Midkine Antibody AF-258-PB from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMidkine Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF-258-PB
NCBI Gene AliasesARAP, MK, NEGF2
ApplicationsImmunohistochemistry,Immunoprecipitation,Western Blot,Electron Microscopy,Simple Western,Immunocytochemistry, Functional Assay
TargetMidkine
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
ReactivityHuman,Mouse,Rat
HostGoat
Quantity100 ug (also 25 ug)
NCBI Full Gene Namemidkine
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human Midkine, Lys23-Asp143, Accession # P21741
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
ValidationKnockout Validated

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human Midkine in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse Midkine is observed, and less than 1% cross-reactivity with recombinant human Pleiotrophin is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Pan-neurodegeneration proteomics reveals disease subtypes and molecular signaturesCellMarch 23, 2026Him K. Shrestha, Huan Sun, Jay M. Yarbro, DongGeun Lee, Danting Liu, Erming Wang, Meghan McReynolds, Nan Zhang, Boer Xie, Shu Yang, et al.reagents,ThermoFisher Scientific,"Cat# A44520, A52045, 90406, A34808" MDK antibody,R&D Systems,Cat# AF-258-PB Aβ antibody,Immuno-Biological Laboratories,Cat# 10323 Software and Algorithms,, JUMP,"Wang et al.,

(2) A guide to selecting high-performing antibodies for human Midkine for use in Western blot and immunoprecipitationF1000ResearchAugust 1, 2024Riham Ayoubi, Kathleen Southern, Carl Laflamme, NeuroSGC/YCharOS Collaborative Groupapplications GeneTex,GTX108439,39855,AB_1950903,polyclonal,-,rabbit,0.33,Wb GeneTex,GTX116089 a,40597,AB_11165850,polyclonal,-,rabbit,1.00,Wb Bio-Techne,AF-258-PB,WE0519091,AB_2143400,polyclonal,-,goat,0.20,Wb Bio-Techne,MAB2582 **,CMHR0219071,AB_2893288,recombinant-mono,1011522,mouse,0.50,IF Bio-Techne,MAB2583

(3) A guide to selecting high-performing antibodies for human Midkine for use in Western blot and immunoprecipitationF1000ResearchJanuary 1, 2023Ayoubi R, Southern K, Laflamme C, NeuroSGC/YCharOS Collaborative Group.applications GeneTex,GTX108439,39855,AB_1950903,polyclonal,-,rabbit,0.33,Wb GeneTex,GTX116089 a,40597,AB_11165850,polyclonal,-,rabbit,1.00,Wb Bio-Techne,AF-258-PB,WE0519091,AB_2143400,polyclonal,-,goat,0.20,Wb Bio-Techne,MAB2582 **,CMHR0219071,AB_2893288,recombinant-mono,1011522,mouse,0.50,IF Bio-Techne,MAB2583

(4) Significance of serglycin and its binding partners in autocrine promotion of metastasis in esophageal cancerTheranosticsJanuary 1, 2021Zhu Y, Lam AKY, Shum DKY, Cui D, Zhang J, Yan DD, Li B, Xu WW, Lee NPY, Chan KT, et al.antibodies included mouse anti-SRGN (#sc-393521, Santa Cruz Biotechnology, Dallas, TX, USA), goat anti-MDK (#AF-258-PB, R&D Systems, Inc., Minneapolis, MN, USA), rabbit anti-MMP2 (#ab92536, Abcam, Cambridge, United Kingdom)

(5) Midkine Is a Potential Therapeutic Target of Tumorigenesis, Angiogenesis, and Metastasis in Non-Small Cell Lung CancerCancersAugust 24, 2020Shin DH, Jo JY, Kim SH, Choi M, Han C, Choi BK, Kim SS.

(6) Deep Multilayer Brain Proteomics Identifies Molecular Networks in Alzheimer's Disease ProgressionNeuronMarch 18, 2020Bai B, Wang X, Li Y, Chen PC, Yu K, Dey KK, Yarbro JM, Han X, Lutz BM, Rao S, et al.diluted with PBST plus 2% BSA and 0.2% skim milk (PBST-B) as follow: MDK (1:100, R&D Systems, Cat# AF-258-PB), NTN1 (1:100, Abcam, ab126729), human SMOC1 (1:50, MilliporeSigma, Cat# SAB2701398), mouse SMOC1 (1:50,

(7) Development of bead based multiplexed immunoassay for evaluation of midkine, syndecan-1, and ANGPTL4 in patient serumJournal of immunoassay & immunochemistryJanuary 1, 2018Tarhoni I, Fhied CL, Pool M, Liptay MJ, Bonomi P, Seder CW, Borgia JA.R&D Systems (Minneapolis, MN), which included the following: “capture” antibodies—Human Midkine Antibody (AF-258-PB), Human Syndecan-1/CD138 Antibody (AF2780), and ANGPTL4 (AF3485); recombinant proteins (as standards)—Midkine

(8) Role of heparin binding growth factors in nigrostriatal dopamine system development and Parkinson's diseaseBrain researchMay 25, 2007Marchionini DM, Lehrmann E, Chu Y, He B, Sortwell CE, Becker KG, Freed WJ, Kordower JH, Collier TJ.

(9) Regulation of MDK expression in human cancer cells modulates sensitivities to various anticancer drugs: MDK overexpression confers to a multi-drug resistanceCancer lettersMarch 8, 2007Kang HC, Kim IJ, Park HW, Jang SG, Ahn SA, Yoon SN, Chang HJ, Yoo BC, Park JG.

(10) Neuritogenic activity of chondroitin/dermatan sulfate hybrid chains of embryonic pig brain and their mimicry from shark liver. Involvement of the pleiotrophin and hepatocyte growth factor signaling pathwaysThe Journal of biological chemistryFebruary 2, 2007Li F, Shetty AK, Sugahara K.

(11) The anti-HIV cytokine midkine binds the cell surface-expressed nucleolin as a low affinity receptorThe Journal of biological chemistryOctober 4, 2002Said EA, Krust B, Nisole S, Svab J, Briand JP, Hovanessian AG.

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Midkine Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (11)

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Midkine Antibody from R&D Systems, a Bio-Techne Brand

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