Phospho-NF-kappaB p65 (Ser536) (93H1) Rabbit mAb 3033L from Cell Signaling Technology

Product Specs
ItemPhospho-NF-kappaB p65 (Ser536) (93H1) Rabbit mAb
CompanyCell Signaling Technology
Price $947.00 Inquire
Catalog Number3033L
Quantity300 µl
ApplicationsW, W-F, W-S, IP, IF-IC, FC-FP
ReactivityH, M, R, Hm, Mk, Pg
TargetNFkB-p65 (Ser536) phosphate
ModificationsNFkB-p65 (Ser536) phosphate
HostRabbit
NCBI Gene AliasesAIF3BL3, CMCU, NFKB3, p65
ClonalityMonoclonal
NCBI Full Gene NameRELA proto-oncogene, NF-kB subunit
Research AreaImmunology and Inflammation
IsotypeIgG
Clone93H1
Molecular Weight65

Questions and Answers

Can this antibody be used to study NF-κB signaling in cancer models?

Yes, the Phospho-NF-κB p65 (Ser536) Rabbit mAb has been utilized in studies examining NF-κB signaling pathways in various cancer models, including its role in pancreatic cancer as demonstrated in the research by Cheung et al. (2020), where NF-κB activation was linked to tumor progression and metastasis.

Is this antibody effective for detecting NF-κB p65 phosphorylation in different species?

The antibody has been shown to react with multiple species, including human, mouse, rat, canine, non-human primate, hamster, and porcine. This broad reactivity allows for its application in comparative studies across different animal models.

How does this antibody perform in immunofluorescence applications?

The Phospho-NF-κB p65 (Ser536) Rabbit mAb has been successfully used in immunofluorescence applications, allowing researchers to visualize the localization and activation state of NF-κB p65 in cellular contexts, which is crucial for understanding its role in inflammation and cancer.

Are there any specific pathways or mechanisms that this antibody has been associated with in published studies?

Yes, this antibody has been associated with the RANKL-induced NF-κB pathway in studies investigating osteoclastogenesis, as shown in the work by Ju et al. (2021), where the inhibition of this pathway was linked to reduced osteoclast differentiation and bone pain in cancer models.

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Website: Technical Support

(1) COPB2 drives gastric cancer progression via PI3K/AKT/NF-κB signaling: a multi-omics and functional studyCell Adhesion & MigrationDecember 31, 2026Hailong Li, Dong Wei, Xiaqing Gao, Rong Su, Chunting Yang, Ping Tang, Xiqiu Yu, Yuhong Wuproteins such as p-AKT (1:1000, #4060, CST), p-mTOR (1:1000, ab109268, ABCAM), and p-NFκB-p65 (1:1000, 3033S, CST) were also detected via western blotting. The experiments were performed in triplicate, with GAPDH

(2) Gut-derived genistein from Parabacteroides distasonis alleviates psoriatic inflammation via CD200-mediated NF-κB inhibition in miceGut MicrobesDecember 31, 2026Demengjie Le, Mohammad Hassan Heidari, Shunmin Zhu, Yingying Luo, Changzhou Li, Yue Li, Bin Yang, Xuan WangDF10155, Affinity), anti-p-65 (1:1000, 8242 T, Cell Signaling Technology, USA), anti-p-p65 (1:1000, 3033 T, Cell Signaling Technology, USA), anti-IKBα (1:1000, 61294S, Cell Signaling Technology, USA), and

(3) A BAI1-PSTB-Hydrogel promotes diabetic wound healing by targeting mtDNA leakage and the cGAS–STING axis to alleviate endothelial senescenceBioactive MaterialsNovember 1, 2026Xianqi Wang, Rui Zheng, Wenjie Cheng, Yanan Xu, Xian-jie Xu, Heliang Fu, Dan Wu, Peiwen Wang, Yang Zhang, Huirong Wu, et al.13647S), p-TBK1 (CST, 5483S), TBK1 (CST, 38066S), p-IκB (CST, 2859S), IκB (CST, 4812S), p-NF-κB (CST, 3033S), NF-κB (Proteintech, 80979-1-RR), p-IRF-3 (CST, 2859S), IRF-3 (CST, 4812S). After washing, membranes

(4) Multi-omics identifies VMP1 as a tumor-intrinsic biomarker associated with metastatic progression and immune microenvironment remodeling in HNSCCTranslational OncologySeptember 1, 2026Xinhua Li, Ailin Yang, Leidong Yue, Chao Jing, Xudong Wanganti-VMP1 (#12,929; WB 1:1000, IHC 1:200), anti-p65 (#8242; WB 1:1000), and anti-phospho-p65 (p-p65, #3033; WB 1:1000). Antibodies from Proteintech (Rosemont, IL, USA; Wuhan, China) included anti-E-cadherin

(5) Therapeutic Lithium Enhances Klotho Expression and Mitochondrial Size and Branching in Human Cardiac Fibroblasts Involving Nuclear Factor-kappa B InhibitionClinical Psychopharmacology and NeuroscienceAugust 31, 2026Pao-Huan Chen, Ting-Wei Lee, Shuen-Hsin Liu, Tzu-Yu Cheng, Yu-Hsun Kao, Yi-Jen Chen(monoclonal; #8242, Cell Signaling Technology), and phosphorylated NF-κB p65 1:2,000 (monoclonal; #3033, Cell Signaling Technology). Bound antibodies were detected using an enhanced Chemiluminescence detection

(6) Pseudorabies virus pUL40 drives inflammatory signaling through competitive hijacking of EphA2 from the Akt-EphA2 interactionVet ResAugust 25, 2026Yutong Tian, Hang Yin, Jiaxiang Fu, Qingqing Yang, Ru Yan, Yuqing Li, Chao Ye, Rendong Fang(4303), anti-Phospho-SAPK/JNK (Thr183/Tyr185) (4668), NF-κB p65 (8242), Phospho-NF-κB p65 (Ser536) (3033), p44/42 MAPK (Erk1/2) (4695) and Phospho-p44/42 MAPK (Erk1) (Tyr204)/(Erk2) (Tyr187) (5726) antibodies

(7) Innate immune sensor NOD2 promotes cartilage degradation and osteoarthritis progression by stabilizing TRAF6 in chondrocytesJCI InsightAugust 24, 2026Yuting Wang, Song Li, Yonghui Dong, Jiaming Zhang, Jian Liu, Zhenggang Wang, Shuang Liang, Nathan R. Martinez, Hongxu Pu, Peng Cheng, et al.p-TAK1 (catalog 4508), IKK (catalog 8943), p-IKK (catalog 2697), P65 (catalog 8242), p-P65 (catalog 3033), AKT (catalog 4691), p-AKT (catalog 4060), JNK (catalog 9252), p-JNK (catalog 4668), P38 (catalog 8690),

(8) ADAR1 regulates radiosensitivity in ESCC through cIAP2-NF-κB signaling and C16orf46 editingiScienceAugust 21, 2026Liangchao Sun, Ahmed Ghanim Glayl, Ming Zhang, Chenglong Zhao, Jialin Sun, Kaikai Zhao, Jiaxin Sun, Xue MengAB_3674098 Phospho-NF-kappaB p65 (Ser536) (93H1) Rabbit Monoclonal Antibody,Cell Signaling Technology,Cat#3033 FLAG (DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope),Proteintech,RRID: AB_2918475 GAPDH Mouse mAb (High Dilution

(9) Early targeting of the YAP/TEAD-PKC-NF-κB axis alleviates post-traumatic osteoarthritisiScienceAugust 21, 2026Quinn T. Ehlen, Ying Wang, Xiaomin Cai, Sebastian Thompson-Ceccato, Thomas Jacob Jahn, Joshua H. Wu, Adair Treadway, Aliya Mulati, Lee Kaplan, Kiran Kurmi, et al.(D14E12),Cell Signaling Technology,#8242; RRID: AB_10859369 pS536 NFκB-p65,Cell Signaling Technology,#3033; RRID: AB_331284 YAP(/TAZ) (63.7),Santa Cruz Biotechnology,#sc-101199; RRID: AB_1131430 YAP/TAZ (E9M8G),Cell Signaling Technology

(10) Dual roles of SBSN in renal cancer progression and tumor thrombus: Cell-autonomous NF-κB-CD44 axis and IFI6-driven paracrine angiogenesisiScienceAugust 21, 2026Yuedian Ye, Jinbin Xu, Weihao Liu, Zhansen Huang, Xiaoming Li, Jiang Li, Yuanpeng Liao, Sam Un Cheong, Zifeng Xu, Gengguo Deng, et al.Primary antibodies included anti-SBSN (ab232771) (Abcam), anti-NF-κB p65 (#8242), anti-p-NF-κB p65 (#3033), cleaved caspase-3 (#9661), IFI6 (#54355), Bax (#2772), CD44 (#3578), p-IKKα/β (Ser176/180) (#2697),

(11) NF1 Leucine Rich Domain-Derived Extracellular Vesicles Remodel the Glioblastoma Immune Microenvironment via an ADAM17-Associated Inflammatory ProgramJ Extracell VesiclesAugust 21, 2026Yuganthini Vijayanathan, Jacqueline Yt Yeo, Janice Hy Tan, Fikri Bin Mohamed, Bernett Lee, Tatsuya Kozaki, Nurashikin Abdul Halim, Rachel Ly Ho, Hai Tao Tu, Zhi Wei Zhang, et al.Inc., Danvers, MA), GM130 (B‐10) (sc55591, 1:100 dilution, Santa Cruz Biotechnology), phospho‐p65 (3033, 1:500 dilution, Cell Signaling Technology Inc), p65 (8242, 1:1000 dilution, Cell Signaling Technology

(12) The role of NLRP3 inflammasome in the development of postoperative ileus and its molecular mechanism in male micePhysiol RepAugust 20, 2026Fan Zhang, Beicheng Sun, Hongqian Guocaspase‐1, 1:800, Cell Signaling Technology, #89332; p‐NF‐κB p65, 1:1000, Cell Signaling Technology, #3033; GAPDH, 1:1000, Proteintech, #60004‐1‐Ig). The membrane was then washed three times with TBST and incubated

(13) Network Pharmacology-Metabolomics Integration Reveals the Multiple Targets and Mechanisms of Total Glucosides of Paeony Against Ulcerative ColitisJ Inflamm ResAugust 19, 2026Yifei Wang, Yuxin Han, Fei Teng, Yi Liu, Liangjie Ma, Wenqi Ji, Xiao MaChina), Lipopolysaccharides (LPS) (L8880, Solarbio, China), Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (3033S, Cell Signaling Technology, USA). Primer sequences for PCR amplification are shown in Table 1, which

(14) Targeting FAR1 to inhibit intestinal macrophage ferroptosis: repurposing irinotecan as a novel sepsis therapyApoptosisAugust 19, 2026Haoyue Deng, Weifei Wang, Qinghui Li, Xinming Xiang, Yu Zhu, Zisen Zhang, Xiaowei Zhou, Yue Wu, Jie Zhang, Liangming Liu, et al.(Centennial, CO, USA); GSDMD-NT(1:1000; 10137T), p-MLKL(1:1000; 37333T), MLKL(1:1000; 37705T), p-p65(1:1000; 3033T), and p65(1:1000; 8242T) from Cell Signaling Technology (Danvers, MA, USA); cleaved Caspase-3(1:1000;

(15) Microglial Deubiquitinase OTUD7B Stabilizes STAT3 to Promote Neuroinflammation and Cognitive Decline in Alzheimer's DiseaseAdv Sci (Weinh)August 19, 2026Luyao Li, Hao Tang, Qin Yu, Yuyang Zhang, Binglu Shi, Ying Kong, Leiyu Xu, Jingjing Shao, Chenghong Hu, Lingyu She, et al.(Cat#: 1507), His‐tag (Cat#: 2365), Flag‐tag (Cat#: 14793), NF‐κB p65 (Cat#: 824), p‐NF‐κB p65 (Cat#: 3033), AlexaFluor594 (Cat#: 8889), and AlexaFluor488 (Cat#: 4412) were purchased from Cell Signaling Technology

(16) Real-Time Scanning Ion Conductance Microscopy Reveals MARCKS-Driven Morphological Remodeling and Nanomechanical Softening during LPS-Induced Macrophage PolarizationAnal ChemAugust 18, 2026Dong Wang, Ritsuko Yamase, Kyunghee Park, Hiromi Nishikawa, Andrew Shevchuk, Rebecca C. Coll, Yuri Korchev, Wei Wang, Yanjun Zhang(Cell Signaling Technology, #8722T), iNos (Millipore, AB5382), phospho- NF-κB-p65 (Cell Signaling Technology, #3033) and total NF-κB p65 (Cell Signaling Technology, #8242) were used as primary antibodies. Anti-GAPDH

(17) Esketamine Alleviates Neuropathic Pain and Inflammation via miR-153-3p/AKT3 in CCI RatsPain Res ManagAugust 17, 2026Xiumei Zhu, Yawen Zhangdilute specific primary antibodies of AKT3 (CST,4059), p‐IKKα/β (Santa Cruz, sc‐23470‐R), p‐p65 (CST, 3033), p65 (Proteintech, 10745‐1‐AP), iNOS (CST, 13,120), COX2 (CST, 4842), and β‐actin (Abcam, ab8226).

(18) Chronic Hypoxia-Induced Reprogramming of Smooth Muscle Cells: Insights into Transcription Factors, Cellular Stresses, and Therapeutic ImplicationsBiomedicinesAugust 16, 2026Sheng-Huei Wang, Yuan-Ming Tsai, Shu-Ting Liu, Shih-Ming Huang#9715, 1:3000 dilution), LC3B (catalog no.: #2775, 1:1000 dilution), and p-NFκB-p65 (S536) (catalog no.: 3033, 1:1000 dilution) were obtained from Cell Signaling Technology (Danvers, MA, USA). SOD1 (catalog no.:

(19) ε-Viniferin Attenuates LPS-Induced Inflammatory Activation in Canine Macrophages by Regulating NF-κB and MAPK PathwaysPrev Nutr Food SciAugust 14, 2026Minseo Cho, Jisu Lee, Hyunsoo Jang, Hyeon-Cheol Cha, Gisol Kim, Jaeho Pyee, Bong-Hyun Kim, Su-Cheol Han, Jeong Ho Hwang, Jung-Heun Haantibody,p-JNK,4668,1:500,CST Primary antibody,t-JNK,9252,"1:1,000",CST Primary antibody,p-NF-κB,3033,"1:1,000",CST Primary antibody,t-NF-κB,8242,"1:1,000",CST Primary antibody,p-IκBα,2859,"1:1,000",CST Primary

(20) α‑Cyperone targets the PAK1‑JNK/NF‑κB axis to modulate dendritic cells maturation and Th2/Th9 differentiation in an asthma modelInt J Mol MedAugust 14, 2026Hang Hu, Xing Wang, Linlin Guo, Ziling Zeng, Hongmei Tang, Yun Zhang, Jiayao Duan, Kaijin WangAntibodies obtained from Cell Signaling Technology, Inc.: phosphorylated (p-) NF-κB (1:1,000; cat. no. 3033), NF-κB (1:1,000; cat. no. 8242), p-JNK (1:1,000; cat. no. 4668S), JNK (1:1,000; cat. no. 9252S), p-c-Jun

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8/31/2022Anti-p-S536-NFkB p65 Antibody Works Very Well Liyong Z.BT20 cells were treated with 30 ng/ml TNFalpha for 30 minutes and p-S536-NFkB p65 antibody was examined by Western blot to check the response of NFkB signaling pathway. The results showed that this ... Read Review
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7/22/2022Phospho-NF-KB p65 (Ser536) (93H1) mAb for Western Blot Ines RThis antibody was used to detect NF-?B p65 levels (only when phosphorylated at Ser536) from human cell lysates (25 ug/mL protein loading) through western blot analysis.
(1)

1/25/2021Analysis of Changes in pp65 Expression Lisha JoshiIn our experimental project we determined downstream signaling pathways that are activated by lysophosphatidic acid (LPA). We investigated the effect of LPA on inflammatory transcription factors in ... Read Review
(1)

5/16/2019Phospho-NF-kB p65 Antibody Great for Western Blotting from CST Ab GUse this antibody for a detection of phospho NF-kB in various human cancers with ease!
(1)

5/15/2015Fantastic p-p65 antibody TY CWe study NF-kB activation by monitoring p-p65 over time in bone marrow macrophages upon LPS stimulation by Western Blot. This antibody has been consistently reliable from Day 1, and detects in milk ... Read Review

Phospho-NF-kappaB p65 (Ser536) (93H1) Rabbit mAb

Phospho-NF-kappaB p65 (Ser536) (93H1) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Phospho-NF-kappaB p65 (Ser536) (93H1) Rabbit mAb from Cell Signaling Technology

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