Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF-7 (Lane 1), T-47D (Lane 2), MDA-MB-231 (Lane 3), Hep G2 (Lane 4), Jurkat (Lane 5), K-562 (Lane 6), HeLa (Lane 7), U-87 MG (Lane 8) and IMR32 (Lane 9). The blots were probed with Anti-SOD1 Recombinant Rabbit Polyclonal Antibody (Product # 711818, 1 µg/mL). A 16 kDa band corresponding to SOD1 was observed across the cell lines tested. The blots were detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12% Bis-Tris gel (Product # NP0341BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).