Mouse PDGF R alpha Antibody AF1062 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse PDGF R alpha Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF1062
NCBI Gene AliasesCD140a, Pdgfr-2
ApplicationsBioassay,IHC frozen fixed,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,In vivo assay,Neutralization,Immunofluorescence,Immunocytochemistry,Immunocytochemistry/ Immunofluorescence
HostGoat
IsotypeIgG
TargetPDGF R alpha
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityGuinea Pig,Human,Mouse,Porcine,Primate - Chlorocebus aethiops (African Green Monkey),Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque),Rat,Transgenic Mouse,Transgenic Rat,Xenograft
NCBI Full Gene Nameplatelet derived growth factor receptor, alpha polypeptide
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse PDGF R alpha, Leu25-Glu524 (Asp65Glu, Gly439Ala, Thr440Ala), Accession # P26618
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

What species does this antibody work with, and has it been used in relevant studies?

The PDGF R alpha antibody has been shown to react with mouse, rat, non-human primate, and human samples. It has been utilized in various studies, including those examining the role of PDGFRα in FACS cell isolation and immunofluorescence staining, as seen in research by Bahar Halpern et al. () and Fernández-Castañeda et al. ().

Can this antibody be used for immunofluorescence applications, and what are some examples of its usage?

Yes, the PDGF R alpha antibody has been successfully used for immunofluorescence applications. For instance, in the study by Fernández-Castañeda et al. (), it was employed at a concentration of 8 µg/µl for immunofluorescence staining, demonstrating its effectiveness in visualizing PDGFRα in tissue samples.

How specific is this antibody in detecting PDGF R alpha, and what are the implications for its use in experiments?

The PDGF R alpha antibody exhibits less than 1% cross-reactivity with recombinant human PDGF R alpha and PDGF R beta, indicating high specificity for mouse PDGF R alpha. This specificity is crucial for experiments aiming to delineate the role of PDGF R alpha in various biological contexts, such as those involving oligodendrocyte differentiation and myelination, as highlighted in studies like Gibson et al. ().

What are some experimental contexts where this antibody has been cited, and what findings were associated with its use?

The PDGF R alpha antibody has been cited in studies investigating the effects of PDGF signaling in various contexts, including muscle regeneration and neural cell differentiation. For example, Wosczyna et al. () demonstrated the necessity of PDGFRα+ fibro-adipogenic progenitors in skeletal muscle regeneration, emphasizing the antibody's role in elucidating the mechanisms of muscle homeostasis and repair.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Directional guidance to orient Schwann cell alignment in nerve regeneration requires Plexin-B1Sci AdvApril 24, 2026Jiaxi Li, Haofei Ni, Dalia Halawani, Molly Estill, Aarthi Ramakrishnan, Chrystian Junqueira Alves, Li Shen, Xijing He, Roland H. Friedel, Hongyan Zouanti-MBP (host: rabbit, Cell Signaling Technology, 78896; 1:50), anti-PDGFRα (host: goat, R&D Systems, AF1062; 1:50), anti-Pecam1/CD31 (host: rat, BD Pharmingen, 553370; 1:250), anti-Reticulin (host: rat, Abcam,

(2) PDGFRα-positive mesenchymal stem/stromal cells contribute to autonomous vascular formation through in-body tissue architecturePLoS OneApril 16, 2026Satoru Morikawa, Masaki Yoda, Takehito Ouchi, Takazumi Yasui, Kazumasa Fukuda, Michiru Sugimoto, Rintaro Harada, Tatsuaki Matsumoto, Yo Mabuchi, Osahiko Tsuji, et al.5% donkey serum in 1% BSA for 1 h. Primary antibody incubation was performed with goat anti-PDGFRα (AF1062, 1: 50; R&D Systems, Minneapolis, MN, USA), rabbit anti-RFP (ab62341, 1: 250; Abcam, Cambridge, UK),

(3) PHGDH-mediated serine synthesis reduces oligodendrocyte death by sustaining GSH and NADPH levels after brain ischemiaFront PharmacolApril 15, 2026Zhaolong Zhang, Jinhua Tan, Mengfei Lv, Zhongying Duan, Congxiao Wang, Mingchen Gao, Yu Cui, Yugong Feng1:500), mouse anti-GFAP (MAB360, Millipore, 1:1000), goat anti-Iba-1 (NB100-1028, Novus, 1:1000), PDGFRα (AF1062, R&D, 1:1000), APC (GTX16794, Gentex, 1:100), MBP (ab40390, abcam, 1:1000), SMI32 (801701, Biologend,

(4) Developmental Profiling of Structural and Functional Maturation in Mouse Corpus CallosumGliaApril 2, 2026Hayes Johnson, Maren Beall, Sophia Latifi, Juan Peng, Wenjing Sunfor immunohistochemistry, Millipore, AB1783, RRID:AB_90949). Goat anti‐PDGFRα (1:400 for immunohistochemistry, R&D Systems, AF1062, RRID: AB_2236897). Mouse anti‐CC1 (1:400 for immunohistochemistry, Calbiochem, OP80‐100UG, RRID:AB_2057371). Goat

(5) Fibro-Adipogenic Progenitors Regulate Orofacial Neuromuscular Junction Regeneration via MyostatinJ Cachexia Sarcopenia MuscleApril 1, 2026Ruizhi Li, Ruojing Liu, Yixuan Huang, Yijue Wang, Xu Cheng, Jingtao Li, Shujuan Zou, Xing Yinantibody (1:20, F5D, DSHB, USA), Pax7 antibody (1:10, PAX7, DSHB, USA) and PDGFRα antibody (1:300, AF1062, R&D Systems, USA). Secondary antibodies include Goat Anti‐Mouse DyLight 488 (1:200, Abbkine, China)

(6) Sex-specific APOE4-dependent innate immunity regulates meningeal lymphatics, brain lipids, neuroinflammation, and cognitionNeuronMarch 26, 2026Nickoleta Delivanoglou, Kennedi T. Todd, Francisco Almeida, Shanon Rego, Amogh Changavi, Myriam Spajer, Virginia Estades Ayuso, Liliana M. Pinho-Correia, Guadalupe Sanchez, Sofia P. das Neves, et al.GFAP,Abcam,Cat#ab4674 "Mouse anti-mouse Quaking 7, clone CC1",Sigma-Aldrich,Cat#OP80 Goat anti-mouse PDGFRα,R&D Systems,Cat#AF1062 Donkey anti-goat AF488,Thermo Fisher Scientific,Cat#A-11055 Donkey anti-rabbit AF488,Thermo Fisher Scientific

(7) Inflammatory Mediators Both Directly and Indirectly Promote Microglial ProliferationGliaMarch 19, 2026Brady P. Hammond, Eugene Hahn, Kelly V. Lee, Bradley J. Kerr, Jason R. Plemelrabbit anti‐βIII tubulin (1 μg/mL; Sigma‐Aldrich T2200), goat anti‐PDGFRɑ (2 μg/mL; Novus Biologicals AF1062), mouse anti‐O4 (1.25 μg/mL; R&D Systems MAB1326), and rabbit anti‐GFAP (3.9 μg/mL; Agilent‐Dako Z0334)

(8) Functional and structural pathologies in skeletal muscle of a rat model of Duchenne muscular dystrophySkeletal MuscleFebruary 25, 2026Young Il Lee, Cora C. Hart, C. Spencer Henley-Beasley, Jeffrey S. Herr, Eli Zerpa, Elisabeth R. Barton, David W. Hammers, H. Lee SweeneyBiologicals), α-SMA (1:1000; #7817; Abcam), SMOC2 (1:500; #AF5140; R&D Systems), and PDGFRα (1:500; #AF1062; R&D Systems). Image acquisition was performed with a Leica Application Suite X software on a Leica

(9) Villification of the intestinal epithelium is driven by Foxl1 through activation of PDGFRα and BMPsNature CommunicationsFebruary 24, 2026Guoli Zhu, Galina Rozenberg, Deeksha Lahori, Jonathan Schug, Mark Tigue, Lan Cheng, Kirill Batmanov, Klaus H. Kaestnerin the dark for 1 h. The following antibodies were used: goat anti-PDGFR alpha 1: 200 (R&D Systems, AF1062), mouse anti-EZRIN 1:1000 (Sigma-Aldrich, E8897), rat anti-E-Cadherin antibody [DECMA-1] 1: 200 (Abcam,

(10) BMP2-induced Adam12 + Fibroblasts Dictate Wound-associated Skin Scarring and FibrosisInternational Journal of Biological SciencesFebruary 4, 2026Jun-Yi Chen, Jin-Ru Song, Ke-Ai Li, Xue-Yan Xu, Ding-Heng Zhu, Lian Zhang, Zi-Shuo Chen, Qing Cheng, Liu-Yi Yao, Yi-Qi Shen, et al.I (ab270993, Abcam) , anti-Collagen III (ab7778, Abcam), anti-BMP2 (ab214821, Abcam), anti-PDGFRα (AF1062, R&D Systems) and anti-p-Smad1/5/8 (13820, Cell Signaling Technology) were incubated overnight at 4

(11) Microglia replacement by peripheral delivery of CSF1R inhibitor-resistant hematopoietic cellsMolecular TherapyFebruary 4, 2026Sonia I. Lombroso, Emily I. Church, Ryan N. Anderson, Carleigh A. O’Brien, Yixuan Zhu, Asif A. Dar, Freddy S. Purnell, Eli M. Levitt, Brian Temsamrit, Fazeela Yaqoob, et al.goat anti-IBA1 (1:250; Abcam, catalog no. ab5076), goat anti-PDGFRα: (1:400; R&D Systems, catalog no. AF1062), and rabbit anti-double cortin (1:500, CST, catalog no. 4604S). After washing 3 × 5 min in 1× PBS,

(12) Midbrain PAG Astrocytes Modulate Mouse Defensive and Panic‐Like BehaviorsAdvanced ScienceFebruary 4, 2026Ellane Barcelon, Kyungchul Noh, Minkyu Hwang, Yoon‐Jung Kim, Unjin Lee, Yeon Joo Ryu, Je‐Kyung Ryu, Sang Beom Jun, Se‐Young Choi, Woo‐Hyun Cho, et al.Synaptic systems, Göttingen, Germany), VGAT (#131006, 1:1,000; Synaptic systems), mouse anti‐PDGFRa (AF1062, 1:1000; R&D systems, Minneapolis, MN, USA), and mouse anti‐CC1 (OP‐80, 1:1000; Millipore) and rabbit

(13) Hypertension-induced neurovascular and cognitive dysfunction at single-cell resolutionNeuronFebruary 4, 2026Samantha M. Schaeffer, Anthony G. Pacholko, Monica M. Santisteban, Sung Ji Ahn, Gianfranco Racchumi, Gang Wang, Laibaik Park, Giuseppe Faraco, Josef Anrather, Costantino IadecolaOPC were identified using anti-Olig2 (1:200, rabbit, ab109186, Abcam) and anti-Pdgfrα (1:200, goat, AF1062, Biotechne), and double-stranded DNA breaks were detected with anti-phospho(Ser139)-histone H2A.X (1:100,

(14) Efficient in vivo targeting of the myocardial scar using Moloney murine leukaemia virus complexed with nanoparticlesThe Journal of PhysiologyFebruary 1, 2026Timo Mohr, Miriam Schiffer, Deepak Ramanujam, Esther Carls, Callum M. Zgierski‐Johnston, Thomas Kok, Pia Niemann, Caroline Geisen, Peter Kohl, Stefan Engelhardt, et al.IgY,IHC: 1:500,NBP2‐25158,Novus Biologicals,Zhou et al. (2018) PDGFRα,Goat polyclonal IgG,IHC: 1:200,AF1062,"R&D Systems, Minneapolis, MN, USA",Waisman et al. (2021) P2A (3H4),Mouse monoclonal IgG1 kappa,IHC:

(15) “PDGFRα is required for postnatal cerebral perivascular fibroblast development”Developmental BiologyFebruary 1, 2026Hannah E. Jones, Kelsey A. Abrams, Sol Kim, Katherine A. Fantauzzo, Julie A. Siegenthalerused: mouse anti-Collagen1a1 (1:100, Sigma-Aldrich, SAB1402151), goat anti-PDGFRα (1:100, R&D Systems, AF1062), rat anti-PDGFRβ (1:100, Novus, NBP1–43349), rat anti-CD31 (PECAM) (1:100, BD Pharmigen, 550274), rabbit

(16) Dying oligodendrocytes persist without mitochondriabioRxivJanuary 25, 2026Xhoela Bame, S. Zoela Gilani, Yasmine Kamen, Robert A. Hill#aa82–87), rabbit anti-BCAS1 (1:750, Synaptic Systems, #445 003), goat anti-PDGFRA (1:1000, R&D Systems, #AF1062), rabbit anti-IBA1 (1:500, FUJIFILM Wako, #019–19741), rabbit anti-GFAP (1:500, Sigma-Aldrich, #G9269),

(17) Essential role of NONO-HOXA1-Wnt axis in cardiomyocyte differentiationNature CommunicationsJanuary 24, 2026Zhiyu Feng, Yuan Gao, Han Gao, Siyu Sun, Weilan Na, Xianghui Huang, Shuolin Li, Chaozhong Tan, Shaojie Min, Yuquan Lu, et al.lineage analyses, dissociated cells were separately stained with antibodies against PDGFRA (R&D Systems, AF1062), NKX2-5 (CST, 8792), or GATA4 (Santa Cruz, sc-25310) using the same fixation and permeabilization conditions.

(18) The transition from monocyte to tissue-resident macrophage requires DHPSNatureJanuary 21, 2026Gustavo E. Carrizo, Pianpian Lin, Seung Hyun Lee, Kevin Shenderov, Camille Blériot, Minsun Cha, Lena Schimmelpfennig, Zhen Shen, Nikki van Teijlingen Bakker, Katarzyna M. Grzes, et al.ImageJ was used for measurement. The following primary antibodies were used: anti-PDGFRα (R&D Systems, AF1062, polyclonal, 1:200 dilution), anti-F4/80 (Bio-Rad, MCA497GA or MCA497B, clone Cl:A3-1, 1:300 or 1:50),

(19) The Microbiota Shapes Central Nervous System Myelination in Early LifeAdvanced ScienceJanuary 18, 2026Caoimhe M. K. Lynch, Emily G. Knox, Daniel Soong, Thomaz F. S. Bastiaanssen, Kalevi Trontti, Gabriel S. S. Tofani, Sophia Ivaschuk, Michael K. Collins, Donia Arafa, Jatin Nagpal, et al.primary antibodies: rabbit anti‐Olig2 (1:250, Merck, ab9610), goat anti‐PDGFR⍺ (1:500, R&D Systems, af1062), and mouse anti‐CNPase (1:2000, Atlas Antibodies, AMAb91072) in an antibody buffer (1% NDS, 1% BSA,

(20) Temporal Dynamics of CNS Cholesterol Esters Correlate with Demyelination and RemyelinationACS Chemical NeuroscienceJanuary 7, 2026Nishama De Silva Mohotti, Jenna M. Williams, Rashmi Binjawadagi, Hiroko Kobayashi, Disni Dedunupitiya, Jack M. Petersen, Alana Garcia, Meredith D. Hartleyfor GFAP (EMD Millipore AB5541, 1:500), IBA1 (Fujifilm Wako 019–19741, 1:500), PDGFRα (R&D Systems AF1062, 1:500), Aspa/Nur7 (Millipore ABN1698, 1:100), GST-pi (MBL Life Science 312, 1:500), ACAT1 (Proteintech

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9/30/2022R&D's Polyclonal Anti-Mouse PDGF R Alpha Stains Immature Oligodendrocytes Fabrizio BiundoWe identify and characterize mature oligodendrocytes in mouse brain sections from murine models of neurodegeneration and aging using this polyclonal antibody reagent from R&D Systems.