Fig 1: APOSECH induces cytoprotective protein expression and activation. a Cell extracts were prepared after stimulation with APOSECH or control medium after the indicated time intervals. Western blot analysis showed activation of c-Jun, CREB, AKT, ERK1/2 and Hsp27. b shows the dose-dependent induction of Hsp27 and CREB in cultured cardiomyocytes. c 24 h after treatment expression of the anti-apoptotic proteins Bcl-2 and BAG1 was analyzed by Western blotting. Proteins were normalized to the house-keeping gene GAPDH. One representative experiment of two is shown. d shows a dose-dependent cytoprotective effect of APOSEC in a starvation induced cell death model in cardiac myocytes. e shows that neutralization of selected factors (VEGF, IL-8, ENA-78 and MMP9) does not attenuate the induction of CREB and Bcl-2 in cardiomyocytes by APOSEC co-incubation