VEGFR2/KDR/Flk-1 Antibody AF357 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemVEGFR2/KDR/Flk-1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF357
ApplicationsFACS,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Neutralization,Immunocytochemistry,Functional Assay,ELISA Capture
TargetVEGFR2/KDR/Flk-1
IsotypeIgG
Conjugate/TagUnconjugated
HostGoat
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman,Mouse,Primate - Papio anubis (Olive Baboon),Rat,Xenograft
Quantity100 ug (also 25 ug)
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant human VEGFR2/KDR/Flk-1, Ala20-Glu764, Accession # AAC16450
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namekinase insert domain receptor
NCBI Gene AliasesCD309, FLK1, VEGFR, VEGFR2

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human VEGFR2/KDR/Flk-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse VEGFR2 is observed and less than 10% cross-reactivity with recombinant human (rh) VEGFR1 and rhVEGFR3 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used for immunocytochemistry in human cells?

Yes, the VEGFR2/KDR/Flk-1 Antibody has been shown to be effective for immunocytochemistry applications, as evidenced by its use in studies involving human retinal microvascular endothelial cells to evaluate VEGFR2 signaling ().

What species does this antibody react with?

The VEGFR2/KDR/Flk-1 Antibody is reactive with several species, including Homo sapiens (human), mouse, rat, and non-human primates, making it versatile for various experimental models.

Is there any evidence of this antibody being used in Western blotting?

Yes, the VEGFR2/KDR/Flk-1 Antibody has been utilized in Western blotting, as referenced in multiple studies, including those examining the phosphorylation of VEGFR2 in response to various stimuli ().

How does this antibody perform in terms of specificity for VEGFR2?

The antibody specifically detects human VEGFR2/KDR/Flk-1 in direct ELISAs and Western blots, with approximately 40% cross-reactivity with recombinant mouse VEGFR2 and less than 10% cross-reactivity with human VEGFR1 and VEGFR3, indicating a high level of specificity for its target.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

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(2) Cooperative ETS transcription factors are required for lymphatic endothelial cell integrity and resilienceJ Clin InvestDecember 23, 2025Myung Jin Yang, Seok Kang, Seon Pyo Hong, Hokyung Jin, Jin-Hui Yoon, Cheolhwa Jin, Chae Min Yuk, Lydia Getachew Gebeyehu, Junho Jung, Sung-Hwan Yoon, et al.(ab133485) (Abcam); mouse anti-VEGFR3/Flt4 (MAB3491); sheep anti-FoxC2 (AF5044); goat anti-VEGFR2/Flk-1 (AF357); sheep anti-CD31 (AF806); goat anti-GATA2 (AF2046) (R&D Systems); mouse anti-NFATC1 (MA3-024, Invitrogen);

(3) Activin A Prevents Hyperresponsiveness to Vascular Endothelial Growth Factor in Pathologic Blood Vessels by Perturbing the Trafficking of Activated Vascular Endothelial Growth Factor Receptor 2The American Journal of PathologySeptember 1, 2025Basma Baccouche, Maximilian McCann, Janani Rajasekar, Nguyễn Thị Thanh Thị Thanh Nhàn, Kaori Yamada, Andrius KazlauskasSigma-Aldrich (St. Louis, MO, USA). Human VE-cadherin antibody (MAB9381-SP) and VEGFR2 antibody for staining (AF357) were purchased from R&D Systems (Minneapolis, MN, USA). Human Rab11 (5589) was purchased from Cell Signaling

(4) VEGF-A, -C, -D, VEGFR1, -2, -3, PDGF-BB and FGF-2 join forces to induce vascular and lymphatic angiogenesis during bone healing of hip implantsBone reportsSeptember 1, 2025Cassuto J, Folestad A, Göthlin J, Malchau H, Kärrholm J.100-20D). VEGFR2, CA: antigen affinity-purified polyclonal goat IgG antibody (Biotechne R&D Systems, cat.no.AF357), DA: biotinylated antigen affinity-purified polyclonal goat IgG antibody (Biotechne R&D Systems, cat.no.BAF357),

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(8) Piezo1 regulates meningeal lymphatic vessel drainage and alleviates excessive CSF accumulationNature neuroscienceMay 1, 2024Choi D, Park E, Choi J, Lu R, Yu JS, Kim C, Zhao L, Yu J, Nakashima B, Lee S, et al.(Cell Signaling Technology, 32027), p-eNOS (Ser 1177; Cell Signaling Technology, 9571), VEGFR2 (R&D Systems, AF357), p-VEGFR2 (Tyr 1054/Tyr 1059; Invitrogen, 44-1047G), VEGFR3 (Santa Cruz Biotechnology, sc-321), p-VEGFR3

(9) Endomucin selectively regulates vascular endothelial growth factor receptor-2 endocytosis through its interaction with AP2Cell communication and signaling : CCSApril 11, 2024Cano I, Wild M, Gupta U, Chaudhary S, Ng YSE, Saint-Geniez M, D'Amore PA, Hu Z.internalization assay HRECs were serum starved for 2 h in EBM-2 and incubated with goat anti-VEGFR2 (1:100, AF357; R&D Systems) at 4 °C for 1 h, followed by the addition of bovine serum albumin (BSA) or VEGF (10 ng/ml)

(10) The development of early human lymphatic vessels as characterized by lymphatic endothelial markersThe EMBO journalMarch 1, 2024Yamaguchi S, Minamide N, Imai H, Ikeda T, Watanabe M, Imanaka-Yoshida K, Maruyama K.(anti-PDPN) (413151, nichirei biosciences, at its original concentration (ready to use product)), Flk1 (AF357, R&D Systems, 1:150, RRID:AB_355320), Isl1 (PA5-27789, Invitrogen, 1:200, RRID:AB_2545265), Ki67 (ab15580,

(11) The E2 ubiquitin-conjugating enzymes UBE2D1 and UBE2D2 regulate VEGFR2 dynamics and endothelial functionJournal of cell scienceMay 15, 2023Critchley WR, Smith GA, Zachary IC, Harrison MA, Ponnambalam S.were sourced as follows: goat polyclonal anti-VEGFR2 [1:100 for IF, 1:1000 for immunoblotting (IB), AF357] from R&D Systems, Minneapolis, MN, USA; mouse HRP-conjugated anti-α-tubulin (1:10,000 for IB, HRP-66031)

(12) Critical role of mitogen-inducible gene 6 in restraining endothelial cell permeability to maintain vascular homeostasisJournal of cell communication and signalingMarch 1, 2023Xing L, Huang G, Chen R, Huang L, Liu J, Ren X, Wang S, Kuang H, Kumar A, Kim JK, et al.starved for 4 h with serum-free media. Cells were incubated with an anti-VEGFR2 antibody (1:75 dilution, AF357, R&D Systems) at 4°C for 20 min with 0.5% FBS and rinsed with cold PBS. Cells were then incubated with

(13) Induced hepatic stem cells are suitable for human hepatocyte productioniScienceOctober 21, 2022Nakashima Y, Miyagi-Shiohira C, Saitoh I, Watanabe M, Matsushita M, Tsukahara M, Noguchi H.

(14) A predictive microfluidic model of human glioblastoma to assess trafficking of blood-brain barrier-penetrant nanoparticlesProceedings of the National Academy of Sciences of the United States of AmericaJune 7, 2022Straehla JP, Hajal C, Safford HC, Offeddu GS, Boehnke N, Dacoba TG, Wyckoff J, Kamm RD, Hammond PT.

(15) The endosomal RIN2/Rab5C machinery prevents VEGFR2 degradation to control gene expression and tip cell identity during angiogenesisAngiogenesisAugust 1, 2021Kempers L, Wakayama Y, van der Bijl I, Furumaya C, De Cuyper IM, Jongejan A, Kat M, van Stalborch AD, van Boxtel AL, Hubert M, et al.methods Antibodies, plasmids and other materials The following antibodies were used: anti-VEGFR2 (R&D Systems, AF357), anti-α-tubulin (Sigma-Aldrich, T6199), anti-AKT (Cell signaling technology, #9272), anti-p(S473)AKT

(16) The P-type ATPase transporter ATP7A promotes angiogenesis by limiting autophagic degradation of VEGFR2Nature communicationsMay 25, 2021Ash D, Sudhahar V, Youn SW, Okur MN, Das A, O'Bryan JP, McMenamin M, Hou Y, Kaplan JH, Fukai T, et al.

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(19) Paladin is a phosphoinositide phosphatase regulating endosomal VEGFR2 signalling and angiogenesisEMBO reportsFebruary 3, 2021Nitzsche A, Pietilä R, Love DT, Testini C, Ninchoji T, Smith RO, Ekvärn E, Larsson J, Roche FP, Egaña I, et al.

(20) Galectin-3 Enhances Vascular Endothelial Growth Factor-A Receptor 2 Activity in the Presence of Vascular Endothelial Growth FactorFrontiers in cell and developmental biologyJanuary 1, 2021Cano I, Hu Z, AbuSamra DB, Saint-Geniez M, Ng YSE, Argüeso P, D'Amore PA.(1:1,000, Millipore, #CP06-100UG), and goat anti-VEGFR2 (for immunocytochemistry, 1:100, R&D System, #AF357). Secondary antibodies used for immunoblots include goat anti-rabbit 800CW (1:20,000, LI-COR, #925-32211)

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