RAT ANTI TUBULIN ALPHA MCA77G from Bio-Rad (AbD Serotec - FAB)

Product Specs
ItemRAT ANTI TUBULIN ALPHA
CompanyBio-Rad
Price $594.00Supplier Page View Company Product Page
Catalog NumberMCA77G
TargetTUBULIN ALPHA
CloneYL1/2
ImmunogenYeast tubulin
ApplicationsWestern Blotting,Immunofluorescence,Immunoprecipitation,Immunohistology - Frozen,ELISA,Radioimmunoassays
Quantity0.5 mg
IsotypeIgG2a
HostRat
FormatPurified IgG - liquid
ReactivityYeast, Ashbya,Human,Mouse,Dog,Rat,Pig,Drosophila,Saccharomyces,Pleurobrachia,Caenorhabditis,Dictyostelium discoideum,Xenopus,Pig-tailed macaque,Clytia sp.,Arabidopsis,Strongylocentrotus purpuratus,Dendraster excentricus,Trypanosoma brucei,Potorous tridactylis,Bovine,Hemicentrotus pulcherrimus,Potato,Bombyx mori,Rhodnius prolixus,Beroe abyssicola,Candida sp
ClonalityMonoclonal Antibody
ConcentrationIgG concentration 1.0 mg/ml

Questions and Answers

What applications has the rat anti-tubulin antibody been used for in scientific research?

The rat anti-tubulin antibody has been shown to work in various applications including Western Blotting, ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Radioimmunoassay.

Can this antibody be used for immunofluorescence microscopy, and if so, what are the recommended dilution and conditions?

Yes, the rat anti-tubulin antibody (MCA77G) has been utilized in immunofluorescence microscopy at a dilution of 1:500 to 1:2000, depending on the specific experimental setup. It has been effectively used to visualize microtubules in various cell types.

Is there any evidence of this antibody being used in studies related to cancer research?

Yes, the rat anti-tubulin antibody has been cited in studies such as those investigating the cytotoxic effects of GSK-3 inhibition in glioma stem cells, where it was used to assess microtubule dynamics and centrosome function during cell division ().

What species does this antibody react with, and how broad is its reactivity?

The rat anti-tubulin antibody exhibits broad reactivity, being effective in detecting tubulin in multiple species including human, mouse, rat, various avian and mammalian species, as well as invertebrates and plants. This wide range makes it suitable for diverse biological studies.

Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Chromosome condensation mechanically primes the nucleus for mitosisThe EMBO JournalJune 29, 2026Vanessa Nunes, Margarida Moura, Sara F Silva, Débora Vareiro, Nicolas Audugé, Nicolas Borghi, Jorge G Ferreira2010", Antibodies,Antibodies,Antibodies Mouse anti-Lamin A/C,Abcam,4C11 Rabbit anti-Lamin B1,Abcam,ab16048 Rat anti-alpha tubulin,Bio-Rad,MCA77G Rabbit anti-NudE/NudEL antibody,"Vallee Lab (Stehman et al, 2007)", Rabbit anti-BicD2,Atlas Antibodies,HPA024452 Rabbit

(2) Progerin-induced nuclear envelope remodeling is shaped by cell division and NUP153Journal of Cell ScienceJune 15, 2026Ayse Mubine Turkmen, Jing Wang, Adam Frost, Katharine S. Ullmanabcam), anti-SUN2 (supernatant; 3.1E; a kind gift from Brian Burke) and anti-tubulin alpha (1:500; MCA77G; Bio-Rad). The secondary antibodies used are AlexaFluor-conjugated antibodies (ThermoFisher). Coverslips

(3) Structure and function of the nervous system in the stem of the siphonophore Nanomia septata : its role in swimming coordinationJournal of Experimental BiologyJune 1, 2026Tigran P. Norekian, Robert W. MeechTherefore, as a first marker, rat monoclonal anti-tubulin antibody was used (AbD Serotec, Bio-Rad, cat. no. MCA77G, RRID: AB_325003), which recognizes the alpha subunit of α-tubulin, specifically binding tyrosylated

(4) Mad1 facilitates α5 integrin trafficking from the Golgi to promote abscission during cytokinesisNature CommunicationsMarch 30, 2026Daniel K. Sam, Greta Grems, Anjon Audhya, Beth A. WeaverDevelopmental Studies Hybridoma Bank, 1:2000 for immunoblotting or 1:500 for IF), rat anti-alpha tubulin (MCA77G, clone YL1/2, BioRad, 1:700), Phalloidin (ActinGreen 488 ReadyProbes, Thermo Fisher, used according

(5) Endogenous and environmental factors that induce DNA replication defects and genomic instability in ER-negative heterozygous BRCA1 cellsScientific ReportsMarch 27, 2026Madhura Deshpande, Theodore Paniza, Rebecca Brown, Kate Heslin, Nitya Patel, Advaitha Madireddy, Zev Rosenwaks, Jeannine GerhardtPhospho-Histone H2A.X (1:1000, Cell signaling technology, # 9718 T), Tubulin Alpha (1:100, BioRad, # MCA77G), Vimentin (1:100, Cell Signaling Technology, # 5741 T). The slides were mounted with ProLong Gold Antifade

(6) Immunofluorescent microscopy to quantify and characterize chromosomal instability (CIN)Methods in Cell BiologyFebruary 13, 2026Kathryn M. Jones, Yeseo Choi, Radchanon Leelasukseree, Nikki N. Messick, Ava Bryan, Sophie Bice, Pippa F. Cosper5)Methanol (#A412P-4, Fisher Scientific)DAPI (#76482–850, VWR)Alpha tubulin YL1/2 antibody in rat (#MCA77G, BioRad)Centrin antibody in mouse (#04–1624, Millipore Sigma)Pericentrin antibody in rabbit (#ab4448,

(7) Accessibility of the unstructured α-tubulin C-terminal tail is controlled by microtubule lattice conformationeLifeFebruary 9, 2026Takashi Hotta, Morgan L Pimm, Ezekiel C Thomas, Yang Yue, Patrick DeLear, Lynne Blasius, Michael A Cianfrocco, Morgan E DeSantis, Ryota Horiuchi, Takumi Higaki, et al.WB, 0.5 µg/ml IF" Antibody,"Anti-tyrosinated α-tubulin, rat monoclonal, clone YL1/2",Bio-Rad,Cat# MCA77G,1:1000 WB Antibody,rMAb-YL1/2-EGFP recombinant protein,This study,,1:1000 WB; Verhey lab Antibody,"Anti-α-tubulin,

(8) Accessibility of the unstructured α-tubulin C-terminal tail is controlled by microtubule lattice conformationElifeFebruary 9, 2026Takashi Hotta, Morgan L Pimm, Ezekiel C Thomas, Yang Yue, Patrick DeLear, Lynne Blasius, Michael A Cianfrocco, Morgan E DeSantis, Ryota Horiuchi, Takumi Higaki, et al.WB, 0.5 µg/ml IF" Antibody,"Anti-tyrosinated α-tubulin, rat monoclonal, clone YL1/2",Bio-Rad,Cat# MCA77G,1:1000 WB Antibody,rMAb-YL1/2-EGFP recombinant protein,This study,,1:1000 WB; Verhey lab Antibody,"Anti-α-tubulin,

(9) Identification of MRVI1-interacting proteins by biotin-based proximity labelling reveals NPM-ALK-dependent interaction dynamicsJournal of biochemistryDecember 2, 2025Higashi K, Tanaka Y, Kosako H, Aoyama K.Laboratories; sc-271,944, Santa Cruz), anti-ALK (#3633, Cell Signalling Technology), anti-Tubulin (#MCA77G, Bio-Rad), Anti-Mouse IgG-HRP (#NA931, GE Healthcare; Light Chain Specific, #115–035-174, Jackson),

(10) Designing protein-based artificial kinetochores as decoys to prevent meiotic errors in oocytesNature Cell BiologyNovember 4, 2025Yuanzhuo Zhou, Kohei Asai, Hirohisa Kyogoku, Tomoya S. Kitajimarabbit anti-HURP (Santa Cruz Biotechnology, sc-98809; 1:500) and rat anti-α tubulin (YL1/2) (Bio-Rad, MCA77G; 1:1500). The secondary antibody used was Alexa Fluor 488 goat anti-Human IgG (H + L) (A11013), Alexa

(11) Understanding off-target growth defects introduced to influenza A virus by synonymous recodingRNANovember 1, 2025Colin P. Sharp, Beth H. Thompson, Ananya Ferdous Hoque, Ola Diebold, Blanka Tesla, Dominic Kurian, Peter Simmonds, Paul Digard, Eleanor Gauntvolume reactions (Promega, #L4960). For western blotting, antibody α-tubulin (clone YL1/2, Bio-Rad, #MCA77G) was used. PCR amplicon purification was performed using the PureLink PCR purification kit (Thermo Fisher Scientific

(12) The ribonuclease DISL-2 mediates piRNA degradation in C. elegansCell ReportsOctober 28, 2025Benjamin Pastore, Hannah L. Hertz, Wen TangRRID:AB_2910145 Mouse mAb anti-GFP,Roche,Cat# 11814460001; RRID:AB_390913 Rat pAb anti-alpha-tubulin,Bio-Rad,Cat# MCA77G; RRID:AB_325003 Rabbit mAb anti-mCherry,Cell Signaling Technology,Cat# 43590; RRID:AB_2799246 Goat anti-Rabbit IgG H&L (HRP

(13) Interactions of Antibody Drug Conjugate Anti‐Tubulin and Topoisomerase I Inhibitor Payloads with Radiotherapy to Potentiate ImmunotherapyAdvanced ScienceOctober 24, 2025Jacqueline Lesperance, Bryan S. Yung, Michael M. Allevato, Marcus M Cheng, Maria F. Camargo, Robert Saddawi‐Konefka, Kanika Dhawan, Ashwyn K. Sharma, Mahsa Mortaja, Sophie Bice, et al.phosphate buffered saline) for 1 h at room temperature then incubated in rat anti‐tubulin (YL1/2, Bio‐Rad, MCA77G) diluted 1:1000 in Triton Block for 1 h. Coverslips were washed 3×5 min with 0.1% Triton X‐100 in PBS

(14) A Human Neural Tube Model Using 4D Self‐Folding Smart ScaffoldsAdvanced Healthcare MaterialsOctober 23, 2025Claudia Dell'Amico, Irene Chiesa, Angela Toffano, Alessio Esposito, Piera Mancini, Chiara Magliaro, Angeliki Louvi, Carmelo De Maria, Marco OnoratiBioscience FOXG1,1:500,ab18259,Abcam GOLGA2,1:200,A‐21270,Invitrogen Nanog,1:100,09‐0020,Stemgent Nestin,1:200,MAB1259,RD OCT4,1:100,09‐0023,Stemgent PAX6,1:100,901301,Biolegend PCTN,1:1000,ab28144,Abcam Phalloidin‐488,1:400,a12379,Thermo Fisher SOX2,1:400,ab5603,Millipore TJP1,1:100,617300,Invitrogen TUBA1A,1:1000,mca77g,Bio‐rad TUBB3,1:1000,ab41489,Abcam WDR62,1:500,A301‐560A,Bethyl Lab List of the antibodies and

(15) Integration of focal adhesion morphogenesis and polarity by DOCK5 promotes YAP/TAZ-driven drug resistance in TNBC†Molecular OmicsSeptember 22, 2025Patricia Pascual-Vargas, Mar Arias-Garcia, Theodoros I. Roumeliotis, Jyoti S. Choudhary, Chris BakalAntibodies used were: mouse YAP/TAZ (Santa Cruz Biotechnologies, cat#sc-101199), rat α-tubulin (Bio-Rad, cat#MCA77G), AlexaFluor 647 goat anti-mouse (Life Technologies, cat#A21235), AlexaFluor 647 goat anti-rat (Life Technologies

(16) Kif11-haploinsufficient oocytes reveal spatially differential requirements for chromosome biorientationEMBO ReportsAugust 20, 2025Tappei Mishina, Aurélien Courtois, Shuhei Yoshida, Kohei Asai, Hiroshi Kiyonari, Tomoya S KitajimaBioproducts), a rabbit anti-KIF11 (1:500, HPA010568, Sigma), and a rat monoclonal anti-alpha tubulin (1:2000, MCA77G, Bio-Rad). The secondary antibodies were Alexa Fluor 488 goat anti-rabbit IgG (H + L) (A11034); Alexa

(17) MPS1 promotes timely spindle bipolarization to prevent kinetochore-microtubule attachment errors in oocytesThe EMBO JournalJune 4, 2025Shuhei Yoshida, Reiko Nakagawa, Kohei Asai, Tomoya S Kitajimaantibody (ACA, 1:500, 15-234, Antibodies Incorporated), a rat monoclonal anti-alpha tubulin (1:2000, MCA77G, Bio-Rad), a rat anti-GFP antibody (1:500, GF090R 04404-84, Nacalai), and a rabbit anti-PRC1 (1:100,

(18) Lepidopteran scale cells derive from sensory organ precursors through a canonical lineageDevelopmentMarch 1, 2025Ling S. Loh, Kyle A. DeMarr, Martina Tsimba, Christa Heryanto, Alejandro Berrio, Nipam H. Patel, Arnaud Martin, W. Owen McMillan, Gregory A. Wray, Joseph J. HanlyCenter (GWNIC). Antibodies used for immunofluorescence were: rat monoclonal anti-tubulin (Bio-Rad, MCA77G; 1:100), rabbit polyclonal anti-Senseless (gift from the Perry lab, University of California San Diego,

(19) Doublecortin reinforces microtubules to promote growth cone advance in soft environmentsCurrent BiologyDecember 16, 2024Alessandro Dema, Rabab A. Charafeddine, Jeffrey van Haren, Shima Rahgozar, Giulia Viola, Kyle A. Jacobs, Matthew L. Kutys, Torsten Wittmannanti-MAPT(tau) E-4,Santa Cruz Biotechnology,sc-515539 Rat monoclonal anti-Tubulin (clone YL1/2),Bio-rad,MCA77G (RRID: AB_325003) "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant

(20) Making Neurobots and Chimerical CtenophoresbioRxivOctober 31, 2024Leonid L Moroz, Tigran P. Norekian6% goat serum in PBS and then for 48 hours in the primary anti-tubulin antibody (Bio-Rad, Catalog# MCA77G, RRID: AB_325003) diluted in blocking solution at a final dilution 1:50 in a refrigerator at 5°C. Following

Be the First to Write a Review!

RAT ANTI TUBULIN ALPHA

RAT ANTI TUBULIN ALPHA

RAT ANTI TUBULIN ALPHA

RAT ANTI TUBULIN ALPHA from Bio-Rad (AbD Serotec - FAB)

Questions and Answers

Bio-Rad

Citations (145)

Reviews

RAT ANTI TUBULIN ALPHA from Bio-Rad (AbD Serotec - FAB)

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map