TNF-alpha Antibody AF-510-NA from R&D Systems, a Bio-Techne Brand

Product Specs
ItemTNF-alpha Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF-510-NA
NCBI Gene AliasesDIF, RATTNF, TNFA, TNFSF2, TNF-alpha, TNLG1F, Tnfa
ApplicationsImmunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Neutralization,Immunocytochemistry
Conjugate/TagUnconjugated
ReactivityRat,Transgenic Rat
HostGoat
Quantity100 ug (also 25 ug)
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
TargetTNF-alpha
NCBI Full Gene Nametumor necrosis factor
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant rat TNF-alpha, Leu80-Leu235, Accession # P16599
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects rat TNF-alpha in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant porcine TNF-alpha is observed and less than 1% cross-reactivity with recombinant human (rh) TNF¿ß, rhTNF sRI, and rhTNF sRII is observed. In direct ELISAs, approximately 40% cross-reactivity with recombinant canine TNF-alpha, recombinant feline TNF-alpha, and recombinant rhesus macaque TNF-alpha is observed. Neutralizes the biological activity of rat TNF-alpha. This antibody will also neutralize the biological activity of murine TNF-alpha, but weak neutralization of rhTNF-alpha and no neutralization of rhTNF¿ß was observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Hyperammonemia increases the release of pathological extracellular vesicles from monocytes by impairing lysosomal function and autophagy through the TNFα–cAMP–PKA–LC3 pathwayFrontiers in ImmunologyJanuary 12, 2026Maria A. Pedrosa, Paula Izquierdo-Altarejos, Marta Llansola, Vicente Felipomonocytes from hyperammonemic rats were incubated with an anti-TNFα antibody (aTNFα, 1µg/mL; R&D Systems, #AF-510-NA) or 10 µM of H-89 (Sigma, #371963), a PKA inhibitor (PKAi). Monocytes from control rats were incubated

(2) Extracellular vesicles from mesenchymal stem cells improve neuroinflammation and neurotransmission in hippocampus and cognitive impairment in rats with mild liver damage and minimal hepatic encephalopathyStem cell research & therapyDecember 18, 2024Mincheva G, Moreno-Manzano V, Felipo V, Llansola M.were loaded in 10 or 15% SDS gels. Immunoblots were performed with antibodies against: TNF-α (1:500, AF-510-NA) and IL-1β (1:250, AF-501-NA) from R&D SYSTEMS (Minneapolis, MN, USA); GAD65 (1:1000, ab26113) and GAD67

(3) Extracellular vesicles from mesenchymal stem cells alter gut microbiota and improve neuroinflammation and motor impairment in rats with mild liver damageNeurotherapeutics : the journal of the American Society for Experimental NeuroTherapeuticsOctober 1, 2024Mincheva G, Felipo V, Moreno-Manzano V, Benítez-Páez A, Llansola M.visualization of coloured bands, at room temperature. The following antibodies were used: anti-TNFα (RD Systems AF-510-NA, 1:250), anti-glutaminase I (Novus NBP-1 76544, 1:1000), anti-GAT3 (Abcam ab431, 1:500), anti-BDNF (Invitrogen

(4) Hepatocyte growth factor-modified hair follicle stem cells ameliorate cerebral ischemia/reperfusion injury in ratsStem cell research & therapyFebruary 13, 2023Tang H, Zhang X, Hao X, Dou H, Zou C, Zhou Y, Li B, Yue H, Wang D, Wang Y, et al.(ZO-1, 1:50 dilution; sc-33725, Santa Cruz Biotechnology, Santa Cruz, CA, USA), TNF-α (1:2000 dilution; AF-510-NA, R&D systems, Minneapolis, MN, USA), and IL-4 (1:2000 dilution; AF-504-NA, R&D systems, Minneapolis,

(5) Microglia activation and inflammation in hippocampus attenuates memory and mood functions during experimentally induced diabetes in ratJournal of chemical neuroanatomyNovember 1, 2022Nagayach A, Bhaskar R, Patro I.

(6) Neuroinflammation in the anterior cingulate cortex: the potential supraspinal mechanism underlying the mirror-image pain following motor fiber injuryJournal of neuroinflammationJune 20, 2022Li QY, Chen SX, Liu JY, Yao PW, Duan YW, Li YY, Zang Y.mm, ML 0.5 mm, DV-2.5 mm. After a week of recovery from catheterization, anti-rat CX3CL1 antibody (AF-510-NA, R&D systems, Inc.) or normal goat IgG control (AB-108-C, R&D systems, Inc.) were injected into the

(7) Multiple therapeutic effects of human neural stem cells derived from induced pluripotent stem cells in a rat model of post-traumatic syringomyeliaEBioMedicineMarch 1, 2022Xu T, Li X, Guo Y, Uhlin E, Holmberg L, Mitra S, Winn D, Falk A, Sundström E.ab66047, 1:500), AQP 4 (Sigma, HPA014784, 1:500),IL1β (R&D systems, AF-501-NA,1:20),TNFα (R&D systems, AF-510-NA,1:20),IL1RA (gift from Dr. Stefan Svensson lab, rabbit, 1:100). After incubation, sections were washed

(8) Angiotensin-(1-7) ameliorates high glucose-induced vascular endothelial injury through suppressing chloride channel 3BioengineeredFebruary 1, 2022Cheng F, Liu J, Guo Z, Li S, Chen J, Tu C, Fu F, Shen B, Zhang X, Lai G, et al.dilution) (#8242S, Cell Signaling Technology, Shanghai, China), anti-TNFα antibody (1:1000 dilution) (#AF-510-NA, R&D Systems, Minneapolis, MN, USA), anti-eNOS antibody (1:1000 dilution) (#ab76198, Abcam), anti-CIC-3

(9) Neuronal ApoE4 stimulates C/EBPβ activation, promoting Alzheimer's disease pathology in a mouse modelProg NeurobiolFebruary 1, 2022Wang ZH, Xia Y, Wu Z, Kang SS, Zhang JC, Liu P, Liu X, Song W, Huin V, Dhaenens CM, et al.

(10) ApoE4 activates C/EBPβ/δ-secretase with 27-hydroxycholesterol, driving the pathogenesis of Alzheimer's diseaseProg NeurobiolJuly 1, 2021Wang ZH, Xia Y, Liu P, Liu X, Edgington-Mitchell L, Lei K, Yu SP, Wang XC, Ye K.

(11) The Dual Role of the GABA(A) Receptor in Peripheral Inflammation and Neuroinflammation: A Study in Hyperammonemic RatsInternational journal of molecular sciencesJune 24, 2021Malaguarnera M, Balzano T, Castro MC, Llansola M, Felipo V.cerebellum were subjected to immunoblotting. Primary antibodies were against IL-1β (1:500 dilution (AF-510-NA) from R&D SYSTEMS, Minneapolis, MN, USA), TNFα (1: 500, R&D SYSTEMS), and YM-1 (1:500, Abcam). Secondary

(12) P2X(3) receptor upregulation in trigeminal ganglion neurons through TNFα production in macrophages contributes to trigeminal neuropathic pain in ratsThe journal of headache and painApril 26, 2021Koizumi M, Asano S, Furukawa A, Hayashi Y, Hitomi S, Shibuta I, Hayashi K, Kato F, Iwata K, Shinoda M.(1:250; Wako, Cat#019-19741, RRID:AB_839504, Osaka, Japan), anti-TNFα goat antibody (1:100; R&D systems, Cat#AF510-NA, RRID:AB_354511, Minneapolis, MN, USA), antitumor necrosis factor receptor-2 (TNFR2) mouse antibody (1:50;

(13) Microglia activated by microbial neuraminidase contributes to ependymal cell deathFluids and barriers of the CNSMarch 23, 2021Fernández-Arjona MDM, León-Rodríguez A, López-Ávalos MD, Grondona JM.(rat IL-1β/IL-F2 Antibody, R.D. Systems, AF-501-NA) and anti-TNFα (rat TNFα Antibody, R. D. Systems, AF-510-NA) were added to the culture medium at 5 μg/mL and 2.5 μg/mL respectively; ependymocytes viability was

(14) Mitochondrial pyruvate carrier as a key regulator of fever and neuroinflammationBrain, behavior, and immunityFebruary 1, 2021Guimarães NC, Alves DS, Vilela WR, de-Souza-Ferreira E, Gomes BRB, Ott D, Murgott J, E N de Souza P, de Sousa MV, Galina A, et al.

(15) TNF-α-mediated reduction in inhibitory neurotransmission precedes sporadic Alzheimer's disease pathology in young Trem2^R47H ratsThe Journal of biological chemistryJanuary 1, 2021Ren S, Breuillaud L, Yao W, Yin T, Norris KA, Zehntner SP, D'Adamio L.using Mini Analysis Program. Antibody treatment Right after slice-cutting, 10 ng/ml goat anti–TNF-α (AF-510-NA, R&D Systems) or goat IgG control (AB-108-C, R&D Systems) was incubated and perfused throughout recordings.

(16) TNF-α-mediated reduction in inhibitory neurotransmission precedes sporadic Alzheimer's disease pathology in young Trem2^R47H ratsThe Journal of biological chemistryJanuary 1, 2021Ren S, Breuillaud L, Yao W, Yin T, Norris KA, Zehntner SP, D'Adamio L.using Mini Analysis Program. Antibody treatment Right after slice-cutting, 10 ng/ml goat anti–TNF-α (AF-510-NA, R&D Systems) or goat IgG control (AB-108-C, R&D Systems) was incubated and perfused throughout recordings.

(17) Elamipretide (SS-31) Improves Functional Connectivity in Hippocampus and Other Related Regions Following Prolonged Neuroinflammation Induced by Lipopolysaccharide in Aged RatsFrontiers in aging neuroscienceJanuary 1, 2021Liu Y, Fu H, Wu Y, Nie B, Liu F, Wang T, Xiao W, Yang S, Kan M, Fan L.R&D Systems, Inc., Minneapolis, MN, USA), goat anti-tumor necrosis factor alpha (anti-TNFα) IgG (1:100, AF-510-NA, R&D Systems, Inc., Minneapolis, MN, USA), and mouse anti-glial fibrillary acidic protein (anti-GFAP)-IgG

(18) Circular RNA VMA21 ameliorates sepsis-associated acute kidney injury by regulating miR-9-3p/SMG1/inflammation axis and oxidative stressJournal of cellular and molecular medicineOctober 1, 2020Shi Y, Sun CF, Ge WH, Du YP, Hu NB.

(19) Microglia TREM2(R47H) Alzheimer-linked variant enhances excitatory transmission and reduces LTP via increased TNF-α levelseLifeJune 24, 2020Ren S, Yao W, Tambini MD, Yin T, Norris KA, D'Adamio L.PCR Thermo Rn01511908_g1 Used following manufacturer’s recommendations Antibody Polyclonal Goat IgG anti-Rat TNFa Cat# AF-510-NA R and D Systems RRID:AB_354511 10 ng/ml in ACSF Antibody Polyclonal Goat IgG. antibody Cat# AB-108-C R and D Systems

(20) Effect of amniotic fluid stem cell transplantation on the recovery of bladder dysfunction in spinal cord-injured ratsScientific reportsJune 22, 2020Liang CC, Shaw SS, Ko YS, Huang YH, Lee TH.

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TNF-alpha Antibody

TNF-alpha Antibody

TNF-alpha Antibody

TNF-alpha Antibody

TNF-alpha Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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TNF-alpha Antibody from R&D Systems, a Bio-Techne Brand

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