Mouse TSLP Antibody AF555 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse TSLP Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF555
ApplicationsImmunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Neutralization,Immunocytochemistry/ Immunofluorescence
Quantity100 ug (also 25 ug)
TargetTSLP
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
HostGoat
IsotypeIgG
ReactivityHuman,Mouse,Transgenic Mouse
NCBI Full Gene Namethymic stromal lymphopoietin
ClonalityPolyclonal
ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TSLP, Tyr20-Glu140, Accession # Q9JIE6
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this TSLP antibody be used for immunofluorescence applications?

Yes, the TSLP antibody has been shown to work in immunofluorescence applications, as indicated by its use in studies involving the visualization of specific proteins in cells.

What species does this antibody react with?

The TSLP antibody is reactive with both mouse and human species, making it suitable for experiments involving these organisms.

Has this antibody been utilized in any specific research studies?

Yes, the TSLP antibody has been cited in at least 31 scientific articles, indicating its application in various research contexts, including studies related to immune responses and signaling pathways.

What is the recommended storage condition for this antibody after reconstitution?

After reconstitution, the TSLP antibody should be stored under sterile conditions at -20 to -70 °C for up to six months to maintain its stability and effectiveness.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Pre-assembly of biomolecular condensate seeds drives RSV replicationNatureApril 2, 2026Dhanushika Ratnayake, Marie Galloux, Sanne Boersma, Marko Noerenberg, Christina Sizun, Carlos Sacristan, Julien Sourimant, Anke J. Lakerveld, Anne T. Gelderloos, Leonie Apperloo, et al.labelled using N-hydroxysuccinimide (NHS)-esters (Alexa Fluor 555 NHS ester and Alexa Fluor 647 NHS ester; AF555, A20009, AF647 and A20106, Thermo Fisher Scientific) for use in immunofluorescence. The antibody was

(2) Avenanthramide C and chondroitin sulphate promote chondrogenic differentiation of adipose-derived mesenchymal stem cellsScientific ReportsMarch 11, 2026Priscila Sun, Winston Lim, Shivatra C. Talchai, Veerapol Khemarangsanantibody, Rabbit anti-LDL Receptor Related Protein 1 (LRP1; ab92544) primary antibody and Goat anti-rabbit AF555 (ab150078) was purchased from Abcam, US. Bovine Serum Albumin (BSA; 0219989880) was procured from MP Biomedicals

(3) Explainable AI-based analysis of human pancreas sections identifies traits of type 2 diabetesNat CommunFebruary 9, 2026Lukas Klein, Sebastian Ziegler, Felicia Gerst, Yanni Morgenroth, Karol Gotkowski, Eyke Schöniger, Martin Heni, Nicole Kipke, Daniela Friedland, Annika Seiler, et al.and tubulin beta 3 (1:25; R&D Systems, MAB1195), for the second incubation against mouse IgG1 con. AF555 (1:200; Invitrogen, #A-21127) and mouse IgG2a con. AF647 (1:50; Invitrogen, #A21241), and for the third

(4) Positioning and reversible suppression of CCR7+ dendritic cells in perivascular tumor niches shape cancer immunityImmunityJanuary 13, 2026Beatrice Zitti, Florent Duval, Pratyaksha Wirapati, Mehdi Hicham, Yuxuan Xie, Juhyun Oh, Jan Hoelzl, Philippa Meiser, Marco Varrone, Hannah M. Peterson, et al.AB_2572995 Unconjugated rat anti-human MECA-79 (Clone MECA-79),Novus Biologicals,Cat# NB100-77673SS AF555 rabbit anti-human CD45 (Clone AKYP0074),Akoya Biosciences,Cat#240060 AF750 mouse anti-human panCK (Clone

(5) FNA biopsy of breast specimens effectively harvests cells for patient-derived organoids modeling ductal carcinoma in situCancer cytopathologyNovember 1, 2025Ye J, Goldhammer N, Vohra P, Warhadpande S, de Castro GC, Rodas CKM, Moasser MM, Ramalingam K, Abe SE, Alvarado M, et al.AF488, clone LL002 (Novus Biologicals, NBP2‐34675AF488) at 1:100 dilution, and Keratin 19 conjugated to AF555, clone EP1580Y (Abcam, ab203444) at 1:100 dilution. After washing, organoids were incubated with 4′,6‐diamidino‐2‐phenylindole,

(6) Increased mitochondrial fusion via systemic OPA1 overexpression promotes dyslipidemia and atherosclerosis in LDLR deficient miceMolecular MetabolismSeptember 22, 2025Lorenzo Da Dalt, Francesca Fantini, Giulia Giancane, Annalisa Moregola, Silvia Roda, Monika Svecla, Silvia Pedretti, Giovanni Battista Vingiani, Jiangming Sun, Andreas Edsfeldt, et al.(1:1000; Cat#A21434, Invitrogen) or anti-αSMA (1:200; Cat#NBP2-67440, Bio-Techne) followed by anti-rabbit AF555 antibody (1:1000; Cat# A27039, Invitrogen) and nuclei counterstaining with Hoechst (Sigma) respectively

(7) Inflammatory cytokine interleukin-18 prevents murine miscarriage by inducing appropriate inflammationScientific reportsAugust 21, 2025Horii Y, Ino H, Sasaki F, Ishibashi M, Koike E, Kawamoto Y, Flavell RA, Ohashi R, Suzuki S, Negishi Y, et al.anti-mouse αSMA antibody (NBP2-33006; Novus Biologicals, Centennial, CO.) overnight at 4℃, followed by AF555-conjugated anti-rabbit IgG (A48283; Invitrogen) and AF488-conjugated anti-mouse IgG (A48286; Invitrogen)

(8) Development of an acute lung injury model for drug testingSci RepMay 21, 2025Jocelyn R. Grunwell, Susan T. Stephenson, Gail A. Dallalio, Badiallo A. Diani, Celena Zaworski, Natalie Jordan, Anne M. FitzpatrickEpithelial cell types were stained with MUC5AC-AF405 (1:200; Novus Biologicals, Centennial, Colo), SCGB3A2-AF555 (1:100; Abcam, Fremont, Calif), cytokeratin 5-AF488 (1:200; Abcam), and acetylated-tubulin-AF647 (1:100;

(9) Adipose-derived small extracellular vesicle miR-146a-5p targets Fbx32 to regulate mitochondrial autophagy and delay aging in skeletal muscleJournal of nanobiotechnologyApril 10, 2025Qin M, Wang Y, Wang Z, Dong B, Yang P, Liu Y, Xi Q, Ma J.at room temperature for 1 h with goat anti-mouse IgM conjugated to Alexa Fluor 555 (1:2000, bs-0368G-AF555, Bioss). Nuclei were DAPI-stained. Fluorescent images were acquired with a Nikon Eclipse Ti microscope,

(10) Sterile production of interferons in the thymus affects T cell repertoire selectionScience ImmunologyJuly 26, 2024K. Maude Ashby, Matouš Vobořil, Oscar C. Salgado, S. Thera Lee, Ryan J. Martinez, Christine H. O’Connor, Elise R. Breed, Shuya Xuan, Charles R. Roll, Saumith Bachigari, et al.overnight at 4°C followed by three washes with PBS. Secondary staining was performed with streptavidin-AF555 (Life Technologies) or AF555 donkey anti-rabbit IgG H+L (Life Technologies) for 30 minutes at room temperature.

(11) Multi-omic profiling of follicular lymphoma reveals changes in tissue architecture and enhanced stromal remodeling in high-risk patientsCancer cellMarch 11, 2024Radtke AJ, Postovalova E, Varlamova A, Bagaev A, Sorokina M, Kudryashova O, Meerson M, Polyakova M, Galkin I, Svekolkin V, et al.RRID: AB_2534076 PD-1 (polyclonal),Novus Biologicals,Cat#AF1086; RRID: AB_354588 Donkey anti-goat IgG AF555,Thermo Fisher Scientific,Cat#A-21432; RRID: AB_2535853 Hoechst,Biotium,Cat#40046; RRID: N/A IRF4 (clone

(12) Dysfunctional neutrophil Type 1 interferon responses in preschool children with recurrent wheezing and IL-4 mediated aeroallergen sensitizationJournal of Allergy and Clinical Immunology: GlobalFebruary 13, 2024Anne M. Fitzpatrick, Min Huang, Ahmad F. Mohammad, Susan T. Stephenson, Rishikesan Kamaleswaran, Jocelyn R. GrunwellUnited Kingdom) after staining for MUC5AC-AF405 (1:200; Novus Biologicals, Centennial, Colo), SCGB3A5-AF555 (1:100; Abcam, Fremont, Calif), cytokeratin 5-AF488 (1:200; Abcam), and acetylated-tubulin-AF647 (1:100;

(13) The dePARylase NUDT16 promotes radiation resistance of cancer cells by blocking SETD3 for degradation via reversing its ADP-ribosylationJournal of Biological ChemistryJanuary 23, 2024Weijun Wu, Wenjing Wu, Yingshi Zhou, Qiao Yang, Shuting Zhuang, Caixia Zhong, Wenjia Li, Aixin Li, Wanzhen Zhao, Xiaomin Yin, et al.(4336-BPC-100) antibody was obtained from R&D Systems. Human anti-PARP1 (556494), anti-53BP1 (612522), and anti-AF555-γH2AX (560446) antibodies were purchased from BD Biosciences. Anti-Flag (66008-4-Ig) and anti-TNKS1

(14) p-tau Ser356 is associated with Alzheimer's disease pathology and is lowered in brain slice cultures using the NUAK inhibitor WZ4003Acta neuropathologicaJanuary 4, 2024Taylor LW, Simzer EM, Pimblett C, Lacey-Solymar OTT, McGeachan RI, Meftah S, Rose JL, Spires-Jones MP, Holt K, Catterson JH, et al.antibodies for 1 h: 1:500 Rabbit p-tau Ser356 (Abcam: ab75603), 1:100 goat synaptophysin (R&D Systems: AF555), 1:200 chicken GFAP (Abcam: ab4674), 1:200 guinea pig PSD95 (Synaptic Systems: 124 014) and 1:100 mouse

(15) Thyroid cancers exhibit oncogene-enhanced macropinocytosis that is restrained by IGF1R and promotes albumin-drug-conjugate responseClinical Cancer ResearchSeptember 1, 2023Huiyu Hu, Thomas S.C. Ng, Mikyung Kang, Ella Scott, Ran Li, Jeremy M. Quintana, Dylan Matvey, Venkata R. Vantaku, Ralph Weissleder, Sareh Parangi, et al.anti-IGF1R mAb (R&D Systems), and/or vehicle. 5 μM of Macrin-VT680 (to mark macrophages)(32) and 1 μM HSA-AF555 were incubated for another 4 h before 4% PFA fixation. Microscopy and flow cytometry. Confocal microscopy

(16) Spatiotemporally organized immunomodulatory response to SARS-CoV-2 virus in primary human broncho-alveolar epitheliaiScienceAugust 18, 2023Castaneda DC, Jangra S, Yurieva M, Martinek J, Callender M, Coxe M, Choi A, García-Bernalt Diego J, Lin J, Wu TC, et al.Sinai,NP-1C7 Anti-cytokeratin 5 AF594 (Polyclonal),Novus,Cat#NBP2-61931AF594RRID: AB_2933967 Anti-mouse IgG2b AF555,Thermo Fisher,Cat#A-21147RRID: AB_2535783 Anti-rabbit IgG AF555,Biolegend,Cat#406412RRID: AB_2563181 Anti-rat

(17) Unlocking the Role of Endothelial MPL Receptor and JAK2V617F Mutation: Insights into Cardiovascular Dysfunction in MPNs and CHIPbioRxiv : the preprint server for biologyJuly 13, 2023Zhang H, Kafeiti N, Lee S, Masarik K, Zheng H, Zhan H.1:250). After three washes with PBS, the cells were incubated with donkey anti-goat IgG conjugated to AF555 (Cat. A21432, ThermoFisher; 1:500 dilution) for 30minutes to 1 hour at room temperature. Following another

(18) Signalling by senescent melanocytes hyperactivates hair growthNatureJune 1, 2023Wang X, Ramos R, Phan AQ, Yamaga K, Flesher JL, Jiang S, Oh JW, Jin S, Jahid S, Kuan CH, et al.anti-Pcad (1:200; AF761, R&D Systems). The following secondary antibodies were used: donkey anti-rat AF555 (1:1,000; ab150154, Abcam), donkey anti-rabbit AF555 (1:1,000; A31572, Thermo Fisher), donkey anti-mouse

(19) Non-Expressed Donor KIR3DL1 Alleles May Represent a Risk Factor for Relapse after T-Replete Haploidentical Hematopoietic Stem Cell TransplantationCancersMay 13, 2023Legrand N, Salameh P, Jullien M, Chevallier P, Ferron E, David G, Devilder MC, Willem C, Gendzekhadze K, Parham P, et al.(clone 177407; R&D Systems), the corresponding IgG1 isotype control (clone 11711; R&D Systems), the AF555-conjugated anti-HLA class I mAb (clone F41-1E3), and the corresponding AF555-IgG1 isotype control (clone

(20) Transcriptomic atlas and interaction networks of brain cells in mouse CNS demyelination and remyelinationCell reportsApril 25, 2023Hou J, Zhou Y, Cai Z, Terekhova M, Swain A, Andhey PS, Guimaraes RM, Ulezko Antonova A, Qiu T, Sviben S, et al.AB_312981 anti-Carbonic Anhydrase II/CA2 (rat monoclonal),R&D Systems,Cat#MAB2184; RRID: AB_2065858 anti-rabbit IgG AF555 (donkey polyclonal),Abcam,Cat#150074; RRID: AB_2636997 anti-goat IgG AF647 (donkey polyclonal),Abcam,Cat#150131;

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TSLP Antibody

Mouse TSLP Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (53)

Reviews

Mouse TSLP Antibody from R&D Systems, a Bio-Techne Brand

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