PDGF-BB Antibody AF-220-NA from R&D Systems, a Bio-Techne Brand

Product Specs
ItemPDGF-BB Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF-220-NA
NCBI Gene AliasesSIS, IBGC5, c-sis, SSV, PDGF-2, PDGF2
ApplicationsImmunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Dot Blot,In vivo assay,Neutralization,Immunocytochemistry,ELISA Detection
Conjugate/TagUnconjugated
TargetPDGF-BB
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman,Mouse,Rabbit,Rat,Transgenic Mouse
IsotypeIgG
Quantity100 ug (also 25 ug)
HostGoat
NCBI Full Gene Nameplatelet derived growth factor subunit B
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human PDGF-BB, Ser82-Thr190, Accession # P01127
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects PDGF-BB in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used for immunohistochemistry applications?

Yes, the PDGF-BB antibody has been shown to work in immunohistochemistry applications, as evidenced by its use in studies where it was employed to assess PDGF-BB levels in tissue sections.

What species can this antibody detect?

The PDGF-BB antibody is reactive with several species, including human, mouse, rat, and rabbit, making it versatile for various experimental models.

Has this antibody been utilized in any specific experimental contexts?

Yes, the PDGF-BB antibody has been used in studies investigating neointimal hyperplasia and pulmonary arterial hypertension, where it was employed to measure PDGF-BB levels in tissue and cell culture supernatants, respectively.

What is the recommended dilution for using this antibody in blocking experiments?

In blocking experiments, the PDGF-BB antibody is typically used at a concentration of 5 µg/mL, as demonstrated in studies assessing its effects on cytokine and growth factor secretion.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Sulfated polysaccharide prevents senescent adipocyte-driven osteonecrosis by stem cell fate reprogrammingBioactive MaterialsJune 1, 2026Shuang Zhang, Shunshu Deng, Kai Dai, Yang Liu, Jing Wang, Changsheng Liuμg per dose, twice per week, administered for 4 weeks), PDGF-BB neutralizing antibody (R&D Systems, AF-220-NA; intraosseous injection, 2 μg per dose, twice per week, administered for 4 weeks), TGF-β1 neutralizing

(2) In Vitro Enhanced Performance of Human Platelet Lysate Gel Integrated with Mesoporous Silica Nanoparticle/Carboxymethyl Chitosan Composite Hydrogel: Structural Stability and Biological Activities for Chronic Wound HealingACS OmegaMarch 24, 2026Tareerat Lertwimol, Suwitchaya Jankam, Setthawut Kitpakornsanti, Weerachai Singhatanadgit, Wanida Janvikulactivity was inhibited by preincubating xPMC/hPLG spots with 0.2 μg/mL of a PDGF-BB neutralizing antibody (AF-220-NA, R&D Systems) for 45 min. As a negative control, xPMC/hPLG spots were pretreated for 45 min with an

(3) Platelet Responses to Urethane Dimethacrylate-Based Bone Cements Containing Monocalcium Phosphate/ε-Polylysine: Role of ε-Polylysine in In Vitro Wound Healing Induced by Platelet-Derived Growth Factor-BBACS Materials AuMarch 12, 2025Phatchanat Klaihmon, Piyarat Sungkhaphan, Boonlom Thavornyutikarn, Setthawut Kitpakornsanti, Praphasri Septham, Anne Young, Chanchao Lorthongpanich, Wanida Janvikul, Weerachai SinghatanadgitPRP, a PDGF-BB neutralization assay was employed. The neutralizing antibody against human PDGF-BB (#AF-220-NA, R&D systems) or an isotype control antibody (final concentration at 500 ng/mL) was added to pretreat

(4) Type H vessel/platelet-derived growth factor receptor β(+) perivascular cell disintegration is involved in vascular injury and bone loss in radiation-induced bone damageCell proliferationJuly 1, 2023Li J, Chen X, Ren L, Chen X, Wu T, Wang Y, Ren X, Cheng B, Xia J.

(5) A dynamic single cell-based framework for digital twins to prioritize disease genes and drug targetsGenome medicineMay 6, 2022Li X, Lee EJ, Lilja S, Loscalzo J, Schäfer S, Smelik M, Strobl MR, Sysoev O, Wang H, Zhang H, et al.presence of either a neutralizing anti-human IL-4 antibody (MAB204) or anti-human PDGF-BB antibody (AF-220-NA, both from R&D Systems, Minneapolis, USA) at a concentration of 5 μg/mL. Cell culture supernatants were

(6) Heme stimulates platelet mitochondrial oxidant production to induce targeted granule secretionRedox biologyDecember 1, 2021Annarapu GK, Nolfi-Donegan D, Reynolds M, Wang Y, Kohut L, Zuckerbraun B, Shiva S.

(7) DutaFabs are engineered therapeutic Fab fragments that can bind two targets simultaneouslyNature communicationsJanuary 29, 2021Beckmann R, Jensen K, Fenn S, Speck J, Krause K, Meier A, Röth M, Fauser S, Kimbung R, Logan DT, et al.

(8) Neointimal Hyperplasia after Carotid Transection and Anastomosis Surgery is Associated with Degradation of Decorin and Platelet Derived Growth Factor SignalingJVS- vascular scienceJanuary 1, 2021D'Cruz RJ, Sampson VB, Askinas CA, Scott RA, Robinson KG, Beaty CA, Hesek AM, Akins RE.(9101S) Anti-PDGFR-β,Rabbit,IgG,Unconjugated,Cell Signaling Technology (3169) Anti-hPDGF-BB,Goat,IgG,Unconjugated,R&D Systems (AF-220-NA) Anti-β-actin,Mouse,IgG1,Unconjugated,Sigma (A5441) B. Secondary antibodies,,,, Anti-sheep secondary,Donkey,IgG,HRP,ThermoFisher

(9) Identification of Emetine as a Therapeutic Agent for Pulmonary Arterial Hypertension: Novel Effects of an Old DrugArteriosclerosis, thrombosis, and vascular biologyNovember 1, 2019Siddique MAH, Satoh K, Kurosawa R, Kikuchi N, Elias-Al-Mamun M, Omura J, Satoh T, Nogi M, Sunamura S, Miyata S, et al.(bromodomain-containing protein 4; 2 µg/mL; Abcam), PDGF-BB (platelet-derived growth factor BB; 1 µg/mL; AF-220-NA; R&D systems), FGF2 (fibroblast growth factor-2; 1 µg/mL; Santa Cruz Biotechnology, Santa Cruz), HIF-1α

(10) Transforming Growth Factor-Beta Produced by Non-small Cell Lung Cancer Cells Contributes to Lung Fibroblast Contractile PhenotypeAnticancer researchApril 1, 2018Kanaji N, Yokohira M, Watanabe N, Kadowaki N, Bandoh S.was measured by ELISA. Briefly, 96-well plates were coated with monoclonal anti-PDGF-BB antibodies (AF-220-NA, R&D Systems, Minneapolis, MN, USA) overnight at 200 ng/100 μl each well. Next day, after rinsing with

(11) Harmine enhances type H vessel formation and prevents bone loss in ovariectomized miceTheranosticsJanuary 1, 2018Huang J, Yin H, Rao SS, Xie PL, Cao X, Rao T, Liu SY, Wang ZX, Cao J, Hu Y, et al.

(12) Discovery of pan-VEGF inhibitory peptides directed to the extracellular ligand-binding domains of the VEGF receptorsScience advancesOctober 1, 2016Michaloski JS, Redondo AR, Magalhães LS, Cambui CC, Giordano RJ.anti-human VEGF (AF-293-NA), anti-human VEGF-C (AF752), anti-human PlGF (AF-264-PB), anti-human PDGF-BB (AF-220-NA), anti-human FGF-basic (AF-233-NA), anti-mouse/rat NRP-1 (AF566), and anti-mouse/rat NRP-2 (AF567) were

(13) Treatment of refractory cutaneous ulcers with mixed sheets consisting of peripheral blood mononuclear cells and fibroblastsScientific reportsJune 22, 2016Ueno K, Takeuchi Y, Samura M, Tanaka Y, Nakamura T, Nishimoto A, Murata T, Hosoyama T, Hamano K.control antibody, anti-TGF-β1 antibody (38 μg/mL, ab64715, Abcam), or anti-PDGF-BB antibody (33 μg/mL, AF-220-NA, R&D Systems, Inc.) and then added to fibroblasts. The cell culture supernatant was harvested after

(14) Chemokine CCL15 Mediates Migration of Human Bone Marrow-Derived Mesenchymal Stem Cells Toward Hepatocellular CarcinomaStem CellsApril 1, 2016Gao Y, Zhou Z, Lu S, Huang X, Zhang C, Jiang R, Yao A, Sun B, Wang X.

(15) Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditionsPloS oneJanuary 1, 2013Smith AO, Bowers SL, Stratman AN, Davis GE.

(16) Rodent models of HAND and drug abuse: exogenous administration of viral protein(s) and cocaineJournal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune PharmacologyJune 1, 2012Yao H, Buch S.injection (i.p.) on days 3 and 5 at a concentration of 25 μg/mouse/injection (anti-PDGF-BB neutralizing Ab; AF-220-NA, or isotype control goat IgG1; AB-108-C, R&D systems). On the 8th day, animals were injected in the

(17) Naringenin inhibits neointimal hyperplasia following arterial reconstruction with interpositional vein graftAnnals of plastic surgeryJanuary 1, 2010Cayci C, Wahlquist TC, Seckin SI, Ozcan V, Tekinay AB, Martens TP, Oz MC, Ascherman JA.Immunohistochemical Staining Technique for PDGF-BB To determine the levels of PDGF-BB, we used goat antirhPDGF-BB antibody (AF-220-NA, R&D Systems, Minneapolis, MN) and a secondary antigoat IgG antibody. Slides were deparaffinized and

(18) VEGF-C regulates lymphangiogenesis and capillary stability by regulation of PDGF-BAmerican journal of physiology. Heart and circulatory physiologyNovember 1, 2009Onimaru M, Yonemitsu Y, Fujii T, Tanii M, Nakano T, Nakagawa K, Kohno R, Hasegawa M, Nishikawa S, Sueishi K.

(19) Inhibition of anastomotic intimal hyperplasia using a chimeric decoy strategy against NFkappaB and E2F in a rabbit modelCardiovascular researchSeptember 1, 2008Miyake T, Aoki M, Morishita R.

(20) A CXCL5- and bFGF-dependent effect of PDGF-B-activated fibroblasts in promoting trafficking and differentiation of bone marrow-derived mesenchymal stem cellsExperimental cell researchJuly 1, 2008Nedeau AE, Bauer RJ, Gallagher K, Chen H, Liu ZJ, Velazquez OC.

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PDGF-BB Antibody

PDGF-BB Antibody

PDGF-BB Antibody

PDGF-BB Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

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Reviews

PDGF-BB Antibody from R&D Systems, a Bio-Techne Brand

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