TIMP-1 Antibody AF970 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemTIMP-1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF970
ApplicationsImmunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Proximity Ligation Assay,In vivo assay,Simple Western,Neutralization
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
TargetTIMP-1
Quantity100 ug (also 25 ug)
ReactivityHuman,Mouse,Transgenic Mouse
IsotypeIgG
HostGoat
Conjugate/TagUnconjugated
NCBI Full Gene NameTIMP metallopeptidase inhibitor 1
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant human TIMP-1, Cys24-Ala207, Accession # Q6FGX5
ConcentrationLYOPH
PurityAntigen Affinity-purified
ValidationKnockout Validated
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
Antibody TypePrimary Antibodies
NCBI Gene AliasesTIMP, TIMP-1, EPO, CLGI, HCI, EPA

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human TIMP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse TIMP-1 and recombinant rat TIMP-1 is observed and less than 1% cross-reactivity with recombinant human (rh) TIMP-2, rhTIMP-3, and rhTIMP-4 is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Generation of Self‐Organizing Macrovascular Constructs by Bioprinting Human iPSC‐Derived Mesodermal Progenitor CellsAdvanced ScienceJune 9, 2026Leyla E. Dogan, Nathaly A. Chicaiza‐Cabezas, Florian Kleefeldt, Philipp Wörsdörfer, Jürgen Groll, Süleyman ErgünCD45 (13‐9457‐82, Invitrogen), IBA1 (019‐19741, Wako/Fuji Film, Neuss, Germany) and eNOS (R&D Systems, AF970, Germany). After washing three times in PBS, sections were incubated for 1 h at RT with fluorophore‐conjugated

(2) TIMP1 Overexpression in Ovarian Cancer Spheroids: Implications for Prognosis, Resistance, and Metastatic PotentialCancers (Basel)May 9, 2025Andrea Jemma, Alessandra Ardizzoia, Chiara Villa, Sara Bonomo, Mario Mauri, Carla Reale, Concetta Ambrosino, Massimiliano Cadamuro, Marialuisa Lavitrano, Donatella Conconistored in aliquots at −80 °C. Recombinant Human TIMP-1 Protein, CF (970-TM) and Human TIMP-1 Antibody (AF970) were both purchased by Bio-techne (Minneapolis, MN, USA) and reconstituted in deionized water and PBS,

(3) The periosteum provides a stromal defence against cancer invasion into the boneNatureOctober 1, 2024Nakamura K, Tsukasaki M, Tsunematsu T, Yan M, Ando Y, Huynh NC, Hashimoto K, Gou Q, Muro R, Itabashi A, et al.

(4) Inhibition of Monoacylglycerol Lipase Decreases Angiogenic Features of Endothelial Cells via Release of Tissue Inhibitor of Metalloproteinase-1 from Lung Cancer CellsCellsJune 30, 2023Wittig F, Henkel L, Prüser JL, Merkord J, Ramer R, Hinz B.Biosciences). Primary TIMP-1 antibody was purchased from R&D Systems (Wiesbaden-Nordenstadt, Germany; #AF970, RRID:AB_355751). The secondary antibody for TIMP-1 was a polyclonal biotin-labeled goat anti-rat IgG

(5) Myeloma Microenvironmental TIMP1 Induces the Invasive Phenotype in Fibroblasts to Modulate Disease ProgressionInternational journal of molecular sciencesJanuary 22, 2023Ishihara R, Oda T, Murakami Y, Matsumura I, Watanabe S, Asao Y, Masuda Y, Gotoh N, Kasamatsu T, Takei H, et al.Materials. 4.2. Reagents Recombinant TIMP1 (Peprotech, Rocky Hill, NJ, USA), neutralizing anti-TIMP1 antibody (AF970; R&D Systems, Minneapolis, MN, USA), anti-myeloma reagents bortezomib (Selleck Chemicals, Houston, TX,

(6) Median raphe serotonergic neurons projecting to the interpeduncular nucleus control preference and aversionNature communicationsDecember 22, 2022Kawai H, Bouchekioua Y, Nishitani N, Niitani K, Izumi S, Morishita H, Andoh C, Nagai Y, Koda M, Hagiwara M, et al.(Tph) antibody (1:200; AB1541, Merck Millipore), goat polyclonal anti-SERT antibody (1:200; HTT-Go-Af970, Nittobo Medical), mouse monoclonal anti-c-Fos antibody (2H2) (1:200; NBP2-50037, Novus Biologicals),

(7) Inhibition of epithelial-mesenchymal transition in retinal pigment epithelial cells by a retinoic acid receptor-α agonistScientific reportsJune 4, 2021Kobayashi Y, Tokuda K, Yamashiro C, Higashijima F, Yoshimoto T, Ota M, Ogata T, Ashimori A, Hatano M, Kobayashi M, et al.

(8) Heroin Seeking and Extinction From Seeking Activate Matrix Metalloproteinases at Synapses on Distinct Subpopulations of Accumbens CellsBiological psychiatryMay 15, 2021Chioma VC, Kruyer A, Bobadilla AC, Angelis A, Ellison Z, Hodebourg R, Scofield MD, Kalivas PW.reagents Heroin HCl (NIDA), was dissolved in sterile saline. TIMP-1 and −2 antibodies (R&D Systems, #AF970 and #AF971, respectively) or normal Goat IgG (R&D Systems, #AB-108-C) were microinjected (29, 30). MMP-2i

(9) IL35 predicts prognosis in gastric cancer and is associated with angiogenesis by altering TIMP1, PAI1 and IGFBP1FEBS open bioDecember 1, 2020Li X, Niu N, Sun J, Mou Y, He X, Mei L.

(10) The Metalloproteinase ADAMTS5 Is Expressed by Interstitial Inflammatory Cells in IgA Nephropathy and Is Proteolytically Active on the Kidney MatrixJournal of immunology (Baltimore, Md. : 1950)October 15, 2020Taylor S, Whitfield M, Barratt J, Didangelos A.

(11) Circulating Tumor Cells Characterization Revealed TIMP1 as a Potential Therapeutic Target in Ovarian CancerCellsMay 14, 2020Abreu M, Cabezas-Sainz P, Alonso-Alconada L, Ferreirós A, Mondelo-Macía P, Lago-Lestón RM, Abalo A, Díaz E, Palacios-Zambrano S, Rojo-Sebastian A, et al.efficacy of the silencing, WB was performed in 10% acrylamide gels, using anti-TIMP1 antibody 1:150 (AF970; R&D Systems, Minneapolis, MN, USA) to incubate the blots overnight at 4 ◦ C. Afterwards, the blots

(12) Looking for a Better Characterization of Triple-Negative Breast Cancer by Means of Circulating Tumor CellsJournal of clinical medicineJanuary 27, 2020Abreu M, Cabezas-Sainz P, Pereira-Veiga T, Falo C, Abalo A, Morilla I, Curiel T, Cueva J, Rodríguez C, Varela-Pose V, et al.and AKT status was assessed by Western blot in 10% acrylamide gels, using anti-TIMP1 antibody 1:150 (AF970; R&D Systems, MN, USA), anti-AKT antibody 1:1000 (40D4, Cell Signaling, MA, USA), anti-pAKT 1:1000 (4060S,

(13) SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent CellsCell reportsSeptember 24, 2019Wiley CD, Liu S, Limbad C, Zawadzka AM, Beck J, Demaria M, Artwood R, Alimirah F, Lopez-Dominguez JA, Kuehnemann C, et al.(AF2609), SERPINB2 (MAB85501), SERPINB6 (AF4336), SERPINE1 (MAB1786), TFPI (AF2974), THBS1 (AF3074), TIMP1 (AF970), VCL (MAB6896). SA-β-Gal and BrdU Labeling We detected SA-β-Gal as described (Dimri et al., 1995),

(14) Higher levels of TIMP-1 expression are associated with a poor prognosis in triple-negative breast cancerMolecular cancerApril 30, 2016Cheng G, Fan X, Hao M, Wang J, Zhou X, Sun X.mice were injected with a neutralizing antibody against TIMP-1 (10 μg per 25 g of body weight) (Cat. #AF970, R&D Systems) or the IgG control, and the injections were repeated once a week for 4 weeks. Tumor volumes

(15) Targeting TR4 nuclear receptor suppresses prostate cancer invasion via reduction of infiltrating macrophages with alteration of the TIMP-1/MMP2/MMP9 signalsMolecular cancerJanuary 27, 2015Ding X, Yang DR, Xia L, Chen B, Yu S, Niu Y, Wang M, Li G, Chang C.incubated at 37°C in a humidified incubator at 5% CO2. Antibodies Anti-TR4 (PP-0107B-00) and anti-TIMP-1 (AF970) antibodies were purchased from R&D systems (Minneapolis, MN). Anti-GAPDH (6c5) antibody was purchased

(16) Plasma levels of the MMP-9:TIMP-1 complex as prognostic biomarker in breast cancer: a retrospective studyBMC cancerDecember 13, 2013Thorsen SB, Christensen SL, Würtz SO, Lundberg M, Nielsen BS, Vinther L, Knowles M, Gee N, Fredriksson S, Møller S, et al.affinity purified polyclonal antibody (MMP-9 (R&D Systems, Cat.no. AF911) or TIMP-1 (R&D Systems, Cat.no. AF970)) to 3’-hydroxyl free and 5’-phosphate free 40-mer oligonucleotide sequences (Figure 1). Thereby, the

(17) Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growthNature medicineOctober 30, 2011Nieman KM, Kenny HA, Penicka CV, Ladanyi A, Buell-Gutbrod R, Zillhardt MR, Romero IL, Carey MS, Mills GB, Hotamisligil GS, et al.control (MAB002). Mice were pretreated with 100 μg per kg body weight TIMP-1–specific (R&D Systems AF970) or goat IgG (R&D Systems AB108C) antibodies 30 min before cancer cell injection. Labeled SKOV3ip1 cells

(18) Breast cancer cells induce cancer-associated fibroblasts to secrete hepatocyte growth factor to enhance breast tumorigenesisPloS oneJanuary 13, 2011Tyan SW, Kuo WH, Huang CK, Pan CC, Shew JY, Chang KJ, Lee EY, Lee WH.

(19) Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulationEuropean journal of haematologyMarch 1, 2010Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O.

(20) Fibroblast-conditioned media promote human sarcoma cell invasionSurgeryJanuary 1, 2009Bittner JG 4th, Wilson M, Shah MB, Albo D, Feig BW, Wang TN.

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Human TIMP-1 Antibody

TIMP-1 Antibody

TIMP-1 Antibody

TIMP-1 Antibody

TIMP-1 Antibody

TIMP-1 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (25)

Reviews

TIMP-1 Antibody from R&D Systems, a Bio-Techne Brand

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