ICAM-1/CD54 Antibody AF796 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemICAM-1/CD54 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF796
NCBI Gene AliasesMALA-2, CD54, P3.58, Icam-1, Ly-47, BB2
ApplicationsImmunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Adhesion Blockade,Neutralization,Immunocytochemistry
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
HostGoat
IsotypeIgG
TargetICAM-1/CD54
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHamster,Human,Mouse,Transgenic Mouse,Transgenic Rat
NCBI Full Gene Nameintercellular adhesion molecule 1
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse ICAM-1/CD54, Gln28-Asn485, Accession # Q3U8M7
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse ICAM-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant rat ICAM-1 is observed, approximately 5% cross-reactivity with recombinant human (rh) ICAM-1 and rhICAM-3 is observed, and less than 1% cross-reactivity with recombinant mouse ICAM-2 is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Plasmin and sterile inflammation jointly drive fatal embryonic liver degeneration in endothelial chromatin remodeler mutant miceDevelopmentAugust 15, 2026Meng-Ling Wu, Yelyzaveta Rudenko, Courtney T. GriffinAB_2341188), anti-PECAM1 (1:100; BD Biosciences, 553370; RRID: AB_394816), anti-ICAM1 (1:200; R&D Systems, AF796; RRID: AB_2248703), anti-ECAD (1:200; Cell Signaling Technology, 3195; RRID: AB_2291471), anti-LYVE1

(2) Olfactory epithelium organoid models identify Ddit3 as a potential therapeutic target against inflammation-related olfactory sensory neuronal loss and functional deficitInternational Journal of Biological SciencesMarch 30, 2026Jinxia Liu, Jiaming Qi, Nan Jiang, Yuzhen Wang, Weihao Li, Shiyi Tian, Liujing Zhuang, Yunfeng Zhang, Yongliang Liu, Yiqun Yuanti-OMP (#ab183947, Abcam, 1:200), mouse anti-Ki67 (#550609, BD Biosciences, 1:200), goat anti-ICAM1 (#AF796, R&D Systems, 1:200), goat anti-IL33 (#AF-3626, R&D Systems, 1:200), rabbit anti-F4/80 (#GB113373, Servicebio,

(3) Geranylgeranyl Transferase Inhibitors Mitigate Intestinal Radiation ToxicityInternational journal of radiation oncology, biology, physicsMarch 15, 2026McGinness K, Al-Anbaky Q, Larrey EK, Cholia RP, Rotenberry M, Cook H, Pineda EN, Du R, Boerma M, Pathak R.(Proteintech 134709) diluted 1:2000, or anti- intercellular adhesion molecule 1 (ICAM-1) (R&D Systems AF796) diluted 1:4000. The next day, slides were washed and incubated for 45 min in biotinylated goat anti-rabbit

(4) Astrocyte CB1 receptors drive blood-brain barrier disruption in central nervous system inflammatory diseaseJournal of NeuroinflammationJanuary 29, 2026Teresa Colomer, Ana Bernal-Chico, Ester Sánchez-Martín, Alvaro Moreno-García, Andrés Mateo Baraibar, Aitziber Uribe-Irusta, Ander Iriarte-Sarria, Sandra Beriain, Urszula Skupio, Charlotte Gatuingt-Chasseriaud, et al.(CDH-5; 1:500; R&D Systems; #AF1002), Intercellular Adhesion Molecule 1 (ICAM-1; 1:500; R&D Systems; #AF796), Podocalyxin (PODXL; 1:500; R&D Systems; #AF1556), Vascular cell adhesion protein 1 (VCAM-1; 1:1000;

(5) RIPK3 Protects Against Endothelial Activation and Vascular Permeability in a Mouse Model of Ischemia-Reperfusion InjuryArterioscler Thromb Vasc BiolOctober 30, 2025Charmain F. Johnson, Christopher M. Schafer, Kathryn Y. Burge, Brian G. Coon, Hala Chaaban, Courtney T. Griffin(1:1000, R&D Systems; AF643), goat-anti-ICAM-1 (intracellular adhesion molecule-1; 1:2000, R&D Systems; AF796), rabbit-anti-NF-κB p65 (1:6000, Cell Signaling; 8242), rabbit-anti-phospho-NF-κB p65 (1:2000, Cell Signaling

(6) RIPK3 Protects Against Endothelial Activation and Vascular Permeability in a Mouse Model of Ischemia-Reperfusion InjuryArteriosclerosis, Thrombosis, and Vascular BiologyOctober 30, 2025Charmain F. Johnson, Christopher M. Schafer, Kathryn Y. Burge, Brian G. Coon, Hala Chaaban, Courtney T. Griffin(1:1000, R&D Systems; AF643), goat-anti-ICAM-1 (intracellular adhesion molecule-1; 1:2000, R&D Systems; AF796), rabbit-anti-NF-κB p65 (1:6000, Cell Signaling; 8242), rabbit-anti-phospho-NF-κB p65 (1:2000, Cell Signaling

(7) Plasmin activity and sterile inflammation synergize to promote lethal embryonic liver degenerationbioRxiv : the preprint server for biologySeptember 18, 2025Wu ML, Griffin CT.

(8) Selective deletion of interleukin-1 alpha in microglia does not modify acute outcome but may regulate neurorepair processes after experimental ischemic strokeJournal of Cerebral Blood Flow & MetabolismAugust 1, 2025Eloïse Lemarchand, Alba Grayston, Raymond Wong, Miyako Rogers, Blake Ouvrier, Benjamin Llewellyn, Freddie Webb, Nikolett Lénárt, Ádám Dénes, David Brough, et al.goat–anti-IL-1β (1 μg/mL, AF-401-NA; R&D Systems), goat–anti-Intercellular Adhesion Molecule 1 (ICAM-1) (1 μg/mL, AF796; R&D Systems), and chicken-anti-glial fibrillary acidic protein (GFAP) (1 μg/mL, Antibodies, ab4674)

(9) Autophagy hub-protein p62 orchestrates oxidative, ER stress and inflammatory responses post-ischemia/reperfusion exacerbating ischemic stroke outcomeRedox BiologyMay 27, 2025Xingyun Quan, Yukun Yang, Xiaolong Liu, Britta Kaltwasser, Matthias Pillath-Eilers, Bernd Walkenfort, Sylvia Voortmann, Ayan Mohamud Mohamud Yusuf, Nina Hagemann, Chen Wang, et al.(neuronal nuclei antigen; ab177487; Abcam), polyclonal goat anti-intercellular adhesion molecule-1 (ICAM1) (AF796; R&D Systems, Minneapolis, MN, USA), monoclonal rat anti-CD45 (05–1416, Merck Millipore), monoclonal

(10) Deficiency of Aging‐Related Gene Chitinase‐Like 4 Impairs Olfactory Epithelium HomeostasisCell ProliferationMay 19, 2025Tingting Wu, Weihao Li, Liujing Zhuang, Jinxia Liu, Ping Wang, Ye Gu, Yongliang Liu, Yiqun Yuanti‐Krt14 (#10143–1‐AP, Proteintech), rabbit anti‐Sox2 (#AF‐2018, R&D Systems), goat anti‐Icam1 (#AF796, R&D Systems), rabbit anti‐Chil4 (#ab192029, Abcam), rabbit anti‐OMP (#ab183947, Abcam), mouse anti‐Tuj1

(11) Earlier onset of chemotherapy-induced neuropathic pain in females by ICAM-1-mediated accumulation of perivascular macrophagesScience advancesApril 18, 2025Chen L, Zou X, Liu CC, Yan P, Deng J, Wang C, Chen MY, Tang XQ, Shi JM, Xin WJ, et al.membrane was incubated overnight at 4°C with primary antibodies against ICAM-1 (1:1000; R&D Systems, AF796) and GAPDH (1:1000; Cell Signaling Technology, 2118). The blots were then incubated for 1 hour at room

(12) Increased Levels of Circulating Methylglyoxal Have No Consequence for Cerebral Microvascular Integrity and Cognitive Function in Young Healthy MiceMolecular neurobiologyApril 1, 2025Berends E, Vangrieken P, Amiri N, van de Waarenburg MPH, Scheijen JLJM, Hermes DJHP, Wouters K, van Oostenbrugge RJ, Schalkwijk CG, Foulquier S.Specified primary antibodies were incubated over-night at 4 °C: goat anti-ICAM1 (1:400, R&D systems AF796), rat anti-cluster differentiation 31 (CD31) (1:200, Dianova DIA-310), or mouse anti-zonula occludens

(13) Selective deletion of interleukin-1 alpha in microglia does not modify acute outcome but may regulate neurorepair processes after experimental ischemic strokeJ Cereb Blood Flow MetabMarch 20, 2025Eloïse Lemarchand, Alba Grayston, Raymond Wong, Miyako Rogers, Blake Ouvrier, Benjamin Llewellyn, Freddie Webb, Nikolett Lénárt, Ádám Dénes, David Brough, et al.goat–anti-IL-1β (1 μg/mL, AF-401-NA; R&D Systems), goat–anti-Intercellular Adhesion Molecule 1 (ICAM-1) (1 μg/mL, AF796; R&D Systems), and chicken-anti-glial fibrillary acidic protein (GFAP) (1 μg/mL, Antibodies, ab4674)

(14) Lung contusion complicated by pneumonia worsens lung injury via the inflammatory effect of alveolar small extracellular vesicles on macrophages and epithelial cellsJournal of Trauma and Acute Care SurgeryJanuary 1, 2025Keita Nakatsutsumi, Wooil Choi, William Johnston, Katie Pool, Dong Jun Park, Jessica L. Weaver, Raul Coimbra, Brian Eliceiri, Todd W. CostantiniDanvers, MA) for isolated sEV at a dilution of 1:2,000 with the primary antibodies anti-ICAM-1 (Cat# AF796, R&D Systems, Minneapolis, MN) or at a dilution of 1:2,000 β-actin (Cat# 3700, CST, Danvers, MA) for

(15) Single-cell transcriptomic atlas of taste papilla agingAging cellDecember 1, 2024Ren W, Li W, Cha X, Wang S, Cai B, Wang T, Li F, Li T, Xie Y, Xu Z, et al.anti‐NTPDase2 (Centre de Recherche du CHUL, 1:500, #AB_2314986), goat anti‐ICAM1 (R&D Systems, 1:500, #AF796), rabbit anti‐Krt5 (Abcam, 1:100, #AB_52635), mouse anti‐Muc5b (Abcam, 1:100, #AB_77995), mouse anti‐Txn1

(16) Endothelial PDGF-D contributes to neurovascular protection after ischemic stroke by rescuing pericyte functionsCellular and Molecular Life SciencesDecember 1, 2024Maxime Bernard, Romain Menet, Sarah Lecordier, Ayman ElAli(1/250; R&D systems; AF2335), goat anti-intercellular adhesion molecule (ICAM)1 (1/250; R&D systems; AF796), rat anti-glial fibrillary acidic protein (GFAP) (1/500; Invitrogen, MA, USA; 13–0300), rabbit anti-aquaporin

(17) Lung Contusion Complicated by Pneumonia Worsens Lung Injury via the Inflammatory Effect of Alveolar Small Extracellular Vesicles on Macrophages and Epithelial CellsJ Trauma Acute Care SurgNovember 29, 2024Keita Nakatsutsumi, Wooil Choi, William Johnston, Katie Pool, Dong Jun Park, Jessica L. Weaver, Raul Coimbra, Brian Eliceiri, Todd.W CostantiniDanvers, MA) for isolated sEV at a dilution of 1:2,000 with the primary antibodies anti-ICAM-1 (Cat# AF796, R&D Systems, Minneapolis, MN) or at a dilution of 1:2,000 β-actin (Cat# 3700, CST, Danvers, MA) for

(18) Endothelial RIPK3 minimizes organotypic inflammation and vascular permeability in ischemia-reperfusion injurybioRxiv : the preprint server for biologyNovember 26, 2024Johnson CF, Schafer CM, Burge KY, Coon BG, Chaaban H, Griffin CT.

(19) Characterization of focused ultrasound blood-brain barrier disruption effect on inflammation as a function of treatment parametersbioRxiv : the preprint server for biologyJuly 16, 2024Angolano C, Hansen E, Ajjawi H, Nowlin P, Zhang Y, Thunemann N, Ferran C, Todd N.

(20) Safety of Anti-Reelin Therapeutic Approaches for Chronic Inflammatory DiseasesCellsMarch 27, 2024Calvier L, Alexander A, Marckx AT, Kounnas MZ, Durakoglugil M, Herz J.0.03% Triton-X100 in PBS), incubated with primary antibody (ICAM-1, R&D Systems (Minneapolis, MN, USA), AF796; Iba1, Novus Biologicals (Littleton, CO, USA), NB100-1028) overnight at 4 °C in a humid chamber, washed

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ICAM-1/CD54 Antibody

ICAM-1/CD54 Antibody

ICAM-1/CD54 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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ICAM-1/CD54 Antibody from R&D Systems, a Bio-Techne Brand

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