Galectin-3 Antibody AF1197 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemGalectin-3 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF1197
NCBI Gene AliasesGALBP, MAC2, GAL3, L-34, GALIG, gal3, Mac-2, CBP35, GBP, L31, LGALS2
ApplicationsIFC,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Simple Western,Immunocytochemistry
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
TargetGalectin-3
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman,Mouse,Rat,Transgenic Mouse,Xenograft
HostGoat
IsotypeIgG
NCBI Full Gene Namelectin, galactose binding, soluble 3, galectin 3
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant mouse Galectin-3, Ala2-Ile264, Accession # NP_034835
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human, mouse, and rat Galectin-3 in Western blots. Detects mouse Galectin-3 in direct ELISAs. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Galectin-3 is observed and less than 1% cross-reactivity with recombinant mouse (rm) Galectin-4 and rmGalectin-9 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this Galectin-3 antibody be used in immunofluorescence applications?

Yes, the Galectin-3 antibody has been shown to work in immunofluorescence applications, as evidenced by its use in studies where it was applied to tissue sections for detecting Galectin-3 in various models, including those related to Alzheimer's disease and Huntington's disease (, ).

What species does this antibody react with, and how has it been utilized in research?

The Galectin-3 antibody is reactive with human, mouse, and rat samples. It has been utilized in various research contexts, including studies on neuroinflammation in Huntington's disease and the role of Galectin-3 in microglial activation (, ).

Is there any evidence of this antibody being used in Western blotting?

Yes, the Galectin-3 antibody has been successfully used in Western blotting, as demonstrated in studies where it was employed to analyze protein expression levels in various experimental models, including those investigating macrophage responses and neurodegenerative diseases (, ).

How does the Galectin-3 antibody contribute to understanding disease mechanisms in models of neurodegeneration?

The Galectin-3 antibody has been used to elucidate mechanisms of inflammation and cellular dysfunction in neurodegenerative diseases. For instance, it has been shown to be upregulated in microglia during Huntington's disease, contributing to inflammatory responses and lysosomal dysfunction, which are critical in understanding disease pathology (, ).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Reduced LACTB expression in myeloid cells is associated with elevated succinylcarnitine and reduced Alzheimer's disease riskResearchSquareJuly 13, 2026Alison Goate, Carmen Romero-Molina, Ruben Gomez-Gutierrez, Wen Yi See, Tulsi Patel, Hayk Davtyan, Jiacheng Ma, Quanyun Xu, Michael Sewell, Kendra Allton, et al.,Cell Signaling,2180S Anti-IBA1 (immunostaining),Abcam,AB5076 ThioS,Sigma-Aldrich,T1892 Anti-LGALS3,R&D Systems,AF1197 Anti-HLA-DRBI (LN3),Invitrogen,14-9956-82 Anti-CD9,Biolegend,312102 "Chemicals, peptides, and recombinant

(2) Anti-amyloid nanobody-Fc fusion protein drives potent amyloid clearance through microglial recruitmentAlzheimers Dement (N Y)July 12, 2026Zonghui Ding, Katelin A. Gibson, David G. Nascari, Lauren E. Tallant, Samantha M. Bouchal, Dennis W. Dickson, John D. Fryer750‐conjugated mouse anti‐human Aβ (1:500, NBP2‐13075AF750; Novus), goat anti‐mouse LGALS3 (1:300, AF1197, R&D Systems), rabbit anti‐IBA1 antibody (1:500, Catalog No.: 019‐19741, FUJIFILM), Alexa Fluor 488‐conjugated

(3) Amyloid exacerbates tau and alpha-synuclein pathologies, behavioral impairments, and neuroinflammation in a mixed dementia modelAlzheimers DementJuly 3, 2026Benjamin E. Rabichow, Lacey Nixon, Lauren E. Tallant, Katelin A. Gibson, David G. Nascari, J. Hudson Barnett, Nashali Massa, John D. FryerNo.: S32357). Glia and plaque stain: goat anti‐galectin‐3 (LGALS3; 1:300; R&D Systems, Catalog No.: AF1197), rabbit anti‐Iba1 (1:500; Wako, Catalog No.:019‐19741), chicken anti‐glial fibrillary acidic protein

(4) Environmental enrichment modulates chronic poststroke inflammation and links white matter TREM2‐positive microglia in recovery in miceNeuroprotectionMarch 1, 2026Lluís Camprubí‐Ferrer, Ömer K. Kolbay, Miriana J. Quattromani, Tadeusz Wieloch, Karsten Ruscher, Tomas Deierborgone of the following antibodies: Iba1 (1:500, rabbit, Cat# 016‐26721; Wako), Gal3 (1:750, goat, Cat# AF1197; R&D Systems) P2RY12 (1:200, rat, Cat# S16007D; Biolegend). Following overnight incubation, the samples

(5) Neutrophil-microglia interaction drives motor dysfunction in a neuromyelitis optica model induced by subarachnoid AQP4-IgGJ Clin InvestFebruary 10, 2026Fangfang Qi, Vanda A. Lennon, Shunyi Zhao, Yong Guo, Husheng Ding, Caiyun Liu, Whitney M. Bartley, Tingjun Chen, Claudia F. Lucchinetti, Long-Jun Wusmall-cell lung carcinoma; 1:10,000), polyclonal goat anti-human/mouse/rat Galectin-3 antibody (R&D Systems; AF1197, NP_034835, 1: 1,000), and mouse anti-4-HNE (R&D Systems; MAB3249-SP, clone 198960, 1:200). Sections

(6) Neutrophil-microglia interaction drives motor dysfunction in a neuromyelitis optica model induced by subarachnoid AQP4-IgGJournal of Clinical InvestigationFebruary 10, 2026Fangfang Qi, Vanda A. Lennon, Shunyi Zhao, Yong Guo, Husheng Ding, Caiyun Liu, Whitney M. Bartley, Tingjun Chen, Claudia F. Lucchinetti, Long-Jun Wusmall-cell lung carcinoma; 1:10,000), polyclonal goat anti-human/mouse/rat Galectin-3 antibody (R&D Systems; AF1197, NP_034835, 1: 1,000), and mouse anti-4-HNE (R&D Systems; MAB3249-SP, clone 198960, 1:200). Sections

(7) Altered distribution of tissue galectins correlates with mucosal dysregulation in SIV infectionThe Journal of ImmunologyJanuary 21, 2026Thomas A Premeaux, Stephen T Yeung, Samuel D Johnson, Preeti Moar, Siddappa N Byrareddy, Lishomwa C Ndhlovuantibodies include those targeting human galectin-9 (Cat. AF2045; R&D Systems) and galectin-3 (Cat. AF1197; R&D Systems), galectin-1 (Cat. Ab108389; Abcam), and ZO-1 (Cat. 61-7300; ThermoFisher), Occludin (Cat.

(8) Selective deletion of Slc2a1 from the RPE reveals that rods but not cones depend on glucose transport across the outer blood-retinal barrieriScienceJanuary 16, 2026Lauren L. Daniele, John Y.S. Han, Minzhong Yu, Ravi A. Sangani, Craig D. Beight, Cyrus Rostami, Philip D. Kiser, Neal S. Peachey, Nancy J. Philparrestin (Arr3),EMD/Millipore,Cat#ab15282; RRID: AB_1163387 Goat anti - galectin - 3,R & D Systems,Cat#AF1197; RRID: AB_2234687 Rabbit anti - GFAP,Sigma,Cat#SAB4300647; RRID: AB_11128424 Rabbit anti - Glut1,Alpha

(9) Galectin-3 shapes microglial phenotype through endogenous and exogenous mechanismsFront Cell NeurosciDecember 18, 2025Lluís Camprubí-Ferrer, Yiyi Yang, Rosalía Fernández-Calle, Antonio Boza-Serrano, Juan García-Revilla, Javier Frontiñán-Rubio, Tomas Deierborgdescribed above. Primary antibodies used were: anti-galectin-3 (1:1000 dilution, goat, R&D Systems, Cat: AF1197), anti-TREM-2 (1:750 dilution, sheep, R&D Systems, Cat: AF1729), anti-TLR4 (1:1000 dilution, rabbit,

(10) Noradrenergic signaling controls Alzheimer’s disease pathology via activation of microglial β2 adrenergic receptorsBrain, Behavior, and ImmunityAugust 1, 2025L.H.D. Le, A.M. Feidler, Rodriguez L. Rodriguez, M. Cealie, E. Plunk, H. Li, K. Kara-Pabani, C. Lamantia, M.K. O’Banion, A.K. MajewskaMillipore Sigma), rat anti-LAMP1 (14-1071-82, clone 1D4B, 1:2000, Invitrogen), goat anti-Galectin3 (AF1197, 1:1500, R&D Systems), sheep anti-TREM2 (AF1729, 1:1000, R&D Systems). Sections were incubated in primary

(11) TFEB overexpression alleviates autophagy-lysosomal deficits caused by progranulin insufficiencyScientific reportsJuly 19, 2025Nader WO, Brown KS, Boyle NR, Kaplelach AK, Abdelaziz SM, Davis SE, Aljabi Q, Hakim AR, Davidson AG, Vollmer GA, et al.(Cell Signaling Technologies #3724), SCMAS (Abcam #ab181243), CD68 (Bio-Rad #MCA1957), or Galectin 3 (R&D Systems #AF1197). The following day, sections were incubated with biotinylated species-matched secondary antibodies

(12) Organ of Corti macrophages: a distinct group of cochlear macrophages with potential roles in supporting cell degeneration and survivalFrontiers in ImmunologyJune 25, 2025Mengxiao Ye, Celia Zhang, Dalian Ding, Guang-Di Chen, Henry J. Adler, Rania Sharaf, Bo Hua HuAntibody,Macrophages,MCA1957GA,Bio-Rad Laboratories Galectin-3 Antibody,Macrophages and Hensen’s cells,AF1197,Novus Biologicals IFIT3 Antibody,Deiters’ cells and pillar cells,PA522230,Invitrogen Myosin 7α Antibody,Hair

(13) Direct microglia replacement reveals pathologic and therapeutic contributions of brain macrophages to a monogenic neurological diseaseImmunityMay 13, 2025William H. Aisenberg, Carleigh A. O’Brien, Madison Sangster, Fazeela Yaqoob, Yuanchao Zhang, Brian Temsamrit, Searlait Thom, Luca Gosse, Sai Chaluvadi, Bilal Elfayomi, et al.anti-mouse Pdgfr-a,R&D systems,"Cat# AF1062, RRID:AB_2236897" Goat anti-mouse Gal3,R&D systems,"Cat# AF1197, RRID:AB_2234687" Goat anti-GPNMB,R&D systems,"Cat# AF2330, RRID:AB_2112934" Rabbit anti-mouse Fibronectin,Sigma,"Cat#

(14) Proteostasis and lysosomal repair deficits in transdifferentiated neurons of Alzheimer's diseaseNature cell biologyApril 1, 2025Chou CC, Vest R, Prado MA, Wilson-Grady J, Paulo JA, Shibuya Y, Moran-Losada P, Lee TT, Luo J, Gygi SP, et al.ADI-905-804-100), anti-CHMP2B (1:100; Proteintech, 12527-1-AP), anti-galectin-3 (1:50; R&D systems, AF1197), anti-Hsp70 (1:300; Abcam, ab45133), anti-LAMP1 (1:50; Santa Cruz, sc-19992) and anti-MAP2 (1:500;

(15) Supporting cell involvement in cochlear damage and repair: Novel insights from a quantitative analysis of cyclodextrin-induced ototoxicity in miceHearing ResearchApril 1, 2025Dalian Ding, Guang Di Chen, Celia Zhang, Mengxiao Ye, Henry J. Adler, Rania Sharaf, Kayla Naldrett, Tanisi Mittal, Bo Hua Hunumber,Company IFIT3 Antibody,Deiters’ cells and pillar cells,PA522230,Invitrogen Galectin-3 Antibody,Hensen’s cells,AF1197,Novus Biologicals MYO7A Antibody,Hair cells,PA1-936,ThermoFisher Scientific DAPI,Nuclei,62248,ThermoFisher Scientific

(16) The microglial response to inhibition of Colony-stimulating-factor-1 receptor by PLX3397 differs by sex in adult miceCell ReportsJanuary 28, 2025Linh H.D. Le, Sophia Eliseeva, Elizabeth Plunk, Kallam Kara-Pabani, Herman Li, Felix Yarovinsky, Ania K. Majewskamicroglia (rabbit anti-Iba1, 019–19741, 1:2000, Wako) alone, with Galectin-3 (goat anti-Galectin3, AF1197, 1:1500, R&D Systems), or a combination of pH3 (rabbit anti-pH3, 06–570, 1:500, Millipore) and Galectin-3.

(17) Proteostasis and lysosomal repair deficits in transdifferentiated neurons of Alzheimer's diseasebioRxiv : the preprint server for biologyJanuary 13, 2025Chou CC, Vest R, Prado MA, Wilson-Grady J, Paulo JA, Shibuya Y, Moran-Losada P, Lee TT, Luo J, Gygi SP, et al.ADI-905–804-100), anti-CHMP2B (1:100; Proteintech, 12527–1-AP), anti-Galectin-3 (1:50; R&D systems, AF1197), anti-Hsp70 (1:300; Abcam, ab45133), anti-LAMP1 (1:50; Santa Cruz, sc-19992), and anti-MAP2 (1:500;

(18) Granulins rescue inflammation, lysosome dysfunction, lipofuscin, and neuropathology in a mouse model of progranulin deficiencyCell reportsDecember 24, 2024Root J, Mendsaikhan A, Taylor G, Merino P, Nandy S, Wang M, Araujo LT, Ryu D, Holler C, Thompson BM, et al.polyclonal anti-human granulin 4,This paper,N/A Goat polyclonal anti-mouse galectin-3,R and D Systems,Cat# AF1197; RRID: AB_2234687 Goat polyclonal anti-mouse cathepsin Z,Thermo Fisher Scientific,PA5–47048; RRID:

(19) Loss of Pigment Epithelium Derived Factor Sensitizes C57BL/6J Mice to Light-Induced Retinal DamagebioRxivDecember 7, 2024Debresha A. Shelton, Jack T. Papania, Tatiana E. Getz, Jana T. Sellers, Preston E. Giradot, Micah A. Chrenek, Hans E. Grossniklaus, Jeffrey H. Boatright, John M. NickersonAntibody,Antibody Type,Species,Company and Catalog information,Concentration Galectin-3,Primary,Goat,R&D Systems ( AF1197),1:250 ZO-1,Primary,Rat,Sigma,1:250 Vimentin(D21H3),Primary,Rabbit,Cell Signaling (mAB5741S),1:200 IGF-1,Primary

(20) Farnesylthiosalicylic Acid Through Inhibition of Galectin‐3 Improves Neuroinflammation in Alzheimer Disease via Multiple PathwaysCNS Neuroscience & TherapeuticsNovember 26, 2024Qing Qiu, Cui Li, Xiaoli Zhao, Mengting Yang, Shushu Ding, Haiying Liang, Tingting Chenanti‐Aβ (6E10) (1:1000; BioLegend, Cat# 803014), anti‐Flag (Sigma, Cat# F1804), Gal‐3 (1:1000; Cat# AF1197, Bio‐techne), PS1 (1:1000; Cat# ab76083, Abcam), IDE (1:1000; Cat# ab133561, Abcam), NEP (1:1000; Cat#

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