Galectin-3BP/MAC-2BP Antibody AF2226 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemGalectin-3BP/MAC-2BP Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF2226
NCBI Gene AliasesM2BP, TANGO10B, MAC-2-BP, BTBD17B, gp90, 90K, CyCAP
ApplicationsImmunohistochemistry,Western Blot,Simple Western,Immunocytochemistry
Quantity100 ug (also 25 ug)
ReactivityHuman,Mouse,Primate - Macaca mulatta (Rhesus Macaque)
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
TargetGalectin-3BP/MAC-2BP
Conjugate/TagUnconjugated
IsotypeIgG
HostGoat
NCBI Full Gene Namegalectin 3 binding protein
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant human Galectin-3BP/MAC-2BP, Val19-Asp585, Accession # Q08380
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human Galectin-3BP/MAC-2BP in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse Galectin-3BP/MAC-2BP is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used for studying galectin-3 binding protein in glioblastoma?

Yes, the Galectin-3BP/MAC-2BP antibody has been shown to be effective in immunohistochemical analysis of patient tissues, indicating that LGALS3BP is highly expressed in glioblastoma multiforme and can serve as a potential disease marker and therapeutic target ().

Is this antibody suitable for use in Western blotting for detecting galectin-3 binding protein?

The Galectin-3BP/MAC-2BP antibody has been utilized in Western blotting to analyze the expression of galectin-3 binding protein in various studies, confirming its effectiveness for this application (, , ).

What species can this antibody detect, and is there any cross-reactivity?

This antibody is reactive with human and non-human primate species, specifically detecting human Galectin-3BP/MAC-2BP with less than 20% cross-reactivity with recombinant mouse Galectin-3BP/MAC-2BP ().

Has this antibody been cited in any significant research studies?

Yes, the Galectin-3BP/MAC-2BP antibody has been cited in at least 16 scientific articles, indicating its relevance and utility in various research contexts, including studies on cancer and protein interactions (, ).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Impact of downstream purification process and AAV serotype on protein-impurity clearanceMolecular Therapy AdvancesJune 11, 2026Nicolas Béard, Grégory Rouby G, Cyril Luc, Coline Thomas, Kloé Vanthournout, Guillaume Dubreuil, Zinédine Loukili, Patrick Santambien, Christine Carapito, Romain Fragnoudwas used at 1:100, the polyclonal goat IgG antibody anti-LG3BP (R&D Systems, Minneapolis, MN, USA, AF2226) was used at 1:50, and the monoclonal mouse IgG1 antibody anti-AAV2 replicase (PROGEN, Heidelberg, Germany,

(2) The Galectin-3-binding protein promotes angiogenesis in pancreatic cancer via simultaneous upregulation of VEGFA and direct HUVEC activation mediated by and VAMP5-STAT3Cell Communication and SignalingMarch 18, 2026Ye Jin Lim, Dong Woo Son, Eun Ji Lee, Chae Won Yu, Yun Ok Oh, Min Jung Cha, Hyori Kim, Kyunggon Kim, Suhwan Changstimulation. rhGal-3BP and recombinant human VEGF (rhVEGF) were obtained from R&D systems (rhGal-3BP; AF2226-GAB, rhVEGF;293-VE). After 7-h incubation, three images were captured for analysis. Tube formation was

(3) Glycosylated LGALS3BP is highly secreted by bladder cancer cells and represents a novel urinary disease biomarkerMolecular OncologyMarch 1, 2026Asia Pece, Giulio Lovato, Ilaria Cela, Arianna Mercatelli, Benedetta Ferro, Jussi Nikkola, Sara Pagotto, Tommaso Grottola, Vincenzo De Laurenzi, Rossella Cicchetti, et al.a goat polyclonal antibody against human LGALS3BP (1 : 400 dilution; 60‐min incubation; catalog no. AF2226; R&D Systems, Minneapolis, MN, USA). Antigen retrieval was carried out by microwave treatment at 750

(4) Evaluation of Antibody–Drug Conjugate Performances Using a Novel HPLC–DAD Method for Tumor-Specific Detection of DM4 and S -Methyl-DM4ACS OmegaOctober 28, 2025Giulio Lovato, Miryam Perrucci, Ilaria Cela, Alessia Lamolinara, Arianna Mercatelli, Vincenzo De Laurenzi, Emily Capone, Marcello Locatelli, Gianluca Salaovernight at 4 °C with the following primary antibodies: antihuman LGALS3BP (goat polyclonal; 1:1000, #AF2226; R&D Systems, MN, USA) and anti-β-actin (mouse monoclonal; 1:40,000, #A5441; Sigma-Aldrich). After three

(5) Evaluation of Antibody–Drug Conjugate Performances Using a Novel HPLC–DAD Method for Tumor-Specific Detection of DM4 and S‑Methyl-DM4ACS OmegaOctober 15, 2025Giulio Lovato, Miryam Perrucci, Ilaria Cela, Alessia Lamolinara, Arianna Mercatelli, Vincenzo De Laurenzi, Emily Capone, Marcello Locatelli, Gianluca Salaovernight at 4 °C with the following primary antibodies: antihuman LGALS3BP (goat polyclonal; 1:1000, #AF2226; R&D Systems, MN, USA) and anti-β-actin (mouse monoclonal; 1:40,000, #A5441; Sigma-Aldrich). After three

(6) Multi-omics reveals that ST6GAL1 promotes colorectal cancer progression through LGALS3BP sialylationBiomolecules and BiomedicineJuly 31, 2025Yuanchao Shi, Zhenzhong Pan, Jingwei Duan, Zexing Wang, Yiliang Fang, Bo Tang, Quanlin Guanthe following antibodies: ST6GAL1 (1:200, AF5924; R&D Systems, MN, USA), hGalectin-3BP (0.2 µg/mL, AF2226; R&D Systems), anti-GAPDH (1:3,000, 2118; Cell Signaling Technology, MA, USA), horseradish peroxidase-conjugated

(7) LGALS3BP antibody-drug conjugate enhances tumor-infiltrating lymphocytes and synergizes with immunotherapy to restrain neuroblastoma growthJournal of translational medicineApril 11, 2025Cela I, Capone E, Pece A, Lovato G, Simeone P, Colasante M, Lamolinara A, Piro A, Iezzi M, Lanuti P, et al.overnight at 4 °C with the following primary antibodies: anti-human LGALS3BP (goat polyclonal; 1:1000, #AF2226; R&D Systems, MN, USA); anti-β-actin (mouse monoclonal; 1:40000, #A5441; Sigma-Aldrich). After three

(8) Potential of Mac-2-binding protein glycan isomer as a new therapeutic target in pancreatic cancerCancer scienceApril 1, 2024Yamanaka T, Araki K, Yokobori T, Muranushi R, Hoshino K, Hagiwara K, Gantumur D, Ishii N, Tsukagoshi M, Watanabe A, et al.

(9) Extracellular LGALS3BP: a potential disease marker and actionable target for antibody-drug conjugate therapy in glioblastomaMolecular oncologyAugust 1, 2023Dufrusine B, Capone E, Ponziani S, Lattanzio R, Lanuti P, Giansanti F, De Laurenzi V, Iacobelli S, Ippoliti R, Mangiola A, et al.Santa Cruz Biotechnology), anti‐Alix (MA1‐83977; Invitrogen, Life Technologies, Carlsbad, CA, USA) and anti‐LGALS3BP (AF2226; R&D Systems, Minneapolis, MN, USA). Dulbecco's Modified Eagle Medium (DMEM), FBS and penicillin–streptomycin

(10) Platelet LGALS3BP as a Mediator of Myeloid Inflammation in Systemic Lupus ErythematosusArthritis & RheumatologyMay 1, 2023Hanane EL El Bannoudi, MacIntosh Cornwell, Elliot Luttrell‐Williams, Alexis Engel, Christina Rolling, Tessa J. Barrett, Peter Izmirly, H. Michael Belmont, Kelly Ruggles, Robert Clancy, et al.blocked with 5% BSA and probed overnight with a primary antibody against human LGALS3BP (R&D SYSTEMS, AF2226) at 4°C. Extensively washed membranes were incubated with HRP-conjugated anti-goat antibody (R&D SYSTEMS,

(11) Proteomics of Primary Uveal Melanoma: Insights into Metastasis and Protein BiomarkersCancersJuly 14, 2021Jang GF, Crabb JS, Hu B, Willard B, Kalirai H, Singh AD, Coupland SE, Crabb JW.(rabbit pAb at 1:2000, #A11787, ABclonal); anti-galectin-3 binding protein (goat pAb at 0.2 µg/mL, #AF2226, R&D Systems, Minneapolis, MN, USA); anti-vitronectin (mouse mAb at 0.4 µg/mL, #MAB2349, R&D Systems);

(12) Targeting Vesicular LGALS3BP by an Antibody-Drug Conjugate as Novel Therapeutic Strategy for NeuroblastomaCancersOctober 15, 2020Capone E, Lamolinara A, Pastorino F, Gentile R, Ponziani S, Di Vittorio G, D'Agostino D, Bibbò S, Rossi C, Piccolo E, et al.°C, subjected to 10% SDS-PAGE and Western blotting using a goat monoclonal antibody against LGALS3BP (AF2226, R&D Systems, Minneapolis, MN, USA) and a mouse human β-actin antibody (Sigma Aldrich Corporation).

(13) Mac-2-binding protein glycan isomer enhances the aggressiveness of hepatocellular carcinoma by activating mTOR signalingBritish journal of cancerSeptember 1, 2020Dolgormaa G, Harimoto N, Ishii N, Yamanaka T, Hagiwara K, Tsukagoshi M, Igarashi T, Watanabe A, Kubo N, Araki K, et al.

(14) Galectin 3-binding protein suppresses amyloid-β production by modulating β-cleavage of amyloid precursor proteinThe Journal of biological chemistryMarch 13, 2020Seki T, Kanagawa M, Kobayashi K, Kowa H, Yahata N, Maruyama K, Iwata N, Inoue H, Toda T.anti-GPLD1 antibody (38A1 ab51356-100, Abcam, Cambridge, UK), goat polyclonal anti-GAL3BP antibody (AF2226, R&D Systems, Minneapolis, MN), rabbit polyclonal anti-BACE1 antibody (ab10716, Abcam), rabbit polyclonal

(15) Schwann Cell Stimulation of Pancreatic Cancer Cells: A Proteomic AnalysisFrontiers in oncologyJanuary 1, 2020Ferdoushi A, Li X, Griffin N, Faulkner S, Jamaluddin MFB, Gao F, Jiang CC, van Helden DF, Tanwar PS, Jobling P, et al.in SC-CM,Antibodies used to detect protein in SC-CM 1,Gal-3BP,Goat polyclonal (1:700),R&D System (AF2226) 2,MMP-2,Goat polyclonal (1:500),R&D System (AF902) 3,Cathepsin D,Goat polyclonal (1:100),R&D System

(16) Quantitative glycoproteomics reveals new classes of STT3A- and STT3B-dependent N-glycosylation sitesThe Journal of cell biologyAugust 5, 2019Cherepanova NA, Venev SV, Leszyk JD, Shaffer SA, Gilmore R.commercial sources. Goat polyclonal antibodies for LG3BP and clusterin were obtained from R&D Systems (AF2226 and AF2937, respectively). Expression of GRP94 in cells was analyzed by protein immunoblotting as described

(17) Lectin, Galactoside-Binding Soluble 3 Binding Protein Promotes 17-N-Allylamino-17-demethoxygeldanamycin Resistance through PI3K/Akt Pathway in Lung Cancer Cell LineMolecular cancer therapeuticsJuly 1, 2017Woo JK, Jang JE, Kang JH, Seong JK, Yoon YS, Kim HC, Lee SJ, Oh SH.

(18) Extracellular matrix proteomics identifies molecular signature of symptomatic carotid plaquesThe Journal of clinical investigationApril 3, 2017Langley SR, Willeit K, Didangelos A, Matic LP, Skroblin P, Barallobre-Barreiro J, Lengquist M, Rungger G, Kapustin A, Kedenko L, et al.

(19) M2BP inhibits HIV-1 virion production in a vimentin filaments-dependent mannerScientific reportsSeptember 8, 2016Wang Q, Zhang X, Han Y, Wang X, Gao G.(Cell Signaling Technology, catalogue no. 2368P), M2BP-specific antibody (R&D Systems, catalogue no. AF2226), gp120-specific antibody (Abcam, catalogue no. ab21179), gp41-specific antibody (Santa Cruz Biotechnology,

(20) Human galectin 3 binding protein interacts with recombinant adeno-associated virus type 6Journal of virologyJune 1, 2012Denard J, Beley C, Kotin R, Lai-Kuen R, Blot S, Leh H, Asokan A, Samulski RJ, Moullier P, Voit T, et al.

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