median

(13) Identification of Cross Talk between FoxM1 and RASSF1A as a Therapeutic Target of Colon CancerCancersFebruary 8, 2019Blanchard TG, Czinn SJ, Banerjee V, Sharda N, Bafford AC, Mubariz F, Morozov D, Passaniti A, Ahmed H, Banerjee A.#AF1766), total VEGFR1 (R&D systems, #AF321), total VEGFR2 (R&D systems, #AF357), PTEN (R and D Systems, AF847), FoxM1 (R and D Systems, #AF3975), GAPDH (Sigma Aldrich, St. Louis, MO, USA, #G8795). Images were captured

(14) TGF-β induces phosphorylation of phosphatase and tensin homolog: implications for fibrosis of the trabecular meshwork tissue in glaucomaScientific reportsApril 11, 2017Tellios N, Belrose JC, Tokarewicz AC, Hutnik C, Liu H, Leask A, Motolko M, Iijima M, Parapuram SK.polyacrylamide gels and blotted on to nitrocellulose membranes and probed with anti-PTEN rabbit pAb (AF847) (R&D Systems, Inc), anti-phosphoPTEN Ser380/Thr382/383 rabbit mAb (44A7) (Cell Signaling Technologies,

(15) A novel role for GSK3β as a modulator of Drosha microprocessor activity and MicroRNA biogenesisNucleic acids researchMarch 17, 2017Fletcher CE, Godfrey JD, Shibakawa A, Bushell M, Bevan CL.anti-HA mAb (Covance, 16B12), mouse anti-phospho-serine (Sigma P5747), rabbit anti-PTEN pAb (R+D systems, AF847), rabbit anti-FOXO1 mAb (Abcam, ab52857), rabbit anti-ZEB1 mAb (Cell Signalling, 3396), mouse anti-β-tubulin

(16) Biomarker analysis of the NeoSphere study: pertuzumab, trastuzumab, and docetaxel versus trastuzumab plus docetaxel, pertuzumab plus trastuzumab, or pertuzumab plus docetaxel for the neoadjuvant treatment of HER2-positive breast cancerBreast cancer research : BCRFebruary 9, 2017Bianchini G, Kiermaier A, Bianchi GV, Im YH, Pienkowski T, Liu MC, Tseng LM, Dowsett M, Zabaglo L, Kirk S, et al.factor 1 receptor (IGF1R, Clon 1.004.168, Roche Diagnostics), phosphatase and tensin homolog (PTEN, AF847, R&D Systems, Minneapolis, MN, USA), and pAKT (#3787, Cell Signaling Technology, Danvers, MA, USA) were

(17) Tumor suppressor PTEN in breast cancer: heterozygosity, mutations and protein expressionAnticancer researchMarch 1, 2014Kechagioglou P, Papi RM, Provatopoulou X, Kalogera E, Papadimitriou E, Grigoropoulos P, Nonni A, Zografos G, Kyriakidis DA, Gounaris A.

(18) Basal activation of p70S6K results in adipose-specific insulin resistance in protein-tyrosine phosphatase 1B -/- miceThe Journal of biological chemistryOctober 19, 2007Ruffolo SC, Forsell PK, Yuan X, Desmarais S, Himms-Hagen J, Cromlish W, Wong KK, Kennedy BP.

(19) A dual conditional CRISPR-Cas9 system to activate gene editing and reduce off-target effects in human stem cellsMolecular therapy. Nucleic acidsJanuary 1, 0001"Park SB, Uchida T, Tilson S, Hu Z, Ma CD, Leek M, Eichner M, Hong SG, Liang TJ."

(20) Thymoquinone Inhibits JAK/STAT and PI3K/Akt/ mTOR Signaling Pathways in MV4-11 and K562 Myeloid Leukemia Cells"Pharmaceuticals (Basel, Switzerland)"January 1, 0001"Al-Rawashde FA, Al-Wajeeh AS, Vishkaei MN, Saad HKM, Johan MF, Taib WRW, Ismail I, Al-Jamal HAN.""R&D System, Minneapolis, MN, USA" Anti-phospho-Akt,AF887,"R&D System, Minneapolis, MN, USA" Anti-PTEN,AF847,"R&D System, Minneapolis, MN, USA" List of primary antibodies used for Jess simple western analysis.

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PTEN Antibody

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PTEN Antibody

PTEN Antibody

PTEN Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (20)

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PTEN Antibody from R&D Systems, a Bio-Techne Brand

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