Axl Antibody AF154 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemAxl Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF154
ApplicationsBioassay,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,In vivo assay,Simple Western,Blockade of Receptor-ligand Interaction,Neutralization,Immunocytochemistry,CyTOF-ready, Cell Culture,Immunocytochemistry/ Immunofluorescence,ELISA Capture
ReactivityBovine,Human,Mouse,Primate,Xenograft
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
HostGoat
IsotypeIgG
TargetAxl
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant human Axl, Glu33-Pro440, Accession # AAA61243
ConcentrationLYOPH
PurityAntigen Affinity-purified
ValidationKnockout Validated
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene NameAXL receptor tyrosine kinase
NCBI Gene AliasesARK, JTK11, Tyro7, UFO

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human Axl in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant mouse Axl is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

What applications has the Axl antibody been used for in scientific research?

The Axl antibody has been shown to work in various applications including Western blotting, flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry, immunoprecipitation, and functional assays. It has been utilized in studies investigating the role of AXL in cancer signaling pathways and cellular responses.

Is the Axl antibody effective for detecting AXL in different species?

Yes, the Axl antibody is reactive with human, mouse, bovine, and non-human primate AXL proteins. It has been specifically noted for its ability to detect human AXL in direct ELISAs and Western blots, with less than 25% cross-reactivity observed with recombinant mouse AXL.

How has the Axl antibody contributed to understanding cancer mechanisms?

The Axl antibody has been used in studies that explore the role of AXL in cancer progression and drug resistance. For instance, it has been implicated in the resistance of neuroblastoma cells to ALK inhibitors, where AXL activation was associated with epithelial-to-mesenchymal transition and enhanced survival signaling.

Are there specific studies that highlight the utility of the Axl antibody in cancer research?

Yes, the Axl antibody has been cited in multiple studies, including those examining its role in non-small cell lung cancer, where it was used to assess AXL phosphorylation and its downstream signaling pathways. Additionally, it has been involved in research on breast cancer metastasis, demonstrating the potential of AXL as a therapeutic target.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) AXL is a novel ERK5/KLF4 target in MEK inhibitor-treated melanomaNeoplasiaJune 1, 2026Rupesh Paudel, Simon Goller, Stefanie Schwarz, Katharina Meder, Matthias Goebeler, Marc Schmidtdetached by brief incubation in accutase and stained with a polyclonal anti-AXL antibody (R&D System, AF154) according to manufacturer’s protocol. Staining with a suitable control antiserum served as background

(2) TYRO3, AXL, MERTK and Their Ligands in Brain Metastases From Colorectal CancersCancer MedApril 27, 2026Anaïs Noblanc, Fatima Dkhissi, Pierre-Olivier Guichet, Lucie Lebeau, Serge Milin, Sheik Emambux, Pauline Roussille, Camille Evrard, Lucie Karayan-Tapon, David Tougeron, et al.TYRO3 (rabbit polyclonal; #NBP2‐23725; Novus, Bio‐Techne; dilution 1:2000), AXL (goat polyclonal; #AF154; R&D Systems, Bio‐Techne; 1:400), MERTK (rabbit polyclonal; #MKT‐121AP; FabGennix; 1:800), PROS1 (rabbit

(3) Human brain and organoid transcriptomes reveal key receptor tyrosine kinase pathways and genetic signatures in Alzheimer's diseaseExperimental & Molecular MedicineApril 15, 2026Saewoon Shin, Xiaohui Zhu, Sarnai Amartumur, Taehoon Lee, Won Jong Yu, Soomin Park, Niloofar Etemadi, Ariunzaya Jamsranjav, Rian Kang, Gyusoo Bak, et al.5%) for 1 h in ice. The primary antibodies used for this experiment included the human AXL antibody (AF154, Bio-techne, diluted 1:1500 in Tris-buffered saline with 0.1% Tween 20 (TBST)), anti-integrin beta 1

(4) Human keratinocytes exhibit limited potential for SARS-CoV-2 infection despite ACE2 and mature cathepsin L expressionJID InnovationsMarch 1, 2026Leslie Hertereau, Manon Barthe, Noura Lamghari, Peggy Merida, Gaelle Pommier, Elisabeth Pinel, Jitendriya Swain, Delphine Muriaux, Hanan Osman-Ponchet, Véronique M. Braud(BioLegend); anti-CTSL, clone 33/1 (eBioscience, Thermo Fisher Scientific); anti-AXL, polyclonal goat IgG AF154; and anti-DPP4, polyclonal goat IgG AF1180 (R&D Systems, Bio-Techne SAS, Noyal Châtillon, France). Antibody

(5) AXL inhibition improves the therapeutic efficacy of trastuzumab in high-risk endometrial cancerGynecologic oncologyJanuary 1, 2026Tankou J, Ruau S, Walia A, Toboni M, Noia H, Mullen M, Lu K, Mutch D, Powell MA, Petricoin EF, et al.

(6) Shedding Phosphorylated Axl Receptor in Lung Adenocarcinoma: Dual-Domain Immunohistochemistry ApproachJTO Clinical and Research ReportsOctober 1, 2025Shuji Mishima, Takashi Eguchi, Yoshinori Sato, Shunichiro Matsuoka, Yuichi Oguchi, Mari Katsuno, Daisuke Nakamura, Yukihiro Terada, Hirotaka Kumeda, Kentaro Miura, et al.Furthermore, the sections were incubated overnight at 4°C with each anti-Axl antibody, including the ECD (AF154, 1:200, R&D Systems Inc., MN) or phosphorylated ICD (pICD) (Y779, 1:40, R&D Systems Inc., MN), without

(7) SFTSV utilizes AXL/GAS6 for entry via PI3K-PLC-dependent macropinocytosis activated by AXL-kinaseJournal of VirologySeptember 23, 2025Zecheng Jin, Shuhei Taguwa, Junki Hirano, Kentaro Uemura, Chikako Ono, Akatsuki Saito, Tamaki Okabayashi, Yusuke Maeda, Taroh Kinoshita, Yoshiharu Matsuuraantibody (clone 108724, R&D Systems, Minneapolis, MN, USA) and goat polyclonal anti-human AXL antibody (AF154, R&D Systems) were used to detect cell surface AXL receptors. Mouse monoclonal anti-FLAG M2 antibody

(8) Good response with nivolumab and cabozantinib combination therapy in patients with metastatic collecting duct carcinoma with high expression of PD-L1, c-MET, and AXLCEN Case ReportsSeptember 8, 2025Tatsuya Umemoto, Masanori Hasegawa, Jun Naruse, Tatsuo Kano, Nobuyuki Nakajima, Masahiro Nitta, Yoshiaki Kawamura, Hiroshi Kajiwara, Sunao Shojistaining and PD-L1 (antibody; ab205921, Abcam), c-MET (antibody; ab51067, Abcam), and AXL (antibody; AF154, R&D Systems) expressions in biopsy specimens (original magnification × 200)

(9) Detection of Gas6/AXL complex and its expression changes in patients with ST-segment elevation myocardial infarctionFrontiers in MedicineAugust 26, 2025Zhao Yin, Xiaohua Nie, Tianlun Li, Bei Zhao, Shouli Wang, Liguo Zhang, Xuyuan Zhang, Zhaoliang Shandetermination of the sAXL-Gas6 complex, AXL and Gas6. Generally, plates were coated with αAXL-4# antibody or AF154 (DY154, commercial kit from R&D system) or Gas6 antibody (DY885B, commercial kit from R&D Systems) overnight

(10) Exploiting structural variability in the kinase back-pocket to modulate polypharmacology of TAM inhibitorsEuropean Journal of Medicinal ChemistryJune 5, 2025Megan D. Hopkins, Dehui Zhang, Zhilong Chen, Andrew L. McIver, Justus M. Huelse, Jyoti P. Mahajan, Kaikai Lyu, Xiangbo Yang, Michael A. Stashko, Brittany Smith, et al.proteins were immunoprecipitated with anti-MERTK (R&D Systems, #MAB8912) or anti-AXL (R&D Systems, #AF154) antibody and Protein G agarose beads (InVitrogen, #10–1243). Phospho-proteins were detected by western blot

(11) A single mutation in the PrM gene of Zika virus determines AXL dependency for infection of human neural cellsJournal of virologyApril 15, 2025Khasa R, Ogden SC, Wang Y, Mou Z, Metzler AD, Xie X, Dai X, Tang H.populations were screened for AXL expression via western blotting with goat anti-AXL (R&D Systems, Cat# AF154). For selected clones, genomic DNA was extracted as described in the previous section. PCR primers were

(12) AXL promotes lymphangiogenesis by amplifying VEGF-C-mediated AKT pathwayCell Mol Life SciFebruary 27, 2025Sébastien Pirson, Marine Gautier-Isola, Louis Baudin, Loïc Rouaud, Aline Vanwynsberghe, Jonathan Deroye, Sophie Bekisz, Fabrice Gucciardo, Alizée Lebeau, Florence Buntinx, et al.rotation with Dynabeads Protein G (Thermo Fisher Scientific), on which AXL antibody (R&D Systems Cat# AF154, RRID: AB_354852, 1/1000) was previously bound. A flow through fraction of non-bound protein sample

(13) Vitamin K supports TGF-β1 depended in vitro human Langerhans cell differentiation and function via AxlFrontiers in ImmunologyFebruary 18, 2025Thomas Bauer, Susanne Richter-Eder, Nighat Yasmin, Jennifer Jurkin, René Köffel, Herbert Stroblindicated. Warfarin (Sigma) was added at 50 μg/ml when indicated. Axl blocking antibody from R&D systems (AF154) was used at a concentration of 5 μg/ml when indicated. Retroviral vectors, transfection of packaging

(14) Multiple cell types support productive infection and dynamic translocation of infectious Ebola virus to the surface of human skinScience advancesJanuary 3, 2025Messingham KN, Richards PT, Fleck A, Patel RA, Djurkovic M, Elliff J, Connell S, Crowe TP, Munoz Gonzalez J, Gourronc F, et al.(R&D Systems, #AF2929), Tyro3 (R&D Systems, #AF859), MerTK (R&D Systems, #AF891), AXL (R&D Systems, #AF154), DC-SIGN (R&D Systems, #MAB1621), and LSECtin (R&D Systems, #AF2947) or a control antibody (R&D Systems,

(15) MERTK Is a Potential Therapeutic Target in Ewing SarcomaCancersAugust 12, 2024Smart SK, Yeung TY, Santos MO, McSwain LF, Wang X, Frye SV, Earp HS 3rd, DeRyckere D, Graham DK.or loading control. The following antibodies were used: MERTK (ab52968, Abcam, Cambridge, UK), AXL (AF154, R&D Systems, Minneapolis, MN, USA), TYRO3 (5585S, Cell Signaling, Danvers, MA, USA), PROS1 (A0384,

(16) Lineage-dependence of the neuroblastoma surfaceome defines tumor cell state-dependent and independent immunotherapeutic targetsbioRxiv : the preprint server for biologyJuly 2, 2024Kendsersky NM, Odrobina M, Mabe NW, Farrel A, Grossmann L, Tsang M, Groff D, Wolpaw AJ, Zammarchi F, van Berkel PH, et al.

(17) Characterising plasmacytoid and myeloid AXL+ SIGLEC-6+ dendritic cell functions and their interactions with HIVPLoS pathogensJune 1, 2024Warner van Dijk FA, Tong O, O'Neil TR, Bertram KM, Hu K, Baharlou H, Vine EE, Jenns K, Gosselink MP, Toh JW, et al.

(18) Phase 1b study of batiraxcept in combination with durvalumab in patients with platinum-resistant ovarian canceriScienceMay 17, 2024Anne Knisely, Emily M. Hinchcliff, Elisabeth Gardiner, Reshma Rangwala, Kathryn Lito, Bryan Fellman, Ying Yuan, Anil K. Sood, Shannon N. Westin, Karen H. Lu, et al.anti-Human Polyclonal Antibody,LSBio,Cat#LS-B13094; RRID: AB_3096951 Human Axl Antibody,R&D Systems,Cat#AF154; RRID: AB_354852

(19) Discovery of Novel Macrocyclic MERTK/AXL Dual InhibitorsJournal of Medicinal ChemistryApril 11, 2024Deyu Kong, Qiang Tian, Zhilong Chen, Hongchao Zheng, Michael A. Stashko, Dan Yan, H. Shelton Earp, Stephen V. Frye, Deborah DeRyckere, Dmitri Kireev, et al.proteins were immunoprecipitated with anti-MERTK (R&D Systems, #MAB8912) or anti-AXL (R&D Systems, #AF154) antibody and Protein G agarose beads (InVitrogen). Phospho-proteins were detected by immunoblot using

(20) The Vitamin K-Dependent Anticoagulant Factor, Protein S, Regulates Vascular PermeabilityCurrent Issues in Molecular BiologyApril 9, 2024Aurélie Joussaume, Chryso Kanthou, Olivier E. Pardo, Lucie Karayan-Tapon, Omar Benzakour, Fatima Dkhissiantibodies,Neutralizing and Irrelevant antibodies,Neutralizing and Irrelevant antibodies AXL,Human AXL Antibody/#AF154,"R&D Systems (Minneapolis, MN, USA)",Polyclonal Goat IgG,10 µg/mL MERTK,Human MERTK Antibody/#AF891,R&D Systems

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Human Axl Antibody

Axl Antibody

Axl Antibody

Axl Antibody

Axl Antibody

Axl Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

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Axl Antibody from R&D Systems, a Bio-Techne Brand

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