Fig 1: Formation of cyclin/CDK/cyclin-dependent kinase inhibitor (CKI) complexes during liver injury. (A) Extracts from liver tissue were subjected to immunoprecipitation and Western analysis. Association of p21 with cyclin D1, CDK4, and CDK2; association of cyclin D1 with CDK4 and p21; association of p27 with cyclin D1 and CDK4. (B) Immunoblots on total liver lysates from 2 samples of each group for phospho-RB (pRB) were performed.