Wnt-5a Antibody AF645 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemWnt-5a Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF645
NCBI Gene AliaseshWNT5A, 8030457G12Rik, Wnt-5a
ApplicationsBioassay,Blocking,Immunodepletion,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Microarray,Neutralization,Immunocytochemistry,ELISA Detection
IsotypeIgG
ReactivityHuman,Mouse,Rat,Frog - Xenopus (African Clawed Frog)
Quantity100 ug (also 25 ug)
HostGoat
TargetWnt-5a
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
NCBI Full Gene Namewingless-type MMTV integration site family, member 5A, Wnt family member 5A
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant mouse Wnt-5a peptide, Gln254-Cys334, Accession # P22725
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse and rat Wnt-5a in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) Wnt-5b is observed and less than 2% cross-reactivity with rmWnt-1, rmWnt-3a, rmWnt-4, rmWnt-11, and rmWnt-16 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study the role of Wnt5a in neuronal development?

Yes, the Wnt-5a antibody has been shown to promote axon outgrowth in mouse embryonic cortical neurons, indicating its relevance in studying neuronal development and polarity. Specifically, Wnt5a acts in coordination with core planar cell polarity components to facilitate axon elongation ().

Is the Wnt-5a antibody effective for detecting Wnt5a in various species?

The Wnt-5a antibody is reactive with human, mouse, rat, and Xenopus species, making it suitable for studies across these organisms. It has been utilized in various applications, including Western blotting and immunohistochemistry, to detect Wnt5a in these species (, ).

How does Wnt5a signaling relate to microglial function in the context of inflammation?

Wnt5a has been identified as a critical mediator in IL-4 induced endothelial barrier dysfunction, which is relevant in the context of inflammation. Silencing Wnt5a in human coronary artery endothelial cells significantly improved barrier function, suggesting its role in modulating microglial and endothelial interactions during inflammatory responses ().

What experimental techniques can I use this antibody with?

The Wnt-5a antibody is suitable for various applications, including Western blotting, immunocytochemistry, and immunohistochemistry. It has been effectively used in studies involving protein detection and localization in cultured cells and tissue sections (, ).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Single-cell transcriptomics reveals bladder microenvironment dynamics in Hunner type interstitial cystitisiScienceAugust 21, 2026Fumihiko Urabe, Kentaro Yoshihara, Jun Nakayama, Yuta Hirano, Naoaki Watanabe, Hironori Suzuki, Miyaka Umemori, Kagenori Ito, Taro Igarashi, Shun Sato, et al.(Cell Signaling Technology, 3174), mouse anti-Neuregulin-1 (Santa Cruz Biotechnology, E-12), and rat anti-WNT5A (R&D Systems, AF645). Quantification and statistical analysis Statistical analyses were performed using GraphPad Prism

(2) Skeletal progenitor LRP1 deficiency causes severe and persistent skeletal defects with Wnt pathway dysregulationBone ResJanuary 26, 2025Mohammad Alhashmi, Abdulrahman M. E. Gremida, Santosh K. Maharana, Marco Antonaci, Amy Kerr, Shijian Fu, Sharna Lunn, David A. Turner, Noor A. Al-Maslamani, Ke Liu, et al.followed by three times washing with PBS. Each sample was then incubated with anti-Wnt5a antibody (AF645, R&D systems) and anti-LRP1 antibody (ab92544, Abcam) for overnight at 4 °C. Cells were washed with

(3) Small Extracellular Vesicles Promote Axon Outgrowth by Engaging the Wnt-Planar Cell Polarity PathwayCellsJanuary 6, 2025Ahmad S, Christova T, Pye M, Narimatsu M, Song S, Wrana JL, Attisano L.anti-Wnt7b (1:200),"R&D Systems (Minneapolis, MN, USA)",AF3460 Goat anti-Wnt5a (1:1000),R&D Systems,AF645 Goat anti-GFP (1:1000),"Rockland Immunochemicals (Limerick, PA, USA)",600-101-215 Donkey anti-goat

(4) Wnt5a Promotes Axon Elongation in Coordination with the Wnt-Planar Cell Polarity PathwayCellsJuly 28, 2024Ahmad S, Attisano L.anti-CD81 (B-11, 1:1500)","Santa Cruz (Dallas, TX, USA)",sc-166029 Goat anti-Wnt5a (1:1000),R&D Systems,AF645 Rabbit anti-Actin (1:1000),Sigma-Aldrich,A2066 "Rabbit anti-Dvl2 (H-75, 1:1000)",Santa Cruz,sc-13974 Rabbit

(5) LGR5 Expression Predicting Poor Prognosis Is Negatively Correlated with WNT5A in Colon CancerCellsNovember 20, 2023Mehdawi LM, Ghatak S, Chakraborty P, Sjölander A, Andersson T.for the evaluation of WNT5A expression, and these samples were analyzed with the anti-WNT5A antibody (AF645, R&D Systems, Inc. Minneapolis, MN, USA). Tumor samples from seventy patients were available for the

(6) Inhibition of colony stimulating factor 1 receptor corrects maternal inflammation-induced microglial and synaptic dysfunction and behavioral abnormalitiesMolecular psychiatryJune 1, 2021Ikezu S, Yeh H, Delpech JC, Woodbury ME, Van Enoo AA, Ruan Z, Sivakumaran S, You Y, Holland C, Guillamon-Vivancos T, et al.antibody (1 μg/ml, Abcam, EPR17628), anti-WNT5A goat polyclonal antibody (0.3 μg/well, R&D Systems, AF645), and biotinylated anti-WNT5A antibody using Antibody Biotinylation Kit (1 μg/ml, Pierce). For the standard

(7) Coordinated changes in the expression of Wnt pathway genes following human and rat peripheral nerve injuryPloS oneJanuary 1, 2021van Vliet AC, Lee J, van der Poel M, Mason MRJ, Noordermeer JN, Fradkin LG, Tannemaat MR, Malessy MJA, Verhaagen J, De Winter F.for human tissue; #2530; Cell signalling, Beverly, MA, USA) goat anti-Wnt5a (1:100; for human tissue; AF645; R&D systems, Minneapolis, MN, USA), rabbit anti-Wnt5a (1:100; for rat tissue; Abcam, Cambridge, UK,

(8) Wnt5a expression and prognosis in stage II-III colon cancerTranslational oncologyJanuary 1, 2021Lund CM, Dyhl-Polk A, Nielsen DL, Riis LB.Control (NordicQC). From each tumor block 1–3 μm sections were stained with Wnt5a antibody (WNT-5a AF645 (polyclonal), R&D systems, Biotechne). The primary antibody was diluted in sterile PBS 1:100. Cytoplasmatic

(9) Salt causes aging-associated hypertension via vascular Wnt5a under Klotho deficiencyThe Journal of clinical investigationAugust 3, 2020Kawarazaki W, Mizuno R, Nishimoto M, Ayuzawa N, Hirohama D, Ueda K, Kawakami-Mori F, Oba S, Marumo T, Fujita T.(ab15251, Abcam), anti-Wnt3a (sc-136163, Santa Cruz Biotechnology), antiWnt4 (ab91226, Abcam), anti-Wnt5a (AF645, R&D Systems), antiWnt11 (ab31962, Abcam), anti-Dvl (sc-166303, Santa Cruz Biotechnology), anti-ROR2

(10) Wnt Signaling Pathway Dysregulation in the Aging Brain: Lessons From the Octodon degusFrontiers in cell and developmental biologyJanuary 1, 2020Inestrosa NC, Tapia-Rojas C, Lindsay CB, Zolezzi JM.anti-phospho-Ser33/37/Thr41β-catenin (9561) were purchased from Cell Signaling, United States. Goat anti-Wnt-5a (AF645; 1:1000) was purchased from R&D Systems United States. Mouse anti-Actin (11978) was purchased from Sigma-Aldrich,

(11) Mesenchymal WNT-5A/5B Signaling Represses Lung Alveolar Epithelial ProgenitorsCellsSeptember 25, 2019Wu X, van Dijk EM, Ng-Blichfeldt JP, Bos IST, Ciminieri C, Königshoff M, Kistemaker LEM, Gosens R.antibodies incubation (anti-vimentin, 1:100 dilution, dako M0725; WNT5A antibody, 1:50 dilution, R&D system AF645; WNT5B antibody, 1:50 dilution, Santa Cruz Sc109464) were performed in the CTBS buffer contains 1% BSA

(12) THE CANONICAL WNT PATHWAY IN GASTRIC CARCINOMAArquivos brasileiros de cirurgia digestiva : ABCD = Brazilian archives of digestive surgeryJanuary 7, 2019Oliveira LA, Oshima CTF, Soffner PA, Silva MS, Lins RR, Malinverni ACM, Waisberg J.peroxide for 20 min at room temperature. After these procedures, the sections were incubated with Wnt-5a (AF645) (1:100) and FZD5 (AF1617) purchased from R & D Systems, Inc, Minneapolis, MN, USA), 2/5 GSK3β (sc-9166)

(13) Histopathologic and immunohistochemical findings in congenital anorectal malformationsMedicineAugust 1, 2018Xiao H, Huang R, Cui DX, Xiao P, Diao M, Li L.overnight at 4°C with the appropriate dilution of primary antibodies against NSE, S-100, and C-kit (AF645; R&D Systems, China Co., Ltd). The membranes were then washed with 1XTBST and incubated with secondary

(14) Wnt5a Contributes to the Differentiation of Human Embryonic Stem Cells into Lentoid Bodies Through the Noncanonical Wnt/JNK Signaling PathwayInvestigative ophthalmology & visual scienceJuly 2, 2018Han C, Li J, Wang C, Ouyang H, Ding X, Liu Y, Chen S, Luo L.

(15) Reduced Cell Division Control Protein 42 Activity Compromises Hematopoiesis-Supportive Function of Fanconi Anemia Mesenchymal Stromal CellsStem CellsMay 1, 2018Xu J, Li X, Cole A, Sherman Z, Du W.

(16) ERAD-dependent control of the Wnt secretory factor EviThe EMBO journalFebruary 15, 2018Glaeser K, Urban M, Fenech E, Voloshanenko O, Kranz D, Lari F, Christianson JC, Boutros M.used to detect Wnt3 and Wnt3A), N‐cadherin (mouse, BD BioScience, 610921), Wnt5A (goat, R&D Systems, AF645; was used to detect Wnt5A and Wnt5B), Wnt16 (rab, GeneTex, GTX128468), Wnt11 (rab, GeneTex, GTX105971),

(17) Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the control of cell and tissue behaviors in vertebrateseLifeSeptember 8, 2017Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantú AV, Choi SS, Jong BY, Okada K, Scales MK, Hum J, et al.Cell Signaling Technology, Danvers, MA); rabbit antiEGFR (SC-03, Santa Cruz Biotechnology, Dallas, TX); goat anti-Wnt5A (AF645, R&D Systems, Minneapolis, MN); mouse anti-a-tubulin (clone DM1A, ab7291, Abcam, Cambridge, MA); chicken

(18) Wnt5a induces catabolic signaling and matrix metalloproteinase production in human articular chondrocytesOsteoarthritis and cartilageSeptember 1, 2017Huang G, Chubinskaya S, Liao W, Loeser RF.Millipore. MMP1 antibody was from Abnova. β-actin antibody was from Abcam. Wnt5a antibody (Cat. No. AF645) for immunohistochemistry (IHC), recombinant Wnt5a protein and recombinant Wnt3a protein were from R&D Systems

(19) Wnt5a is essential for hippocampal dendritic maintenance and spatial learning and memory in adult miceProceedings of the National Academy of Sciences of the United States of AmericaJanuary 24, 2017Chen CM, Orefice LL, Chiu SL, LeGates TA, Hattar S, Huganir RL, Zhao H, Xu B, Kuruvilla R.

(20) Wnt5A/Ryk signaling critically affects barrier function in human vascular endothelial cellsCell adhesion & migrationJanuary 2, 2017Skaria T, Bachli E, Schoedon G.

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Wnt-5a Antibody

Wnt-5a Antibody

Wnt-5a Antibody

Wnt-5a Antibody

Wnt-5a Antibody

Wnt-5a Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

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Wnt-5a Antibody from R&D Systems, a Bio-Techne Brand

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