Fc gamma RII/RIII (CD32/CD16) Antibody AF1460 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemFc gamma RII/RIII (CD32/CD16) Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF1460
ApplicationsImmunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunofluorescence,Immunocytochemistry,Immunocytochemistry/ Immunofluorescence
Conjugate/TagUnconjugated
HostGoat
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman,Mouse,Rat,Transgenic Mouse
TargetFc gamma RII/RIII (CD32/CD16)
IsotypeIgG
Quantity100 ug (also 25 ug)
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse Fc gamma RIIB, Thr40-Arg217, Accession # P08101
NCBI Full Gene NameFc gamma receptor IIIa, Fc receptor, IgG, low affinity IIb
NCBI Gene AliasesFCRIIIA, CD32, Fcgr2a, IMD20, F630109E10Rik, CD16A, Fcr-3, Fcr-2, FCR-10, LyM-1, FCGR3, FCG3, Ly-17, fcRII, Lym-1, CD16-II, Ly-m20, FcGRIIIA, IGFR3, Fc[g]RII, FcgRII, FCRIII, FCGRIII, CD16, Fcgr2
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse Fc gamma RII/RIII (CD32/CD16) in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human Fc gamma RII is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) A CoQ10 analog ameliorates cognitive impairment and early brain injury after subarachnoid hemorrhage by regulating ferroptosis and neuroinflammationRedox BiologyJuly 1, 2025Junhui Chen, Zhonghua Shi, Yuhua Chen, Kun Xiong, Yuhai Wang, Hongqi Zhang(1:200, rabbit polyclonal, ab64693; Abcam), rabbit anti-CD16/CD32 antibodies (1:500, rabbit polyclonal, AF1460, R&D Systems), rabbit anti-ACSL4 antibodies (1:1000, A04372-2, Wuhan BOSTER Biological Technology),

(2) Transthyretin, a novel prognostic marker of POCD revealed by time-series RNA-sequencing analysisMolecular psychiatryJuly 1, 2025Liang X, Song C, Lin J, Li S, Li L, Dai G, Zhang R, Zou OM, Yao H, Zhou L, et al.

(3) A perfusion-independent high-throughput method to isolate liver sinusoidal endothelial cellsCommun BiolJanuary 8, 2025Anna Babin-Ebell Gonçalves, Yifang Mao, Tinja Baljkas, Felix Wiedmann, Larissa Eis, Franziska Pilz, Manuel Winkler, Sina W. Kürschner-Zacharias, Marlene Hoffarth, Charlotta Funaya, et al.combinations of goat anti-mouse CD31 [R&D Systems, Cat #AF3628], goat anti-mouse CD32b [R&D Systems, Cat #AF1460], rabbit anti-mouse Desmin [Abcam, Cat # ab15200], rabbit anti-mouse ERG [Abcam, Cat #ab196149], rat

(4) Transcutaneous auricular vagus nerve stimulation improves social deficits through the inhibition of IL-17a signaling in a mouse model of autismFrontiers in PsychiatryJune 27, 2024Wenjing Zhang, Zhiwei Mou, Qi Zhong, Xiaocao Liu, Lan Yan, Lei Gou, Zhuoming Chen, Kwok-Fai So, Li Zhangfor 2 hours, primary antibodies against Iba-1 (1:300; Wako, 019–19741) or CD16 (1:300; R&D Systems, AF1460) were used for staining overnight. After rinsing, the sections were incubated with Alexa Fluor 488 (1:500;

(5) Deficiency of Stabilin-1 in the Context of Hepatic Melanoma MetastasisCancersJanuary 19, 2024Wohlfeil SA, Olsavszky A, Irkens AL, Häfele V, Dietsch B, Straub N, Goerdt S, Géraud C.Antibodies Primary antibodies: rat anti-CD31 (DM3614P, Dianova, Hamburg, Germany), goat anti-CD32b (AF1460, R&D Systems, Minneapolis, MN, USA), goat anti-Lama4 (AF3837, R&D Systems, Minneapolis, MN, USA), rabbit

(6) A cell-penetrating peptide exerts therapeutic effects against ischemic stroke by mediating the lysosomal degradation of sirtuin 5MedComm (2020)December 13, 2023Qian Xia, Xue Zhang, Gaofeng Zhan, Lu Zheng, Meng Mao, Yin Zhao, Yilin Zhao, Xing Li(18985‐1‐AP, 1:500) antibodies were obtained from Proteintech Group (Wuhan, China); an anti‐CD16/32 antibody (AF1460, 1:500) was bought from R&D Systems (Minneapolis, MN, USA); anti‐Na+‐K+/ATPase (#9339, 1:1000), anti‐Histone

(7) Association of Differentially Altered Liver Fibrosis with Deposition of TGFBi in Stabilin-Deficient MiceInternational journal of molecular sciencesJune 30, 2023Krzistetzko J, Géraud C, Dormann C, Riedel A, Leibing T.Target,"Vendor, Order Number",Application ICAM-1,"Proteintech, 10020-1-AP","IHC, IF" CD32b,"BioTechne, AF1460","IHC, IF" Lyve-1,"ReliaTech, 103-PA50","IHC, IF" Emcn,"ThermoFisher, 14-5851-85","IHC, IF" TGFBi,"Abcam,

(8) SENP3-mediated deSUMOylation of c-Jun facilitates microglia-induced neuroinflammation after cerebral ischemia and reperfusion injuryiScienceJune 16, 2023Xia Q, Mao M, Zhan G, Luo Z, Zhao Y, Li X.RRID:AB_627673 Rabbit anti-iNOS,Proteintech,Cat#18985-1-AP; RRID:AB_2782960 Mouse anti-CD16/32,R&D systems,Cat#AF1460; RRID:AB_354811 Mouse anti-β-actin,Santa Cruz Biotechnology,Cat#sc-47778; RRID:AB_626632 Rabbit anti-NF-κB p65

(9) Hepatic passaging of NRAS-mutant melanoma influences adhesive properties and metastatic patternBMC cancerMay 13, 2023Dietsch B, Weller C, Sticht C, de la Torre C, Kramer M, Goerdt S, Géraud C, Wohlfeil SA.(ab16667, Abcam, Cambridge, UK), rat anti-CD31 (DM3614P, Dianova, Hamburg, Germany), goat anti-CD32b (AF1460, R&D Systems, Minneapolis, USA), goat anti-Lyve1 (AF2125, R&D Systems, Minneapolis, USA), rat anti-Endomucin

(10) Liver sinusoidal endothelial cells show reduced scavenger function and downregulation of Fc gamma receptor IIb, yet maintain a preserved fenestration in the Glmpgt/gt mouse model of slowly progressing liver fibrosisPloS oneJanuary 1, 2023Antwi MB, Dumitriu G, Simón-Santamaria J, Romano JS, Li R, Smedsrød B, Vik A, Eskild W, Sørensen KK.temperature, and incubated overnight at 4°C with goat anti-mouse FcγRII/RIII (CD32/CD16, R&D Systems, Cat. No AF1460; working concentration: 4 μg/ml), goat anti-human MMR/CD206 (R&D Systems, Cat. No AF2534; 5 μg/ml),

(11) Motor neuron survival is associated with reduced neuroinflammation and increased autophagy after brachial plexus avulsion injury in aldose reductase-deficient miceJournal of neuroinflammationNovember 9, 2022Zhong K, Huang Y, Zilundu PLM, Wang Y, Zhou Y, Yu G, Fu R, Chung SK, Tang Y, Cheng X, et al.

(12) SENP6 induces microglial polarization and neuroinflammation through de-SUMOylation of Annexin-A1 after cerebral ischaemia-reperfusion injuryCell & bioscienceJuly 22, 2022Mao M, Xia Q, Zhan GF, Chu QJ, Li X, Lian HK.USA); Anti-iNOS (18,985–1-AP, 1:500) was purchased from Proteintech Group (Wuhan, China); anti-CD16/32 (AF1460, 1:500) was purchased from R&D systems (Minneapolis, MN, USA); anti-NF-κB p65 (#8242, 1:1000), anti-IKKα

(13) Angiogenic and molecular diversity determine hepatic melanoma metastasis and response to anti-angiogenic treatmentJournal of translational medicineFebruary 2, 2022Wohlfeil SA, Häfele V, Dietsch B, Weller C, Sticht C, Jauch AS, Winkler M, Schmid CD, Irkens AL, Olsavszky A, et al.rabbit anti-Ki-67 (ab16667, Abcam, UK), rat anti-CD31 (DM3614P, Dianova, Germany), goat anti-CD32b (AF1460, R&D Systems, USA), rabbit anti-Stabilin-2 peptide 15 antibody [33], goat anti-Lyve1 (AF2125, R&D Systems,

(14) Bone marrow sinusoidal endothelium controls terminal erythroid differentiation and reticulocyte maturationNature communicationsNovember 29, 2021Heil J, Olsavszky V, Busch K, Klapproth K, de la Torre C, Sticht C, Sandorski K, Hoffmann J, Schönhaber H, Zierow J, et al.chicken anti-GFP/YFP (Cat. No. ab13970, abcam; dilution 1:800), polyclonal goat anti-CD32 (Cat. No. AF1460, R&D Systems; dilution 1:75), polyclonal rabbit anti-alpha smooth muscle Actin (Cat. no. ab5694, abcam;

(15) Adenosine A_2A Receptor in Bone Marrow-Derived Cells Mediated Macrophages M2 Polarization via PPARγ-P65 Pathway in Chronic Hypoperfusion SituationFrontiers in aging neuroscienceJanuary 1, 2021Mou KJ, Shen KF, Li YL, Wu ZF, Duan W.NOVUS Biologicals, Building IV Centennail, CO 80112,USA), goat anti-CD16/32 antibody (M1 polarization marker, 1:200, AF1460, R&D Systems, Minneapolis, Toll Free USA, Canada), rabbit anti-Arginase-1 (Arg-1) antibody (M2 polarization

(16) Tanshinone IIA Protects Against Cerebral Ischemia Reperfusion Injury by Regulating Microglial Activation and Polarization via NF-κB PathwayFrontiers in pharmacologyJanuary 1, 2021Song Z, Feng J, Zhang Q, Deng S, Yu D, Zhang Y, Li T.p-iκB (ab133462) and p-NF-κB (p-p65) (ab239882) were purchased from Abcam (United States); CD16/32 (AF1460) and CD206 (AF2535) were purchased from R&D Systems (United States). Secondary antibodies: Alexa Fluor®

(17) Imbalanced Activation of Wnt-/β-Catenin-Signaling in Liver Endothelium Alters Normal Sinusoidal DifferentiationFrontiers in physiologyJanuary 1, 2021Koch PS, Sandorski K, Heil J, Schmid CD, Kürschner SW, Hoffmann J, Winkler M, Staniczek T, de la Torre C, Sticht C, et al.OR, United States), rat anti-CD31 (102502, BioLegend), SMA-antibody (ab5694, Abcam), goat anti-CD32b (AF1460, R&D Systems), rabbit anti-Collagen type I (R1038, Acris, Hiddenhausen, North Rhine-Westphalia, Germany),

(18) TREM2 ameliorates neuroinflammatory response and cognitive impairment via PI3K/AKT/FoxO3a signaling pathway in Alzheimer's disease miceAgingOctober 16, 2020Wang Y, Lin Y, Wang L, Zhan H, Luo X, Zeng Y, Wu W, Zhang X, Wang F.

(19) FGF21 alleviates neuroinflammation following ischemic stroke by modulating the temporal and spatial dynamics of microglia/macrophagesJournal of neuroinflammationAugust 31, 2020Wang D, Liu F, Zhu L, Lin P, Han F, Wang X, Tan X, Lin L, Xiong Y.purchased from eBioscience (San Diego, CA, USA). Antibodies applied in immunofluorescence including CD16/32(AF1460) and CD206 (AF2535) were purchased from R&D Systems (Minneapolis, MN, USA) and Iba1 (019-19741) purchased

(20) Role of IL-4 in bone marrow driven dysregulated angiogenesis and age-related macular degenerationeLifeMay 5, 2020Baba T, Miyazaki D, Inata K, Uotani R, Miyake H, Sasaki SI, Shimizu Y, Inoue Y, Nakamura K.

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Mouse Fc gamma RII/RIII (CD32/CD16) Antibody

Fc gamma RII/RIII (CD32/CD16) Antibody

Fc gamma RII/RIII (CD32/CD16) Antibody

Fc gamma RII/RIII (CD32/CD16) Antibody

Fc gamma RII/RIII (CD32/CD16) Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (26)

Reviews

Fc gamma RII/RIII (CD32/CD16) Antibody from R&D Systems, a Bio-Techne Brand

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