Gas6 Antibody AF885 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemGas6 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF885
NCBI Gene AliasesAXLLG, AXSF
ApplicationsBinding Assay,ELISA (detection),ELISA Development,ELISA,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Dot Blot,Neutralization,Immunocytochemistry
TargetGas6
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
HostGoat
Conjugate/TagUnconjugated
ReactivityHuman,Mouse
Quantity100 ug (also 25 ug)
NCBI Full Gene Namegrowth arrest specific 6
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant human Gas6, Asp118-Ala678, Accession # NP_000811
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human Gas6 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse Gas6 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this Gas6 antibody be used for immunohistochemistry in cancer research?

Yes, the Gas6 antibody has been successfully utilized in immunohistochemistry applications, as demonstrated in studies involving various cancer types, including leiomyosarcoma and pancreatic cancer, where it was used to evaluate Gas6 expression in tumor samples (, ).

What is the significance of Gas6 expression in cancer, particularly in relation to AXL signaling?

Gas6 plays a crucial role in cancer progression by activating the AXL receptor tyrosine kinase, which is associated with increased cell migration and poor prognosis in several malignancies, including pancreatic and colorectal cancers. Studies have shown that targeting Gas6 can inhibit tumor cell plasticity and metastasis (, ).

Is there any evidence supporting the use of this antibody in studies related to chemotherapy resistance?

Yes, the Gas6 antibody has been used in research examining its role in chemotherapy resistance, particularly in prostate cancer, where Gas6 was found to promote cell survival during chemotherapy by regulating cell cycle progression and inhibiting apoptosis ().

What species does this antibody react with, and how does that affect its application in research?

The Gas6 antibody is reactive with human (Homo sapiens) and mouse (Mus musculus) proteins, allowing its application in comparative studies between these species, which is beneficial for translational research and understanding the role of Gas6 in various biological contexts ().

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Establishing Tyro3, Axl, and Mertk Chinese hamster ovary (CHO) reporter cell lines for cancer immunology and therapeutic applicationsMethods in Cell BiologyJuly 19, 2025Ahmed Aquib, Ziren Wang, Varsha Gadiyar, Rachael Pulica, Christopher Varsanyi, Trevor Frederick, Wen-I. Tsou, Stanley G. Kimani, Sergey Smirnov, Mariana S. De Lorenzo, et al.pSecTaq-hGas6pSecTaq-hPros1pEF2-FL-hMer_γR1pEF2-FL-hAxl_γR1pEF2-FL-hTyro3_γR1pEF2-EGFR-hMerpEF2-EGFR-hAxlpEF2-EFFR-hTyro3Antibodies: anti-β-Actin (MAB374, Cell Signaling) (see Notes 1)anti-human Gas6 Antibody (AF885, R&D systems) (see Notes 1)Human Protein S antibody (MAB4036, R&D systems) (see Notes 1)Anti-human Gla

(2) Distinct tumor architectures and microenvironments for the initiation of breast cancer metastasis in the brainCancer cellOctober 14, 2024Gan S, Macalinao DG, Shahoei SH, Tian L, Jin X, Basnet H, Bibby C, Muller JT, Atri P, Seffar E, et al.anti-mGas6 antibody,R&D Systems,Cat #: AF986-SPRRID:AB_3076301 Goat anti-hGas6 antibody,R&D Systems,Cat #: AF885-SPRRID:AB_3076302 Rabbit anti-Tenascin C antibody,Millipore,Cat #: AB19011RRID:AB_2203804 Rabbit anti-Tenascin

(3) Phase 1b study of batiraxcept in combination with durvalumab in patients with platinum-resistant ovarian canceriScienceMay 17, 2024Anne Knisely, Emily M. Hinchcliff, Elisabeth Gardiner, Reshma Rangwala, Kathryn Lito, Bryan Fellman, Ying Yuan, Anil K. Sood, Shannon N. Westin, Karen H. Lu, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Recombinant Anti-PD-L1 Antibody,Abcam,Cat#205921; RRID: AB_2687878 Human Gas6 Antibody,R&D Systems,Cat#AF885; RRID: AB_2108079 GAS6 Rabbit anti-Human Polyclonal Antibody,LSBio,Cat#LS-B13094; RRID: AB_3096951 Human

(4) Alcohol and HIV-Derived Hepatocyte Apoptotic Bodies Induce Hepatic Stellate Cell ActivationBiologyJuly 14, 2022New-Aaron M, Dagur RS, Koganti SS, Ganesan M, Wang W, Makarov E, Ogunnaike M, Kharbanda KK, Poluektova LY, Osna NA.

(5) Growth Arrest-Specific 6 in Chromophobe Renal Cell CarcinomaOncologyJanuary 1, 2022Mikuteit M, Zschäbitz S, Erlmeier M, Autenrieth M, Weichert W, Hartmann A, Steffens S, Erlmeier F.immunohistochemistry (IHC). Therefore, 2 mm TMA slides were stained for Gas6 (Anti-Gas6 antibody, R&D Systems, AF885, dilution 1:20) with a fully automated Dako Autostainer (Dako, Agilent pathology systems). Antigen retrieval

(6) Inhibition of AXL and VEGF-A Has Improved Therapeutic Efficacy in Uterine Serous CancerCancersNovember 23, 2021Toboni MD, Lomonosova E, Bruce SF, Tankou JI, Mullen MM, Schab A, Oplt A, Noia H, Wilke D, Kuroki LM, et al.(1:1000, #9272), and phospho-AKT Ser473 (1:500, #9271) from Cell Signaling Technology and GAS6 (1:1000, #AF885) from R&D Systems and corresponding secondary peroxidase-conjugated anti-rabbit IgG (H + L) (Jackson ImmunoResearch

(7) Autocrine signaling by receptor tyrosine kinases in urothelial carcinoma of the bladderPloS oneJanuary 1, 2021Lee YH, Lee MM, De Silva DM, Roy A, Wright CE, Wong TK, Costello R, Olaku O, Grubb RL 3rd, Agarwal PK, et al.(cat. # BAF358, AF276), RYK (cat. #AF4907), CSF1 (cat. #AF216), CSF1R (cat. #MAB3291) and GAS6 (cat. # AF885) were obtained from R&D Systems (Minneapolis, Minnesota USA). Cabozantinib and TP0903 were obtained

(8) Blockade of Stromal Gas6 Alters Cancer Cell Plasticity, Activates NK Cells, and Inhibits Pancreatic Cancer MetastasisFrontiers in immunologyJanuary 1, 2020Ireland L, Luckett T, Schmid MC, Mielgo A.concentrated using StrataClean Resin (Agilent Technologies) and immunoblotted for Gas6 (R&D Systems, AF885). Immunoblotting FC1242 cells were plated in DMEM media with 10% FBS for 24 h, harvested and lysed

(9) Role of Gas6 and TAM Receptors in the Identification of Cardiopulmonary Involvement in Systemic Sclerosis and Scleroderma Spectrum DisordersDisease markersJanuary 1, 2020Bellan M, Dimagli A, Piccinino C, Giubertoni A, Ianniello A, Grimoldi F, Sguazzotti M, Nerviani A, Barini M, Carriero A, et al.

(10) Proseek single-plex protein assay kit system to detect sAxl and Gas6 in serological material of brain tumor patientsBiotechnology reports (Amsterdam, Netherlands)June 1, 2018Jaksch-Bogensperger H, Hammerschmid A, Aigner L, Trinka E, Gehwolf R, Ebner Y, Hutterer M, Couillard-Despres S.in ELISAs, but not for the Proseek reagent Kit system, to detect sAxl (AF154, R&D systems) and Gas6 (AF885, R&D systems). The Proseek Assay Development kit has been performed as described in the user manual

(11) Inhibition of type I interferon responses by adenovirus serotype-dependent Gas6 bindingVirologyFebruary 1, 2018Nidetz NF, Gallagher TM, Wiethoff CM.fractions were collected and assessed for the presence of Gas6 with goat anti-Gas6 antibody (R&D systems, AF885) and donkey anti-goat HRP (R&D systems, HAF109). Pre-spin lanes represent 1:10 of the inoculum ran through

(12) Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathologyNature communicationsJanuary 30, 2018Fujita K, Chen X, Homma H, Tagawa K, Amano M, Saito A, Imoto S, Akatsu H, Hashizume Y, Kaibuchi K, et al.

(13) Expression and role of TYRO3 and AXL as potential therapeutical targets in leiomyosarcomaBritish journal of cancerDecember 5, 2017Dantas-Barbosa C, Lesluyes T, Loarer FL, Chibon F, Treilleux I, Coindre JM, Meeus P, Brahmi M, Bally O, Ray-Coquard I, et al.antiTYRO3 mouse monoclonal (MAB859); anti-AXL goat polyclonal (AF154); and anti-GAS6 goat polyclonal (AF885), all from R&D Systems. Following the appropriate detection kit: OmniMap antimouse HRP or OmniMap anti-goat

(14) Sympathetic Signaling Reactivates Quiescent Disseminated Prostate Cancer Cells in the Bone MarrowMolecular cancer research : MCRDecember 1, 2017Decker AM, Jung Y, Cackowski FC, Yumoto K, Wang J, Taichman RS.reagents of Zenon Alexa Fluor 488 (green) or 555 (red) labeling kit (Invitrogen). GAS6 antibody (cat. AF885, R&D System), HLA (BioLegend), and Ki67 (BioLegend) were used as primary antibodies. After washing with

(15) AXL-GAS6 expression can predict for adverse prognosis in non-small cell lung cancer with brain metastasesJournal of cancer research and clinical oncologyOctober 1, 2017Wu X, Ma W, Zhou Q, Yan H, Lim ZF, Huang M, Deng C, Yu X, Su H, Komo S, et al.

(16) Gas6 derived from cancer-associated fibroblasts promotes migration of Axl-expressing lung cancer cells during chemotherapyScientific reportsSeptember 6, 2017Kanzaki R, Naito H, Kise K, Takara K, Eino D, Minami M, Shintani Y, Funaki S, Kawamura T, Kimura T, et al.(AF154, R&D Systems), rabbit anti-phospho Axl antibody (AF2228, R&D Systems), goat anti-Gas6 antibody (AF885, R&D Systems) or mouse anti-GAPDH antibody (Chemicon, Temecula, CA, USA) according to the manufacturers’

(17) Growth Arrest-Specific 6 (GAS6) Promotes Prostate Cancer Survival by G(1) Arrest/S Phase Delay and Inhibition of Apoptosis During Chemotherapy in Bone MarrowJournal of cellular biochemistryDecember 1, 2016Lee E, Decker AM, Cackowski FC, Kana LA, Yumoto K, Jung Y, Wang J, Buttitta L, Morgan TM, Taichman RS.combined with reagents of Zenon Alexa Fluor 488 (green) or 555 (red) labeling kit (Invitrogen). GAS6 (cat. AF885, R&D System) antibody was used as primary antibody. After washing with PBS, the slides were mounted

(18) AXL is an oncotarget in human colorectal cancerOncotargetSeptember 15, 2015Martinelli E, Martini G, Cardone C, Troiani T, Liguori G, Vitagliano D, Napolitano S, Morgillo F, Rinaldi B, Melillo RM, et al.The primary antibodies were incubated overnight at 4°C at the concentration of 10ug/mL for anti-GAS6 (AF885, Goat mAb, R&D Systems) and 1:300 for anti-AXL (C89E7, Rabbit mAb, Cell Signaling), washed and then

(19) Growth Arrest-Specific 6 Protein in Patients with Sjögren Syndrome: Determination of the Plasma Level and Expression in the Labial Salivary GlandPloS oneJanuary 1, 2015Chen CH, Chen HC, Chang CC, Peng YJ, Lee CH, Shieh YS, Hung YJ, Lin YF.between steps. Specimens of the LSG were stained with goat antibody polyclonal to Gas6 (R&D Systems, #AF885) that was diluted in Dako diluent (Dako Denmark A/S, Glostrup, Denmark, #s3022) for 1 h at room temperature,

(20) Higher expression of receptor tyrosine kinase Axl, and differential expression of its ligand, Gas6, predict poor survival in lung adenocarcinoma patientsAnnals of surgical oncologyDecember 1, 2013Ishikawa M, Sonobe M, Nakayama E, Kobayashi M, Kikuchi R, Kitamura J, Imamura N, Date H.(sc-20741, 1:100, Santa Cruz Biotechnology, Inc., CA, USA) or a goat anti-human polyclonal Gas6 antibody (AF885, 1:100, R&D Systems Inc., MN, USA). The slides were then incubated for 50 min each with the secondary

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Gas6 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

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Gas6 Antibody from R&D Systems, a Bio-Techne Brand

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