Human FGF basic/FGF2/bFGF Antibody AF-233-NA from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman FGF basic/FGF2/bFGF Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $539.00Supplier Page View Company Product Page
Catalog NumberAF-233-NA
ApplicationsBioassay,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Dot Blot,In vivo assay,Neutralization,Immunocytochemistry,ELISA Capture
TargetFGF basic/FGF2/bFGF
IsotypeIgG
HostGoat
Quantity100 ug (also 25 ug)
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityAvian,Bovine,Hamster,Human,Mouse,Rat
Conjugate/TagUnconjugated
ClonalityPolyclonal
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namefibroblast growth factor 2
NCBI Gene AliasesBFGF, FGF-2, FGFB, HBGF-2
ImmunogenBovine brain-derived FGF basic/FGF2/bFGF

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this antibody effectively neutralize FGF2 activity in vivo?

Yes, the FGF basic/FGF2/bFGF antibody (AF-233-NA) has been successfully used to block FGF2 activity in vivo, as demonstrated in studies where it was utilized to inhibit the proliferation of neonatal oligodendrocyte lineage cells in response to FGF2 treatment ().

What applications has this antibody been shown to work in?

The FGF basic/FGF2/bFGF antibody has been shown to work in various applications including Western blotting, ELISA, immunocytochemistry, and immunohistochemistry, as well as in functional assays related to cell proliferation and differentiation ().

Is this antibody specific for human FGF2, and does it cross-react with other species?

The antibody is specific for human and bovine FGF basic/FGF2/bFGF, with approximately 75% cross-reactivity observed with recombinant mouse FGF basic/FGF2/bFGF in direct ELISAs and Western blots ().

How has this antibody been utilized in cancer research?

In cancer research, the FGF basic/FGF2/bFGF antibody has been used to investigate the role of FGF2 in tumor microenvironments, particularly in studies examining how FGF2 contributes to tumor-associated fibroblast activity and therapy resistance in melanoma ().

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Exploring the wound healing potential of dietary nitrate in diabetic rat modelFrontiers in PhysiologyNovember 20, 2024Xiaodan Hu, Haoyue Xu, Lingxue Bu, Jian Sun, Jiangzhi Deng, Kai Song, Lin Wang, Baoxing Pangimmunofluorescent staining for CD31 (Abcam, AB28364), VEGF (R&D Systems, AF564), bFGF (R&D Systems, AF-233-NA), α-SMA (Abcam, AB32575), IL-6 (Affinity, DF6087), IL-1β (Affinity, AF5103), TNF-α (Affinity, AF7014)

(2) Small Extracellular Vesicle-Derived vWF Induces a Positive Feedback Loop between Tumor and Endothelial Cells to Promote Angiogenesis and Metastasis in Hepatocellular CarcinomaAdvanced science (Weinheim, Baden-Wurttemberg, Germany)September 1, 2023Wong SWK, Tey SK, Mao X, Fung HL, Xiao ZJ, Wong DKH, Mak LY, Yuen MF, Ng IO, Yun JP, et al.

(3) XBP1-elicited environment by chemotherapy potentiates repopulation of tongue cancer cells by enhancing miR-22/lncRNA/KAT6B-dependent NF-κB signallingClinical and translational medicineJanuary 1, 2023Jia X, Wang G, Wu L, Pan H, Ling L, Zhang J, Wen Q, Cui J, He Z, Qi B, et al.antibodies (Abs) against amphiregulin (AREG) (AB‐262‐NA) and basic fibroblast growth factor (bFGF) (AF‐233‐NA) were obtained from R&D Systems (Minneapolis, MN, USA). 2.2 Cells and cell culture The human tongue cancer

(4) Fibroblast growth factor-2 bound to specific dermal fibroblast-derived extracellular vesicles is protected from degradationScientific reportsDecember 22, 2022Petit I, Levy A, Estrach S, Féral CC, Trentin AG, Dingli F, Loew D, Qu J, Zhou H, Théry C, et al.Sigma), syntenin-1 (#133267, 1:2000, Abcam), TSG101 (Clone 51/TSG101, 1:1000, BD Biosciences), FGF2 (AF-233-NA, 0.1 μg/ml, R&D Systems) phosphorylated forms and total forms of AKT (#4060, 1:1000 and #9272, 1:2000,

(5) Heme stimulates platelet mitochondrial oxidant production to induce targeted granule secretionRedox biologyDecember 1, 2021Annarapu GK, Nolfi-Donegan D, Reynolds M, Wang Y, Kohut L, Zuckerbraun B, Shiva S.

(6) Three subtypes of lung cancer fibroblasts define distinct therapeutic paradigmsCancer cellNovember 8, 2021Hu H, Piotrowska Z, Hare PJ, Chen H, Mulvey HE, Mayfield A, Noeen S, Kattermann K, Greenberg M, Williams A, et al.A-31571 anti-FGF1 neutralizing antibody,R&D System,Cat# AF232 anti-FGF2 neutralizing antibody,R&D System,Cat# AF-233-NA anti-FGF5 neutralizing antibody,R&D System,Cat# AF-237-NA anti-FGF7 neutralizing antibody,R&D System,Cat#

(7) A digital single-molecule nanopillar SERS platform for predicting and monitoring immune toxicities in immunotherapyNature communicationsFebruary 17, 2021Li J, Wuethrich A, Sina AAI, Cheng HH, Wang Y, Behren A, Mainwaring PN, Trau M.

(8) Inhibition of protein glycosylation is a novel pro-angiogenic strategy that acts via activation of stress pathwaysNature communicationsDecember 10, 2020Zhong C, Li P, Argade S, Liu L, Chilla' A, Liang W, Xin H, Eliceiri B, Choudhury B, Ferrara N.anesthetized with ketamine/xylazine cocktail. The indicated amounts of ManN, Kif, or bFGF (R&D Systems, AF-233-NA) in 1 μl of PBS and PBS vehicle control were injected intravitreally with a 33-gauge Hamilton syringe.

(9) Metastatic Brain Tumors Disrupt the Blood-Brain Barrier and Alter Lipid Metabolism by Inhibiting Expression of the Endothelial Cell Fatty Acid Transporter Mfsd2aScientific reportsMay 29, 2018Tiwary S, Morales JE, Kwiatkowski SC, Lang FF, Rao G, McCarty JH.

(10) Multicolor quantitative confocal imaging cytometryNature methodsJanuary 1, 2018Coutu DL, Kokkaliaris KD, Kunz L, Schroeder T.

(11) Tumor-associated B-cells induce tumor heterogeneity and therapy resistanceNature communicationsSeptember 19, 2017Somasundaram R, Zhang G, Fukunaga-Kalabis M, Perego M, Krepler C, Xu X, Wagner C, Hristova D, Zhang J, Tian T, et al.(LS-C7504; Seattle, WA); goat anti-human FGF-2 neutralizing antibody was obtained from R&D Systems (AF-233-NA; Minneapolis, MN); rabbit anti-human FGFR-3 (ab10651), phospho-FGFR-3 (ab155960) and IGF-1 (ab9572)

(12) Pericytes impair capillary blood flow and motor function after chronic spinal cord injuryNature medicineJune 1, 2017Li Y, Lucas-Osma AM, Black S, Bandet MV, Stephens MJ, Vavrek R, Sanelli L, Fenrich KK, Di Narzo AF, Dracheva S, et al.

(13) Discovery of pan-VEGF inhibitory peptides directed to the extracellular ligand-binding domains of the VEGF receptorsScience advancesOctober 1, 2016Michaloski JS, Redondo AR, Magalhães LS, Cambui CC, Giordano RJ.anti-human VEGF-C (AF752), anti-human PlGF (AF-264-PB), anti-human PDGF-BB (AF-220-NA), anti-human FGF-basic (AF-233-NA), anti-mouse/rat NRP-1 (AF566), and anti-mouse/rat NRP-2 (AF567) were from R&D Systems; anti-fd Bacteriophage-Biotin

(14) Stearoyl-CoA desaturase 1 and paracrine diffusible signals have a major role in the promotion of breast cancer cell migration induced by cancer-associated fibroblastsBritish journal of cancerMay 12, 2015Angelucci C, Maulucci G, Colabianchi A, Iacopino F, D'Alessio A, Maiorana A, Palmieri V, Papi M, De Spirito M, Di Leone A, et al.

(15) Peroxynitrite upregulates angiogenic factors VEGF-A, BFGF, and HIF-1α in human corneal limbal epithelial cellsInvestigative ophthalmology & visual scienceMarch 19, 2014Ashki N, Chan AM, Qin Y, Wang W, Kiyohara M, Lin L, Braun J, Wadehra M, Gordon LK.

(16) Correction: Mesenchymal Stem Cell Transition to Tumor-Associated Fibroblasts Contributes to Fibrovascular Network Expansion and Tumor ProgressionPLoS ONEMarch 19, 2013Erika L. Spaeth, Jennifer L. Dembinski, A. Kate Sasser, Keri Watson, Ann Klopp, Brett Hall, Michael Andreeff, Frank Mariniwas incubated with 2 mg/mL anti-GF monoclonal antibody (Mab206, Mab636, Mab294, Mab293, Mab1835 and AF233NA; R&D Systems) for 4 to 6 h at 4uC with constant rotation. To verify that each GF had been removed from

(17) Endothelial heparan sulfate 6-O-sulfation levels regulate angiogenic responses of endothelial cells to fibroblast growth factor 2 and vascular endothelial growth factorThe Journal of biological chemistryOctober 19, 2012Ferreras C, Rushton G, Cole CL, Babur M, Telfer BA, van Kuppevelt TH, Gardiner JM, Williams KJ, Jayson GC, Avizienyte E.

(18) Inhibition of PAI-1 induces neutrophil-driven neoangiogenesis and promotes tissue regeneration via production of angiocrine factors in miceBloodJune 28, 2012Tashiro Y, Nishida C, Sato-Kusubata K, Ohki-Koizumi M, Ishihara M, Sato A, Gritli I, Komiyama H, Sato Y, Dan T, et al.

(19) Bone marrow stroma-secreted cytokines protect JAK2(V617F)-mutated cells from the effects of a JAK2 inhibitorCancer researchJune 1, 2011Manshouri T, Estrov Z, Quintás-Cardama A, Burger J, Zhang Y, Livun A, Knez L, Harris D, Creighton CJ, Kantarjian HM, et al.-chemokine C-X-C-motif ligand 10 (CXCL10/IP10, AF-266-NA), and –fibroblast growth factor basic/2 (FGF2, AF233-NA) were obtained from R&D Systems (Minneapolis, MN). Mouse anti-β-actin (A5441) was purchased from Sigma-Aldrich

(20) The fibroblast-derived paracrine factor neuregulin-1 has a novel role in regulating the constitutive color and melanocyte function in human skinJournal of cell scienceSeptember 15, 2010Choi W, Wolber R, Gerwat W, Mann T, Batzer J, Smuda C, Liu H, Kolbe L, Hearing VJ.

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FGF basic/FGF2/bFGF Antibody

FGF basic/FGF2/bFGF Antibody

FGF basic/FGF2/bFGF Antibody

Human FGF basic/FGF2/bFGF Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (34)

Reviews

Human FGF basic/FGF2/bFGF Antibody from R&D Systems, a Bio-Techne Brand

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