CCL1/I-309/TCA-3 Antibody AF845 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCCL1/I-309/TCA-3 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $479.00Supplier Page View Company Product Page
Catalog NumberAF845
NCBI Gene AliasesTCA3, SISe, P500, Scya1, Tca3, I-309, SCYA1, Tca-3
ApplicationsFlow Cytometry,Western Blot,Neutralization
ReactivityMouse
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
HostGoat
IsotypeIgG
TargetCCL1/I-309/TCA-3
NCBI Full Gene Namechemokine (C-C motif) ligand 1, C-C motif chemokine ligand 1
ClonalityPolyclonal
ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse CCL1/I-309/TCA-3, Lys24-Cys92, Accession # P10146
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse CCL1 in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study the role of CCL1 in Treg cell regulation?

Yes, the CCL1/I-309/TCA-3 antibody has been utilized in studies examining the role of CCL1 in regulatory T cell function. For instance, research has shown that CCL1 can up-regulate CCR8 on Treg cells, enhancing their suppressive activity, which is critical in immune regulation (Barsheshet et al., 2017).

Is this antibody effective for neutralizing CCL1 in T cell activation studies?

The CCL1/I-309/TCA-3 antibody has been demonstrated to effectively neutralize CCL1 in experimental setups. In a study investigating CD8+ T cell activation, neutralization of CCL1 using this antibody resulted in reduced interaction time between T cells and dendritic cells, thereby decreasing IFN-γ production (Henry et al., 2008).

What applications is this antibody suitable for in my experiments?

This antibody is suitable for use in Western blotting, flow cytometry, and neutralization assays. It has been shown to detect mouse CCL1 in direct ELISAs and Western blots, making it versatile for various experimental approaches in cell biology.

What species does this antibody react with, and is it suitable for mouse studies?

The CCL1/I-309/TCA-3 antibody is specifically reactive with mouse CCL1, making it an appropriate choice for studies involving mouse models, particularly in immunological research focused on Treg cells and their interactions.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) CCR8⁺FOXp3⁺ T_reg cells as master drivers of immune regulationProceedings of the National Academy of Sciences of the United States of AmericaJune 6, 2017Barsheshet Y, Wildbaum G, Levy E, Vitenshtein A, Akinseye C, Griggs J, Lira SA, Karin N.Committee of the Technion. Antibodies, Cytokines, and Chemokines. Anti-mouse CCL1 neutralizing antibody (AF845), hTGF-β and hIL-2, and all recombinant chemokines (human and mouse) were purchased from R&D Systems. Human

(2) Protumoral TSP50 Regulates Macrophage Activities and Polarization via Production of TNF-α and IL-1β, and Activation of the NF-κB Signaling PathwayPloS oneJanuary 1, 2015Yang C, Zhang DM, Song ZB, Hou YQ, Bao YL, Sun LG, Yu CL, Li YX.anti-Phosphors-p65-Ser536 (3033) were obtained from Cell Signaling (Beverly, MA, USA). Anti-CCL1 (AF272 and AF845) (1:1000), anti-CCL5 (AF278 and AF478), human recombinant TNF-α, and human recombinant IL-1β were obtained

(3) Comprehensive assessment of chemokine expression profiles by flow cytometryThe Journal of clinical investigationMarch 1, 2010Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D.

(4) IL-12 produced by dendritic cells augments CD8+ T cell activation through the production of the chemokines CCL1 and CCL17Journal of immunology (Baltimore, Md. : 1950)December 15, 2008Henry CJ, Ornelles DA, Mitchell LM, Brzoza-Lewis KL, Hiltbold EM.against the chemokines TARC/CCL17 (clone 110904; R & D Systems, Jamul CA) and TCA-3/CCL1 (Cat. No. AF845; R & D Systems, Jamul CA) were added at 10µg/ml prior to T cell addition and maintained throughout the

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