Mouse EphB3 Antibody AF432 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse EphB3 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $479.00Supplier Page View Company Product Page
Catalog NumberAF432
NCBI Gene AliasesCek10, HEK2, Tyro6, mDK-5, Sek4, Etk2, MDK5, ETK2, TYRO6, EK2
ApplicationsFlow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunofluorescence,Immunocytochemistry,CyTOF-ready
ReactivityHuman,Mouse,Rat
IsotypeIgG
Conjugate/TagUnconjugated
HostGoat
Quantity100 ug
TargetEphB3
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
NCBI Full Gene NameEph receptor B3, EPH receptor B3
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse EphB3, Leu30-Thr537, Accession # P54754
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse EphB3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human EphB3 is observed and less than 1% cross-reactivity with recombinant rat EphB1, recombinant mouse (rm) EphA4, rmEphB2, rmEphB4, and rmEphB6 is observed.

Sizes Available: 100 ug

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Retrograde movements determine effective stem cell numbers in the intestineNatureJuly 1, 2022Azkanaz M, Corominas-Murtra B, Ellenbroek SIJ, Bruens L, Webb AT, Laskaris D, Oost KC, Lafirenze SJA, Annusver K, Messal HA, et al.stained with Ephrin B2-antibody (1:100 R&D system, AF467) and Ephrin B3-antibody (1:100 R&D system, AF432). Stainings were finalized by incubation with Alexa Fluor secondary antibodies, donkey anti-goat IgG

(2) Tracheal separation is driven by NKX2-1-mediated repression of Efnb2 and regulation of endodermal cell sortingCell reportsMarch 15, 2022Lewis AE, Kuwahara A, Franzosi J, Bush JO.

(3) CD74 Signaling Links Inflammation to Intestinal Epithelial Cell Regeneration and Promotes Mucosal HealingCellular and molecular gastroenterology and hepatologyJanuary 1, 2020Farr L, Ghosh S, Jiang N, Watanabe K, Parlak M, Bucala R, Moonah S.

(4) Unidirectional Eph/ephrin signaling creates a cortical actomyosin differential to drive cell segregationThe Journal of cell biologyOctober 24, 2016O'Neill AK, Kindberg AA, Niethamer TK, Larson AR, Ho HH, Greenberg ME, Bush JO.immunofluorescence using 2 µg/ml ephrin-B1 (AF473; R&D Systems), 2 µg/ml EphB2 (AF467; R&D Systems), 2 µg/ml EphB3 (AF432; R&D Systems), and 10 µg/ml GFP (ab13970; Abcam) antibodies. The following secondary antibodies were

(5) Why some tumours trigger neovascularisation and others don't: the story thus farChinese journal of cancerFebruary 12, 2016Adighibe O, Leek RD, Fernandez-Mercado M, Hu J, Snell C, Gatter KC, Harris AL, Pezzella F.Keynes, UK)" CXCR4,MAB 172,"R&D System (Minneapolis, MN, USA)" EPHB2,AF496,R&D System EPHB3,R&D AF432,R&D System EPHB4,R&D AF446,R&D System SOD1,30F11,Novocastra BCL2,124,"Dako (Cambridgeshire, UK)" FOS,Polyclonal

(6) Tissue-Specific Effects of Reduced β-catenin Expression on Adenomatous Polyposis Coli Mutation-Instigated Tumorigenesis in Mouse Colon and Ovarian EpitheliumPLoS geneticsNovember 1, 2015Feng Y, Sakamoto N, Wu R, Liu JY, Wiese A, Green ME, Green M, Akyol A, Roy BC, Zhai Y, et al.

(7) Aberrant EphB/ephrin-B expression in experimental gastric lesions and tumor cellsWorld journal of gastroenterologyJanuary 14, 2015Uchiyama S, Saeki N, Ogawa K.RT-PCR experiments. Antibodies Goat polyclonal antibodies against the extracellular domains of mouse EphB2 (AF467), EphB3 (AF432), EphB4 (AF446), and ephrin-B1 (AF473) were obtained from R&D Systems, Inc. (Minneapolis, MN, United States).

(8) Distinct fibroblast lineages determine dermal architecture in skin development and repairNatureDecember 12, 2013Driskell RR, Lichtenberger BM, Hoste E, Kretzschmar K, Simons BD, Charalambous M, Ferron SR, Herault Y, Pavlovic G, Ferguson-Smith AC, et al.PDGFRa (R&D Systems AF1062), Fabp4 (R&D Systems AF1443), AdipoQ (R&D Systems AF1119), Ephb3 (R&D Systems AF432), Lrig1 (R&D Systems AF3688), Podplanin (R&D Systems AF3244), Transglutaminase2 (R&D Systems AF4376),

(9) Smad3 contributes to positioning of proliferating cells in colonic crypts by inducing EphB receptor protein expressionBiochemical and biophysical research communicationsFebruary 25, 2011Furukawa K, Sato T, Katsuno T, Nakagawa T, Noguchi Y, Tokumasa A, Yokote K, Yokosuka O, Saito Y.

(10) A stem cell marker-expressing subset of enteroendocrine cells resides at the crypt base in the small intestineAmerican journal of physiology. Gastrointestinal and liver physiologyFebruary 1, 2011Sei Y, Lu X, Liou A, Zhao X, Wank SA.located at different cell 68 positions in the crypts and villi. Goat polyclonal anti-EphB3 antibody (AF432) was from 69 R&D Systems, Inc (Minneapolis, MN) and used at a concentration of 10 μg/ml. Alexa 70 Fluor

(11) Ephrin-B1 regulates axon guidance by reverse signaling through a PDZ-dependent mechanismGenes & developmentJuly 1, 2009Bush JO, Soriano P.

(12) The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganizationThe Journal of biological chemistryOctober 27, 2006Yang NY, Pasquale EB, Owen LB, Ethell IM.

(13) EphB receptors coordinate migration and proliferation in the intestinal stem cell nicheCellJune 16, 2006Holmberg J, Genander M, Halford MM, Annerén C, Sondell M, Chumley MJ, Silvany RE, Henkemeyer M, Frisén J.

(14) EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPasesJournal of cell scienceFebruary 1, 2006Ogawa K, Wada H, Okada N, Harada I, Nakajima T, Pasquale EB, Tsuyama S.

(15) Mule Regulates the Intestinal Stem Cell Niche via the Wnt Pathway and Targets EphB3 for Proteasomal and Lysosomal DegradationCell stem cellJanuary 1, 0001"Dominguez-Brauer C, Hao Z, Elia AJ, Fortin JM, Nechanitzky R, Brauer PM, Sheng Y, Mana MD, Chio IIC, Haight J, et al."(#A0099), ephrinB1 (R&D Systems, clone AF473), EphB2 (R&D Systems, clone AF467), EphB3 (R&D Systems, clone AF432), cleaved caspase-3 (Cell Signaling Technology, #9661), Ki67 (Dako, Tec3 clone), chromogranin A (Immunostar,

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Mouse EphB3 Antibody

EphB3 Antibody

EphB3 Antibody

EphB3 Antibody

Mouse EphB3 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (15)

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Mouse EphB3 Antibody from R&D Systems, a Bio-Techne Brand

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