Granzyme B Antibody AF1865 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemGranzyme B Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAF1865
NCBI Gene AliasesCtla-1, GZB, Ctla1, CCP-1/C11, CCP1
ApplicationsWestern Blot, Immunohistochemistry, Immunoprecipitation, Neutralization, Immunocytochemistry, ELISA Capture (Matched Antibody Pair)
ReactivityMouse
HostGoat
Quantity100 ug (also 25 ug)
IsotypeIgG
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
TargetGranzyme B
NCBI Full Gene Namegranzyme B
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse Granzyme B, Lys17-Ser247, Accession # P04187
Brand/TrademarkR&D Systems, a Bio-Techne Brand
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Specificity: Detects mouse Granzyme B in ELISAs and Western blots

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Impaired regulatory function of granzyme B-producing B cells against T cell inflammatory responses in lupus miceLupus Science & MedicineJuly 27, 2023Jimeng Xue, Liling Xu, Hua Zhong, Mingxin Bai, Xin Li, Ranran Yao, Ziye Wang, Zhen Zhao, Hongchao Li, Huaqun Zhu, et al.and samples were collected for further analyses. Antibodies and reagents Mouse GrB Antibody (Cat# AF1865), Normal Goat IgG Control (Cat# AB-108-C) and the Mouse GrB ELISpot Development Module (Cat# SEL1865)

(2) Cationic-nanogel nasal vaccine containing the ectodomain of RSV-small hydrophobic protein induces protective immunity in rodentsnpj VaccinesJuly 24, 2023Shingo Umemoto, Rika Nakahashi-Ouchida, Yoshikazu Yuki, Shiho Kurokawa, Tomonori Machita, Yohei Uchida, Hiromi Mori, Tomoyuki Yamanoue, Takehiko Shibata, Shin-ichi Sawada, et al.anti-mouse IFNγ (#551216, Clone R4-6A2, BD Biosciences, NJ, USA) or 5 μg/mL anti-mouse Granzyme B (#AF1865, R&D Systems, MN, USA) overnight at 4 °C. Plates were washed 3 times with PBS and blocked with complete

(3) Preclinical PET Imaging of Granzyme B Shows Promotion of Immunological Response Following Combination Paclitaxel and Immune Checkpoint Inhibition in Triple Negative Breast CancerPharmaceuticsFebruary 18, 2022Napier TS, Hunter CL, Song PN, Larimer BM, Sorace AG.expression. Primary antibodies for anti-granzyme B (dilution 1:100, R&D Systems, Minneapolis, MN, cat. AF1865) was incubated overnight at 4 °C. Negative controls for the immunostaining procedure were conducted

(4) ST3GAL2 knock-down decreases tumoral character of colorectal cancer cells in vitro and in vivoAmerican journal of cancer researchJanuary 1, 2022Deschuyter M, Leger DY, Verboom A, Chaunavel A, Maftah A, Petit JM.

(5) RNF144A deficiency promotes PD-L1 protein stabilization and carcinogen-induced bladder tumorigenesisCancer LettersNovember 1, 2021Shiuh-Rong Ho, Yu-Cheng Lee, Michael M. Ittmann, Fang-Tsyr Lin, Keith Syson Chan, Weei-Chin Linactive Caspase-3 (Cell signaling, #9661, 1:200), Ki-67 (Abcam, ab16667, 1:100), granzyme B (R&D systems, AF1865, 1:200), and CD8 (Cell signaling, #98941, 1:200). Immunofluorescence analysis was performed as described

(6) DDX3 modulates the tumor microenvironment via its role in endoplasmic reticulum-associated translationiScienceSeptember 24, 2021Chen HH, Yu HI, Rudy R, Lim SL, Chen YF, Wu SH, Lin SC, Yang MH, Tarn WY.RRID:AB_1562699 EIF2α,Santa Cruz,Cat#sc-133132 GAPDH,ABclonal,Cat#AC002; RRID:AB_2736879 Granzyme B,R&D Systems,Cat#AF1865; RRID:AB_2294988 E-cadherin,BD Biosciences,Cat#610181; RRID:AB_397580 Lamin A/C,Merck,Cat#SAB4200236;

(7) CD8⁺ tissue-resident memory T cells promote liver fibrosis resolution by inducing apoptosis of hepatic stellate cellsNature communicationsJuly 22, 2021Koda Y, Teratani T, Chu PS, Hagihara Y, Mikami Y, Harada Y, Tsujikawa H, Miyamoto K, Suzuki T, Taniki N, et al.anti-mouse Fas antibody (1/100, R& D systems, AF435), anti-mouse Granzyme B antibody (1/100, R& D systems, AF1865), and anti-mouse Perforin antibody (1/100, Abcam, Ab16074) were used as primary antibody. After washing

(8) circFAT1 Promotes Cancer Stemness and Immune Evasion by Promoting STAT3 ActivationAdvanced ScienceJuly 1, 2021Lingfei Jia, Yilun Wang, Cun‐Yu WangCat#9661; 1:200), anti‐CD8 (Cell Signaling Technology Cat#98941; 1:200), anti‐Granzyme B (R&D Systems Cat#AF1865; 1:100), and anti‐CXCL10 (R&D Systems Cat#MAB466; 1:100). The signals were detected using secondary

(9) Targeting KDM4A epigenetically activates tumor-cell-intrinsic immunity by inducing DNA replication stressMolecular cellMay 20, 2021Zhang W, Liu W, Jia L, Chen D, Chang I, Lake M, Bentolila LA, Wang CY.CXCR3-173),BioXCell,Cat# BE0249; RRID: AB_2687730 Goat polyclonal anti-Granzyme B,NOVUS Biologicals,Cat#AF1865; RRID: AB_2294988 Rabbit polyclonal anti-FOSL1,Cell Signaling Technology,Cat#5281S; RRID: AB_10557418 Rabbit

(10) Novel Combinatorial MicroRNA-Binding Sites in AAV Vectors Synergistically Diminish Antigen Presentation and Transgene Immunity for Efficient and Stable TransductionFrontiers in immunologyJanuary 1, 2021Muhuri M, Zhan W, Maeda Y, Li J, Lotun A, Chen J, Sylvia K, Dasgupta I, Arjomandnejad M, Nixon T, et al.PBS. The slides were then stained for CD8 (1:500, D4W2Z, Cell Signaling Technology), granzyme B (1:40, AF1865, R&D Systems), F4/80 (1:100, MCA497R, Bio-Rad), and OVA (1:500, AB1225, Millipore Sigma) for 16 hours

(11) Immune Remodeling of the Extracellular Matrix Drives Loss of Cancer Stem Cells and Tumor RejectionCancer immunology researchDecember 1, 2020Pires A, Greenshields-Watson A, Jones E, Smart K, Lauder SN, Somerville M, Milutinovic S, Kendrick H, Hindley JP, French R, et al.used as follows: anti-TNC (ab108930, abcam) 1 μg/mL, anti-CD3 (GA50361-2, Agilent) 2 μg/mL, anti-GZMB (AF1865, R&D Systems) 0.4 μg/mL, anti-CD8 (42-0081-82, ThermoFisher) 1 mg/mL, anti-Lumican (AF2745, R&D Systems)

(12) Early T cell infiltration is modulated by programed cell death-1 protein and its ligand (PD-1/PD-L1) interactions in murine kidney transplants.Kidney InternationalOctober 1, 2020Young Jun Shim, Raneem Khedraki, Jayeeta Dhar, Ran Fan, Nina Dvorina, Anna Valujskikh, Robert L. Fairchild, William M. Baldwin(ab217344), LCN2 (ab70287), PD-1 (ab214421) and Ki-67 (ab16667) from Abcam (Cambridge, MA), granzyme B (AF1865) and PD-L1 (rabbit MAB 90781; clone 2096C from R&D Systems, Minneapolis, MN). The primary antibody to

(13) BMI1 Inhibition Eliminates Residual Cancer Stem Cells after PD1 Blockade and Activates Antitumor Immunity to Prevent Metastasis and RelapseCell stem cellAugust 6, 2020Jia L, Zhang W, Wang CY.Cat#9661; 1:200), anti-CD8 (Cell Signaling Technology Cat#98941; 1:200), anti-Granzyme-B, (R&D Systems, Cat#AF1865; 1:100), and anti-S100 (Abcam Cat#ab4066; 1:200). The immunocomplexes were detected and visualized using

(14) IL-15, gluten and HLA-DQ8 drive tissue destruction in coeliac diseaseNatureFebruary 1, 2020Abadie V, Kim SM, Lejeune T, Palanski BA, Ernest JD, Tastet O, Voisine J, Discepolo V, Marietta EV, Hawash MBF, et al.

(15) The let-7 family of microRNAs suppresses immune evasion in head and neck squamous cell carcinoma by promoting PD-L1 degradationCell communication and signaling : CCSDecember 27, 2019Yu D, Liu X, Han G, Liu Y, Zhao X, Wang D, Bian X, Gu T, Wen L.Inc., MA, USA), CD8 (ab22378, Rat, dilution ratio of 1: 200, Abcam, Cambridge, USA) and Granzyme b (AF1865, Goat, dilution ratio of 1: 100, R&D Systems, R&D Systems, Minneapolis, MN, USA). Immunohistochemistry

(16) IL-6/JAK1 pathway drives PD-L1 Y112 phosphorylation to promote cancer immune evasionThe Journal of clinical investigationJuly 15, 2019Chan LC, Li CW, Xia W, Hsu JM, Lee HH, Cha JH, Wang HL, Yang WH, Yen EY, Chang WC, et al.

(17) PARP Inhibitor Efficacy Depends on CD8⁺ T-cell Recruitment via Intratumoral STING Pathway Activation in BRCA-Deficient Models of Triple-Negative Breast CancerCancer discoveryJune 1, 2019Pantelidou C, Sonzogni O, De Oliveria Taveira M, Mehta AK, Kothari A, Wang D, Visal T, Li MK, Pinto J, Castrillon JA, et al.

(18) Metformin Promotes Antitumor Immunity via Endoplasmic-Reticulum-Associated Degradation of PD-L1Molecular cellAugust 16, 2018Cha JH, Yang WH, Xia W, Wei Y, Chan LC, Lim SO, Li CW, Kim T, Chang SS, Lee HH, et al.Cell Signaling Technology, Danvers, MA, USA ), CD8 (MCA609G, 1:100; BioRad, Hercules, CA, USA), GranzymeB (AF1865, 1:500; R&D Systems, Minneapolis, MN, USA), PD-L1 (13684, 1:200; Cell Signaling Technology), HSP90B1

(19) Juxtacrine Signaling Inhibits Antitumor Immunity by Upregulating PD-L1 ExpressionCancer researchJuly 15, 2018Yang WH, Cha JH, Xia W, Lee HH, Chan LC, Wang YN, Hsu JL, Ren G, Hung MC.(1:300; Cell Signaling Technology #9661L), CD8 (1:100; BioRad #MCA609G), GranzymeB (1:500; R&D Systems #AF1865), PD-L1 (1:200; Cell Signaling Technology #13684), and phalloidin (ThermoFisher #A12379) overnight at

(20) STT3-dependent PD-L1 accumulation on cancer stem cells promotes immune evasionNature communicationsMay 15, 2018Hsu JM, Xia W, Hsu YH, Chan LC, Yu WH, Cha JH, Chen CT, Liao HW, Kuo CW, Khoo KH, et al.abcam, #ab22378, 1:200), and granzyme b (marker for activated cytotoxic T lymphocyte; R&D Systems, #AF1865, 1:100). To study the therapeutic effect of etoposide and/or Tim-3 antibody in preclinical tumor models,

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Cell communication and signaling : CCS

Granzyme B Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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Granzyme B Antibody from R&D Systems, a Bio-Techne Brand

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