B7-H4 Antibody AF2154 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemB7-H4 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF2154
NCBI Gene AliasesB7H4, PRO1291, B7X, B7-H4, B7h.5, B7x, B7s1, B7S1, B7h4, VCTN1
ApplicationsFlow Cytometry,Immunohistochemistry,Western Blot,CyTOF-ready
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
HostGoat
Quantity100 ug (also 25 ug)
ReactivityHuman,Mouse,Transgenic Mouse
TargetB7-H4
Conjugate/TagUnconjugated
NCBI Full Gene NameV-set domain containing T cell activation inhibitor 1
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse B7-H4, Phe29-Pro258, Accession # Q7TSP5
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse B7-H4 in direct ELISAs and Western blots. Detects human and mouse B7-H4 in Western blots.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used for detecting B7-H4 in both human and mouse samples?

Yes, the B7-H4 Antibody has been shown to detect human and mouse B7-H4 in Western blots, making it suitable for applications involving both species.

What applications is this antibody suitable for in my research?

The B7-H4 Antibody is suitable for use in Western blotting, flow cytometry, immunohistochemistry, and multiplex assays, providing versatility for various experimental setups.

Is there any specific information on the immunogen used for this antibody?

The B7-H4 Antibody was generated using a recombinant mouse B7-H4 protein derived from the mouse myeloma cell line NS0, specifically the region from Phe29 to Pro258, which ensures specificity for the target.

How should I store this antibody after reconstitution to maintain its stability?

After reconstitution, the antibody should be stored under sterile conditions at -20 to -70 °C for up to six months. It can also be kept at 2 to 8 °C for one month, but repeated freeze-thaw cycles should be avoided to maintain its integrity.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) The immune checkpoint molecule B7-H4 regulates β-cell mass and insulin secretion by modulating cholesterol metabolism through Stat5 signallingMolecular MetabolismJanuary 1, 2025Fangzhen Xia, Ziteng Zhang, Zhen Qian, Xiaoyu Fang, Junxue Wang, Yan Wang, Guoting Sun, Yuefeng Yu, Ninjian Wang, Junke Zhen, et al.Extraction Kit (Solarbio,R0050). Primary antibodies were as follows: anti-B7-H4 (1:500, R&D Systems, AF2154), anti-Apof (1:250; Abcam, ab231585), anti-Stat5 (1:1,000; Cell Signaling Technology, 94205), anti-phospho-Stat5 (Tyr694

(2) Pancreatic Epithelial IL17/IL17RA Signaling Drives B7-H4 Expression to Promote TumorigenesisCancer immunology researchSeptember 3, 2024Castro-Pando S, Howell RM, Li L, Mascaro M, Faraoni EY, Le Roux O, Romanin D, Tahan V, Riquelme E, Zhang Y, et al.Section. We used anti-IL17-RA (clone H-168, Rabbit, Santa Cruz), anti-B7-H4 (R&D Systems Catalog # AF2154), anti-SMA (clone 1A4, Thermo Fisher Scientific), and anti-E-cadherin (Alexa Fluor 488, clone 36, BD

(3) Epithelial Expressed B7-H4 Drives Differential Immunotherapy Response in Murine and Human Breast CancerCancer Res CommunApril 24, 2024Wescott EC, Sun X, Gonzalez-Ericsson P, Hanna A, Taylor BC, Sanchez V, Bronzini J, Opalenik SR, Sanders ME, Wulfkuhle J, et al.blocked and protein block was applied. Sections were then incubated with the primary antibodies (B7-H4 AF2154 R&D Systems at 1:600, CD45 ab10558 Abcam at 1:2,500, B7-H4 D1M8I Cell Signaling Technology 1:200, pan-Cytokeratin

(4) Molecular Pathways: Evaluating the Potential for B7-H4 as an Immunoregulatory TargetClinical cancer research : an official journal of the American Association for Cancer ResearchJune 15, 2017MacGregor HL, Ohashi PS.

(5) Loss of Peripheral Protection in Pancreatic Islets by Proteolysis-Driven Impairment of VTCN1 (B7-H4) Presentation Is Associated with the Development of Autoimmune DiabetesJournal of immunology (Baltimore, Md. : 1950)February 15, 2016Radichev IA, Maneva-Radicheva LV, Amatya C, Salehi M, Parker C, Ellefson J, Burn P, Savinov AY.

(6) B7-H3 and B7x are highly expressed in human prostate cancer and associated with disease spread and poor outcomeProceedings of the National Academy of Sciences of the United States of AmericaDecember 4, 2007Zang X, Thompson RH, Al-Ahmadie HA, Serio AM, Reuter VE, Eastham JA, Scardino PT, Sharma P, Allison JP.

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B7-H4 Antibody

B7-H4 Antibody

B7-H4 Antibody

B7-H4 Antibody

B7-H4 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (6)

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B7-H4 Antibody from R&D Systems, a Bio-Techne Brand

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