Human HGFR/c-MET Antibody AF276 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman HGFR/c-MET Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF276
ApplicationsBioassay,ELISA Development (Detection),Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Simple Western,Blockade of Receptor-ligand Interaction,Neutralization,Immunocytochemistry,CyTOF-ready, ELISA Capture
Conjugate/TagUnconjugated
ReactivityCanine,Human,Mouse
HostGoat
TargetHGFR/c-MET
IsotypeIgG
Quantity100 ug (also 25 ug)
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant human HGF R/c-MET, Glu25-Thr932, Accession # P08581
ConcentrationLYOPH
PurityAntigen Affinity-purified
NCBI Full Gene NameMET proto-oncogene, receptor tyrosine kinase
NCBI Gene Aliasesc-Met, HGFR, AUTS9, DA11, RCCP2, DFNB97
ValidationKnockout Validated
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this antibody be used for studying the MET receptor in cancer research?

Yes, the HGFR/c-MET antibody has been utilized in various studies related to cancer research. For instance, it was employed in the investigation of receptor cross-interactions in cancer cells, demonstrating its role in cell motility and signaling pathways associated with tumor progression ().

What applications has this antibody been shown to work in?

The HGFR/c-MET antibody has been shown to work in several applications including Western blotting, immunocytochemistry, and immunohistochemistry. It has been specifically cited in studies for detecting MET in various cell types and conditions, such as in the context of epithelial-to-mesenchymal transition and receptor activation (, ).

Is this antibody effective for use in immunofluorescence assays?

Yes, the HGFR/c-MET antibody has been effectively used in immunofluorescence assays. For example, it was utilized at a dilution of 1:100 in studies examining the dynamics of MET receptor activation and its interactions with other receptors in living cells (, ).

What species does this antibody react with?

The HGFR/c-MET antibody is reactive with human and canine species, making it suitable for studies involving these organisms. It has been validated for use in human cell lines and tissues, as well as in canine models ().

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Hepatocyte growth factor may contribute to male protection against pulmonary arterial hypertensionBiology of Sex DifferencesMarch 1, 2026Lejla Medzikovic, Grégoire Ruffenach, Ateyeh Dehghanitafti, Brenda Wong, Ashley Ryder, Mohammad Reza Hatamnejad, Wasila Sun, Leana Esdin, Joshua Eghbali, Adam Brownstein, et al.bovine serum albumin (Sigma #A9647) and incubated with antibodies directed against cMET (R&D Systems #AF276) and GAPDH (Cell Signaling #2118). LI-COR IRDye-conjugated secondary antibodies were used for detection

(2) MET variants with activating N‐lobe mutations identified in hereditary papillary renal cell carcinomas still require ligand stimulationMolecular OncologyFebruary 20, 2025Célia Guérin, Audrey Vinchent, Marie Fernandes, Isabelle Damour, Agathe Laratte, Rémi Tellier, Gabriella O. Estevam, Jean‐Pascal Meneboo, Céline Villenet, Clotilde Descarpentries, et al.performed. The slides were incubated with primary antibodies against MET (R&D System, Minneapolis, MN, USA; AF276 at 0.2 μg·mL−1) and anti‐goat antibody conjugated with Alexa 488 (A11055; Invitrogen). Slides were stained

(3) Single-molecule imaging and molecular dynamics simulations reveal early activation of the MET receptor in cellsNature communicationsNovember 2, 2024Li Y, Arghittu SM, Dietz MS, Hella GJ, Haße D, Ferraris DM, Freund P, Barth HD, Iamele L, de Jonge H, et al.USA) in 1x PBS for 1 h at room temperature with gentle shaking. The primary antibody (goat anti-MET, # AF276, lot CMQ0720032, R&D Systems, USA) was diluted in BB to a final concentration of 2 µg/mL and incubated

(4) Functional interaction between receptor tyrosine kinase MET and ETS transcription factors promotes prostate cancer progressionMolecular OncologyOctober 7, 2024Elisa Carouge, Clémence Burnichon, Martin Figeac, Shéhérazade Sebda, Nathalie Vanpouille, Audrey Vinchent, Marie‐José Truong, Martine Duterque‐Coquillaud, David Tulasne, Anne Chotteau‐LelièvreThen, slides were incubated overnight at 4 °C, with primary antibodies. The goat anti‐Human HGF R/cMET (AF276, R&D Systems, USA) the rabbit anti‐ Phospho‐Met (Tyr1234/1235) (#3126; Cell Signaling Technology, MA,

(5) Dystrophin deficiency impairs cell junction formation during embryonic myogenesis from pluripotent stem cellsiScienceJuly 19, 2024Mozin E, Massouridès E, Mournetas V, Lièvre C, Bourdon A, Jackson DL, Packer JS, Seong J, Trapnell C, Le Guiner C, et al.RRID:AB_10671270 Vimentin eFluor™ 570,Thermo Fisher,Cat# 41-9897-80 RRID:AB_11220476 Human HGFR/c-MET,R&D Systems,Cat# AF276 RRID:AB_355289 Goat anti-Mouse IgG2b Alexa Fluor™ 555,Thermo Fisher,Cat# A-21147 RRID:AB_2535783 Donkey

(6) A tripartite organelle platform links growth factor receptor signaling to mitochondrial metabolismNature communicationsJune 15, 2024Mesa D, Barbieri E, Raimondi A, Freddi S, Miloro G, Jendrisek G, Caldieri G, Quarto M, Schiano Lomoriello I, Malabarba MG, et al.

(7) Knockout of c-Cbl/Cbl-b slows c-Met trafficking resulting in enhanced signaling in corneal epithelial cellsThe Journal of biological chemistryOctober 1, 2023Tarvestad-Laise K, Ceresa BP.Coverslips were incubated for 1 h at room temperature with primary antibodies against c-Met (catalog no.: AF276; R&D Systems) and LAMP1 (catalog no.: 909; Cell Signaling Technologies). After washing, coverslips were

(8) Candida albicans stimulates formation of a multi-receptor complex that mediates epithelial cell invasion during oropharyngeal infectionPLoS pathogensAugust 1, 2023Phan QT, Solis NV, Cravener MV, Swidergall M, Lin J, Huang MY, Liu H, Singh S, Ibrahim AS, Mazzone M, et al.incubated with recombinant c-Met (# MET-H5227, ACROBiosystems) followed by an anti human c-Met antibody (# AF276, R&D systems). The bands were visualized by enhanced chemiluminescence. In parallel 10μg of protein

(9) MET Receptor Tyrosine Kinase Inhibition Reduces Interferon-Gamma (IFN-γ)-Stimulated PD-L1 Expression through the STAT3 Pathway in Melanoma CellsCancersJune 29, 2023Song KY, Han YH, Roehrich H, Brown ME, Torres-Cabala C, Giubellino A.(5% dry milk and 0.1% Tween 20 in Tris-buffered saline) overnight at 4 °C. Immunoblotting with MET (AF276, 0.5 μg/mL, R&D systems, Minneapolis, MN, USA), Phospho-MET (AF2480, 0.5 μg/mL, Tyr1234/1235 or AF3950,

(10) Genetic Ablation of the MET Oncogene Defines a Crucial Role of the HGF/MET Axis in Cell-Autonomous Functions Driving Tumor DisseminationCancersMay 13, 2023Modica C, Cortese M, Bersani F, Lombardi AM, Napoli F, Righi L, Taulli R, Basilico C, Vigna E.ELISA plate was coated with 100 ng/mL of an antibody that recognizes the extracellular domain of MET (AF276—R&D Systems, Minneapolis, MN, USA). After saturation with 0.5% BSA in PBS, cell lysates obtained from

(11) Plexin-B3 expression stimulates MET signaling, breast cancer stem cell specification, and lung metastasisCell reportsMarch 28, 2023Zuo Q, Yang Y, Lyu Y, Yang C, Chen C, Salman S, Huang TY, Wicks EE, Jackson W 3rd, Datan E, et al.

(12) Expression and Prognostic Evaluation of the Receptor Tyrosine Kinase MET in Canine Malignant MelanomaVeterinary sciencesMarch 26, 2023Koo K, Wuenschmann A, Rendahl A, Song KY, Forster C, Wolf-Ringwall A, Borgatti A, Giubellino A.Tween 20 in Tris-buffered saline) overnight at 4 °C. Immunoblotting with MET (R&D systems, Cat. No. AF276), Phospho-MET (Cell Signaling Technology, Danvers, MA, USA; Tyr1234/1235, Cat. No.3077), Phospho-MET

(13) Candida albicans stimulates the formation of a multi-receptor complex that mediates epithelial cell invasion during oropharyngeal infectionbioRxiv : the preprint server for biologyFebruary 23, 2023Phan QT, Solis NV, Cravener MV, Swidergall M, Lin J, Huang MY, Liu H, Singh S, Ibrahim AS, Mazzone M, et al.incubated with recombinant c-Met (# MET-H5227, ACROBiosystems) followed by an anti human c-Met antibody (# AF276, R&D systems). The bands were visualized by enhanced chemiluminescence. In parallel 10μg of protein

(14) The Impact of Nanobody Density on the Targeting Efficiency of PEGylated LiposomesInternational journal of molecular sciencesNovember 29, 2022Mesquita BS, Fens MHAM, Di Maggio A, Bosman EDC, Hennink WE, Heger M, Oliveira S.of the primary antibody diluted 1:30 in blocking buffer (goat anti-human HGFR/c-MET antibody, cat. # AF276, R&D Systems, Minneapolis, MN, USA) for 45 min at room temperature (RT). The cells were washed twice

(15) Cytochalasin B Treatment and Osmotic Pressure Enhance the Production of Extracellular Vesicles (EVs) with Improved Drug Loading CapacityNanomaterials (Basel, Switzerland)December 21, 2021Nair A, Bu J, Rawding PA, Do SC, Li H, Hong S.antibody, SC-15363, Santa Cruz Biotechnology, San Jose, CA, USA) and C-Met (polyclonal anti-c-Met antibody, AF276, R&D Systems, Minneapolis, MN, USA). All the grids were rinsed and floated on 10 nm gold conjugated

(16) Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targetsNature cell biologyDecember 1, 2021Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, et al.(HPA012783) and anti-EGFR (06-847) from Sigma-Aldrich. Anti-GPC-1 (Invitrogen, PA5-28055), anti-MET (AF276) and anti-CD81 (clone 454720; MAB4615) from R&D Systems. Anti-AREG (6R1C2.4) from Bristol-Myers Squibb

(17) The EMT activator ZEB1 accelerates endosomal trafficking to establish a polarity axis in lung adenocarcinoma cellsNature communicationsNovember 3, 2021Banerjee P, Xiao GY, Tan X, Zheng VJ, Shi L, Rabassedas MNB, Guo HF, Liu X, Yu J, Diao L, et al.dilution for immunofluorescence) from Cell Signaling Technologies; and primary antibody against MET (#AF276, 1:100 dilution for immunofluorescence) and recombinant human HGF protein (#294-HG-005/CF) from R&D Systems

(18) RAL GTPases mediate EGFR-driven intestinal stem cell proliferation and tumourigenesiseLifeJune 7, 2021Nászai M, Bellec K, Yu Y, Román-Fernández A, Sandilands E, Johansson J, Campbell AD, Norman JC, Sansom OJ, Bryant DM, et al.555651) for α5β1, anti-CD71 (BDPharmingen, Cat# 555534) for the TfnR, anti-HGFR (R&D Systems, Cat# AF276), and anti-EGFR1 (BDPharmingen, Cat# 555996). Statistical analysis GraphPad Prism 8 software was used

(19) Autocrine signaling by receptor tyrosine kinases in urothelial carcinoma of the bladderPloS oneJanuary 1, 2021Lee YH, Lee MM, De Silva DM, Roy A, Wright CE, Wong TK, Costello R, Olaku O, Grubb RL 3rd, Agarwal PK, et al.from Cell Signaling Technology (Danvers, Massachusetts USA). Antibodies against Met (cat. # BAF358, AF276), RYK (cat. #AF4907), CSF1 (cat. #AF216), CSF1R (cat. #MAB3291) and GAS6 (cat. # AF885) were obtained

(20) Single nuclei RNA-seq of mouse placental labyrinth developmenteLifeNovember 3, 2020Marsh B, Blelloch R.Polyclonal antibody,Abcam,ab51745,IF (1:50) Antibody,Anti-Met Polyclonal antibody,R and D Systems,AF276,IF (1:100) Antibody,Anti-Egfr Polyclonal antibody,R and D Systems,AF1280,IF (1:100) Antibody,Anti-Gata1

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HGFR/c-MET Antibody

HGFR/c-MET Antibody

HGFR/c-MET Antibody

HGFR/c-MET Antibody

HGFR/c-MET Antibody

Human HGFR/c-MET Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

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Human HGFR/c-MET Antibody from R&D Systems, a Bio-Techne Brand

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