IGFBP-3 Antibody AF675 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemIGFBP-3 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $489.00Supplier Page View Company Product Page
Catalog NumberAF675
ApplicationsImmunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Neutralization,Immunocytochemistry
TargetIGFBP-3
IsotypeIgG
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
HostGoat
ReactivityHuman,Mouse,Primate - Chlorocebus aethiops (African Green Monkey)
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant human IGFBP-3, Gly28-Lys291, Accession # CAA46087
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Nameinsulin like growth factor binding protein 3
NCBI Gene AliasesBP-53, IBP3

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human IGFBP-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross reactivity with recombinant mouse IGFBP-3 is observed, and less than 1% cross-reactivity with recombinant human (rh) IGFBP-1, rhIGFBP-2, and rhIGFBP-4 is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) SEMA7A-mediated juxtacrine stimulation of IGFBP-3 upregulates IL-17RB at pancreatic cancer invasive frontCancer gene therapyDecember 1, 2024Chen YI, Tien SC, Ko YL, Chang CC, Hsu MF, Chien HJ, Peng HY, Jeng YM, Tien YW, Chang YT, et al.

(2) Defective Visceral Adipose Tissue Adaptation in Gestational Diabetes MellitusThe Journal of Clinical Endocrinology & MetabolismApril 19, 2024Colm J McElwain, Samprikta Manna, Andrea Musumeci, Isaac Sylvester, Chloé Rouchon, Anne Marie O'Callaghan, Mustafa Abdalla Bakhit Ebad, Fergus P McCarthy, Cathal M McCarthytemperature and incubated overnight at 4 °C with the following primary antibodies: IGFBP3 1:1000 (R&D Systems AF675, RRID:AB_2123342); IGFBP4 1:3000 (R&D Systems AF804, RRID:AB_355615); IRS1 1:1000 (Cell Signaling #3407,

(3) IGFBP3 induces PD-L1 expression to promote glioblastoma immune evasionCancer cell internationalFebruary 7, 2024Zhao L, Wang Y, Mu P, Zhang X, Qi R, Zhang Y, Zhang H, Zhu X, Dong Z, Dong Y.

(4) MicroRNA-210-3p Regulates Endometriotic Lesion Development by Targeting IGFBP3 in Baboons and Women with EndometriosisReproductive SciencesOctober 1, 2023Kentaro Kai, Niraj R. Joshi, Gregory W. Burns, Samantha M. Hrbek, Erin L. Vegter, Maria Ariadna Ochoa-Bernal, Yong Song, Genna E. Moldovan, Lorenzo F. Sempere, Eduardo H. Miyadahira, et al.Burlingame, CA, USA), sections were blocked and incubated with anti-IGFBP3 (goat polyclonal, 1:50 dilution, AF675, R&D Systems, Minneapolis, MN, USA) and anti-COL8A1 (rabbit polyclonal, 1:100 dilution, HPA053107, Sigma-Aldrich,

(5) Potent antitumor effects of the conditioned medium of bone marrow-derived mesenchymal stem cells via IGFBP-4Cancer scienceJune 1, 2023Furusaka Y, Inoue S, Mizoguchi I, Hasegawa H, Katahira Y, Watanabe A, Sakamoto E, Sekine A, Miyakawa S, Umezu T, et al.Healthcare) for 24 h at 4°C. The following primary antibodies were used: goat anti‐human IGFBP‐3 (#AF675; R&D Systems), mouse anti‐human IGFBP‐4 (clone #82314R; R&D Systems), mouse anti‐human IGFBP‐6 (clone

(6) Netazepide Inhibits Expression of Pappalysin 2 in Type 1 Gastric Neuroendocrine TumorsCellular and molecular gastroenterology and hepatologyJanuary 1, 2020Lloyd KA, Parsons BN, Burkitt MD, Moore AR, Papoutsopoulou S, Boyce M, Duckworth CA, Exarchou K, Howes N, Rainbow L, et al.

(7) ISL1 overexpression enhances the survival of transplanted human mesenchymal stem cells in a murine myocardial infarction modelStem cell research & therapyFebruary 26, 2018Xiang Q, Liao Y, Chao H, Huang W, Liu J, Chen H, Hong D, Zou Z, Xiang AP, Li W.

(8) Antitumor activity and immunogenicity of recombinant vaccinia virus expressing HPV 16 E7 protein SigE7LAMP is enhanced by high-level coexpression of IGFBP-3Cancer gene therapyMarch 1, 2014Musil J, Kutinova L, Zurkova K, Hainz P, Babiarova K, Krystofova J, Nemeckova S.we repeated the virus growth experiment in the presence of the neutralizing goat polyclonal antibody AF675 (antihuman IGFBP-3, R&D Systems). After a 2-h virus adsorption, cells were washed and fresh medium containing

(9) Amyloid beta-mediated epigenetic alteration of insulin-like growth factor binding protein 3 controls cell survival in Alzheimer's diseasePloS oneJanuary 1, 2014Sung HY, Choi EN, Lyu D, Mook-Jung I, Ahn JH.

(10) IGFBP-3 is a metastasis suppression gene in prostate cancerCancer researchAugust 1, 2011Mehta HH, Gao Q, Galet C, Paharkova V, Wan J, Said J, Sohn JJ, Lawson G, Cohen P, Cobb LJ, et al.

(11) Human IGF binding protein-3 overexpression impairs glucose regulation in mice via an inhibition of insulin secretionEndocrinologyJune 1, 2011Nguyen KH, Yao XH, Moulik S, Mishra S, Nyomba BL.

(12) Breast cancer cells induce cancer-associated fibroblasts to secrete hepatocyte growth factor to enhance breast tumorigenesisPloS oneJanuary 13, 2011Tyan SW, Kuo WH, Huang CK, Pan CC, Shew JY, Chang KJ, Lee EY, Lee WH.

(13) IGFBP3 Colocalizes with and Regulates Hypocretin (Orexin)PLoS ONEJanuary 22, 2009Makoto Honda et al.obtained. Sections were then (VIII) incubated in 1.5% rabbit serum, (IX) goat anti-IGFBP3 antiserum (1∶250; AF675 R&D Systems, Minneapolis, MN), (X) biotinylated rabbit anti-goat IgG (1∶200, Vector), (XI) ABC reagent

(14) The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pyloriGastroenterologyMay 1, 2006McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, et al.

(15) The von Hippel-Lindau tumor suppressor protein regulates gene expression and tumor growth through histone demethylase JARID1COncogeneJanuary 1, 0001"Niu X, Zhang T, Liao L, Zhou L, Lindner DJ, Zhou M, Rini B, Yan Q, Yang H."

(16) Fibrocytes boost tumor-supportive phenotypic switches in the lung cancer niche via the endothelin systemNature communicationsJanuary 1, 0001"Weigert A, Zheng X, Nenzel A, Turkowski K, Günther S, Strack E, Sirait-Fischer E, Elwakeel E, Kur IM, Nikam VS, et al."μg/mL R&D Systems #MAB293-100), IGFBP-2 (2 μg/mL R&D Systems #AF674), and IGFBP-3 (1 μg/mL R&D Systems #AF675) were depleted individually from conditioned medium of A549-fibrocyte co-culture using neutralizing

(17) The contributions of HIF-target genes to tumor growth in RCCPloS oneJanuary 1, 0001"Zhang T, Niu X, Liao L, Cho EA, Yang H."

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IGFBP-3 Antibody

IGFBP-3 Antibody

IGFBP-3 Antibody

IGFBP-3 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (17)

Reviews

IGFBP-3 Antibody from R&D Systems, a Bio-Techne Brand

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