Neogenin Antibody AF1079 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemNeogenin Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF1079
NCBI Gene AliasesNGN, NTN1R2, 2610028H22Rik, IGDCC2, Igdcc2, D930014N22Rik
ApplicationsBioassay,Co-Immunoprecipitation,FACS,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Simple Western,Blockade of Receptor-ligand Interaction,Neutralization,Immunocytochemistry
IsotypeIgG
ReactivityAvian - Chicken,Human,Mouse,Primate - Chlorocebus pygerythrus (Vervet Monkey),Transgenic Mouse
HostGoat
TargetNeogenin
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
NCBI Full Gene Nameneogenin, neogenin 1
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse Neogenin, Ala42-Ile1033 (Asp442-Leu461 del), Accession # NP_032710
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human and mouse Neogenin in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse DCC is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study Neogenin in mouse models?

Yes, the Neogenin antibody has been shown to work effectively in mouse models, as evidenced by its use in immunoblotting to detect Neogenin protein in various studies, including those examining olfactory epithelium differentiation and axon guidance (Kam et al., 2016; Liu et al., 2018).

Is this antibody suitable for immunofluorescence applications?

The Neogenin antibody has been utilized in immunofluorescence applications, as indicated in studies where it was employed to visualize Neogenin expression in cultured neurons and other tissues (Sempert et al., 2023; Shohayeb et al., 2024).

What specific roles has Neogenin been implicated in according to the literature?

Neogenin has been implicated in various biological processes, including neuronal differentiation, apoptosis, and migration, particularly in the context of neural development and cancer metastasis (Van den Heuvel et al., 2013; Untiveros et al., 2022).

How does the Neogenin antibody perform in Western blotting?

The Neogenin antibody has been successfully used in Western blotting to detect Neogenin protein levels in different experimental setups, including studies on dendritic spine morphogenesis and the effects of Netrin-1 on melanoma cell migration (Sempert et al., 2023; Untiveros et al., 2022).

R&D Systems, a Bio-Techne Brand

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Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Netrin-1 Promotes Pancreatic Tumorigenesis and Innervation through NEO1Cancer ResApril 15, 2026Hiroki Kobayashi, Yosuke Ochiai, Junya Arai, Masahiro Hata, Feijing Wu, Masaki Sunagawa, Tadashi Iida, Taisuke Baba, Ermanno Malagola, Takayuki Tanaka, et al.recombinant Netrin-1 (rNTN1; 100 ng/mL; 1109-N1; R&D Systems) and NEO1-blocking antibody (NEO1 Ab; 500 ng/mL; AF1079; R&D Systems, RRID: AB_11019023). After 7 days of treatment with rNTN1 and NEO1 Ab, images were captured

(2) The uncoordinated‐5 homologue A is a key receptor in netrin‐ligand‐mediated fast‐twitch myotube formation in male micePhysiological ReportsFebruary 17, 2026Takahiro Maeno, Tomoki Ushijima, Koichi Ojima, Yohei Ogawa, Sayuki Hayashi, Hikaru Imakyure, Rika Osaki, Ryuki Oyama, Aoi Ogawa, Akimasa Takano, et al.*for ICC UNC5A,Rabbit,"20,239‐1‐AP",Proteintech,1:500 UNC5B,Goat,AF1006,R&D Systems,1:500 UNC5C,Goat,AF1005,R&D Systems,1:1000 Neogenin,Goat,AF1079,R&D Systems,1:500 Total MyHC,Mouse,MAB4470,R&D Systems,1:500 *1:50 Slow MyHC,Mouse,M8421,Sigma‐Aldrich,1:500

(3) Uncovering the molecular logic of cortical wiring between neuronal subtypes across development through ligand–receptor inferenceNature CommunicationsJanuary 22, 2026Rémi Mathieu, Tangra Draia-Nicolau, Léa Corbières, Annousha Govindan, Vianney Bensa, Emilie Pallesi-Pocachard, Lucas Silvagnoli, Alfonso Represa, Carlos Cardoso, Ludovic Telley, et al.PA5-36472) and anti-Mouse GLUD1 (Proteintech, 67026-1-Ig) or anti-CBLN4 and goat anti-NEO1 (Biotechne, AF1079) co-immunolabeling before application of Rabbit Plus and Mouse Minus or Rabbit Plus and Goat Minus probes,

(4) CCR5 marks a subset of mouse hematopoietic stem cells that are myeloid primed and expand with ageProceedings of the National Academy of SciencesJanuary 20, 2026Leyla Yılmaz, Allison Banuelos, Michelle Baez, Uyen Le, Nardin Georgeos, Monika Zukowska, Allison Zhang, Rahul Sinha, Irving L. Weissman(PE-Cy7 or BV421). Neogenin1 was detected using a polyclonal primary antibody (R&D Systems, Catalog #: AF1079). Samples were washed with FACS buffer and cells were stained with a donkey anti-goat Alexa Fluor 488

(5) Netrin-1 Promotes Pancreatic Tumorigenesis and Innervation through NEO1Cancer ResearchDecember 31, 2025Hiroki Kobayashi, Yosuke Ochiai, Junya Arai, Masahiro Hata, Feijing Wu, Masaki Sunagawa, Tadashi Iida, Taisuke Baba, Ermanno Malagola, Takayuki Tanaka, et al.recombinant Netrin-1 (rNTN1; 100 ng/mL; 1109-N1; R&D Systems) and NEO1-blocking antibody (NEO1 Ab; 500 ng/mL; AF1079; R&D Systems, RRID: AB_11019023). After 7 days of treatment with rNTN1 and NEO1 Ab, images were captured

(6) Netrin-1 promotes pancreatic tumorigenesis and innervation through NEO1bioRxiv : the preprint server for biologyJuly 26, 2025Ochiai Y, Kobayashi H, Sunagawa M, Baba T, Malagola E, Wu F, Tanaka T, Hata M, Arai J, Jiang Z, et al.

(7) Netrin‐4 Modulates Corneal Wound Healing by Enhancing Corneal Epithelium and Nerve RegenerationFASEB BioAdvancesMay 14, 2025Cissy Xiao, Daniel Lara‐Newman, Qiang Zhou, Mark I. Rosenblatt, Victor H. Guaiquilconcentration of Netrin‐4 used in our assays: 0.2 μg/mL anti‐DCC (AF844), 1.5 μg/mL anti‐Neogenin (AF1079), 0.5 μg/mL anti‐UNC5H1 (AF1405), or 0.5 μg/mL anti‐Integrin α6β1 (MAB78009) antibodies, all from R&D systems

(8) BDNF-dependent nano-organization of Neogenin and the WAVE regulatory complex promotes actin remodeling in dendritic spinesiScienceSeptember 20, 2024Belal Shohayeb, Kai Sempert, Tristan P. Wallis, Frédéric A. Meunier, Nela Durisic, Elizabeth A. O'Brien, Cecilia Flores, Helen M. CooperIF",Sigma,Cat#M4439; RRID: AB_439694 "Neogenin goat polyclonal antibody, 1:250 WB, IF",R&D Systems,Cat#AF1079; RRID: AB_2151002 "Neogenin (C-20) goat polyclonal antibody, 1:250 IF",Santa Cruz,Cat#sc-6536; RRID:

(9) RGMa and Neogenin control dendritic spine morphogenesis via WAVE Regulatory Complex-mediated actin remodelingFrontiers in Molecular NeuroscienceOctober 19, 2023Kai Sempert, Belal Shohayeb, Vanessa Lanoue, Elizabeth A. O’Brien, Cecilia Flores, Helen M. Coopertemperature (RT) for 1 h and then with secondary antibodies (RT, 1 h). Primary antibodies: Neogenin, AF1079, R&D Systems, 1:200; GAPDH, CSB-PA00025A0rb, Cusa Biotechnology, 1:1000; ab3280, Abcam, 1:1000. Secondary

(10) Expression of axon guidance ligands and their receptors in the cornea and trigeminal ganglia and their recovery after corneal epithelium injuryExperimental eye researchJune 1, 2022Guaiquil VH, Xiao C, Lara D, Dimailig G, Zhou Q.tg",Donkey anti goat Alexa green 448,1:400,ThermoFisher A11055 Neogenin,ThermoFisher A11055,,,R&D Systems AF1079,, UNC5H1,Goat,1:200,mc,R&D Systems AF1405,Donkey anti goat Alexa green 488,1:400,ThermoFisher A11055 UNC5H1,R&D

(11) Simultaneous binding of Guidance Cues NET1 and RGM blocks extracellular NEO1 signalingCellApril 15, 2021Robinson RA, Griffiths SC, van de Haar LL, Malinauskas T, van Battum EY, Zelina P, Schwab RA, Karia D, Malinauskaite L, Brignani S, et al.Thermo-Fisher). Immunocytochemistry for DCC and NEO1 were performed with goat anti-Neogenin (1:200, AF1079; R&D systems) and goat anti-DCC (1:500, sc-6535, Santa Cruz) primary antibodies and Alexa Fluor 488

(12) Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizerNature communicationsOctober 14, 2020Apóstolo N, Smukowski SN, Vanderlinden J, Condomitti G, Rybakin V, Ten Bos J, Trobiani L, Portegies S, Vennekens KM, Gounko NV, et al.anti-ROBO2,Aviva Systems,Cat#: ARP45396_P050;RRID: AB_2047840 Goat anti-Neogenin,R&D Systems,Cat#: AF1079;RRID: AB_2151002 Sheep anti-SALM2,R&D Systems,Cat#: AF5669;RRID: AB_1964706 Mouse anti-Tenascin-R,R&D Systems

(13) Macrophage-derived netrin-1 promotes abdominal aortic aneurysm formation by activating MMP3 in vascular smooth muscle cellsNature communicationsNovember 27, 2018Hadi T, Boytard L, Silvestro M, Alebrahim D, Jacob S, Feinstein J, Barone K, Spiro W, Hutchison S, Simon R, et al.mouse anti-Human CD68 (MCA5709, Bio-Rad), rabbit anti-mouse/human MMP3 (ab53015, Abcam), goat anti -mouse neogenin-1 (AF1079, R&D Systems) or anti-mouse alpha-smooth muscle actin (ab7817, Abcam) (1:500 dilution each). Secondary

(14) Inhibition of neogenin fosters resolution of inflammation and tissue regenerationThe Journal of clinical investigationOctober 1, 2018Schlegel M, Körner A, Kaussen T, Knausberg U, Gerber C, Hansmann G, Jónasdóttir HS, Giera M, Mirakaj V.hours. GM-CSF–differentiated MΦ (0.1 × 106 cells/well) were then incubated with human Neo1 Ab (R&D Systems, catalog AF1079) or IgG control (Santa Cruz Biotechnology, catalog sc-2028) for 15 minutes. Apoptotic PMNs were added

(15) Tbr1 instructs laminar patterning of retinal ganglion cell dendritesNature neuroscienceMay 1, 2018Liu J, Reggiani JDS, Laboulaye MA, Pandey S, Chen B, Rubenstein JLR, Krishnaswamy A, Sanes JR.(1:500, Abcam ab31), goat anti-Alcam (1:1000, R&D systems AF117258), goat anti-Neo1 (1:1000, R&D systems AF1079, Supplementary Figure 8c) and rabbit β-galactosidase (1:5000 Duan et al. 201410). Dylight405-, Alexa488-,

(16) RGMB and neogenin control cell differentiation in the developing olfactory epitheliumDevelopment (Cambridge, England)May 1, 2016Kam JW, Dumontier E, Baim C, Brignall AC, Mendes da Silva D, Cowan M, Kennedy TE, Cloutier JF.the function-blocking antibody experiments, 5 μg/ml control IgG or neogenin antibodies (R&D Systems, AF1079) were added to the media. Explants were grown on merosin following previously described protocols (DeHamer et

(17) Effect of Erythropoietin, Iron Deficiency and Iron Overload on Liver Matriptase-2 (TMPRSS6) Protein Content in Mice and RatsPloS oneJanuary 1, 2016Frýdlová J, Přikryl P, Truksa J, Falke LL, Du X, Gurieva I, Vokurka M, Krijt J.anti-TMPRSS6, Ab56182, Abcam, 1:750; goat anti-HFE2, AF 3634, R&D Systems, 1:1500; goat anti-neogenin, AF1079, R&D Systems, 1:1000, and rabbit anti- Na+/K+ATPase α, SC-28800 (ATP1A), Santa Cruz, 1:10 000. Secondary

(18) Spatiotemporal expression of repulsive guidance molecules (RGMs) and their receptor neogenin in the mouse brainPloS oneJanuary 1, 2013van den Heuvel DM, Hellemons AJ, Pasterkamp RJ.R&D systems) 1∶200, sheep anti-RGMb antibody (AF3597; R&D systems) 1∶50 or goat anti-Neogenin antibody (AF1079; R&D systems) 1∶50 in normal blocking buffer for 2 hrs at RT. Cells were washed in PBS and incubated

(19) Genetic analysis of DSCAM's role as a Netrin-1 receptor in vertebratesThe Journal of neuroscience : the official journal of the Society for NeuroscienceJanuary 11, 2012Palmesino E, Haddick PC, Tessier-Lavigne M, Kania A.

(20) The axonal guidance receptor neogenin promotes acute inflammationPloS oneJanuary 1, 2012König K, Gatidou D, Granja T, Meier J, Rosenberger P, Mirakaj V.

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