uPAR Antibody AF534 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemuPAR Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF534
NCBI Gene AliasesU-PAR, URKR, Cd87, UPAR, CD87, u-PAR, uPAR
ApplicationsELISA Development (Detection),Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Frozen,In vivo assay,Blockade of Receptor-ligand Interaction,Neutralization,Immunocytochemistry,CyTOF-ready
TargetuPAR
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
HostGoat
IsotypeIgG
ReactivityBovine,Mouse,Transgenic Mouse
NCBI Full Gene Nameplasminogen activator, urokinase receptor
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse uPAR isoform 1, Leu24-Thr297, Accession # Q545X5
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse uPAR in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used for immunohistochemistry in mouse tissues?

Yes, the uPAR antibody has been shown to be effective in immunohistochemistry, specifically in formalin-fixed paraffin-embedded mouse kidneys, where it was utilized at a dilution of 1:200 following antigen retrieval protocols ().

What applications has this antibody been cited in, and how has it been utilized in research?

The uPAR antibody has been cited in various applications including Western blotting, ELISA, flow cytometry, and immunocytochemistry. For instance, it was used in flow cytometry analysis at a dilution of 1:100 and in Western blotting at a dilution of 1:1000 to assess uPAR expression in different experimental contexts ().

Is there any evidence of this antibody being used in studies related to glomerular disease?

Yes, the uPAR antibody has been utilized in studies investigating the role of soluble urokinase plasminogen activator receptor in glomerular diseases. It was employed in immunoblotting to analyze suPAR concentrations in mouse serum and to evaluate its potential as a biomarker in glomerular disease models ().

What species does this antibody react with, and has it been validated in any specific experimental models?

The uPAR antibody is reactive with mouse and bovine species. It has been validated in various experimental models, including studies on oral squamous cell carcinoma in mice, where it was used to assess uPAR expression and its implications for tumor progression ().

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) uPAR exhibits age- and region-dependent expression in the brains of mice with Alzheimer’s disease-like pathologyBrain ResearchSeptember 1, 2026Lauren E. Sarko, Ella Klaus, Viktoriya Bondarenko, Callista Secker, Mackenzie S. Mnuk, Kaitlyn M. Marino, Daniel C. Shippy, Tyler K. Ulland, Krishanu Saha, Marina E. Emborg, et al.0.05% Triton X-100. The sections were incubated overnight in primary antibody (uPAR, 1:500, R&D Systems AF534, lot DCL0521042), washed in dilution media containing diH2O, NaCl, Tris-HCl, and Triton X-100, and incubated

(2) A convergent uPAR-positive tumor ecosystem creates broad vulnerability to CAR T cell therapyCellMay 14, 2026Zeda Zhang, Yu-Jui Ho, Xin Fang, Minseo Kim, Marguerite Li, Wei Luan, Clemens Hinterleitner, Sascha Haubner, Friederike Kogel, Edwin C. Pratt, et al.(280618),R&D Systems,Cat# IC2105S; RRID: AB_3654986 Goat polyclonal Mouse uPAR Unconjugated,R&D Systems,Cat# AF534; RRID: AB_2165351 Rabbit monoclonal Myc-Tag (71D10),Cell Signaling CST,Cat# #2278; RRID: AB_490778 "Rabbit

(3) Protein kinase Cι dictates tumor trajectory, cell plasticity, and immune surveillance in lung adenocarcinomaCell reportsDecember 23, 2025Nguyen DT, Prieto LI, Zhang C, Li H, Khoor A, Weems C, Jamieson L, Jeganathan KB, Babu SR, Murray NR, et al.

(4) Virus-derived serpin reduces immuno-coagulopathic damage in murine colitis by targeting the urokinase-type plasminogen activator receptor (uPAR) and complementScientific ReportsDecember 19, 2025Alexandra R. Lucas, Henna Monder, Junior Enow, Cheyanne Woodrow, Laurel Spaccarelli, Aman Garg, Jessika Schlievert, Nora Elmadbouly, Aashika Dupati, Emily Aliskevich, et al.markers, fibrinogen antibody (1:200), factor X antibody (Abcam # ab34269, 1:200), and uPAR (R&D Systems, AF534,1:100) as well as complement MAC (C5b/9, Abcam, ab 55,811, 1:200). HRP conjugated secondary antibodies

(5) Viral anti-inflammatory serpin reduces immuno-coagulopathic pathology in SARS-CoV-2 mouse models of infectionEMBO molecular medicineSeptember 11, 2023Zhang L, Li YH, Kibler K, Kraberger S, Varsani A, Turk J, Elmadbouly N, Aliskevich E, Spaccarelli L, Estifanos B, et al.neutrophils. For IHC primary antibodies included C5b/9 antibody (Abcam, ab 55811, 1:200), uPAR (R&D Systems, AF534, 1:100), fibrinogen antibody (Abcam, ab34269, 1:200), factor X antibody (Abcam, ab196023, 1:200), and

(6) PEGylated Serp-1 Markedly Reduces Pristane-Induced Experimental Diffuse Alveolar Hemorrhage, Altering uPAR Distribution, and Macrophage InvasionFrontiers in cardiovascular medicineJanuary 1, 2021Guo Q, Yaron JR, Wallen JW 3rd, Browder KF, Boyd R, Olson TL, Burgin M, Ulrich P, Aliskevich E, Schutz LN, et al.93668, 1:200), iNOS (Abcam, ab15323, 1:100), C5b/9 antibody (Abcam, ab 55811), and uPAR (R&D Systems, AF534,1:100). HRP-conjugated secondary antibodies against rabbit or goat IgG were applied at a dilution of

(7) In vivo antitumor activity by dual stromal and tumor-targeted oncolytic measles virusesCancer gene therapyDecember 1, 2020Jing Y, Chavez V, Khatwani N, Ban Y, Espejo AP, Chen X, Merchan JR.H2O2 for 10 min. The slides were washed in PBS and incubated with anti-muPAR antibody (R&D Systems, #AF534, Minneapolis, MN) for 30 min at 37°C. After washing in PBS, the slides were developed with Anti-Goat HRP-DAB Cell & Tissue Staining Kit

(8) Senolytic CAR T cells reverse senescence-associated pathologiesNatureJuly 1, 2020Amor C, Feucht J, Leibold J, Ho YJ, Zhu C, Alonso-Curbelo D, Mansilla-Soto J, Boyer JA, Li X, Giavridis T, et al.

(9) Further Evidence That the Soluble Urokinase Plasminogen Activator Receptor Does Not Directly Injure Mice or Human PodocytesTransplantationJanuary 1, 2020Harel E, Shoji J, Abraham V, Miller L, Laszik ZG, King A, Dobi D, Szabo G, Hann B, Sarwal MM, et al.(FFPE) mouse kidneys using standardized immunoperoxidase protocol with goat anti-mouse uPAR antibody (AF534, R&D System) following antigen retrieval at 100°C in pH 8.0, a secondary antibody Rabbit anti-goat HRP,

(10) Fibrinolysis protease receptors promote activation of astrocytes to express pro-inflammatory cytokinesJournal of neuroinflammationDecember 6, 2019Pontecorvi P, Banki MA, Zampieri C, Zalfa C, Azmoon P, Kounnas MZ, Marchese C, Gonias SL, Mantuano E.ODN 1826, were from InvivoGen. Amiloride (AMD) was from Sigma-Aldrich. Mouse uPAR-specific antibody (cat. AF534) and control IgG (cat. AB105C) were from R&D Systems. α-Enolase-specific polyclonal antibody was from

(11) A Virus-Derived Immune Modulating Serpin Accelerates Wound Closure with Improved Collagen RemodelingJournal of clinical medicineOctober 4, 2019Zhang L, Yaron JR, Tafoya AM, Wallace SE, Kilbourne J, Haydel S, Rege K, McFadden G, Lucas AR.hydrogel alone. Another cohort of 10 mice were locally given 1 µg of anti-uPAR antibody (R&D Systems, AF534, Minneapolis, MN, USA) in 10 µL saline and 5 of them were given 1 µg of Serp-1 in 10 µL saline after

(12) uPAR isoform 2 forms a dimer and induces severe kidney disease in miceThe Journal of clinical investigationApril 2, 2019Wei C, Li J, Adair BD, Zhu K, Cai J, Merchant M, Samelko B, Liao Z, Koh KH, Tardi NJ, et al.

(13) Cleavage of the urokinase receptor (uPAR) on oral cancer cells: regulation by transforming growth factor - β1 (TGF-β1) and potential effects on migration and invasionBMC cancerMay 19, 2017Magnussen SN, Hadler-Olsen E, Costea DE, Berg E, Jacobsen CC, Mortensen B, Salo T, Martinez-Zubiaurre I, Winberg JO, Uhlin-Hansen L, et al.Inc. (Ashland, OR). Antibodies Antigen affinity-purified polyclonal goat anti-mouse uPAR antibody (AF534, lot no: DCL03112081, DCL0311021) was from R&D Systems (Minneapolis, MN). We have previously demonstrated

(14) Urokinase-type Plasminogen Activator (uPA) Binding to the uPA Receptor (uPAR) Promotes Axonal Regeneration in the Central Nervous SystemThe Journal of biological chemistryFebruary 17, 2017Merino P, Diaz A, Jeanneret V, Wu F, Torre E, Cheng L, Yepes M.

(15) A reassessment of soluble urokinase-type plasminogen activator receptor in glomerular diseaseKidney internationalMarch 1, 2015Spinale JM, Mariani LH, Kapoor S, Zhang J, Weyant R, Song PX, Wong HN, Troost JP, Gadegbeku CA, Gipson DS, et al.cell supernatant was used. This assay uses polyclonal goat IgG mouse uPAR antibody (R&D systems; Cat# AF534) for suPAR detection. The assays were performed as per the manufacturer’s protocol. suPAR standard protein

(16) Tumour microenvironments induce expression of urokinase plasminogen activator receptor (uPAR) and concomitant activation of gelatinolytic enzymesPloS oneJanuary 1, 2014Magnussen S, Hadler-Olsen E, Latysheva N, Pirila E, Steigen SE, Hanes R, Salo T, Winberg JO, Uhlin-Hansen L, Svineng G.chart in figure 3b. Antibodies Antigen affinity-purified polyclonal goat anti-mouse uPAR antibody (AF534) was from R&D Systems (Minneapolis, MN, USA) and used at 1∶100 in flow cytometer analysis, 1∶200 in immunohistochemistry

(17) The calcineurin-NFAT pathway allows for urokinase receptor-mediated beta3 integrin signaling to cause podocyte injuryJournal of molecular medicine (Berlin, Germany)December 1, 2012Zhang B, Shi W, Ma J, Sloan A, Faul C, Wei C, Reiser J, Yang Y, Liu S, Wang W.

(18) Antipermeability function of PEDF involves blockade of the MAP kinase/GSK/beta-catenin signaling pathway and uPAR expressionInvestigative ophthalmology & visual scienceJune 1, 2010Yang J, Duh EJ, Caldwell RB, Behzadian MA.

(19) Urokinase-type plasminogen activator receptor (uPAR) augments brain damage in a murine model of ischemic strokeNeuroscience lettersFebruary 13, 2008Nagai N, Okada K, Kawao N, Ishida C, Ueshima S, Collen D, Matsuo O.

(20) Inhibition of choroidal neovascularization by a peptide inhibitor of the urokinase plasminogen activator and receptor system in a mouse modelArchives of ophthalmology (Chicago, Ill. : 1960)December 1, 2004Das A, Boyd N, Jones TR, Talarico N, McGuire PG.

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(1)

4/19/2022Anti-muPAR From R&D Systems I RIbeiromuPAR antibody from R&D Systems was used to stain mouse brain tissue. Good specific staining, but at higher dilutions, the antibody didn't work as well.

uPAR Antibody

uPAR Antibody

uPAR Antibody

uPAR Antibody

uPAR Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (22)

Reviews

uPAR Antibody from R&D Systems, a Bio-Techne Brand

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