XIAP Antibody AF8221 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemXIAP Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF8221
NCBI Gene AliasesXLP2, MIHA, hIAP3, API3, hIAP-3, ILP1, BIRC4, IAP-3
ApplicationsFlow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Frozen,Simple Western,Immunocytochemistry
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman,Mouse,Rat
TargetXIAP
Quantity100 ug (also 25 ug)
HostGoat
Conjugate/TagUnconjugated
IsotypeIgG
NCBI Full Gene NameX-linked inhibitor of apoptosis
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human XIAP, Met1-Ser497 (Ser162Cys), Accession # P98170
ConcentrationLYOPH
PurityAntigen Affinity-purified
ValidationKnockout Validated
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human, mouse and rat XIAP in Western blots.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) BIRC5 Promoter-Driven Nanodrugs Suppress BIRC5-Positive Cancers Independent of ABCB1 Status and IDO1 ExpressionInt J NanomedicineApril 28, 2026Yung-Chieh Chang, Wei-Lun Huang, Wu-Chou Su, Euphemia Leung, Fong-Yu Cheng, Chun Hei Antonio Cheungcat. no. AF886), anti-ACTA1 (Merck Millipore, cat. no. MAB1501), anti-BIRC4 (R&D Systems, cat. no. AF8221), anti–γ-H2AX (Merck Millipore, cat. no. 05–636), anti-CASP3 (GeneTex, cat. no. GTX110543), anti-PARP

(2) Inhibition of lung tumorigenesis by transient reprogramming in cancer cellsCell death & diseaseNovember 25, 2024Pedrosa P, Zhang Z, Nuñez-Quintela V, Macias D, Ge J, Denholm M, Dyas A, Estevez-Souto V, Lado-Fernandez P, Gonzalez P, et al.beta-actin: 4967, Cell Signaling Technology, 1:3,000; Oct4: 2840, Cell Signaling Technology, 1:100; Xiap: AF8221, Bio-Techne, 1:100; Beta-Tubulin: 2146, Cell Signaling Technology, 1:5,000; p21 Waf1/Cip1(12D1): 2947,

(3) Identification of a New Role of miR-199a-5p as Factor Implied in Neuronal Damage: Decreasing the Expression of Its Target X-Linked Anti-Apoptotic Protein (XIAP) After SCIInternational journal of molecular sciencesNovember 18, 2024Muñoz-Galdeano T, Reigada D, Soto A, Barreda-Manso MA, Ruíz-Amezcua P, Nieto-Díaz M, Maza RM.

(4) cIAP1/TRAF2 interplay promotes tumor growth through the activation of STAT3OncogeneJanuary 1, 2023Dumétier B, Zadoroznyj A, Berthelet J, Causse S, Allègre J, Bourgeois P, Cattin F, Racoeur C, Paul C, Garrido C, et al.

(5) Nociceptor-derived Reg3γ prevents endotoxic death by targeting kynurenine pathway in microgliaCell reportsMarch 8, 2022Sugisawa E, Kondo T, Kumagai Y, Kato H, Takayama Y, Isohashi K, Shimosegawa E, Takemura N, Hayashi Y, Sasaki T, et al.

(6) Death agonist antibody against TRAILR2/DR5/TNFRSF10B enhances birinapant anti-tumor activity in HPV-positive head and neck squamous cell carcinomasScientific reportsMarch 18, 2021An Y, Jeon J, Sun L, Derakhshan A, Chen J, Carlson S, Cheng H, Silvin C, Yang X, Van Waes C, et al.(R&D Systems, AF8181), polyclonal anti-cIAP-2 (R&D Systems, AF8171), polyclonal anti-XIAP (R&D Systems, AF8221), and polyclonal anti-FADD (Cell Signaling Technology, 2782). Whole-cell lysates were collected and

(7) Alternol Sensitizes Renal Carcinoma Cells to TRAIL-Induced ApoptosisFrontiers in pharmacologyJanuary 1, 2021Ren Y, Wang X, Huang S, Xu Y, Weng G, Yu R.1:1,000) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies against XIAP (cat no AF8221; dilution 1:1,000) and survivin (cat no AF886; dilution 1:1,000) were purchased from R&D Systems (Minneapolis,

(8) Tamoxifen Rechallenge Decreases Metastatic Potential but Increases Cell Viability and Clonogenicity in a Tamoxifen-Mediated Cytotoxicity-Resistant Subline of Human Breast MCF7 Cancer CellsFrontiers in cell and developmental biologyJanuary 1, 2020Chang YC, Cheung CHA, Kuo YL.United States, cat# GTX102965); anti-XIAP (R&D System, Minneapolis, Minnesota, United States, cat# AF8221); anti-Bcl-2 (Genetex, Irvine, California, United States, cat# 100064); anti-Smac/Diablo (Merck Millipore,

(9) Dual Antagonist of cIAP/XIAP ASTX660 Sensitizes HPV(-) and HPV(+) Head and Neck Cancers to TNFα, TRAIL, and Radiation TherapyClinical cancer research : an official journal of the American Association for Cancer ResearchNovember 1, 2019Xiao R, An Y, Ye W, Derakhshan A, Cheng H, Yang X, Allen C, Chen Z, Schmitt NC, Van Waes C.primary antibodies were used for Western blots: anti-cIAP1 (AF8181), anti-cIAP2 (AF8171), anti-XIAP (AF8221), anti-MDM2 (AF1244, R&D Systems); anti-caspase 8 (9746S), anti-caspase 3 (9662S), anti-p21 (2947S),

(10) ASTX660, a Novel Non-peptidomimetic Antagonist of cIAP1/2 and XIAP, Potently Induces TNFα-Dependent Apoptosis in Cancer Cell Lines and Inhibits Tumor GrowthMolecular cancer therapeuticsJuly 1, 2018Ward GA, Lewis EJ, Ahn JS, Johnson CN, Lyons JF, Martins V, Munck JM, Rich SJ, Smyth T, Thompson NT, et al.

(11) Chronic oxycodone induces axonal degeneration in rat brainBMC neuroscienceMarch 23, 2018Fan R, Schrott LM, Arnold T, Snelling S, Rao M, Graham D, Cornelius A, Korneeva NL.Cell Signaling, 4019); phospho-tau (dilution 1:1000; Cell Signaling, 5383); XIAP (dilution 1:3000; R&D Systems, AF8221). The membranes were incubated with primary antibodies in 5% BSA in buffer TBS-T [20 mM Tris–HCl, 150

(12) Anticancer efficacy of the hypoxia-activated prodrug evofosfamide is enhanced in combination with proapoptotic receptor agonists against osteosarcomaCancer medicineSeptember 1, 2017Liapis V, Zysk A, DeNichilo M, Zinonos I, Hay S, Panagopoulos V, Shoubridge A, Difelice C, Ponomarev V, Ingman W, et al.

(13) Delayed apoptosis allows islet β-cells to implement an autophagic mechanism to promote cell survivalPloS oneJanuary 1, 2017Hayes HL, Peterson BS, Haldeman JM, Newgard CB, Hohmeier HE, Stephens SB.(Cell Signaling Technology Cat#2775), PARP (Cell Signaling Technology Cat# 9542, RRID:AB_2160739), XIAP (R&D Systems Cat#AF8221), and γ-tubulin (Sigma-Aldrich Cat# T6557, RRID:AB_477584). Primary antibodies were detected using goat

(14) USP9X stabilizes XIAP to regulate mitotic cell death and chemoresistance in aggressive B-cell lymphomaEMBO molecular medicineAugust 1, 2016Engel K, Rudelius M, Slawska J, Jacobs L, Ahangarian Abhari B, Altmann B, Kurutz J, Rathakrishnan A, Fernández-Sáiz V, Brunner A, et al.BD Biosciences #610716), XIAP (1:1,000, rabbit, Cell Signaling #2042), and XIAP (1:1,000, R&D Systems #AF8221). Immunofluorescence For immunofluorescence microscopy, cells were grown on cover slips prior to fixation

(15) Pro-Apoptotic Activity of New Honokiol/Triphenylmethane Analogues in B-Cell Lymphoid MalignanciesMolecules (Basel, Switzerland)July 30, 2016Mędra A, Witkowska M, Majchrzak A, Cebula-Obrzut B, Bonner MY, Robak T, Arbiser JL, Smolewski P.

(16) Delivery of a survivin promoter-driven antisense survivin-expressing plasmid DNA as a cancer therapeutic: a proof-of-concept studyOncoTargets and therapyJanuary 1, 2016Lin KY, Cheng SM, Tsai SL, Tsai JY, Lin CH, Cheung CH.overnight at 4°C with different primary antibodies ([anti-survivin, anti-XIAP, and anti-AIF; cat# AF886, AF8221, and AF1457, R&D systems, Minneapolis, MN, USA], [anti-actin and anti-γH2AX; cat# MAB1501 and 05636,

(17) Doxorubicin overcomes resistance to drozitumab by antagonizing Inhibitor of Apoptosis Proteins (IAPs)Anticancer researchDecember 1, 2014Zinonos I, Labrinidis A, Liapis V, Hay S, Panagopoulos V, Denichilo M, Ponomarev V, Ingman W, Atkins GJ, Findlay DM, et al.

(18) Cytotoxic activity of the amphibian ribonucleases onconase and r-amphinase on tumor cells from B cell lymphoproliferative disordersInternational journal of oncologyJuly 1, 2014Smolewski P, Witkowska M, Zwolinska M, Cebula-Obrzut B, Majchrzak A, Jeske A, Darzynkiewicz Z, Ardelt W, Ardelt B, Robak T.

(19) Admixture fine-mapping in African Americans implicates XAF1 as a possible sarcoidosis risk genePloS oneJanuary 1, 2014Levin AM, Iannuzzi MC, Montgomery CG, Trudeau S, Datta I, Adrianto I, Chitale DA, McKeigue P, Rybicki BA.

(20) Immunohistochemical localization of X-linked inhibitor of apoptosis protein in brainstem-type and cortical Lewy bodiesNeuroreportFebruary 15, 2012Kawamoto Y, Ito H, Ihara M, Takahashi R.immunohistochemical localization of XIAP in normal and diseased brains, we used a goat polyclonal anti-XIAP antibody (AF8221, R&D systems, Minneapolis, MN, USA). The deparaffinized sections were pretreated with 0.3% hydrogen

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XIAP Antibody

XIAP Antibody

XIAP Antibody

XIAP Antibody

XIAP Antibody

XIAP Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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XIAP Antibody from R&D Systems, a Bio-Techne Brand

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