Fig 1: ZBTB28 upregulated FIP200 to induce autophagy and apoptosis. a qRT-PCR results of FIP200 mRNA expression in stable transfected cervical cancer cells. b Structure of wild-type and mutant FIP200 reporter plasmid. c % Input of FIP200 DNA by HA-tag antibody were tested by ChIP-PCR. Then products of ChIP-PCR were used for electrophoresis. d The effect of ZBTB28 on FIP200 promoter activity was detected by dual luciferase reporter system. e CaSki and HeLa cells were transfected with pcDNA3.1 or ZBTB28, then tested promoter luciferase activity of mutant FIP200 in live cells. f Forty eight hours after transfection with siFIP200 or control siRNA, the expression of FIP200, p62, LC3, Cleaved-casp8, Casp3, Cleaved-casp3 and Cleaved-PARP were evaluated by western blot. ß-actin was used as negative control. g Stable transfected CaSki and HeLa cells were re-transfected with siFIP200 or control siRNA. Seventy two hours after transfection, cell viability was assessed by CCK8 assay. *p < 0. 05, **p < 0. 01, ***p < 0. 001