CCL2/JE/MCP-1 Antibody AF-479-NA from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCCL2/JE/MCP-1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF-479-NA
NCBI Gene AliasesMCP1, Scya2, MCAF, Sigje, HSMCR30, JE, HC11, SMC-CF, GDCF-2, SCYA2, MCP-1
ApplicationsBioassay,ELISA Development,ELISA Development (Capture),Luminex Development,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Dot Blot,In vivo assay,Neutralization,ELISA Capture (Matched Antibody Pair),Immunocytochemistry
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Conjugate/TagUnconjugated
IsotypeIgG
Quantity100 ug (also 25 ug)
ReactivityHuman,Mouse,Rat,Transgenic Mouse
TargetCCL2/JE/MCP-1
HostGoat
NCBI Full Gene NameC-C motif chemokine ligand 2, chemokine (C-C motif) ligand 2
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant mouse CCL2/JE/MCP-1, Gln24-Arg96, Accession # Q5SVU3
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse CCL2/JE/MCP-1 in ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) The CCL2-CCR2 axis drives neuromuscular denervation in amyotrophic lateral sclerosisNature communicationsAugust 1, 2025Nógrádi B, Molnár K, Kristóf R, Horváth O, Huang YT, Ridgway Z, Elicegui A, Fuertes-Alvarez S, Alonso-Martin S, Szebeni GJ, et al.mice received two intramuscular injections (at P7 and P14) of either CCL2 neutralising antibody [Cat# AF-479-NA, RRID: AB_354500, R&D Systems, Minneapolis, MN, USA; 0.4 mg/kg body weight, at the concentration of

(2) Prolactin promotes transepithelial migration of lymphocytes through CCL2Poultry ScienceAugust 1, 2025Peng Liu, Jinjiao Zuo, Hui Lu, Bin Zhang, Caihong Wung/mL prolactin (1445-PR, R&D Systems) and 5 μg/mL CCL2 neutralizing antibodies (goat anti-mouse CCL2, AF-479-NA, R&D Systems) to block CCL2 (Ling et al., 2019). A second group only received 50 ng/mL prolactin. The

(3) IL-23 promotes neuronal ferroptosis via IL-23R/STAT3 signaling after traumatic brain injuryCell Commun SignalJuly 1, 2025Bo Chen, Guihong Shi, Jianye Xu, Xu Zhang, Yanlin Zhu, Lei Li, Cong Wang, Dilmurat Gheyret, Jinchao Wang, Xilei Liu, et al.USA), anti-NeuN antibody ([EPR12763], 1:200, ab177487, Abcam, UK), and goat anti-CCL2 antibody (1:200, AF-479-NA, R&D Systems, USA). Secondary staining was performed using Alexa Fluor antibodies (1:500, A32732/A32723,

(4) Epigenetic erosion of H4K20me1 induced by inflammation drives aged stem cell ferroptosisNature AgingJune 30, 2025Roméo S. Blanc, Nidhi Shah, Sarah Hachmer, Noah A. S. Salama, Fanju W. Meng, Alireza Mousaei, Gayatri Puri, Jeonghye Hannah Hwang, Elizabeth E. Wacker, Benjamin A. Yang, et al.following antibodies were used as previously validated neutralizing antibodies: anti-Ccl2 (R&D Systems, AF479NA); anti-Ccl7 (R&D Systems, AF456NA); and anti-Ccl8 (R&D Systems, MAB281). Neutralizing antibodies were

(5) CCL2-mediated endothelial injury drives cardiac dysfunction in long COVIDNature Cardiovascular ResearchOctober 14, 2024Dilip Thomas, Chikage Noishiki, Sadhana Gaddam, David Wu, Amit Manhas, Yu Liu, Dipti Tripathi, Nimish Kathale, Shaunak S. Adkar, Jaishree Garhyan, et al.dilutions: human CCL2 antibody (R&D systems, AF-279-NA, 1:1000), mouse CCL2 antibody (R&D systems, AF-479-NA, 1:1000), human or mouse pRyR2 antibody (Thermo Fisher Scientific, PA5-36758, 1:500), RyR2 antibody

(6) Matrix stiffness regulates the triad communication of adipocytes/macrophages/endothelial cells through CXCL13Journal of Lipid ResearchSeptember 1, 2024Arthur Choisez, Seiichiro Ishihara, Takuro Ishii, Yidan Xu, Sepideh D. Firouzjah, Hisashi Haga, Ryoichi Nagatomi, Joji KusuyamaELISA Kit (MCX130, R&D systems). Cytokine neutralization was performed by mouse CCL2/JE/MCP-1 antibody (AF-479-NA, R&D systems), mouse CXCL13/BLC/BCA-1 antibody (AF340, R&D systems), mouse/rat IFN-gamma antibody (AF-585-NA,

(7) Hdac3 deficiency limits periosteal reaction associated with Western diet feeding in female miceJournal of cellular and molecular medicineSeptember 1, 2024Vu EK, Karkache IY, Pham A, Koroth J, Bradley EW.and stained with Alizarin Red on day 10. For blocking experiments, the CCL2 neutralizing antibody (AF‐479‐NA, R&D Systems) was used at a concentration of 1 μg/mL. 2.7 Wound healing assays ST2 osteoprogenitor

(8) Localized, highly efficient secretion of signaling proteins by migrasomesCell researchAugust 1, 2024Jiao H, Li X, Li Y, Guo Y, Hu X, Sho T, Luo Y, Wang J, Cao H, Du W, et al.Anti-GAPDH (#60004-1-Ig) and anti-VAMP7 (#22268-1-AP) antibodies were from Proteintech Group. Anti-CCL2 (#AF-479-NA) antibody was from R&D SYSTEMS. Anti-syntaxin4 (#MA5-38156) antibody was from Thermo Fisher Scientific.

(9) Interleukin-10 signaling in somatosensory neurons controls CCL2 release and inflammatory responseBrain, behavior, and immunityFebruary 1, 2024de Souza S, Rosario Claudio J, Sim J, Inyang KE, Dagenais A, Monahan K, Lee B, Ramakrishnan H, Parmar V, Geron M, et al.

(10) B cells mediate lung ischemia/reperfusion injury by recruiting classical monocytes via synergistic B cell receptor/TLR4 signalingJ Clin InvestJanuary 23, 2024Khashayar Farahnak, Yun Zhu Bai, Yuhei Yokoyama, Deniz B. Morkan, Zhiyi Liu, Junedh M. Amrute, Alejandro De Filippis Falcon, Yuriko Terada, Fuyi Liao, Wenjun Li, et al.mice were treated with 25 ng anti-CCL7 antibody (AF-456-NA, R&D Systems) or 25 ng anti-CCL2 antibody (AF-479-NA, R&D Systems) i.v. 24 hours prior to hilar clamp. For B cell depletion, anti-mouse CD20 antibody (clone

(11) B cells mediate lung ischemia/reperfusion injury by recruiting classical monocytes via synergistic B cell receptor/TLR4 signalingJ Clin InvestJanuary 23, 2024Khashayar Farahnak, Yun Zhu Bai, Yuhei Yokoyama, Deniz B. Morkan, Zhiyi Liu, Junedh M. Amrute, Alejandro De Filippis Falcon, Yuriko Terada, Fuyi Liao, Wenjun Li, et al.mice were treated with 25 ng anti-CCL7 antibody (AF-456-NA, R&D Systems) or 25 ng anti-CCL2 antibody (AF-479-NA, R&D Systems) i.v. 24 hours prior to hilar clamp. For B cell depletion, anti-mouse CD20 antibody (clone

(12) Nanoparticles of Lactiplantibacillus plantarum K8 Reduce Staphylococcus aureus Respiratory Infection and Tumor Necrosis Factor Alpha- and Interferon Gamma-Induced Lung InflammationNutrientsNovember 9, 2023Hong J, Son M, Sin J, Kim H, Chung DK.

(13) Astrocytic Slc4a4 regulates blood-brain barrier integrity in healthy and stroke brains via a NO-CCL2-CCR2 pathwaybioRxiv : the preprint server for biologyApril 3, 2023Ye Q, Jo J, Wang CY, Oh H, Choy TJ, Kim K, Dâ Alessandro A, Reshetnyak YK, Jung SY, Chen Z, et al.

(14) Essential roles of the cytokine oncostatin M in crosstalk between muscle fibers and immune cells in skeletal muscle after aerobic exerciseThe Journal of biological chemistryDecember 1, 2022Komori T, Morikawa Y.antibody (diluted at 1:1000, R&D Systems); goat anti-CCL2 antibody (diluted at 1:1000, catalog no.: AF-479-NA, R&D Systems); goat anti-CCL7 antibody (diluted at 1:1000, catalog no.: AF-456-NA, R&D Systems); goat

(15) Inflammatory Role of Milk Fat Globule-Epidermal Growth Factor VIII in Age-Associated Arterial RemodelingJournal of the American Heart AssociationSeptember 6, 2022Ni L, Liu L, Zhu W, Telljohann R, Zhang J, Monticone RE, McGraw KR, Liu C, Morrell CH, Garrido-Gil P, et al.

(16) Infiltration of peripheral immune cells into the olfactory bulb in a mouse model of acute nasal inflammationJournal of neuroimmunologyJuly 15, 2022Asano H, Hasegawa-Ishii S, Arae K, Obara A, Laumet G, Dantzer R, Shimada A.(T) TMEM119,rabbit,Abcam,1:300,ab209064,〇 (C) IL-1β,goat,Abcam,1:200,ab9722,× CCL2,goat,R&D Systems,1:200,AF-479-NA,× CXCL1,goat,R&D Systems,1:100,AF-453-NA,× E-Selectin,chicken,R&D Systems,1:100,AF575,〇 (T) VCAM-1,rabbit,Abcam,1:500,ab134047,〇

(17) MCP-1 targeting: Shutting off an engine for tumor developmentOncology lettersJanuary 1, 2022Wang L, Lan J, Tang J, Luo N.human MCP-1 (85) 5D3-F7,Recombinant human MCP-1 antibody,BD Biosciences,1994,Human sarcoma (86), AF-479-NA,Mouse MCP-1 antibody,R&D systems,2000,Human gastric cancer (87); breast cancer (88),Cross-reacted with

(18) Reticular Fibroblasts Expressing the Transcription Factor WT1 Define a Stromal Niche that Maintains and Replenishes Splenic Red Pulp MacrophagesImmunityJuly 14, 2020Bellomo A, Mondor I, Spinelli L, Lagueyrie M, Stewart BJ, Brouilly N, Malissen B, Clatworthy MR, Bajénoff M.(RA3-6B2) ebioscience Cat#13-0452-85; RRID: AB_466450 Anti-mouse CCL2 (Polyclonal Goat IgG) R&D Systems Cat#AF-479-NA; RRID: AB_354500 Anti-mouse CCL7 (Polyclonal Goat IgG) R&D Systems Cat#AF-456-NA; RRID: AB_2071565 Anti-mouse

(19) Inhibiting NF-κB Signaling Activation Reduces Retinal Neovascularization by Promoting a Polarization Shift in MacrophagesInvestigative ophthalmology & visual scienceJune 3, 2020Sui A, Chen X, Demetriades AM, Shen J, Cai Y, Yao Y, Yao Y, Zhu Y, Shen X, Xie B.

(20) IgE Contributes to Atherosclerosis and Obesity by Affecting Macrophage Polarization, Macrophage Protein Network, and Foam Cell FormationArteriosclerosis, thrombosis, and vascular biologyMarch 1, 2020Zhang X, Li J, Luo S, Wang M, Huang Q, Deng Z, de Febbo C, Daoui A, Liew PX, Sukhova GK, et al.14-0081-85; eBiosciences, San Diego, CA), and MCP-1 (monocyte chemoattractant protein 1, 1:100, no. AF-479-NA, R&D Systems, Minneapolis, MN) antibodies, followed by appropriate biotin-conjugated secondary antibodies

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CCL2/JE/MCP-1 Antibody

CCL2/JE/MCP-1 Antibody

CCL2/JE/MCP-1 Antibody

CCL2/JE/MCP-1 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (72)

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CCL2/JE/MCP-1 Antibody from R&D Systems, a Bio-Techne Brand

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