Leptin/OB Antibody AF498 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemLeptin/OB Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF498
NCBI Gene AliasesLEPD, ob, OB, obese, OBS
ApplicationsELISA Development,Immunohistochemistry,Western Blot,Immunohistochemistry-Frozen,In vivo assay,Neutralization,ELISA Capture (Matched Antibody Pair),ELISA Capture
Quantity100 ug (also 25 ug)
HostGoat
ReactivityHuman,Mouse,Transgenic Mouse
Conjugate/TagUnconjugated
TargetLeptin/OB
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
NCBI Full Gene Nameleptin
ClonalityPolyclonal
ConcentrationLYOPH
Antibody TypePrimary Antibodies
StorageStore the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date
PurityAntigen Affinity-purified

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse Leptin/OB in ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) A Novel Mechanism for Depot-Specific Leptin Gene Expression Regulation and its Persistence After Weight LossiScienceApril 17, 2026Natalie Taege, Jan Hendric Britsemmer, Andreas Israel, Christin Krause, Sina Junge, Annette Feuchtinger, Siegfried Ussar, Paul Thomas Pfluger, Timo Gemoll, Sonja Charlotte Schriever, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, anti-Leptin antibody,"R&D systems, US",AF498; RRID:AB_355394 "anti-Leptin antibody, polyclonal rabbit anti-leptin antibody","Abcam, US",ab16227;

(2) Chemerin influences blood lipid of aged male mice under high fat diet and exercise states through regulating the distribution and browning of white adipose tissueCytokineSeptember 1, 2024Qu J, Fu S, Yin L, Zhang Q, Wang X.

(3) Deletion of endothelial leptin receptors in mice promotes diet-induced obesityScientific reportsMay 22, 2023Gogiraju R, Witzler C, Shahneh F, Hubert A, Renner L, Bochenek ML, Zifkos K, Becker C, Thati M, Schäfer K.(CL8942AP; Cedarlane Laboratories), anti-CD206 (ab64693; abcam), anti-VEGF (ABS82, Millipore), anti-LepR (AF498; R&D Systems), and anti-CD31 (sc18916; Santa Cruz Biotechnology). Sections were washed with PBS, followed

(4) Ectopic Leptin Production by Intraocular Pancreatic Islet Organoids Ameliorates the Metabolic Phenotype of ob/ob MiceMetabolitesJune 14, 2021Leibiger B, Moede T, Valladolid-Acebes I, Paschen M, Visa M, Leibiger IB, Berggren PO.

(5) Secreted frizzled-related protein 4 promotes brown adipocyte differentiationExperimental and therapeutic medicineJune 1, 2021Guan H, Zheng H, Zhang J, Xiang A, Li Y, Zheng H, Xu L, Liu E, Yu Q.temperature (Ab): Anti-adiponectin (cat. no. AF1119; R&D Systems, Inc.; 1:1,000), anti-leptin (cat. no. AF498; R&D; 1:1,000), anti-fatty acid-binding protein (FABP4) (cat. no. AF1443; R&D; 1:1,000), anti-UCP-1

(6) Obesity-associated leptin promotes chemoresistance in colorectal cancer through YAP-dependent AXL upregulationAmerican journal of cancer researchJanuary 1, 2021Chen YC, Chien CY, Hsu CC, Lee CH, Chou YT, Shiah SG, Liu SY, Yen CY, Hsieh AC, Wabitsch M, et al.

(7) STAT3 Activation-Induced Fatty Acid Oxidation in CD8⁺ T Effector Cells Is Critical for Obesity-Promoted Breast Tumor GrowthCell metabolismJanuary 7, 2020Zhang C, Yue C, Herrmann A, Song J, Egelston C, Wang T, Zhang Z, Li W, Lee H, Aftabizadeh M, et al.AB_476697 Leptin neutralizing antibody: Polyclonal Goat anti-mouse Leptin antibody,"R&D Systems, Inc.",Cat# AF498; RRID: AB_355394 Alexa Fluor 555 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody,Thermo Fisher

(8) Exercise reduces inflammatory cell production and cardiovascular inflammation via instruction of hematopoietic progenitor cellsNature medicineNovember 1, 2019Frodermann V, Rohde D, Courties G, Severe N, Schloss MJ, Amatullah H, McAlpine CS, Cremer S, Hoyer FF, Ji F, et al.mice were intubated and ventilated with 2% isoflurane supplemented with oxygen. Anti-leptin antibody (AF498) or IgG isotype control antibody (AB-108-C, both R&D Systems) were administered at a concentration of

(9) Oncolytic Viruses Engineered to Enforce Leptin Expression Reprogram Tumor-Infiltrating T Cell Metabolism and Promote Tumor ClearanceImmunitySeptember 17, 2019Rivadeneira DB, DePeaux K, Wang Y, Kulkarni A, Tabib T, Menk AV, Sampath P, Lafyatis R, Ferris RL, Sarkar SN, et al.reader. Antibodies used for leptin Elisa experiment: Capture Mouse Leptin/OB antibody (R&D systems AF498) and detection antibody Mouse Leptin/OB antibody (R&D system BAF498). TCR Sequencing CL24 tumors treated

(10) Elevated leptin disrupts epithelial polarity and promotes premalignant alterations in the mammary glandOncogeneMay 1, 2019Tenvooren I, Jenks MZ, Rashid H, Cook KL, Muhlemann JK, Sistrunk C, Holmes J, Wang K, Bonin K, Hodges K, et al.(MilliporeSigma, Burlington MA, USA, 07–330, 2 µg/ml) and leptin (R&D Systems Minneapolis MN, USA, AF498, 2 µg/ml) antibodies. Tissue sections were stained with hematoxylin, and a Mantra system (Perkin Elmer,

(11) Involvement of leptin signaling in the development of cannabinoid CB2 receptor-dependent mirror image painScientific reportsJuly 17, 2018Nozaki C, Nent E, Bilkei-Gorzo A, Zimmer A.

(12) JAK/STAT3-Regulated Fatty Acid β-Oxidation Is Critical for Breast Cancer Stem Cell Self-Renewal and ChemoresistanceCell metabolismJune 5, 2018Wang T, Fahrmann JF, Lee H, Li YJ, Tripathi SC, Yue C, Zhang C, Lifshitz V, Song J, Yuan Y, et al.“Leptin neutralizing antibody: Polyclonal Goat anti-mouse Leptin antibody; R&D Systems, Inc.; Cat# AF498; RRID: AB_355394.” In addition, under Experimental Model and Subject Details, MMTV-PyMT (PyMT)/B6 (Davie

(13) Leptin/Osteopontin Axis Regulated Type 2T Helper Cell Response in Allergic Rhinitis with ObesityEBioMedicineJune 1, 2018Zeng Q, Luo X, Han M, Liu W, Li H.

(14) Extra-adrenal glucocorticoids contribute to the postprandial increase of circulating leptin in miceJournal of cell communication and signalingJune 1, 2018Tomabechi Y, Tsuruta T, Saito S, Wabitsch M, Sonoyama K.anti-mouse leptin antibody, biotin-conjugated anti-mouse leptin antibody, and recombinant mouse leptin (AF498, BAF498, and 498-OB-01M, respectively, R&D Systems) were used for the capture antibody, detection antibody,

(15) Data on the expression of leptin and leptin receptor in the dorsal root ganglion and spinal cord after preganglionic cervical root avulsionData in briefDecember 1, 2017Chang KT, Lin YL, Lin CT, Tsai MJ, Huang WC, Shih YH, Lee YY, Cheng H, Huang MC.Germany)], neuronal marker [NeuN (1:100; MAB377, chemicon, Merck KGaA, Darmstadt, Germany)], leptin (1:50; AF498, R&D system, Minneapolis, MN, USA), and the LepR (1:100, AF497, R&D Systems, Minneapolis, MN, USA).

(16) Rapid linkage of innate immunological signals to adaptive immunity by the brain-fat axisNature immunologyMay 1, 2015Kim MS, Yan J, Wu W, Zhang G, Zhang Y, Cai D.

(17) Personalized integrative oncology: targeted approaches for optimal outcomes: the 11th International Conference of the Society for Integrative OncologyIntegrative cancer therapiesJanuary 1, 2015Lee RT, Yang P, Greenlee H, Bauer-Wu S, Balneaves LG, Zick S.conditioned media that had been preincubated with either a leptin neutralizing antibody (R&D Systems # AF498) or a soluble chimeric leptin receptor (R&D Systems # 497-LR-100). Tumorspheres were allowed to grow

(18) Leptin mediates the increase in blood pressure associated with obesityCellDecember 4, 2014Simonds SE, Pryor JT, Ravussin E, Greenway FL, Dileone R, Allen AM, Bassi J, Elmquist JK, Keogh JM, Henning E, et al.Neutralization of Endogenous LeptinTo neutralize leptin’s actions a goat IgG against recombinant mouse leptin (AF498 from R and D systems, Minneapolis) was peripherally (i.p.) administered into DIO mice for 5 consecutive

(19) Pluronic modified leptin with increased systemic circulation, brain uptake and efficacy for treatment of obesityJournal of controlled release : official journal of the Controlled Release SocietyOctober 10, 2014Yi X, Yuan D, Farr SA, Banks WA, Poon CD, Kabanov AV.temperature (RT). To detect the blot, the membrane was probed with anti-leptin polyclonal antibody (AF498, goat IgG anti-mouse leptin, R&D Systems, Minneapolis, MN) in TBST containing 1% BSA overnight at 4

(20) Hypothalamic tanycytes are an ERK-gated conduit for leptin into the brainCell metabolismFebruary 4, 2014Balland E, Dam J, Langlet F, Caron E, Steculorum S, Messina A, Rasika S, Falluel-Morel A, Anouar Y, Dehouck B, et al.

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Leptin/OB Antibody

Leptin/OB Antibody

Leptin/OB Antibody

Leptin/OB Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (29)

Reviews

Leptin/OB Antibody from R&D Systems, a Bio-Techne Brand

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