IGFBP-2 Antibody AF674 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemIGFBP-2 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF674
ApplicationsWestern Blot,Simple Western,Neutralization
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Quantity100 ug (also 25 ug)
ReactivityFeline,Human,Mouse
IsotypeIgG
TargetIGFBP-2
HostGoat
Conjugate/TagUnconjugated
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant human IGFBP-2, Glu40-Gln328, Accession # CAA34373
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Nameinsulin like growth factor binding protein 2
NCBI Gene AliasesIBP2, IGF-BP53

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human IGFBP-2 in direct ELISAs. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) IGFBP-1 and rmIGFBP-2 and less than 1% cross-reactivity with recombinant human IGFBP-1, -3, -4, -5, -7 and rmIGFBP-3, -5, -6, and -7 is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Aortic valve stenosis serum IGFBP2 mediates female-specific Evogliptin resistance in valve myofibroblastsCell ReportsJuly 28, 2026Brandon J. Vogt, Megan Chavez, Nicole E. Félix Vélez, Rayyan M. Gorashi, Ryan R. Reeves, Brian A. Aguadowell. Neutralizing antibody experiments Human IGFBP2 neutralizing antibody (R&D systems, Cat. No AF674, RRID: AB_355517) and normal IgG control antibody (R&D systems, Cat. No AB-108-C, RRID: AB_354267) were

(2) Targeting hypoxic exosomal IGFBP2 overcomes CD47-mediated immune evasion in glioblastomaCell death & diseaseJanuary 31, 2026Qi Y, Zhao R, Zhang X, Xia H, Zhang P, Wang Q, Zhao S, Wang S, Zhao H, Guo X, et al.Treatments included tail vein injections of anti-CD47 reagent (BE0019, BioXcell), anti-IGFBP2 antibody (#AF674, R&D Systems), or isotype control antibody (#BP0089, BioXcell) three times a week after tumor implantation.

(3) Mutant PP2A Induces IGFBP2 Secretion to Promote Development of High-Grade : Uterine CancerCancer ResFebruary 1, 2025Terrance J. Haanen, Sophie Boock, Catherine G. Callahan, Irene Peris, Kaitlin P. Zawacki, Brynne Raines, Charles A. Nino, Brian Tran, Alexis Harold, Gabrielle Hodges Onishi, et al.at 4°C for immediate use. For cell-based assays, IGFBP2 neutralizing antibody (R&D systems, catalog #AF674) was received and stored at 4°C for immediate use. Limiting dilution analysis in vitro Cells were

(4) Mutant PP2A Induces IGFBP2 Secretion to Promote Development of High-Grade : Uterine CancerCancer ResearchFebruary 1, 2025Terrance J. Haanen, Sophie Boock, Catherine G. Callahan, Irene Peris, Kaitlin P. Zawacki, Brynne Raines, Charles A. Nino, Brian Tran, Alexis Harold, Gabrielle Hodges Hodges Onishi, et al.at 4°C for immediate use. For cell-based assays, IGFBP2 neutralizing antibody (R&D systems, catalog #AF674) was received and stored at 4°C for immediate use. Limiting dilution analysis in vitro Cells were

(5) Age-Related Increases in IGFBP2 Increase Melanoma Cell Invasion and Lipid SynthesisCancer Res CommunAugust 1, 2024Gretchen M. Alicea, Payal Patel, Marie E. Portuallo, Mitchell E. Fane, Meihan Wei, Yash Chhabra, Agrani Dixit, Alexis E. Carey, Vania Wang, Murilo R. Rocha, et al.674-B2-025 from R&D Systems) or aged CM in the presence or absence of 5 mg/mL neutralizing IGFBP2 antibody (AF674 from R&D Systems) were seeded on top. After 18 hours of incubation at 37°C, 5% CO2, non-invading cells

(6) Age-Related Increases in IGFBP2 Increase Melanoma Cell Invasion and Lipid SynthesisCancer Research CommunicationsAugust 1, 2024Gretchen M. Alicea, Payal Patel, Marie E. Portuallo, Mitchell E. Fane, Meihan Wei, Yash Chhabra, Agrani Dixit, Alexis E. Carey, Vania Wang, Murilo R. Rocha, et al.674-B2-025 from R&D Systems) or aged CM in the presence or absence of 5 mg/mL neutralizing IGFBP2 antibody (AF674 from R&D Systems) were seeded on top. After 18 hours of incubation at 37°C, 5% CO2, non-invading cells

(7) Transcriptomic Analysis of Extracellular Vesicles in the Search for Novel Plasma and Thrombus Biomarkers of Ischemic Stroke EtiologiesInternational journal of molecular sciencesApril 16, 2024Machado FJDM, Marta-Enguita J, Gómez SU, Rodriguez JA, Páramo-Fernández JA, Herrera M, Zandio B, Aymerich N, Muñoz R, Bermejo R, et al.(20 ng/mL, Sigma) in the presence or absence of a neutralizing antibody against IGFBP-2 (600 ng/mL, AF674, R and D, following the manufacturer’s neutralization dosage recommendation) in SFMEC for 2 h. In the

(8) Age-related increases in IGFBP2 increase melanoma cell invasion and lipid synthesisbioRxiv : the preprint server for biologyMay 2, 2023Alicea GM, Portuallo ME, Patel P, Fane ME, Carey AE, Speicher D, Tang HY, Kossenkov AV, Rebecca VW, Wirtz DG, et al.

(9) IGFBP2 promotes proliferation and cell migration through STAT3 signaling in Sonic hedgehog medulloblastomaActa neuropathologica communicationsApril 8, 2023Kunhiraman H, McSwain L, Shahab SW, Gershon TR, MacDonald TJ, Kenney AM.(IGFBP2 nAb) and control Goat IgG were applied to the cell lines in serum free medium (2.5–8 µg/ml) (#AF674 R&D systems). Recombinant IGFBP2 (Abcam) was applied to cells cultured in serum free media. STAT3 inhibitor

(10) Metallothionein 2A Expression in Cancer-Associated Fibroblasts and Cancer Cells Promotes Esophageal Squamous Cell Carcinoma ProgressionCancersSeptember 10, 2021Shimizu M, Koma YI, Sakamoto H, Tsukamoto S, Kitamura Y, Urakami S, Tanigawa K, Kodama T, Higashino N, Nishio M, et al.RPMI with 0.1% FBS were seeded in the lower chamber with/without Human IGFBP2 Antibody (2.5 μg/mL, AF674; R&D systems) or Normal Goat IgG Control (2.5 μg/mL, AB-108-C; R&D systems) as the negative control.

(11) A protumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinomaThe Journal of clinical investigationJanuary 4, 2021Tan X, Shi L, Banerjee P, Liu X, Guo HF, Yu J, Bota-Rabassedas N, Rodriguez BL, Gibbons DL, Russell WK, et al.II (05-623, clone CTD4H8) from MilliporeSigma. We purchased neutralizing antibodies against IGFBP2 (AF674) and SPP1 (AF1433) from R&D Systems. We purchased recombinant IGFBP2 (ab63223) and SPP1 (ab92964) proteins

(12) Intracellular Insulin-like growth factor binding protein 2 (IGFBP2) contributes to the senescence of keratinocytes in psoriasis by stabilizing cytoplasmic p21AgingApril 17, 2020Mercurio L, Lulli D, Mascia F, Dellambra E, Scarponi C, Morelli M, Valente C, Carbone ML, Pallotta S, Girolomoni G, et al.IGFBP2 bioactivity in keratinocyte supernatants, the neutralizing goat polyclonal anti-human IGFBP2 Ab (AF674) or goat IgG control (AB-108-C) from R&D Systems was used. The exact dose of anti-IGFBP2 Ab to obtain

(13) Mechanisms utilized by feline adipose-derived mesenchymal stem cells to inhibit T lymphocyte proliferationStem cell research & therapyJune 25, 2019Taechangam N, Iyer SS, Walker NJ, Arzi B, Borjesson DL.clone 9106, R&D systems), or interferon gamma (IFNγ, 15 μg/mL, goat anti-feline polyclonal antibody #AF674, R&D systems), or chemicals to block prostaglandin E2 (PGE2, 10 μm/mL, indomethacin, Cayman Chemical),

(14) Glioma progression is mediated by an addiction to aberrant IGFBP2 expression and can be blocked using anti-IGFBP2 strategiesThe Journal of pathologyJuly 1, 2016Phillips LM, Zhou X, Cogdell DE, Chua CY, Huisinga A, R Hess K, Fuller GN, Zhang W.serum-free media for the indicated times. IGFBP2-neutralizing antibody (0.1 – 10 ug/mL) (R&D Systems #AF674) or control goat IgG (2 or 10 ug/mL) were also administered to cells in serum-free media. Results IGFBP2

(15) Insulin-like growth factor-binding protein 2 secreted by a tumorigenic cell line supports ex vivo expansion of mouse hematopoietic stem cellsStem CellsJune 1, 2008Huynh H, Iizuka S, Kaba M, Kirak O, Zheng J, Lodish HF, Zhang CC.

(16) The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pyloriGastroenterologyMay 1, 2006McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, et al.

(17) Fibrocytes boost tumor-supportive phenotypic switches in the lung cancer niche via the endothelin systemNature communicationsJanuary 1, 0001"Weigert A, Zheng X, Nenzel A, Turkowski K, Günther S, Strack E, Sirait-Fischer E, Elwakeel E, Kur IM, Nikam VS, et al."μg/mL R&D Systems #AB-221-NA), VEGF (0.1 μg/mL R&D Systems #MAB293-100), IGFBP-2 (2 μg/mL R&D Systems #AF674), and IGFBP-3 (1 μg/mL R&D Systems #AF675) were depleted individually from conditioned medium of A549-fibrocyte

(18) Secreted PGK1 and IGFBP2 contribute to the bystander effect of miR-10b gene editing in gliomaMolecular therapy. Nucleic acidsJanuary 1, 0001"Zhang Y, Rabinovsky R, Wei Z, El Fatimy R, Deforzh E, Luan B, Peshkin L, Uhlmann EJ, Krichevsky AM."corresponding control cultures were incubated with 10 μg/mL IGFBP2-neutralizing antibody (R&D Systems, AF674)55 or control goat immunoglobulin G (IgG; Sigma) at 37°C for 4 h. The antibody has been removed from

(19) IGFBP2 secretion by mammary adipocytes limits breast cancer invasionScience advancesJanuary 1, 0001"Conway JRW, Dinç DD, Follain G, Paavolainen O, Kaivola J, Boström P, Hartiala P, Peuhu E, Ivaska J."R-03729-E10). Primary antibodies in AdvanBlock-Fluor were incubated overnight at 4°C; IGFBP2 (R&D Systems, AF674), IGF-II (Millipore, 05-166-MI), GAPDH (Hytest, 5G4MAB6C5), and GFP (Thermo Fisher Scientific, A11122).

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Human IGFBP-2 Antibody

IGFBP-2 Antibody

IGFBP-2 Antibody

IGFBP-2 Antibody

IGFBP-2 Antibody

IGFBP-2 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (19)

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IGFBP-2 Antibody from R&D Systems, a Bio-Techne Brand

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