Human Phospho-VEGFR2/KDR/Flk-1 (Y1214) Antibody AF1766 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman Phospho-VEGFR2/KDR/Flk-1 (Y1214) Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $399.00Supplier Page View Company Product Page
Catalog NumberAF1766
ApplicationsTaqMan Protein Assay,Western Blot,Immunocytochemistry
ReactivityHamster,Human
IsotypeIgG
Quantity50 ug (also 25 ug)
HostRabbit
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
TargetVEGFR2/KDR/Flk-1
ClonalityPolyclonal
Modificationsp tyr1214
ImmunogenPhosphopeptide containing human VEGFR2/KDR/Flk-1 Y1214 site
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namekinase insert domain receptor
NCBI Gene AliasesCD309, FLK1, VEGFR, VEGFR2

Specifications/Features

Available conjugates: Unconjugated

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Selective Targeting of Tip Endothelial Cells as a Therapeutic Strategy for Tumor AngiogenesisAdvanced ScienceMarch 1, 2026Byoungmo Kim, Ha Kyeong Lee, Zulfikar Azam, Jeong Uk Choi, Riajul Wahab, Na Kyeong Lee, Yoon Gun Ko, So‐Young Choi, Se‐Ra Lee, Wan Seob Shim, et al.secondary antibody for 1 h. The following antibodies were used: Phospho‐VEGFR2 polyclonal antibody (1:2000, AF1766, R&D Systems), anti‐mouse VEGFR2 polyclonal antibody (1:1000, AF644, R&D Systems), anti‐human VEGFR2

(2) Vascular endothelial growth factor receptors 1 and 3 mediate placental trophoblast leptin production in preeclampsia, inducing vascular dysfunctionbioRxivJune 1, 2025Mona Elgazzaz, Safia Ogbi, Desmond Moronge, Elisabeth Mellott, Gibson Cooper, Kristin Backer, Joanna Hitchings, Luis Valesquez Zarate, Sravankumar Kavuri, Tae Jin Lee, et al.Cat# SAB4504006), anti-VEGFR1 (Abcam, Cat# ab32152), anti-phospho-VEGFR2 (pVEGFR2; R&D Systems,Cat# AF1766), antiVEGFR2 (Abcam, Cat# ab39638), anti-phospho-VEGFR3 (pVEGFR3; MyBioSource, Cat# MBS9612744), and

(3) Secretome of brain microvascular endothelial cells promotes endothelial barrier tightness and protects against hypoxia-induced vascular leakageMolecular medicine (Cambridge, Mass.)August 26, 2024Loiola RA, Hachani J, Duban-Deweer S, Sevin E, Bugno P, Kowalska A, Rizzi E, Shimizu F, Kanda T, Mysiorek C, et al.Target,Reference,Provider COX-2,AF4198,R&D systems Phospho-VEGFR2,AF1766,R&D systems Phospho-AKT,MAB887,R&D systems pan-VEGFR2,sc6251,SantaCruz Non-phospho(active)-β catenin,"19,807",CellSignalling Phospho-ERK1/2,9106,CellSignalling pan-ERK1/2,9102,CellSignalling pan-AKT,4691,CellSignalling pan-β

(4) Endocan Knockdown Down-Regulates the Expression of Angiogenesis-Associated Genes in Il-1ß Activated ChondrocytesBiomoleculesMay 18, 2023Scuruchi M, Aliquò F, Avenoso A, Mandraffino G, Vermiglio G, Minuti A, Campo S, Campo GM, D'Ascola A.

(5) Critical role of mitogen-inducible gene 6 in restraining endothelial cell permeability to maintain vascular homeostasisJournal of cell communication and signalingMarch 1, 2023Xing L, Huang G, Chen R, Huang L, Liu J, Ren X, Wang S, Kuang H, Kumar A, Kim JK, et al.pTyr951-VEGFR2 (2471, Cell Signaling), pTyr1054/59-VEGFR2 (44-1047G, Invitrogen), pTyr1214-VEGFR2 (AF1766, R&D Systems), VEGFR2 (9698, Cell Signaling), pTyr783-PLCγ1 (2821S, Cell Signaling), PLCγ1 (5690S, Cell Signaling

(6) Secretome of endothelial progenitor cells from stroke patients promotes endothelial barrier tightness and protects against hypoxia-induced vascular leakageStem cell research & therapyOctober 26, 2021Loiola RA, García-Gabilondo M, Grayston A, Bugno P, Kowalska A, Duban-Deweer S, Rizzi E, Hachani J, Sano Y, Shimizu F, et al.Target,Reference,Provider COX-2,AF4198,R&D Systems Phospho-VEGFR2,AF1766,R&D Systems Phospho-AKT,MAB887,R&D Systems pan-VEGFR2,sc6251,SantaCruz Non-phospho(active)-β catenin,"19,807",CellSignalling Phospho-ERK1/2,9106,CellSignalling pan-ERK1/2,9102,CellSignalling pan-AKT,4691,CellSignalling pan-β

(7) Ionizing radiation increases the endothelial permeability and the transendothelial migration of tumor cells through ADAM10-activation and subsequent degradation of VE-cadherinBMC cancerOctober 16, 2019Kouam PN, Rezniczek GA, Adamietz IA, Bühler H.used: P-β-catenin (Tyr142; diluted 1:500; #ab27798, abcam, Cambridge, UK); P-VEGF-R2 (Tyr1214; 1:1000, #AF1766, R&D Systems, Wiesbaden, Germany); VE-cadherin (BV9; 1:500; #sc-52,751, Santa Cruz Biotechnology, Heidelberg,

(8) SALM4 regulates angiogenic functions in endothelial cells through VEGFR2 phosphorylation at Tyr1175FASEB journal : official publication of the Federation of American Societies for Experimental BiologySeptember 1, 2019Kim DY, Park JA, Kim Y, Noh M, Park S, Lie E, Kim E, Kim YM, Kwon YG.(3366) were purchased from Cell Signaling Technology (Danvers, MA, USA). Phosphorylated VEGFR2-Y1214 (AF1766) was purchased from R&D Systems (Minneapolis, MN, USA). Phosphorylated eNOS (bs-3447R) was purchased

(9) Identification of Cross Talk between FoxM1 and RASSF1A as a Therapeutic Target of Colon CancerCancersFebruary 8, 2019Blanchard TG, Czinn SJ, Banerjee V, Sharda N, Bafford AC, Mubariz F, Morozov D, Passaniti A, Ahmed H, Banerjee A.(Y1213) (R and D Systems, #AF4170), p-VEGFR2 /KDR/Flk-1 (Y1214) (R and D Systems, Minneapolis, MN, USA, #AF1766), total VEGFR1 (R&D systems, #AF321), total VEGFR2 (R&D systems, #AF357), PTEN (R and D Systems, AF847),

(10) Upregulation of RASSF1A in Colon Cancer by Suppression of Angiogenesis Signaling and Akt ActivationCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacologyJanuary 1, 2018Blanchard TG, Lapidus R, Banerjee V, Bafford AC, Czinn SJ, Ahmed H, Banerjee A.Santa Cruz Biotechnology, Dallas, TX), phospho-VEGFR1/Flt-1 (Y1213) (AF4170), phospho-VEGFR2 /KDR/Flk-1 (Y1214) (AF1766, R&D systems), FOXM1 (R&D systems, Minneapolis, MN). Images were captured using a Syngene G Box digital

(11) Endothelial progenitor cells transplantation attenuated blood-brain barrier damage after ischemia in diabetic mice via HIF-1αStem cell research & therapyJuly 11, 2017Geng J, Wang L, Qu M, Song Y, Lin X, Chen Y, Mamtilahun M, Chen S, Zhang Z, Wang Y, et al.

(12) VEGF-A isoforms program differential VEGFR2 signal transduction, trafficking and proteolysisBiology openMay 15, 2016Fearnley GW, Smith GA, Abdul-Zani I, Yuldasheva N, Mughal NA, Homer-Vanniasinkam S, Kearney MT, Zachary IC, Tomlinson DC, Harrison MA, et al.factors Antibodies: goat-anti-VEGFR1 (#AF321), goat-anti-VEGFR2 (#AF357), rabbit-anti-phospho-VEGFR2-Y1214 (#AF1766) (R&D Systems, Minneapolis, MN, USA), rabbit-anti-Akt (#9272S), rabbit-anti-phospho-Akt (S473; #4060B),

(13) DSGOST inhibits tumor growth by blocking VEGF/VEGFR2-activated angiogenesisOncotargetApril 19, 2016Choi HS, Lee K, Kim MK, Lee KM, Shin YC, Cho SG, Ko SG.Millipore. α-tubulin (T5168) antibody was from Sigma (Thief River Falls, MN, USA). Phospho-VEGFR2 (Y1214) (AF1766) antibody was from R & D systems (Minneapolis, MN, USA). All antibodies were dilutions at 1:1000, tubulin

(14) Fluorescent resonance energy transfer imaging of VEGFR dimerizationAnticancer researchMay 1, 2014Ahmadova Z, Yagublu V, Förg T, Hajiyeva Y, Jesenofsky R, Hafner M, Keese M.

(15) Endothelial cell adhesion to soluble vascular endothelial growth factor receptor-1 triggers a cell dynamic and angiogenic phenotypeFASEB journal : official publication of the Federation of American Societies for Experimental BiologyFebruary 1, 2014Orecchia A, Mettouchi A, Uva P, Simon GC, Arcelli D, Avitabile S, Ragone G, Meneguzzi G, Pfenninger KH, Zambruno G, et al.

(16) Early VEGFR2 activation in response to flow is VEGF-dependent and mediated by MMP activityBiochemical and biophysical research communicationsMay 10, 2013dela Paz NG, Melchior B, Frangos JA.

(17) Vascular endothelial growth factor signals through platelet-derived growth factor receptor β in meningiomas in vitroBritish journal of cancerNovember 6, 2012Pfister C, Pfrommer H, Tatagiba MS, Roser F.(BAF293), KDR (BAF357), PDGFB (BAF220), and PDGFRβ (BAF385). Phospho-PDGFRβ (AF1767) and phospho-KSR (AF1766) were labelled with biotin using the Biotin-XX Microscale Protein Labelling Kit (Invitrogen, Carlsbad,

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Phospho-VEGFR2/KDR/Flk-1 (Y1214) Antibody

Phospho-VEGFR2/KDR/Flk-1 (Y1214) Antibody

Phospho-VEGFR2/KDR/Flk-1 (Y1214) Antibody

Phospho-VEGFR2/KDR/Flk-1 (Y1214) Antibody

Phospho-VEGFR2/KDR/Flk-1 (Y1214) Antibody

Human Phospho-VEGFR2/KDR/Flk-1 (Y1214) Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (17)

Reviews

Human Phospho-VEGFR2/KDR/Flk-1 (Y1214) Antibody from R&D Systems, a Bio-Techne Brand

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