IGF-I/IGF-1 Antibody AF791 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemIGF-I/IGF-1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF791
ApplicationsImmunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,In vivo assay,Neutralization,Immunocytochemistry
TargetIGF-I/IGF-1
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
Quantity100 ug (also 25 ug)
ReactivityHuman,Mouse,Rat,Transgenic Mouse
HostGoat
ClonalityPolyclonal
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Gene AliasesIgf-1, Igf-I, C730016P09Rik, IGF, IGFI, IGF-I, MGF
NCBI Full Gene Nameinsulin like growth factor 1, insulin-like growth factor 1
ImmunogenE. coli-derived recombinant mouse IGF-I/IGF-1, Gl33-Ala102, Accession # Q8CAR0

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse IGF-I/IGF-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human IGF-I/IGF-1 is observed, and less than 2% cross-reactivity with recombinant mouse IGF-II/IGF-2 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study the role of IGF-1 in spinal cord injury models?

Yes, the IGF-I/IGF-1 Antibody has been utilized in studies examining the role of IGF-1 in spinal cord injury. For instance, microglia-derived factors, including IGF-1, were shown to be crucial for glial scar formation and neuronal survival after spinal cord injury, as reported in the study by Bellver-Landete et al. (2019) .

Is this antibody effective for detecting IGF-1 in immunofluorescence applications?

The IGF-I/IGF-1 Antibody has been shown to work effectively in immunofluorescence applications. It was used in a study to detect IGF-1 in microglia, highlighting its utility in visualizing the expression of this growth factor in various cellular contexts .

What species can this antibody detect, and how specific is it?

This antibody detects IGF-1 in mouse, human, and rat samples. It exhibits approximately 15% cross-reactivity with recombinant human IGF-I/IGF-1 and less than 2% cross-reactivity with recombinant mouse IGF-II/IGF-2, indicating a high level of specificity for mouse IGF-1 .

Has this antibody been cited in research related to cancer studies?

Yes, the IGF-I/IGF-1 Antibody has been cited in cancer research, particularly in studies investigating the role of IGF-1 in promoting tumor growth and invasion. For example, Hillers et al. (2018) demonstrated that adipose-derived stromal cells from obese mice increased IGF-1 expression, which contributed to breast cancer cell invasion .

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Astrocyte-microglia crosstalk via CSF1 and IFN-β promotes central nervous system repairCell ReportsJune 23, 2026Tuoxin Cao, Matin Hemati-Gourabi, Yongliang Liu, Anna E. Mills, Lauren Baur, Ellie P. Rice, Theo Klippel, Yiwen Yan, Xiu Xu, William K. Fenske, et al.BD Bioscience, 550539), and IFNβ (rabbit, 1:500, Novus, NBP1-77288), IGF1 (goat, 1:100, Biotechne-R&D Systems, AF791), Ki67 (mouse, 1:000, BD Bioscience, 550609), NeuN (guinea pig, 1:2000, Sigma, ABN90 or 1:500, rabbit

(2) Central Administration of Recombinant IGF1 Is Neuroprotective in a Rodent Model of Acute Liver FailureInt J Mol SciApril 16, 2026Yubo Wang, Matthew McMillin, Gabriel Frampton, Kathryn Rhodes, Elaina Williams, Juliet Venter, Jace Tyson, Esha Gupta, Mihika Patankar, Patrick Mireles, et al.MN, USA). Mouse IGF-1 and fractalkine-neutralizing antibodies were purchased from R&D Systems (Cat#AF791 and AF472, Minneapolis, MN, USA). Gene-specific primers were purchased from commercial sources: Igf1

(3) Airway immune profiles and therapeutic implications of IGF1 in eosinophilic granulomatosis with polyangiitisNature CommunicationsJanuary 7, 2026Cong Dong, Bingtai Lu, Changhao Zhong, Changxing Ou, Xiaojing Yang, Lingwei Wang, Xiaoyu Zuo, Lingna Xue, Chenyang Lu, Shang Wang, et al.stimulation, 5 μg mouse recombinant IGF1 (R&D Systems, Cat# 791-MG,) or anti-mouse IGF1 (R&D Systems, Cat# AF791) in 30 μL sterile PBS were administrated on day 0 and day 14 (1 day before the sensitization), and the

(4) Epithelial and stromal remodeling in murine Crohn's disease-like ileitisbioRxiv : the preprint server for biologyNovember 9, 2025Letai KC, Islam BN, Menghini P, De Salvo C, Khandekar NS, Armstrong BW, Tomar A, Curry K, Pizarro TT, Tesar PJ, et al.Novus Biologicals NBP1–05030), c-MAF (anti-rabbit AF647, Proteintech 55013–1-AP), IGF1 (anti-goat AF488, Biotechne AF791), COL1A1 (anti-rabbit AF594, Boster PA2140–2), ACTA2 (anti-mouse AF647, Abcam ab7817). Slides were washed

(5) 4E-BP1-dependent translation in microglia controls mechanical hypersensitivity in male and female miceThe Journal of clinical investigationJune 2, 2025Lister KC, Wong C, Cai W, Uttam S, Stecum P, Rodrigues R, Hooshmandi M, Brown N, Fan J, Francois-Saint-Cyr N, et al.

(6) Maternal behavior promotes resilience to adolescent stress in mice through a microglia-neuron axisNature communicationsMarch 8, 2025Chen H, Xu R, Wang J, Gao F, Lv Y, Li X, Li F, Zhao J, Zhang X, Wang J, et al.mPFC at a rate of 0.5 μL/min while the volume was 1.5 μL/side once a day. The IGF-1 nAb (R&D Systems, AF791) or control IgG (R&D Systems, AB-108-C) was diluted in PBS, and injected into the bilateral mPFC with

(7) Negative feedback between PTH1R and IGF1 through the Hedgehog pathway in mediating craniofacial bone remodelingJCI InsightFebruary 10, 2025Yi Fan, Ping Lyu, Jiahe Wang, Yali Wei, Zucen Li, Shiwen Zhang, Takehito Ouchi, Junjun Jing, Quan Yuan, Clifford J. Rosen, et al.Abcam, ab21286), anti-PERIOSTIN (1:200, Abcam, ab14041), anti-IGF1 (1:50, R&D Systems, Bio-Techne, AF791), anti-IGF1 (1:50, Santa Cruz Biotechnology, sc518040), anti-IGF2 (1:200, Abcam, ab9574), anti-PTCH1

(8) Choroid plexus extracellular vesicle transport of blood-borne insulin-like growth factor 1 to the hippocampus of the immature brainPNAS NexusNovember 28, 2024Niklas Ortenlöf, Suvi Vallius, Helena Karlsson, Claes Ekström, Amanda Kristiansson, Bo Holmqvist, Stanislava Pankratova, Norman Barton, David Ley, Magnus GramAbcam), IGF-1 (1:100, Rabbit antimouse, bs-0014R, Bioss, Woburn, MA, USA), IGF-1 (1:100, goat antimouse, AF791, R&D systems), IGF-1R (1:20, goat antimouse, AF-305-NA, R&D systems), ZO-1 (1:50, rabbit antimouse,

(9) Overexpression of Igf2-derived Mir483 inhibits Igf1 expression and leads to developmental growth restriction and metabolic dysfunction in miceCell reportsSeptember 24, 2024Sandovici I, Fernandez-Twinn DS, Campbell N, Cooper WN, Sekita Y, Zvetkova I, Ferland-McCollough D, Prosser HM, Oyama LM, Pantaleão LC, et al.

(10) Acinar-ductal cell rearrangement drives branching morphogenesis of the murine pancreas in an IGF/PI3K-dependent mannerDevelopmental CellFebruary 5, 2024Jean-Francois Darrigrand, Anna Salowka, Alejo Torres-Cano, Rafael Tapia-Rojo, Tong Zhu, Sergi Garcia-Manyes, Francesca M. Spagnoli133496), E-Cadherin (1:600, Merck U3254), Glucagon (1:500, Immunostar Inc. 20076), Igf1 (1:300, Biotechne AF791), Igf1r (1:200, Biotechne AF305-NA), Insulin (1:2, Dako IR002), Integrin-α6 (1:400, Millipore MAB1378),

(11) The super-healing MRL strain promotes muscle growth in muscular dystrophy through a regenerative extracellular matrixJCI insightJanuary 4, 2024O'Brien JG, Willis AB, Long AM, Kwon J, Lee G, Li FW, Page PG, Vo AH, Hadhazy M, Spencer MJ, et al.and catalog MA515065, Invitrogen), anti–mIL-6 (catalog AF-406-NA, R&D Systems), anti–mIGF-1 (catalog AF791, R&D Systems), anti-THBS4 (catalog AF2390, R&D Systems), and anti–TGF-β3 (catalog ab15537, Abcam). Secondary

(12) AAV-Mediated nuclear localized PGC1α4 delivery in muscle ameliorates sarcopenia and aging-associated metabolic dysfunctionsAging cellOctober 1, 2023Guo M, Zhang J, Ma Y, Zhu Z, Zuo H, Yao J, Wu X, Wang D, Yu J, Meng M, et al.cocultured and neutralizing antibody assays, mouse IGF1 (4 μg/mL) neutralizing antibodies (R&D Systems, AF791) or IgG (Beyotime, A7028) were added into cultured media consists of conditioned medium and differentiation

(13) Myonectin protects against skeletal muscle dysfunction in male mice through activation of AMPK/PGC1α pathwayNature communicationsAugust 4, 2023Ozaki Y, Ohashi K, Otaka N, Kawanishi H, Takikawa T, Fang L, Takahara K, Tatsumi M, Ishihama S, Takefuji M, et al.

(14) Osteoclast-derived IGF1 induces RANKL production in osteocytes and contributes to pagetic lesion formationJCI insightJuly 24, 2023Miyagawa K, Tenshin H, Mulcrone PL, Delgado-Calle J, Subler MA, Windle JJ, Chirgwin JM, Roodman GD, Kurihara N.role in PDL formation in PD. Methods Chemicals. Rabbit IgG (catalog AB-105-C), anti-IGF1 (catalog AF791), and anti-IGF1R (catalog MBA391-100) were purchased from R&D Systems. αMEM was from Thermo Fisher Scientific

(15) Bone marrow-derived IGF-1 orchestrates maintenance and regeneration of the adult skeletonProceedings of the National Academy of Sciences of the United States of AmericaJanuary 3, 2023Wang J, Zhu Q, Cao D, Peng Q, Zhang X, Li C, Zhang C, Zhou BO, Yue R.

(16) Single-cell transcriptome analyses reveal microglia types associated with proliferative retinopathyJCI insightDecember 8, 2022Liu Z, Shi H, Xu J, Yang Q, Ma Q, Mao X, Xu Z, Zhou Y, Da Q, Cai Y, et al.Thermo Scientific), rabbit Fabp5 (1:200, 2.5 μg/mL, RD181060100, BioVendo), rabbit Igf1 (10 μg/mL, AF791, R&D Systems), rat F4/80 (1:100, ab6640, Abcam), rabbit IBa1 (1:400, 019-19741, Sakura Finetek), goat

(17) Stress Granules Determine the Development of Obesity-Associated Pancreatic CancerCancer discoveryAugust 5, 2022Fonteneau G, Redding A, Hoag-Lee H, Sim ES, Heinrich S, Gaida MM, Grabocka E.processed and stained for phosphorylated S6 as described above. Mouse Igf1 antibody (R&D Systems Cat# AF791, RRID:AB_2248752) and IgG control (R&D Systems Cat# AB-108-C, RRID:AB_354267) were resuspended in PBS

(18) Resident cardiac macrophages mediate adaptive myocardial remodelingImmunitySeptember 14, 2021Wong NR, Mohan J, Kopecky BJ, Guo S, Du L, Leid J, Feng G, Lokshina I, Dmytrenko O, Luehmann H, et al.(Cell Signaling cat# 13255), Desmoplakin I/II clone DP2.15 (Abcam Cat# ab16434), IGF1 (R&D systems Cat# AF791), CYR61 (R&D systems Cat# AF4055), Ly6G clone 1A8 (BD Cat# 551459), HCN4 (Fisher Cat# PA5-111878), FAK

(19) Microglia-neuron interaction at nodes of Ranvier depends on neuronal activity through potassium release and contributes to remyelinationNature communicationsSeptember 1, 2021Ronzano R, Roux T, Thetiot M, Aigrot MS, Richard L, Lejeune FX, Mazuir E, Vallat JM, Lubetzki C, Desmazières A.

(20) Muscle insulin-like growth factor-I modulates murine craniofacial bone growthEuropean cells & materialsJuly 19, 2021Kok HJ, Crowder CN, Koo Min Chee L, Choi HY, Lin N, Barton ER.

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