EpCAM/TROP1 Antibody AF960 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemEpCAM/TROP1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF960
ApplicationsAdhesion Assay,Bioassay,Capture Assay,CTC,CyTof,FACS,Flow Cytometry,Immunohistochemistry,Western Blot,Simple Western,Immunocytochemistry,CyTOF-ready,Cell Selection,ELISA Capture
HostGoat
IsotypeIgG
TargetEpCAM/TROP1
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman,Mouse
Quantity100 ug (also 25 ug)
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant human EpCAM/TROP-1, extracellular domain, Gln24-Lys265, Accession # CAA32870
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Nameepithelial cell adhesion molecule
NCBI Gene AliasesBer-Ep4, BerEp4, DIAR5, EGP-2, EGP314, EGP40, ESA, HNPCC8, KS1/4, KSA, LYNCH8, M4S1, MIC18, MK-1, MOC-31, TACSTD1, TROP1

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human EpCAM/TROP-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TROP-2 and approximately 26% cross-reactivity with recombinant mouse EpCAM/TROP-1 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study epithelial cell adhesion in cancer research?

Yes, the EpCAM/TROP1 antibody has been utilized in various studies to investigate epithelial cell adhesion, particularly in the context of cancer. For instance, it has been shown to be effective in capturing circulating tumor cells in prostate cancer, which can serve as a predictive biomarker for antibody-drug conjugates (Sperger et al., 2023).

What applications has this antibody been shown to work in?

The EpCAM/TROP1 antibody has been demonstrated to be effective in several applications, including Western blotting, ELISA, flow cytometry, immunocytochemistry, and immunohistochemistry. It has been cited in studies involving the differentiation of human induced pluripotent stem cells into pulmonary neuroendocrine cells, where it was used for immunofluorescence validation (Hor et al., 2020).

Is this antibody specific for human EpCAM, and how does it perform in cross-reactivity tests?

The EpCAM/TROP1 antibody specifically detects human EpCAM/TROP-1 with less than 1% cross-reactivity with recombinant human TROP-2 and approximately 26% cross-reactivity with recombinant mouse EpCAM/TROP-1, making it a reliable choice for studies focused on human samples.

How has this antibody been utilized in the context of extracellular vesicle research?

The EpCAM/TROP1 antibody has been employed in the immunomagnetic sequential ultrafiltration platform for the enrichment and purification of extracellular vesicles from biofluids, demonstrating its utility in isolating tumor-specific extracellular vesicles, which are important for non-invasive biomarker discovery (Zhang et al., 2021).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Pharmacological Inhibition of SP1 Reverses Cancer Stemness and Enhances Sorafenib Efficacy in Hepatocellular CarcinomaCellsMay 22, 2026Maël Padelli, Christophe Desterke, Aurore Devocelle, Denis Clay, Agnès Bourillon, Georges Uzan, Antoinette Lemoine, Julien Giron-MichelE-cadherin (BD Biosciences, San Jose, CA, USA, #610181, 1/1000), EpCam (Bio-Techne, Minneapolis, MN, USA, #AF960, 1/1000), vimentin (Cell signaling Technology, #73260, 1/1000), SOX2 (Bio-Techne, Minneapolis, MN, USA,

(2) Spatial tumor evolution panorama of ovarian cancerCell Rep MedMarch 17, 2026Chenzhao Feng, Yunyi Yang, Guanchu Li, Zixuan Fang, Bin Yang, Fang Zhao, Xingyu Chen, Shuyi Liu, Di Wu, Xingyuan Hu, et al.Biotechnology,Kit-0026 PCK,Maxim Biotechnology,RAB-0050 EPCAM for IHC,Abcam,ab213500 EPCAM for uDISCO,R&D Systems,AF960 SPP1 for uDISCO,Invitrogen,14-9096-82 APC-H7 anti-human CD45,BD Biosciences,560178 Alexa Fluor Plus

(3) Hydrogen sulfide rescues IFNγ/TNFα-induced intestinal epithelial barrier dysfunction by enhancing oxidative phosphorylationScientific ReportsFebruary 23, 2026Janaíne Prata de Oliveira, Matthias van Sligtenhorst, Cansu Akkaya, Astrid Verbiest, Victoria Francelino Alves, Marie Wallays, Schalk van der Merwe, Marc Ferrante, Tim Vanuytsel, John L. Wallace, et al.cat. #PA5-51,548, 1:50) and goat anti-epithelial cell adhesion molecule (EPCAM; R&D Systems, cat. #AF960, 1:100). After primary incubation, the sections were incubated with secondary antibodies conjugated

(4) Organoid platinum-resistance model identifies KRT17 as a biomarker of targeted therapy in ovarian canceriScienceDecember 19, 2025Juliane Reichenbach, Juliana Schmid, Sophia Hierlmayer, Tingyu Zhang, Ilaria Piga, Sophia Geweniger, Jonas Fischer, Aarushi Davesar, Nemanja Vasovic, Anca Chelariu-Raicu, et al.REAGENT or RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, EpCam,R&D-Systems,Cat#Af960; RRID: AB_355745 gamma-H2A.X,Abcam,Cat#ab81299; RRID: AB_1640564 pBRCA1,Abcam,Cat#ab47325; RRID: AB_868469 E-Cadherin,BD,Cat#610181;

(5) Design of CellProfiler-based Pipelines Enabling the Attribution of Molecular Stress Markers to Specific Tissue and Subcellular Compartments of the Colonic MucosaCellular and Molecular Gastroenterology and HepatologyDecember 5, 2025Helena Hödlmayr, Christina Watschinger, Gerald K. Wallner, Sabine Knipp, Arndt Rohwedder, Regina Prommer, Rupert Langer, Alexander R. Moschenantibodies used were: rabbit anti-G3BP1 (1:200, HPA004052, Sigma Life Science), and goat anti-hEpCAM (1:200, AF960, R&D Systems). Additional human tissue specimens, obtained as residual material from the Department

(6) Establishing and Maintaining 3D Organoid Cultures From Human Fallopian Tube EpitheliumBio ProtocAugust 20, 2025David Holthaus, Nina Hedemann, Sophia Theresa Geweniger, Huy Duc Le, Ibrahim Alkatout, Mirjana Kessler, Thomas F. Meyerbars represent 20 μm. The following antibodies were used in this experiment: goat anti-EPCAM (1:200, Af960, R&D Systems), mouse anti-acetylated Tubulin (1:1,000, T7451-25, Sigma), rabbit anti-PAX8 (1:200, 10336-1-AP,

(7) Establishing and Maintaining 3D Organoid Cultures From Human Fallopian Tube EpitheliumBIO-PROTOCOLAugust 20, 2025David Holthaus, Nina Hedemann, Sophia Theresa Geweniger, Huy Duc Le, Ibrahim Alkatout, Mirjana Kessler, Thomas F. Meyerbars represent 20 μm. The following antibodies were used in this experiment: goat anti-EPCAM (1:200, Af960, R&D Systems), mouse anti-acetylated Tubulin (1:1,000, T7451-25, Sigma), rabbit anti-PAX8 (1:200, 10336-1-AP,

(8) Cellular crosstalk mediated by TGF-β drives epithelial-mesenchymal transition in patient-derived multi-compartment biliary organoidsNat CommunJuly 17, 2025Hiroaki Ayabe, Erica A. K. DePasquale, Surya P. Amarachintha, Reena Mourya, Wenqi Li, Shreya Nalluri, Sejal R. Fox, Kenichiro Konishi, Pranavkumar Shivakumar, Jorge A. Bezerrawere incubated with PE-conjugated TROP2 (BioLegend, 363804) and EPCAM antibodies (R and D Systems, AF960) in FACS buffer after the live/dead staining described above. After washing, EPCAM-specific signals

(9) Protocol to infect differentiated human primary bronchial epithelial cells with live mycobacteria and determine intracellular loadSTAR ProtocJune 20, 2025Amy M. Barclay, Kimberley V. Walburg, Dennis K. Ninaber, Tom H.M. Ottenhoff, Pieter S. Hiemstra, Anne M. van der Does, Simone A. Joostendiluted),Sigma-Aldrich,HPA031828 Human EpCAM/TROP-1 antibody produced in goat (used 20 times diluted),R&D Systems,AF960 "Monoclonal anti-tubulin, acetylated antibody produced in mouse (used 200 times diluted)",Sigma-Aldrich,T6793 "Lab

(10) Protocol for development, visualization, and quantification of mycobacterial biofilms on primary human airway epithelial cellsSTAR ProtocJune 20, 2025Amy M. Barclay, Ronald W.A.L. Limpens, Montserrat Bárcena, Tom H.M. Ottenhoff, Pieter S. Hiemstra, Anne M. van der Does, Simone A. JoostenRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Human EpCAM/TROP-1 antibody produced in goat (used 20 times diluted),R&D Systems,AF960 Donkey-anti-goat IgG (H+L) highly cross-adsorbed secondary antibody Alexa Fluor 405 (used 200 times

(11) A spatiotemporal and machine-learning platform facilitates the manufacturing of hPSC-derived esophageal mucosaDevelopmental CellMay 1, 2025Ying Yang, Carmel Grace McCullough, Lucas Seninge, Lihao Guo, Woo-Joo Kwon, Yongchun Zhang, Nancy Yanzhe Li, Sadhana Gaddam, Cory Pan, Hanson Zhen, et al.anti-ECAD,Millipore Sigma,Cat# MAB3199Z; RRID: AB_347700 Goat polyclonal anti-EPCAM,R&D systems,Cat# AF960; RRID: AB_355745 Rabbit monoclonal anti-KRT13,Abcam,Cat# ab92551; RRID: AB_2134681 Mouse monoclonal

(12) Time-series single-cell transcriptomic profiling of luteal-phase endometrium uncovers dynamic characteristics and its dysregulation in recurrent implantation failuresNature communicationsJanuary 2, 2025Cao D, Liu Y, Cheng Y, Wang J, Zhang B, Zhai Y, Zhu K, Liu Y, Shang Y, Xiao X, et al.h. Next, the slides were incubated with diluted primary antibodies against EPCAM (1:200 dilutions, #AF960, R&D Systems), CDH1 (1:200 dilutions, #20874-1-AP, proteintech), EDG7 (1:100 dilutions, #19509-1-AP,

(13) Mycobacteria develop biofilms on airway epithelial cells and promote mucosal barrier disruptioniScienceNovember 15, 2024Amy M. Barclay, Dennis K. Ninaber, Ronald W.A. L. Limpens, Kimberley V. Walburg, Montserrat Bárcena, Pieter S. Hiemstra, Tom H.M. Ottenhoff, Anne M. van der Does, Simone A. JoostenAntibody (C13),Invitrogen,Cat#MA5-41552; RRID:AB_2899035 Human EpCAM/TROP-1 Antibody,R&D Systems,Cat#AF960; RRID:AB_355745 ,, Bacterial and virus strains,Bacterial and virus strains,Bacterial and virus strains ,, Mycobacterium avium

(14) Transcriptomic Profile of Breast Tissue of Premenopausal Women Following Treatment with Progesterone Receptor Modulator: Secondary Outcomes of a Randomized Controlled TrialInternational journal of molecular sciencesJuly 10, 2024Utjés D, Boggavarapu NR, Rasul MF, Koberg I, Zulliger A, Ponandai-Srinivasan S, von Grothusen C, Lalitkumar PG, Papaikonomou K, Alkasalias T, et al.(CD49f, Rat, #MA5-16884, ThermoFisher Scientific (Waltham, MA, USA); EPCAM, Goat, R and D Systems Cat# AF960, RRID:AB 355745; CK8, Mouse, Santacruz, sc-8020; CK14, Rabbit, Invitrogen, # MA5-32214) in 2% BSA in

(15) Studies with Human-Induced Pluripotent Stem Cells Reveal That CTNS Mutations Can Alter Renal Proximal Tubule DifferentiationInternational journal of molecular sciencesNovember 30, 2023Thiyagarajan R, Taub M.ectoderm), rabbit anti-brachyury (R&D Systems MAP20851; mesoderm), and goat anti-EpCam (R&D Systems AF960; endoderm). Following the primary antibody incubation, slides were washed and incubated with secondary

(16) Cryptotanshinone is a candidate therapeutic agent for interstitial lung disease associated with a BRICHOS-domain mutation of SFTPCiScienceOctober 20, 2023Hosokawa M, Mikawa R, Hagiwara A, Okuno Y, Awaya T, Yamamoto Y, Takahashi S, Yamaki H, Osawa M, Setoguchi Y, et al.

(17) Early-onset tufting enteropathy in HAI-2-deficient mice is independent of matriptase-mediated cleavage of EpCAMDevelopment (Cambridge, England)September 1, 2023Szabo R, Kawaguchi M, Kataoka H, Bugge TH.Cell Signaling Technology), mouse anti-Myc tag (2276, Cell Signaling Technology) or goat anti-EpCAM (AF960, R&D Systems) antibody and 50 µl of Pierce Protein A/G Plus Agarose beads for 16 h at 4°C. The samples

(18) IL8 derived from macrophages inhibits CD8(+) T-cell function by downregulating TIM3 expression through IL8-CXCR2 axis in patients with advanced colorectal cancerInternational immunopharmacologyAugust 1, 2023Zhao C, Wang D, Li Z, Zhang Z, Xu Y, Liu J, Lei Q, Han D, Huo Y, Liu S, et al.

(19) A distal lung organoid model to study interstitial lung disease, viral infection and human lung developmentNature protocolsJuly 1, 2023Matkovic Leko I, Schneider RT, Thimraj TA, Schrode N, Beitler D, Liu HY, Beaumont K, Chen YW, Snoeck HW.9664S),AB_2070042,1:400 COL4,Mouse,COL-94,Abcam (cat. no. ab6311),AB_305414,1:100 EPCAM,Goat,Polyclonal,R&D Systems (cat. no. AF960),AB_355745,1:400 FOXA1,Mouse,Q-6,Santa Cruz Biotechnology (cat. no. sc-101058),AB_1124659,1:50 HT2-280,Mouse,TB-27,Terrace

(20) Expression and Therapeutic Targeting of TROP-2 in Treatment-Resistant Prostate CancerClinical cancer research : an official journal of the American Association for Cancer ResearchJune 13, 2023Sperger JM, Helzer KT, Stahlfeld CN, Jiang D, Singh A, Kaufmann KR, Niles DJ, Heninger E, Rydzewski NR, Wang L, et al.paramagnetic particles Goat polyclonal antibodies to the epithelial cell adhesion molecule (R&D Systems Cat# AF960, RRID:AB_355745) and TROP-2 (R&D Systems Cat# AF650, RRID:AB_2205667) were biotinylated according to

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