Human/Mouse TIMP-2 Antibody AF971 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman/Mouse TIMP-2 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF971
NCBI Gene AliasesCSC-21K, DDC8
ApplicationsImmunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Frozen,Neutralization,Immunocytochemistry, Functional Assay,Dual RNAscope ISH-IHC Compatible
Conjugate/TagUnconjugated
IsotypeIgG
HostGoat
Quantity100 ug (also 25 ug)
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman,Mouse
TargetTIMP-2
NCBI Full Gene NameTIMP metallopeptidase inhibitor 2
ClonalityPolyclonal
ImmunogenChinese hamster ovary cell line CHO-derived recombinant human TIMP-2, Cys27-Pro220, Accession # P16035
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
ValidationKnockout Validated
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) The long noncoding RNA lnc-FANCI-2 intrinsically restricts RAS signaling in human papillomavirus type 16-infected cervical cancer cellsElifeAugust 29, 2025Haibin Liu, Lulu Yu, Vladimir Majerciak, Thomas J Meyer, Ming Yi, Peter F Johnson, Maggie Cam, Douglas R Lowy, Zhi-Ming ZhengSystems,Cat# MAB39371,Mouse monoclonal; WB (1:500) Antibody,Anti-TIMP2 (Goat polyclonal),R&D Systems,Cat# AF971,Goat polyclonal; WB (1:500) Antibody,Anti-ADAM8 (Goat polyclonal),R&D Systems,Cat# AF1031,Goat polyclonal;

(2) Youth-associated protein TIMP2 alters microglial state and function in the context of agingbioRxivJune 8, 2025Brittany M. Hemmer, Ana Catarina Ferreira, Sarah M. Philippi, Samuele F. Petridis, Annie Phan, Joseph M. Castellanofollowing concentrations: IBA1, 1:500, (019–19741, Wako); CD68, 1:200, (MCA1957, BioRad), TIMP2, 1:100 (AF971, R&D systems, [2-day incubation]), p16INK4a, 1:500, (MA5–17142, Invitrogen). Cells were then washed

(3) Elevated Serum Gastrin Is Associated with Melanoma Progression: Putative Role in Increased Migration and Invasion of Melanoma CellsInternational journal of molecular sciencesNovember 28, 2023Varga AJ, Nemeth IB, Kemeny L, Varga J, Tiszlavicz L, Kumar D, Dodd S, Simpson AWM, Buknicz T, Beynon R, et al.and MAB973, respectively), TIMP-1, TIMP-2, prosaposin, and MMP-1 (R&D Systems, Abingdon, UK; AF980, AF971, AF8520, and MAB901, respectively) were utilized. A densitometric analysis of the immunoblots was performed

(4) Fibroblasts repair blood-brain barrier damage and hemorrhagic brain injury via TIMP2Cell reportsNovember 22, 2022Xu L, Nirwane A, Xu T, Kang M, Devasani K, Yao Y.(1:1000, cell signaling, 3238S), rat anti-meca32 (1:200, Novus, NB100-77668), goat anti-TIMP2 (1:200, R&D, AF971), and mouse anti-actin (1:2000, Sigma, A5441). SuperSignal West Pico Plus Chemiluminescent Substrate

(5) Heroin Seeking and Extinction From Seeking Activate Matrix Metalloproteinases at Synapses on Distinct Subpopulations of Accumbens CellsBiological psychiatryMay 15, 2021Chioma VC, Kruyer A, Bobadilla AC, Angelis A, Ellison Z, Hodebourg R, Scofield MD, Kalivas PW.reagents Heroin HCl (NIDA), was dissolved in sterile saline. TIMP-1 and −2 antibodies (R&D Systems, #AF970 and #AF971, respectively) or normal Goat IgG (R&D Systems, #AB-108-C) were microinjected (29, 30). MMP-2i (Millipore-Sigma,

(6) Schwann Cell Stimulation of Pancreatic Cancer Cells: A Proteomic AnalysisFrontiers in oncologyJanuary 1, 2020Ferdoushi A, Li X, Griffin N, Faulkner S, Jamaluddin MFB, Gao F, Jiang CC, van Helden DF, Tanwar PS, Jobling P, et al.(AF1786) 5,Biglycan,"Goat polyclonal (1:1,000)",R&D System (AF2667) 6,TIMP-2,Goat polyclonal (1:500),R&D System (AF971) 7,Gal-1,"Goat polyclonal (1:1,000)",R&D System (AF1152) Antibodies used to detect protein in cell

(7) Co-chaperones TIMP2 and AHA1 Competitively Regulate Extracellular HSP90:Client MMP2 Activity and Matrix ProteolysisCell reportsAugust 13, 2019Baker-Williams AJ, Hashmi F, Budzyński MA, Woodford MR, Gleicher S, Himanen SV, Makedon AM, Friedman D, Cortes S, Namek S, et al.,R&D Systems,Cat# MAB002; RRID: AB_357334 "Goat IgG, isotype",Abcam,Cat# AB37373 Anti-Goat TIMP2,R&D Systems,Cat# AF971-TM; RRID: AB_355752 "Chemicals, Peptides, and Recombinant Proteins",, Ganetespib,Madrigal Pharma (formerly

(8) Bone-derived Nestin-positive mesenchymal stem cells improve cardiac function via recruiting cardiac endothelial cells after myocardial infarctionStem cell research & therapyApril 27, 2019Lu D, Liao Y, Zhu SH, Chen QC, Xie DM, Liao JJ, Feng X, Jiang MH, He W.

(9) Unique Transcriptional Programs Identify Subtypes of AKIJournal of the American Society of Nephrology : JASNJune 1, 2017Xu K, Rosenstiel P, Paragas N, Hinze C, Gao X, Huai Shen T, Werth M, Forster C, Deng R, Bruck E, et al.MAB3778; R&D), polyclonal human IGFBP7 (1:1000; AF1334; R&D Systems), and polyclonal human TIMP2 (1:1000; AF971; R&D Systems). Probe Synthesis for In Situ Hybridization Mouse kidney mRNA was reverse transcribed

(10) Human umbilical cord plasma proteins revitalize hippocampal function in aged miceNatureApril 27, 2017Castellano JM, Mosher KI, Abbey RJ, McBride AA, James ML, Berdnik D, Shen JC, Zou B, Xie XS, Tingle M, et al.euthanized. In young WT mice that had been injected with 60 μg kg−1 anti-TIMP2 antibody (R&D Systems, AF971; dose based on the in vitro ND50 (50% neutralizing dose from the manufacturer) or goat control IgG (R&D

(11) Inhibition of airway epithelial-to-mesenchymal transition and fibrosis by kaempferol in endotoxin-induced epithelial cells and ovalbumin-sensitized miceLaboratory investigation; a journal of technical methods and pathologyMarch 1, 2014Gong JH, Cho IH, Shin D, Han SY, Park SH, Kang YH.

(12) Mitral tissue inhibitor of metalloproteinase 2 is associated with mitral valve surgery outcomePloS oneJanuary 1, 2014Lin TH, Yang SF, Chiu CC, Su HM, Voon WC, Chai CY, Lai WT, Sheu SH.

(13) Timp-2 binding with cellular MT1-MMP stimulates invasion-promoting MEK/ERK signaling in cancer cellsInternational journal of cancerMarch 1, 2010Sounni NE, Rozanov DV, Remacle AG, Golubkov VS, Noel A, Strongin AY.hydroxamate inhibitor (GM6001) were from Chemicon (Temecula, CA). The goat anti-human TIMP-2 antibody (AF971) was from R&D Systems (Minneapolis, MN). The rabbit antibodies to pERK1/2 Thr202/Tyr204 (D13.14.4E),

(14) Fibroblast-conditioned media promote human sarcoma cell invasionSurgeryJanuary 1, 2009Bittner JG 4th, Wilson M, Shah MB, Albo D, Feig BW, Wang TN.

(15) Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3The Journal of cell biologyOctober 9, 2006Saunders WB, Bohnsack BL, Faske JB, Anthis NJ, Bayless KJ, Hirschi KK, Davis GE.was purchased from Becton Dickinson. Antibodies targeting MMP-10 (mAb 9101), TIMP-1 (AF970), TIMP-2 (AF971), ADAM-9 (AF949), and ADAM-15 (AF945) were obtained from R&D Systems. Antibodies targeting ADAM-12 (AB

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2/23/2022Antibody for Detection of TIMP2 Abhijit NirwaneOur lab works to understand the role of the basment membrane in physiology and disease.

Human/Mouse TIMP-2 Antibody

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Human/Mouse TIMP-2 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (15)

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Human/Mouse TIMP-2 Antibody from R&D Systems, a Bio-Techne Brand

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