PCNA Antibody (PC10)

Product Specs
ItemPCNA Antibody (PC10) - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $479.00Supplier Page View Company Product Page
Catalog NumberNB500-106
Concentration1 mg/ml
Quantity0.1 ml (also 0.025 ml)
TargetPCNA
HostMouse
ApplicationsChemotaxis,IF/IHC,Chromatin Immunoprecipitation,Flow Cytometry,ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunohistochemistry Free-Floating,Simple Western,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry/ Immunofluorescence
ClonePC10
ClonalityMonoclonal
IsotypeIgG2a Kappa
Conjugate/TagUnconjugated
FormatPBS
ReactivityAvian - Chicken,Chicken,Drosophila,Fish,Human,Marsupial,Mouse,Porcine,Primate,Rabbit,Rat,Yeast,Zebrafish,Fish - Danio rerio (Zebrafish)
PurityProtein A purified
Molecular Weight30 kDa
Research AreaAutophagy, Base Excision Repair, Cellular Markers, DNA Repair, Loading Controls
Antibody TypePrimary Antibodies
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles
NCBI Full Gene Nameproliferating cell nuclear antigen
NCBI Gene AliasesATLD2
ImmunogenProtein A-rat PCNA fusion obtained from pC2T

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Specific for PCNA p36 protein expressed at high levels in proliferating cells.

Sizes Available: 0.1 ml (also 0.025 ml)

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Questions and Answers

Can this antibody be used to study cell proliferation in various species?

Yes, the PCNA antibody (PC10) has been shown to work effectively in multiple species including humans, mice, rats, zebrafish, and other mammals, as well as in Drosophila and yeast. Its specificity for the proliferating cell nuclear antigen makes it suitable for assessing cell proliferation across these diverse organisms.

How has this antibody been utilized in recent research?

The PCNA antibody has been employed in various studies, such as in the investigation of alpha-synuclein pathology and its effects on neurogenesis in a mouse model of Parkinson's disease, where it was used for immunohistochemistry to quantify neural progenitor cells (Bender et al., 2021). Additionally, it has been used to assess the effects of human umbilical cord-derived mesenchymal stem cells on wound healing in diabetic rats (Chen et al., 2023).

What are the recommended applications for this antibody based on scientific literature?

The PCNA antibody is recommended for applications including Western blotting, immunohistochemistry, and immunofluorescence, as evidenced by its use in studies examining cellular responses to DNA damage and proliferation in various experimental models (Chung et al., 2021; Rossi et al., 2021).

Is there any evidence of this antibody's effectiveness in specific experimental conditions?

Yes, the PCNA antibody has been shown to effectively detect proliferating cells in various experimental conditions, such as in the assessment of corneal epithelial recovery in a rabbit model (Chung et al., 2021) and in evaluating the effects of graphene on brain development in chicken embryos (Chwalibo et al., 2014). These studies highlight its utility in different biological contexts.

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

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(10) Toxicity of pristine graphene in experiments in a chicken embryo modelInternational Journal of NanomedicineAugust 26, 2014André Chwalibog, Slawomir Jaworski, Slawomir Jaworski, Marta Kutwin, Mateusz Wierzbicki, Mateusz Wierzbicki, Natalia Kurantowicz, Natalia Kurantowicz, Barbara Lipińska, Ludwika Chwaliboghttps://www.dovepress.com/ by 135.181.162.250 on 16-Nov-2021 For personal use only. Sawosz et al PCNA (NB500-106; Novus Biologicals, Littleton, CO, USA). Sections for this purpose were incubated with mouse monoclonal

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